Restriction map for
pF1KE3818
(Circular) (Six-base) MAPSORT of: pF1KE3818.seq Check: 5687 from: 1 to: 6659
pF1KE3818 6659 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 108 108
Size: 6659
AatII G_ACGT'C
Cuts at: 1511 1511
Size: 6659
AccI GT'mk_AC
Cuts at: 3437 3697 3437
Size: 260 6399
Acc65I G'GTAC_C
Cuts at: 626 3675 626
Size: 3049 3610
AclI AA'CG_TT
Cuts at: 6499 6499
Size: 6659
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 804 1775 1873 2049 3551 4484 4916 5663 5931
Size: 971 98 176 1502 933 432 747 268
Cuts at: 5931 804
Size: 1532
Fragments arranged by size:
1532 1502 971 933 747 432 268 176
98
AflII C'TTAA_G
Cuts at: 1395 1395
Size: 6659
AflIII A'CryG_T
Cuts at: 753 4011 5115 6092 753
Size: 3258 1104 977 1320
Fragments arranged by size:
3258 1320 1104 977
AgeI A'CCGG_T
Cuts at: 5047 5047
Size: 6659
AhdI GACnn_n'nnGTC
Cuts at: 3393 3393
Size: 6659
AleI CACnn'nnGTG
Cuts at: 598 900 2253 598
Size: 302 1353 5004
Fragments arranged by size:
5004 1353 302
AlwNI CAG_nnn'CTG
Cuts at: 862 1051 2519 3574 3577 3964 5531 862
Size: 189 1468 1055 3 387 1567 1990
Fragments arranged by size:
1990 1567 1468 1055 387 189 3
ApaLI G'TGCA_C
Cuts at: 1946 3401 5429 1946
Size: 1455 2028 3176
Fragments arranged by size:
3176 2028 1455
ApoI r'AATT_y
Cuts at: 1090 1209 1317 1444 1908 2977 3505 3663 6012
Size: 119 108 127 464 1069 528 158 2349
Cuts at: 6012 6268 1090
Size: 256 1481
Fragments arranged by size:
2349 1481 1069 528 464 256 158 127
119 108
AseI AT'TA_AT
Cuts at: 20 20
Size: 6659
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6659
AvaI C'yCGr_G
Cuts at: 3586 3679 3586
Size: 93 6566
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 742 775 3290 3323 742
Size: 33 2515 33 4078
Fragments arranged by size:
4078 2515 33 33
BamHI G'GATC_C
Cuts at: 170 1963 3684 170
Size: 1793 1721 3145
Fragments arranged by size:
3145 1793 1721
BanI G'GyrC_C
Cuts at: 576 626 2244 3675 4089 4337 4372 576
Size: 50 1618 1431 414 248 35 2863
Fragments arranged by size:
2863 1618 1431 414 248 50 35
BanII G_rGCy'C
Cuts at: 149 397 1684 3673 4703 149
Size: 248 1287 1989 1030 2105
Fragments arranged by size:
2105 1989 1287 1030 248
BbeI G_GCGC'C
Cuts at: 4093 4341 4093
Size: 248 6411
BbvCI CC'TCA_GC
Cuts at: 158 1766 158
Size: 1608 5051
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2226 2260 2226
Size: 34 6625
BciVI GTATCCnnnnn_n'
Cuts at: 4557 5318 6659 4557
Size: 761 1341 4557
Fragments arranged by size:
4557 1341 761
BclI T'GATC_A
Cuts at: 264 4179 264
Size: 3915 2744
BglI GCCn_nnn'nGGC
Cuts at: 2844 3280 2844
Size: 436 6223
BglII A'GATC_T
Cuts at: 1332 4174 6211 1332
Size: 2842 2037 1780
Fragments arranged by size:
2842 2037 1780
BlpI GC'TnA_GC
Cuts at: 667 3771 667
Size: 3104 3555
Bme1580I G_kGCm'C
Cuts at: 1893 1950 2799 3057 3200 3405 3422 3587 4284
Size: 57 849 258 143 205 17 165 697
Cuts at: 4284 4377 5433 1893
Size: 93 1056 3119
Fragments arranged by size:
3119 1056 849 697 258 205 165 143
93 57 17
BmgBI CAC'GTC
Cuts at: 754 2943 754
Size: 2189 4470
BmrI ACTGGGnnnn_n'
Cuts at: 592 2398 3871 3952 4287 6431 592
Size: 1806 1473 81 335 2144 820
Fragments arranged by size:
2144 1806 1473 820 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 3360 5026 2108
Size: 1252 1666 3741
Fragments arranged by size:
3741 1666 1252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 332 1433 1457 1728 2521 2720 2774 2834
Size: 199 1101 24 271 793 199 54 60
Cuts at: 2834 2837 2918 3035 133
Size: 3 81 117 3757
Fragments arranged by size:
3757 1101 793 271 199 199 117 81
60 54 24 3
Bpu10I CC'TnA_GC
Cuts at: 158 739 1766 3468 158
Size: 581 1027 1702 3349
Fragments arranged by size:
3349 1702 1027 581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3224 3835 4714 5206 5504 3224
Size: 611 879 492 298 4379
Fragments arranged by size:
4379 879 611 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 229 2640 6363 229
Size: 2411 3723 525
Fragments arranged by size:
3723 2411 525
BsaAI yAC'GTr
Cuts at: 4012 4012
Size: 6659
BsaBI GATnn'nnATC
Cuts at: 6 4173 6210 6
Size: 4167 2037 455
Fragments arranged by size:
4167 2037 455
BsaHI Gr'CG_yC
Cuts at: 1022 1508 4090 4338 5040 6536 1022
Size: 486 2582 248 702 1496 1145
Fragments arranged by size:
2582 1496 1145 702 486 248
BsaWI w'CCGG_w
Cuts at: 3848 4369 5047 5321 5468 5879 3848
Size: 521 678 274 147 411 4628
Fragments arranged by size:
4628 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 835 865 835
Size: 30 6629
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1779 1783 2687 1779
Size: 4 904 5751
Fragments arranged by size:
5751 904 4
BseYI C'CCAG_C
Cuts at: 257 572 891 1968 2002 2225 2329 3214 4084
Size: 315 319 1077 34 223 104 885 870
Cuts at: 4084 4842 5419 5915 257
Size: 758 577 496 1001
Fragments arranged by size:
1077 1001 885 870 758 577 496 319
315 223 104 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 671 2965 3667 206
Size: 465 2294 702 3198
Fragments arranged by size:
3198 2294 702 465
BsiEI CG_ry'CG
Cuts at: 104 204 4201 4247 5455 104
Size: 100 3997 46 1208 1308
Fragments arranged by size:
3997 1308 1208 100 46
BsiHKAI G_wGCw'C
Cuts at: 397 1950 3405 3673 4451 4641 5433 397
Size: 1553 1455 268 778 190 792 1623
Fragments arranged by size:
1623 1553 1455 792 778 268 190
BsmI GAATG_Cn'
Cuts at: 1237 1237
Size: 6659
Bsp1286I G_dGCh'C
Cuts at: 149 397 1684 1893 1950 2799 3057 3200 3405
Size: 248 1287 209 57 849 258 143 205
Cuts at: 3405 3422 3587 3673 4284 4377 4451 4641 4703
Size: 17 165 86 611 93 74 190 62
Cuts at: 4703 5433 149
Size: 730 1375
Fragments arranged by size:
1375 1287 849 730 611 258 248 209
205 190 165 143 93 86 74 62
57 17
BspEI T'CCGG_A
Cuts at: 5879 5879
Size: 6659
BspHI T'CATG_A
Cuts at: 2075 5835 2075
Size: 3760 2899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 108 1820 2392 3286 3709 3923 4225 4606 108
Size: 1712 572 894 423 214 302 381 2161
Fragments arranged by size:
2161 1712 894 572 423 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4951 5005 6489 6654 50
Size: 4901 54 1484 165 55
Fragments arranged by size:
4901 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1301 2507 4571 1301
Size: 1206 2064 3389
Fragments arranged by size:
3389 2064 1206
BsrFI r'CCGG_y
Cuts at: 238 4657 4838 5047 238
Size: 4419 181 209 1850
Fragments arranged by size:
4419 1850 209 181
BsrGI T'GTAC_A
Cuts at: 840 3624 840
Size: 2784 3875
BssSI C'ACGA_G
Cuts at: 4930 5288 4930
Size: 358 6301
BstAPI GCAn_nnn'nTGC
Cuts at: 1897 4036 1897
Size: 2139 4520
BstBI TT'CG_AA
Cuts at: 5020 5020
Size: 6659
BstXI CCAn_nnnn'nTGG
Cuts at: 282 282
Size: 6659
BstYI r'GATC_y
Cuts at: 1 170 1332 1963 2709 3684 4151 4174 4509
Size: 169 1162 631 746 975 467 23 335
Cuts at: 4509 4755 5767 5853 5865 6211 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1162 1012 975 746 631 467 449 346
335 246 169 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1628 1643 2472 1628
Size: 15 829 5815
Fragments arranged by size:
5815 829 15
BtgI C'CryG_G
Cuts at: 107 165 630 1076 107
Size: 58 465 446 5690
Fragments arranged by size:
5690 465 446 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4609 4790 4953 4609
Size: 181 163 6315
Fragments arranged by size:
6315 181 163
BtsI GCAGTG_nn'
Cuts at: 189 654 779 1972 2324 2325 3200 3902 189
Size: 465 125 1193 352 1 875 702 2946
Fragments arranged by size:
2946 1193 875 702 465 352 125 1
DraI TTT'AAA
Cuts at: 3658 3658
Size: 6659
DraIII CAC_nnn'GTG
Cuts at: 649 2898 649
Size: 2249 4410
DrdI GACnn_nn'nnGTC
Cuts at: 4365 5223 4365
Size: 858 5801
EaeI y'GGCC_r
Cuts at: 201 1274 3281 4244 4418 4809 4836 6181 201
Size: 1073 2007 963 174 391 27 1345 679
Fragments arranged by size:
2007 1345 1073 963 679 391 174 27
EagI C'GGCC_G
Cuts at: 201 4244 201
Size: 4043 2616
EarI CTCTTCn'nnn_
Cuts at: 445 1238 1751 2077 2568 2570 2690 3113 4682
Size: 793 513 326 491 2 120 423 1569
Cuts at: 4682 4892 445
Size: 210 2212
Fragments arranged by size:
2212 1569 793 513 491 423 326 210
120 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4867 5177 5323 6469 4867
Size: 310 146 1146 5057
Fragments arranged by size:
5057 1146 310 146
EcoICRI GAG'CTC
Cuts at: 395 3671 395
Size: 3276 3383
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 332 804 1433 1457 1728 1775 1873 2049
Size: 199 472 629 24 271 47 98 176
Cuts at: 2049 2521 2720 2774 2834 2837 2918 3035 3551
Size: 472 199 54 60 3 81 117 516
Cuts at: 3551 4484 4916 5663 5931 133
Size: 933 432 747 268 861
Fragments arranged by size:
933 861 747 629 516 472 472 432
271 268 199 199 176 117 98 81
60 54 47 24 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1582 1582
Size: 6659
EcoO109I rG'GnC_Cy
Cuts at: 194 213 1694 2730 3369 3798 194
Size: 19 1481 1036 639 429 3055
Fragments arranged by size:
3055 1481 1036 639 429 19
EcoRI G'AATT_C
Cuts at: 1090 3663 1090
Size: 2573 4086
EcoRV GAT'ATC
Cuts at: 1612 1612
Size: 6659
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 423 2076 4678 423
Size: 1653 2602 2404
Fragments arranged by size:
2602 2404 1653
FspI TGC'GCA
Cuts at: 4440 4440
Size: 6659
HaeII r_GCGC'y
Cuts at: 4093 4341 5363 6342 4093
Size: 248 1022 979 4410
Fragments arranged by size:
4410 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1681 1713 2194 2226 3379 3411 3451 3483 4501
Size: 32 481 32 1153 32 40 32 1018
Cuts at: 4501 4531 4533 4563 1681
Size: 30 2 30 3777
Fragments arranged by size:
3777 1153 1018 481 40 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3698 3698
Size: 6659
HindIII A'AGCT_T
Cuts at: 329 1718 329
Size: 1389 5270
KasI G'GCGC_C
Cuts at: 4089 4337 4089
Size: 248 6411
KpnI G_GTAC'C
Cuts at: 630 3679 630
Size: 3049 3610
MluI A'CGCG_T
Cuts at: 6092 6092
Size: 6659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5330 5514 5330
Size: 184 6475
MscI TGG'CCA
Cuts at: 1276 3283 4420 1276
Size: 2007 1137 3515
Fragments arranged by size:
3515 2007 1137
MslI CAynn'nnrTG
Cuts at: 598 900 2201 2253 4648 4775 598
Size: 302 1301 52 2395 127 2482
Fragments arranged by size:
2482 2395 1301 302 127 52
MspA1I CmG'CkG
Cuts at: 167 278 655 726 2541 2764 2938 3334 3432
Size: 111 377 71 1815 223 174 396 98
Cuts at: 3432 3453 3574 3770 4084 4444 5457 5702 167
Size: 21 121 196 314 360 1013 245 1124
Fragments arranged by size:
1815 1124 1013 396 377 360 314 245
223 196 174 121 111 98 71 21
NaeI GCC'GGC
Cuts at: 4840 4840
Size: 6659
NarI GG'CG_CC
Cuts at: 4090 4338 4090
Size: 248 6411
NcoI C'CATG_G
Cuts at: 107 630 1076 107
Size: 523 446 5690
Fragments arranged by size:
5690 523 446
NgoMIV G'CCGG_C
Cuts at: 4838 4838
Size: 6659
NspI r_CATG'y
Cuts at: 1200 1670 2036 2200 2604 3712 4212 5119 1200
Size: 470 366 164 404 1108 500 907 2740
Fragments arranged by size:
2740 1108 907 500 470 404 366 164
PasI CC'CwG_GG
Cuts at: 718 718
Size: 6659
PciI A'CATG_T
Cuts at: 5115 5115
Size: 6659
PflMI CCAn_nnn'nTGG
Cuts at: 2827 2827
Size: 6659
PmeI GTTT'AAAC
Cuts at: 3658 3658
Size: 6659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5798 5830 5798
Size: 32 6627
PpuMI rG'GwC_Cy
Cuts at: 213 2730 213
Size: 2517 4142
PshAI GACnn'nnGTC
Cuts at: 639 639
Size: 6659
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 954 986 954
Size: 32 6627
PstI C_TGCA'G
Cuts at: 786 1110 1257 3089 3141 3551 3706 4391 786
Size: 324 147 1832 52 410 155 685 3054
Fragments arranged by size:
3054 1832 685 410 324 155 147 52
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6659
PvuII CAG'CTG
Cuts at: 278 655 726 2541 2764 2938 3432 3453 3574
Size: 377 71 1815 223 174 494 21 121
Cuts at: 3574 4084 4444 278
Size: 510 360 2493
Fragments arranged by size:
2493 1815 510 494 377 360 223 174
121 71 21
RsrII CG'GwC_CG
Cuts at: 4854 4854
Size: 6659
SacI G_AGCT'C
Cuts at: 397 3673 397
Size: 3276 3383
SacII CC_GC'GG
Cuts at: 168 168
Size: 6659
SalI G'TCGA_C
Cuts at: 3696 3696
Size: 6659
SanDI GG'GwC_CC
Cuts at: 213 213
Size: 6659
SapI GCTCTTCn'nnn_
Cuts at: 2690 3113 4682 4892 2690
Size: 423 1569 210 4457
Fragments arranged by size:
4457 1569 423 210
SbfI CC_TGCA'GG
Cuts at: 3551 3706 3551
Size: 155 6504
ScaI AGT'ACT
Cuts at: 1303 6176 1303
Size: 4873 1786
SfcI C'TryA_G
Cuts at: 33 765 782 914 1106 1253 2191 3085 3113
Size: 732 17 132 192 147 938 894 28
Cuts at: 3113 3137 3547 3556 3702 4387 5380 5571 33
Size: 24 410 9 146 685 993 191 1121
Fragments arranged by size:
1121 993 938 894 732 685 410 192
191 147 146 132 28 24 17 9
SfoI GGC'GCC
Cuts at: 4091 4339 4091
Size: 248 6411
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6659
SmaI CCC'GGG
Cuts at: 3588 3681 3588
Size: 93 6566
SmlI C'TyrA_G
Cuts at: 1395 3239 3814 4729 5221 5483 1395
Size: 1844 575 915 492 262 2571
Fragments arranged by size:
2571 1844 915 575 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6659
SphI G_CATG'C
Cuts at: 1670 2200 3712 4212 1670
Size: 530 1512 500 4117
Fragments arranged by size:
4117 1512 530 500
StuI AGG'CCT
Cuts at: 1954 1954
Size: 6659
StyI C'CwwG_G
Cuts at: 107 398 516 630 827 1076 2443 2771 2858
Size: 291 118 114 197 249 1367 328 87
Cuts at: 2858 3793 4147 107
Size: 935 354 2619
Fragments arranged by size:
2619 1367 935 354 328 291 249 197
118 114 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 271 564 912 2016 2265 2327 2931 3041 4242
Size: 293 348 1104 249 62 604 110 1201
Cuts at: 4242 4375 4834 5043 271
Size: 133 459 209 1887
Fragments arranged by size:
1887 1201 1104 604 459 348 293 249
209 133 110 62
TatI w'GTAC_w
Cuts at: 840 1159 1301 3624 6174 840
Size: 319 142 2323 2550 1325
Fragments arranged by size:
2550 2323 1325 319 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 835 867 2670 2702 6176 6208 835
Size: 32 1803 32 3474 32 1286
Fragments arranged by size:
3474 1803 1286 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2553 4456 2553
Size: 1903 4756
XbaI T'CTAG_A
Cuts at: 3690 3690
Size: 6659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2010 2538 3429 4154 2010
Size: 528 891 725 4515
Fragments arranged by size:
4515 891 725 528
XmaI C'CCGG_G
Cuts at: 3586 3679 3586
Size: 93 6566
XmnI GAAnn'nnTTC
Cuts at: 1318 2977 1318
Size: 1659 5000
ZraI GAC'GTC
Cuts at: 1509 1509
Size: 6659
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AlfI AloI ApaI AscI AvrII BbsI BfrBI
BmtI BsiWI BsmBI BssHII BstEII BstZ17I ClaI CspCI
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PsiI PspOMI PspXI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI