Restriction map for
pF1KE3822
(Circular) (Six-base) MAPSORT of: pF1KE3822.seq Check: 4137 from: 1 to: 10694
pF1KE3822 10694 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 333 333
Size: 10694
AccI GT'mk_AC
Cuts at: 7184 7434 7732 7184
Size: 250 298 10146
Fragments arranged by size:
10146 298 250
Acc65I G'GTAC_C
Cuts at: 7710 7710
Size: 10694
AclI AA'CG_TT
Cuts at: 10534 10534
Size: 10694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 524 535 1496 2723 2954 2965 3229 3511
Size: 349 11 961 1227 231 11 264 282
Cuts at: 3511 3520 4406 5360 5384 5968 6251 6686 7348
Size: 9 886 954 24 584 283 435 662
Cuts at: 7348 7568 8519 8951 9698 9966 175
Size: 220 951 432 747 268 903
Fragments arranged by size:
1227 961 954 951 903 886 747 662
584 435 432 349 283 282 268 264
231 220 24 11 11 9
AfeI AGC'GCT
Cuts at: 1043 1043
Size: 10694
AflII C'TTAA_G
Cuts at: 2478 3771 2478
Size: 1293 9401
AflIII A'CryG_T
Cuts at: 660 5042 7443 8046 9150 10127 660
Size: 4382 2401 603 1104 977 1227
Fragments arranged by size:
4382 2401 1227 1104 977 603
AgeI A'CCGG_T
Cuts at: 9082 9082
Size: 10694
AleI CACnn'nnGTG
Cuts at: 360 3418 360
Size: 3058 7636
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 6044 6044
Size: 10694
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3646 3678 3646
Size: 32 10662
AlwNI CAG_nnn'CTG
Cuts at: 1920 7999 9566 1920
Size: 6079 1567 3048
Fragments arranged by size:
6079 3048 1567
ApaI G_GGCC'C
Cuts at: 2921 2921
Size: 10694
ApaLI G'TGCA_C
Cuts at: 6224 7276 9464 6224
Size: 1052 2188 7454
Fragments arranged by size:
7454 2188 1052
ApoI r'AATT_y
Cuts at: 700 747 902 1681 1910 1994 2694 2719 2851
Size: 47 155 779 229 84 700 25 132
Cuts at: 2851 2889 3244 3594 3658 4306 4738 5005 5026
Size: 38 355 350 64 648 432 267 21
Cuts at: 5026 5071 5734 6504 6888 7029 7623 7698 10047
Size: 45 663 770 384 141 594 75 2349
Cuts at: 10047 10303 700
Size: 256 1091
Fragments arranged by size:
2349 1091 779 770 700 663 648 594
432 384 355 350 267 256 229 155
141 132 84 75 64 47 45 38
25 21
AseI AT'TA_AT
Cuts at: 20 370 4168 5230 6436 20
Size: 350 3798 1062 1206 4278
Fragments arranged by size:
4278 3798 1206 1062 350
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10694
AvaI C'yCGr_G
Cuts at: 7714 7714
Size: 10694
AvrII C'CTAG_G
Cuts at: 5760 5760
Size: 10694
BamHI G'GATC_C
Cuts at: 7719 7719
Size: 10694
BanI G'GyrC_C
Cuts at: 7710 8124 8372 8407 7710
Size: 414 248 35 9997
Fragments arranged by size:
9997 414 248 35
BanII G_rGCy'C
Cuts at: 2921 7708 8738 2921
Size: 4787 1030 4877
Fragments arranged by size:
4877 4787 1030
BbeI G_GCGC'C
Cuts at: 8128 8376 8128
Size: 248 10446
BbsI GAAGACnn'nnnn_
Cuts at: 523 2138 523
Size: 1615 9079
BbvCI CC'TCA_GC
Cuts at: 4369 4369
Size: 10694
BciVI GTATCCnnnnn_n'
Cuts at: 1668 1904 1933 6134 6664 8592 9353 10694 1668
Size: 236 29 4201 530 1928 761 1341 1668
Fragments arranged by size:
4201 1928 1668 1341 761 530 236 29
BclI T'GATC_A
Cuts at: 2259 2636 2928 8214 2259
Size: 377 292 5286 4739
Fragments arranged by size:
5286 4739 377 292
BfrBI ATG'CAT
Cuts at: 1329 1369 1834 6569 1329
Size: 40 465 4735 5454
Fragments arranged by size:
5454 4735 465 40
BglII A'GATC_T
Cuts at: 525 1034 4188 4266 5619 8209 10246 525
Size: 509 3154 78 1353 2590 2037 973
Fragments arranged by size:
3154 2590 2037 1353 973 509 78
BlpI GC'TnA_GC
Cuts at: 1045 6711 7205 7806 1045
Size: 5666 494 601 3933
Fragments arranged by size:
5666 3933 601 494
Bme1580I G_kGCm'C
Cuts at: 1227 2921 6211 6228 6414 6626 7280 8319 8412
Size: 1694 3290 17 186 212 654 1039 93
Cuts at: 8412 9468 1227
Size: 1056 2453
Fragments arranged by size:
3290 2453 1694 1056 1039 654 212 186
93 17
BmrI ACTGGGnnnn_n'
Cuts at: 4145 5891 7906 7987 8322 10466 4145
Size: 1746 2015 81 335 2144 4373
Fragments arranged by size:
4373 2144 2015 1746 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 276 696 5973 6658 9061 276
Size: 420 5277 685 2403 1909
Fragments arranged by size:
5277 2403 1909 685 420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 1633 3175 3454 5515 5732 6335 7415 244
Size: 1389 1542 279 2061 217 603 1080 3523
Fragments arranged by size:
3523 2061 1542 1389 1080 603 279 217
Bpu10I CC'TnA_GC
Cuts at: 3106 4369 3106
Size: 1263 9431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3504 3574 3837 5266 6449 7870 8749 9241 9539
Size: 70 263 1429 1183 1421 879 492 298
Cuts at: 9539 3504
Size: 4659
Fragments arranged by size:
4659 1429 1421 1183 879 492 298 263
70
BsaI GGTCTCn'nnnn_
Cuts at: 5339 6080 10398 5339
Size: 741 4318 5635
Fragments arranged by size:
5635 4318 741
BsaAI yAC'GTr
Cuts at: 245 1203 6039 7444 8047 245
Size: 958 4836 1405 603 2892
Fragments arranged by size:
4836 2892 1405 958 603
BsaBI GATnn'nnATC
Cuts at: 6 2312 3805 8208 10245 6
Size: 2306 1493 4403 2037 455
Fragments arranged by size:
4403 2306 2037 1493 455
BsaHI Gr'CG_yC
Cuts at: 8125 8373 9075 10571 8125
Size: 248 702 1496 8248
Fragments arranged by size:
8248 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4754 7535 7883 8404 9082 9356 9503 9914 4754
Size: 2781 348 521 678 274 147 411 5534
Fragments arranged by size:
5534 2781 678 521 411 348 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 5798 5828 5798
Size: 30 10664
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 520 700 1051 7412 520
Size: 180 351 6361 3802
Fragments arranged by size:
6361 3802 351 180
BseYI C'CCAG_C
Cuts at: 1220 1293 4869 5920 6612 8119 8877 9454 9950
Size: 73 3576 1051 692 1507 758 577 496
Cuts at: 9950 1220
Size: 1964
Fragments arranged by size:
3576 1964 1507 1051 758 692 577 496
73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 1024 1225 1627 358
Size: 666 201 402 9425
Fragments arranged by size:
9425 666 402 201
BsiEI CG_ry'CG
Cuts at: 104 8236 8282 9490 104
Size: 8132 46 1208 1308
Fragments arranged by size:
8132 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 257 5286 6228 7280 7708 8486 8676 9468 257
Size: 5029 942 1052 428 778 190 792 1483
Fragments arranged by size:
5029 1483 1052 942 792 778 428 190
BsmI GAATG_Cn'
Cuts at: 883 1026 1329 1371 2290 3015 3174 4202 4578
Size: 143 303 42 919 725 159 1028 376
Cuts at: 4578 6571 6603 883
Size: 1993 32 4974
Fragments arranged by size:
4974 1993 1028 919 725 376 303 159
143 42 32
BsmBI CGTCTCn'nnnn_
Cuts at: 6525 7665 6525
Size: 1140 9554
Bsp1286I G_dGCh'C
Cuts at: 257 1227 2921 5286 6211 6228 6414 6626 7280
Size: 970 1694 2365 925 17 186 212 654
Cuts at: 7280 7708 8319 8412 8486 8676 8738 9468 257
Size: 428 611 93 74 190 62 730 1483
Fragments arranged by size:
2365 1694 1483 970 925 730 654 611
428 212 190 186 93 74 62 17
BspEI T'CCGG_A
Cuts at: 9914 9914
Size: 10694
BspHI T'CATG_A
Cuts at: 833 1587 2931 7478 9870 833
Size: 754 1344 4547 2392 1657
Fragments arranged by size:
4547 2392 1657 1344 754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 333 4285 5705 7744 7958 8260 8641 333
Size: 3952 1420 2039 214 302 381 2386
Fragments arranged by size:
3952 2386 2039 1420 381 302 214
BsrBI CCG'CTC
Cuts at: 50 8986 9040 10524 10689 50
Size: 8936 54 1484 165 55
Fragments arranged by size:
8936 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 849 1027 3729 6140 6164 8606 849
Size: 178 2702 2411 24 2442 2937
Fragments arranged by size:
2937 2702 2442 2411 178 24
BsrFI r'CCGG_y
Cuts at: 8692 8873 9082 8692
Size: 181 209 10304
Fragments arranged by size:
10304 209 181
BsrGI T'GTAC_A
Cuts at: 1818 1818
Size: 10694
BssSI C'ACGA_G
Cuts at: 2405 3177 8965 9323 2405
Size: 772 5788 358 3776
Fragments arranged by size:
5788 3776 772 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4499 6151 8071 4499
Size: 1652 1920 7122
Fragments arranged by size:
7122 1920 1652
BstBI TT'CG_AA
Cuts at: 9055 9055
Size: 10694
BstXI CCAn_nnnn'nTGG
Cuts at: 421 643 2157 2379 2913 3969 421
Size: 222 1514 222 534 1056 7146
Fragments arranged by size:
7146 1514 1056 534 222 222
BstYI r'GATC_y
Cuts at: 1 228 525 1034 2329 2401 3336 3952 3993
Size: 227 297 509 1295 72 935 616 41
Cuts at: 3993 4188 4266 4643 5359 5619 7419 7719 8186
Size: 195 78 377 716 260 1800 300 467
Cuts at: 8186 8209 8544 8790 9802 9888 9900 10246 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1800 1295 1012 935 716 616 509 467
449 377 346 335 300 297 260 246
227 195 86 78 72 41 23 12
Bsu36I CC'TnA_GG
Cuts at: 2679 4973 2679
Size: 2294 8400
BtgI C'CryG_G
Cuts at: 107 6415 107
Size: 6308 4386
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2702 8644 8825 8988 2702
Size: 5942 181 163 4408
Fragments arranged by size:
5942 4408 181 163
BtsI GCAGTG_nn'
Cuts at: 1559 1882 4873 7187 7373 7937 1559
Size: 323 2991 2314 186 564 4316
Fragments arranged by size:
4316 2991 2314 564 323 186
ClaI AT'CG_AT
Cuts at: 5238 5238
Size: 10694
DraI TTT'AAA
Cuts at: 753 3509 3686 4032 7693 753
Size: 2756 177 346 3661 3754
Fragments arranged by size:
3754 3661 2756 346 177
DraIII CAC_nnn'GTG
Cuts at: 4569 6105 4569
Size: 1536 9158
DrdI GACnn_nn'nnGTC
Cuts at: 8400 9258 8400
Size: 858 9836
EaeI y'GGCC_r
Cuts at: 1284 2501 8279 8453 8844 8871 10216 1284
Size: 1217 5778 174 391 27 1345 1762
Fragments arranged by size:
5778 1762 1345 1217 391 174 27
EagI C'GGCC_G
Cuts at: 8279 8279
Size: 10694
EarI CTCTTCn'nnn_
Cuts at: 131 755 2754 3205 4417 8717 8927 131
Size: 624 1999 451 1212 4300 210 1898
Fragments arranged by size:
4300 1999 1898 1212 624 451 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 8902 9212 9358 10504 8902
Size: 310 146 1146 9092
Fragments arranged by size:
9092 1146 310 146
EcoICRI GAG'CTC
Cuts at: 7706 7706
Size: 10694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 244 524 535 1496 1633 2723 2954 2965
Size: 69 280 11 961 137 1090 231 11
Cuts at: 2965 3175 3229 3454 3511 3520 4406 5360 5384
Size: 210 54 225 57 9 886 954 24
Cuts at: 5384 5515 5732 5968 6251 6335 6686 7348 7415
Size: 131 217 236 283 84 351 662 67
Cuts at: 7415 7568 8519 8951 9698 9966 175
Size: 153 951 432 747 268 903
Fragments arranged by size:
1090 961 954 951 903 886 747 662
432 351 283 280 268 236 231 225
217 210 153 137 131 84 69 67
57 54 24 11 11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 4372 4372
Size: 10694
EcoO109I rG'GnC_Cy
Cuts at: 409 2917 3745 7833 409
Size: 2508 828 4088 3270
Fragments arranged by size:
4088 3270 2508 828
EcoRI G'AATT_C
Cuts at: 902 4306 6504 7698 902
Size: 3404 2198 1194 3898
Fragments arranged by size:
3898 3404 2198 1194
EcoRV GAT'ATC
Cuts at: 2644 5906 2644
Size: 3262 7432
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1547 5985 6494 8713 1547
Size: 4438 509 2219 3528
Fragments arranged by size:
4438 3528 2219 509
FspI TGC'GCA
Cuts at: 8475 8475
Size: 10694
HaeII r_GCGC'y
Cuts at: 1045 1621 8128 8376 9398 10377 1045
Size: 576 6507 248 1022 979 1362
Fragments arranged by size:
6507 1362 1022 979 576 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 510 542 1831 1863 2628 2660 2698 2730 3888
Size: 32 1289 32 765 32 38 32 1158
Cuts at: 3888 3920 4256 4288 4635 4667 4770 4802 5351
Size: 32 336 32 347 32 103 32 549
Cuts at: 5351 5383 6233 6265 7561 7593 8536 8566 8568
Size: 32 850 32 1296 32 943 30 2
Cuts at: 8568 8598 510
Size: 30 2606
Fragments arranged by size:
2606 1296 1289 1158 943 850 765 549
347 336 103 38 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 743 1376 2432 7435 7733 743
Size: 633 1056 5003 298 3704
Fragments arranged by size:
5003 3704 1056 633 298
HindIII A'AGCT_T
Cuts at: 399 653 5342 6949 399
Size: 254 4689 1607 4144
Fragments arranged by size:
4689 4144 1607 254
HpaI GTT'AAC
Cuts at: 743 743
Size: 10694
KasI G'GCGC_C
Cuts at: 8124 8372 8124
Size: 248 10446
KpnI G_GTAC'C
Cuts at: 7714 7714
Size: 10694
MfeI C'AATT_G
Cuts at: 454 4733 454
Size: 4279 6415
MluI A'CGCG_T
Cuts at: 10127 10127
Size: 10694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 402 3379 4644 5208 5711 6882 7187 9365 9549
Size: 2977 1265 564 503 1171 305 2178 184
Cuts at: 9549 402
Size: 1547
Fragments arranged by size:
2977 2178 1547 1265 1171 564 503 305
184
MscI TGG'CCA
Cuts at: 1286 2503 8455 1286
Size: 1217 5952 3525
Fragments arranged by size:
5952 3525 1217
MslI CAynn'nnrTG
Cuts at: 360 1179 1324 1436 3167 3418 3834 4222 4469
Size: 819 145 112 1731 251 416 388 247
Cuts at: 4469 7008 7174 7642 8683 8810 360
Size: 2539 166 468 1041 127 2244
Fragments arranged by size:
2539 2244 1731 1041 819 468 416 388
251 247 166 145 127 112
MspA1I CmG'CkG
Cuts at: 122 1049 1611 3390 4671 4791 5920 6511 6910
Size: 927 562 1779 1281 120 1129 591 399
Cuts at: 6910 7805 8119 8479 9492 9737 122
Size: 895 314 360 1013 245 1079
Fragments arranged by size:
1779 1281 1129 1079 1013 927 895 591
562 399 360 314 245 120
NaeI GCC'GGC
Cuts at: 8875 8875
Size: 10694
NarI GG'CG_CC
Cuts at: 8125 8373 8125
Size: 248 10446
NcoI C'CATG_G
Cuts at: 107 6415 107
Size: 6308 4386
NdeI CA'TA_TG
Cuts at: 3008 3008
Size: 10694
NgoMIV G'CCGG_C
Cuts at: 8873 8873
Size: 10694
NruI TCG'CGA
Cuts at: 331 331
Size: 10694
NsiI A_TGCA'T
Cuts at: 1331 1371 1836 6571 1331
Size: 40 465 4735 5454
Fragments arranged by size:
5454 4735 465 40
NspI r_CATG'y
Cuts at: 664 1220 1656 5046 5265 7747 8247 9154 664
Size: 556 436 3390 219 2482 500 907 2204
Fragments arranged by size:
3390 2482 2204 907 556 500 436 219
PacI TTA_AT'TAA
Cuts at: 6440 6440
Size: 10694
PciI A'CATG_T
Cuts at: 660 5042 9150 660
Size: 4382 4108 2204
Fragments arranged by size:
4382 4108 2204
PflMI CCAn_nnn'nTGG
Cuts at: 222 5448 222
Size: 5226 5468
PfoI T'CCnGG_A
Cuts at: 185 221 7415 185
Size: 36 7194 3464
Fragments arranged by size:
7194 3464 36
PmeI GTTT'AAAC
Cuts at: 7693 7693
Size: 10694
PmlI CAC'GTG
Cuts at: 1203 1203
Size: 10694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9833 9865 9833
Size: 32 10662
PpuMI rG'GwC_Cy
Cuts at: 409 3745 409
Size: 3336 7358
PsiI TTA'TAA
Cuts at: 857 4103 6257 857
Size: 3246 2154 5294
Fragments arranged by size:
5294 3246 2154
PspOMI G'GGCC_C
Cuts at: 2917 2917
Size: 10694
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2402 2434 2402
Size: 32 10662
PstI C_TGCA'G
Cuts at: 1889 4796 4880 7741 8426 1889
Size: 2907 84 2861 685 4157
Fragments arranged by size:
4157 2907 2861 685 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10694
PvuII CAG'CTG
Cuts at: 122 1049 1611 3390 4671 4791 5920 6511 8119
Size: 927 562 1779 1281 120 1129 591 1608
Cuts at: 8119 8479 122
Size: 360 2337
Fragments arranged by size:
2337 1779 1608 1281 1129 927 591 562
360 120
RsrII CG'GwC_CG
Cuts at: 8889 8889
Size: 10694
SacI G_AGCT'C
Cuts at: 7708 7708
Size: 10694
SalI G'TCGA_C
Cuts at: 7433 7731 7433
Size: 298 10396
SanDI GG'GwC_CC
Cuts at: 409 409
Size: 10694
SapI GCTCTTCn'nnn_
Cuts at: 4417 8717 8927 4417
Size: 4300 210 6184
Fragments arranged by size:
6184 4300 210
SbfI CC_TGCA'GG
Cuts at: 7741 7741
Size: 10694
ScaI AGT'ACT
Cuts at: 4317 6175 6999 10211 4317
Size: 1858 824 3212 4800
Fragments arranged by size:
4800 3212 1858 824
SexAI A'CCwGG_T
Cuts at: 3741 3741
Size: 10694
SfcI C'TryA_G
Cuts at: 33 1885 3341 4311 4792 4876 6016 6180 7737
Size: 1852 1456 970 481 84 1140 164 1557
Cuts at: 7737 8422 9415 9606 33
Size: 685 993 191 1121
Fragments arranged by size:
1852 1557 1456 1140 1121 993 970 685
481 191 164 84
SfoI GGC'GCC
Cuts at: 8126 8374 8126
Size: 248 10446
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10694
SmaI CCC'GGG
Cuts at: 7716 7716
Size: 10694
SmlI C'TyrA_G
Cuts at: 2478 3483 3553 3771 3816 5245 6464 7849 8764
Size: 1005 70 218 45 1429 1219 1385 915
Cuts at: 8764 9256 9518 2478
Size: 492 262 3654
Fragments arranged by size:
3654 1429 1385 1219 1005 915 492 262
218 70 45
SpeI A'CTAG_T
Cuts at: 67 4773 67
Size: 4706 5988
SphI G_CATG'C
Cuts at: 1220 1656 7747 8247 1220
Size: 436 6091 500 3667
Fragments arranged by size:
6091 3667 500 436
SspI AAT'ATT
Cuts at: 861 2132 2963 5104 5709 6704 861
Size: 1271 831 2141 605 995 4851
Fragments arranged by size:
4851 2141 1271 995 831 605
StuI AGG'CCT
Cuts at: 5139 6474 5139
Size: 1335 9359
StyI C'CwwG_G
Cuts at: 107 441 2520 2912 4405 4517 5644 5760 5960
Size: 334 2079 392 1493 112 1127 116 200
Cuts at: 5960 5969 6415 6716 7828 8182 107
Size: 9 446 301 1112 354 2619
Fragments arranged by size:
2619 2079 1493 1127 1112 446 392 354
334 301 200 116 112 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 7458 8277 8410 8869 9078 7458
Size: 819 133 459 209 9074
Fragments arranged by size:
9074 819 459 209 133
TatI w'GTAC_w
Cuts at: 1818 4315 4892 6173 6997 10209 1818
Size: 2497 577 1281 824 3212 2303
Fragments arranged by size:
3212 2497 2303 1281 824 577
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3168 3200 3311 3343 3517 3549 4894 4926 5798
Size: 32 111 32 174 32 1345 32 872
Cuts at: 5798 5830 6108 6140 10211 10243 3168
Size: 32 278 32 4071 32 3619
Fragments arranged by size:
4071 3619 1345 872 278 174 111 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6765 8491 6765
Size: 1726 8968
XbaI T'CTAG_A
Cuts at: 3841 7725 3841
Size: 3884 6810
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5216 5967 6043 8189 5216
Size: 751 76 2146 7721
Fragments arranged by size:
7721 2146 751 76
XmaI C'CCGG_G
Cuts at: 7714 7714
Size: 10694
XmnI GAAnn'nnTTC
Cuts at: 883 902 1762 1963 2697 4983 5006 5480 883
Size: 19 860 201 734 2286 23 474 6097
Fragments arranged by size:
6097 2286 860 734 474 201 23 19
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AscI BaeI BcgI BglI BmgBI BmtI
BsiWI BssHII BstEII BstZ17I CspCI FseI FspAI NheI
NotI PasI PshAI PspXI SacII SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI