Restriction map for
pF1KE3718
(Circular) (Six-base) MAPSORT of: pF1KE3718.seq Check: 9932 from: 1 to: 10526
pF1KE3718 10526 bp
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 706 1171 1321 1891 5794 7096 706
Size: 465 150 570 3903 1302 4136
Fragments arranged by size:
4136 3903 1302 570 465 150
AccI GT'mk_AC
Cuts at: 3951 5696 7564 3951
Size: 1745 1868 6913
Fragments arranged by size:
6913 1868 1745
Acc65I G'GTAC_C
Cuts at: 1045 3241 4741 4816 7542 1045
Size: 2196 1500 75 2726 4029
Fragments arranged by size:
4029 2726 2196 1500 75
AclI AA'CG_TT
Cuts at: 10366 10366
Size: 10526
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1462 2799 5264 5441 6409 6995 8351 8783 9530
Size: 1337 2465 177 968 586 1356 432 747
Cuts at: 9530 9798 1462
Size: 268 2190
Fragments arranged by size:
2465 2190 1356 1337 968 747 586 432
268 177
AflIII A'CryG_T
Cuts at: 1543 2194 2792 3109 3223 6581 7481 7878 8982
Size: 651 598 317 114 3358 900 397 1104
Cuts at: 8982 9959 1543
Size: 977 2110
Fragments arranged by size:
3358 2110 1104 977 900 651 598 397
317 114
AgeI A'CCGG_T
Cuts at: 8914 8914
Size: 10526
AhdI GACnn_n'nnGTC
Cuts at: 4230 7380 4230
Size: 3150 7376
AleI CACnn'nnGTG
Cuts at: 1798 3476 1798
Size: 1678 8848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1043 4819 7298 7532 1043
Size: 3776 2479 234 4037
Fragments arranged by size:
4037 3776 2479 234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4766 4798 4766
Size: 32 10494
AlwNI CAG_nnn'CTG
Cuts at: 1662 3294 5892 6064 7243 7831 9398 1662
Size: 1632 2598 172 1179 588 1567 2790
Fragments arranged by size:
2790 2598 1632 1567 1179 588 172
ApaI G_GGCC'C
Cuts at: 5399 6368 7111 5399
Size: 969 743 8814
Fragments arranged by size:
8814 969 743
ApaLI G'TGCA_C
Cuts at: 498 782 3078 4180 9296 498
Size: 284 2296 1102 5116 1728
Fragments arranged by size:
5116 2296 1728 1102 284
ApoI r'AATT_y
Cuts at: 2664 7530 9879 10135 2664
Size: 4866 2349 256 3055
Fragments arranged by size:
4866 3055 2349 256
AseI AT'TA_AT
Cuts at: 20 2039 2096 20
Size: 2019 57 8450
Fragments arranged by size:
8450 2019 57
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10526
AvaI C'yCGr_G
Cuts at: 1897 2233 3758 3797 4110 4220 4326 4409 6161
Size: 336 1525 39 313 110 106 83 1752
Cuts at: 6161 6245 6257 6998 7546 1897
Size: 84 12 741 548 4877
Fragments arranged by size:
4877 1752 1525 741 548 336 313 110
106 84 83 39 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2052 2085 2433 2466 2721 2754 3326 3359 4733
Size: 33 348 33 255 33 572 33 1374
Cuts at: 4733 4766 2052
Size: 33 7812
Fragments arranged by size:
7812 1374 572 348 255 33 33 33
33 33
BamHI G'GATC_C
Cuts at: 3163 7551 3163
Size: 4388 6138
BanI G'GyrC_C
Cuts at: 694 808 1045 1420 1909 3241 3391 3706 4130
Size: 114 237 375 489 1332 150 315 424
Cuts at: 4130 4353 4741 4816 5704 6325 6937 7542 7956
Size: 223 388 75 888 621 612 605 414
Cuts at: 7956 8204 8239 694
Size: 248 35 2981
Fragments arranged by size:
2981 1332 888 621 612 605 489 424
414 388 375 315 248 237 223 150
114 75 35
BanII G_rGCy'C
Cuts at: 3091 5399 6368 7007 7071 7111 7540 8570 3091
Size: 2308 969 639 64 40 429 1030 5047
Fragments arranged by size:
5047 2308 1030 969 639 429 64 40
BbeI G_GCGC'C
Cuts at: 1424 1913 7960 8208 1424
Size: 489 6047 248 3742
Fragments arranged by size:
6047 3742 489 248
BbsI GAAGACnn'nnnn_
Cuts at: 5874 6232 5874
Size: 358 10168
BbvCI CC'TCA_GC
Cuts at: 4280 6382 6932 4280
Size: 2102 550 7874
Fragments arranged by size:
7874 2102 550
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 3547 3581 4548 4582 90
Size: 34 3423 34 967 34 6034
Fragments arranged by size:
6034 3423 967 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 247 480 2080 2434 3610 8424 9185 10526 247
Size: 233 1600 354 1176 4814 761 1341 247
Fragments arranged by size:
4814 1600 1341 1176 761 354 247 233
BclI T'GATC_A
Cuts at: 5996 8046 5996
Size: 2050 8476
BfrBI ATG'CAT
Cuts at: 181 1240 1244 181
Size: 1059 4 9463
Fragments arranged by size:
9463 1059 4
BglI GCCn_nnn'nGGC
Cuts at: 458 623 740 1322 2012 3266 3524 3752 5588
Size: 165 117 582 690 1254 258 228 1836
Cuts at: 5588 5968 6323 6472 458
Size: 380 355 149 4512
Fragments arranged by size:
4512 1836 1254 690 582 380 355 258
228 165 149 117
BglII A'GATC_T
Cuts at: 6373 8041 10078 6373
Size: 1668 2037 6821
Fragments arranged by size:
6821 2037 1668
BlpI GC'TnA_GC
Cuts at: 478 7301 7638 478
Size: 6823 337 3366
Fragments arranged by size:
6823 3366 337
Bme1580I G_kGCm'C
Cuts at: 502 736 779 786 1201 1272 3082 3148 3410
Size: 234 43 7 415 71 1810 66 262
Cuts at: 3410 3520 3798 4184 4317 4812 5399 6368 6940
Size: 110 278 386 133 495 587 969 572
Cuts at: 6940 7111 8151 8244 9300 502
Size: 171 1040 93 1056 1728
Fragments arranged by size:
1810 1728 1056 1040 969 587 572 495
415 386 278 262 234 171 133 110
93 71 66 43 7
BmgBI CAC'GTC
Cuts at: 2018 3405 2018
Size: 1387 9139
BmrI ACTGGGnnnn_n'
Cuts at: 1691 2942 3284 3357 3542 4208 4822 5388 6063
Size: 1251 342 73 185 666 614 566 675
Cuts at: 6063 6419 6676 6844 6888 7148 7307 7738 7819
Size: 356 257 168 44 260 159 431 81
Cuts at: 7819 8154 10298 1691
Size: 335 2144 1919
Fragments arranged by size:
2144 1919 1251 675 666 614 566 431
356 342 335 260 257 185 168 159
81 73 44
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2184 2757 3930 8893 2184
Size: 573 1173 4963 3817
Fragments arranged by size:
4963 3817 1173 573
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1116 1136 2333 3420 4768 5108 5698 6305 6788
Size: 20 1197 1087 1348 340 590 607 483
Cuts at: 6788 6911 7079 7315 7472 1116
Size: 123 168 236 157 4170
Fragments arranged by size:
4170 1348 1197 1087 607 590 483 340
236 168 157 123 20
Bpu10I CC'TnA_GC
Cuts at: 2281 4280 6382 6932 2281
Size: 1999 2102 550 5875
Fragments arranged by size:
5875 2102 1999 550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6849 7702 8581 9073 9371 6849
Size: 853 879 492 298 8004
Fragments arranged by size:
8004 879 853 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 296 1222 2323 2770 3320 6883 10230 296
Size: 926 1101 447 550 3563 3347 592
Fragments arranged by size:
3563 3347 1101 926 592 550 447
BsaAI yAC'GTr
Cuts at: 374 6584 7879 374
Size: 6210 1295 3021
Fragments arranged by size:
6210 3021 1295
BsaBI GATnn'nnATC
Cuts at: 6 1474 8040 10077 6
Size: 1468 6566 2037 455
Fragments arranged by size:
6566 2037 1468 455
BsaHI Gr'CG_yC
Cuts at: 1421 1910 4418 7957 8205 8907 10403 1421
Size: 489 2508 3539 248 702 1496 1544
Fragments arranged by size:
3539 2508 1544 1496 702 489 248
BsaWI w'CCGG_w
Cuts at: 5729 7715 8236 8914 9188 9335 9746 5729
Size: 1986 521 678 274 147 411 6509
Fragments arranged by size:
6509 1986 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 589 619 861 891 3528 3558 4492 4522 4766
Size: 30 242 30 2637 30 934 30 244
Cuts at: 4766 4796 6591 6621 7077 7107 589
Size: 30 1795 30 456 30 4008
Fragments arranged by size:
4008 2637 1795 934 456 244 242 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1825 2848 3562 4497 4696 5131 5525 5636 6566
Size: 1023 714 935 199 435 394 111 930
Cuts at: 6566 7469 1825
Size: 903 4882
Fragments arranged by size:
4882 1023 935 930 903 714 435 394
199 111
BseYI C'CCAG_C
Cuts at: 360 2251 2351 3877 4360 4523 5190 5399 6692
Size: 1891 100 1526 483 163 667 209 1293
Cuts at: 6692 6855 7073 7184 7218 7236 7951 8709 9286
Size: 163 218 111 34 18 715 758 577
Cuts at: 9286 9782 360
Size: 496 1104
Fragments arranged by size:
1891 1526 1293 1104 758 715 667 577
496 483 218 209 163 163 111 100
34 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 975 1326 4655 5577 5734 5828 975
Size: 351 3329 922 157 94 5673
Fragments arranged by size:
5673 3329 922 351 157 94
BsiEI CG_ry'CG
Cuts at: 104 1173 4568 8068 8114 9322 104
Size: 1069 3395 3500 46 1208 1308
Fragments arranged by size:
3500 3395 1308 1208 1069 46
BsiHKAI G_wGCw'C
Cuts at: 502 786 874 2694 3082 3091 3742 4184 7540
Size: 284 88 1820 388 9 651 442 3356
Cuts at: 7540 8318 8508 9300 502
Size: 778 190 792 1728
Fragments arranged by size:
3356 1820 1728 792 778 651 442 388
284 190 88 9
BsmI GAATG_Cn'
Cuts at: 1015 1777 2281 2392 2643 3196 4597 6024 1015
Size: 762 504 111 251 553 1401 1427 5517
Fragments arranged by size:
5517 1427 1401 762 553 504 251 111
BsmBI CGTCTCn'nnnn_
Cuts at: 5797 5797
Size: 10526
Bsp1286I G_dGCh'C
Cuts at: 502 736 779 786 874 1201 1272 2694 3082
Size: 234 43 7 88 327 71 1422 388
Cuts at: 3082 3091 3148 3410 3520 3742 3798 4184 4317
Size: 9 57 262 110 222 56 386 133
Cuts at: 4317 4812 5399 6368 6940 7007 7071 7111 7540
Size: 495 587 969 572 67 64 40 429
Cuts at: 7540 8151 8244 8318 8508 8570 9300 502
Size: 611 93 74 190 62 730 1728
Fragments arranged by size:
1728 1422 969 730 611 587 572 495
429 388 386 327 262 234 222 190
133 110 93 88 74 71 67 64
62 57 56 43 40 9 7
BspEI T'CCGG_A
Cuts at: 9746 9746
Size: 10526
BspHI T'CATG_A
Cuts at: 6720 9702 6720
Size: 2982 7544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 706 716 1171 1204 1321 1891 2542 3004 4844
Size: 10 455 33 117 570 651 462 1840
Cuts at: 4844 5794 7096 7188 7500 7576 7790 8092 8473
Size: 950 1302 92 312 76 214 302 381
Cuts at: 8473 706
Size: 2759
Fragments arranged by size:
2759 1840 1302 950 651 570 462 455
381 312 302 214 117 92 76 33
10
BsrBI CCG'CTC
Cuts at: 50 127 8818 8872 10356 10521 50
Size: 77 8691 54 1484 165 55
Fragments arranged by size:
8691 1484 165 77 55 54
BsrDI GCAATG_nn'
Cuts at: 181 1068 3807 8438 181
Size: 887 2739 4631 2269
Fragments arranged by size:
4631 2739 2269 887
BsrFI r'CCGG_y
Cuts at: 4001 8524 8705 8914 4001
Size: 4523 181 209 5613
Fragments arranged by size:
5613 4523 209 181
BssSI C'ACGA_G
Cuts at: 8797 9155 8797
Size: 358 10168
BstAPI GCAn_nnn'nTGC
Cuts at: 183 1256 1952 2291 4179 4613 5869 6481 7903
Size: 1073 696 339 1888 434 1256 612 1422
Cuts at: 7903 183
Size: 2806
Fragments arranged by size:
2806 1888 1422 1256 1073 696 612 434
339
BstBI TT'CG_AA
Cuts at: 2395 8887 2395
Size: 6492 4034
BstEII G'GTnAC_C
Cuts at: 7324 7324
Size: 10526
BstXI CCAn_nnnn'nTGG
Cuts at: 334 691 3383 4459 6863 334
Size: 357 2692 1076 2404 3997
Fragments arranged by size:
3997 2692 2404 1076 357
BstYI r'GATC_y
Cuts at: 1 3163 4930 6188 6373 6629 7551 8018 8041
Size: 3162 1767 1258 185 256 922 467 23
Cuts at: 8041 8376 8622 9634 9720 9732 10078 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
3162 1767 1258 1012 922 467 449 346
335 256 246 185 86 23 12
BstZ17I GTA'TAC
Cuts at: 3952 3952
Size: 10526
Bsu36I CC'TnA_GG
Cuts at: 387 737 5537 387
Size: 350 4800 5376
Fragments arranged by size:
5376 4800 350
BtgI C'CryG_G
Cuts at: 1367 1848 2073 2298 2760 4481 5470 5577 6033
Size: 481 225 225 462 1721 989 107 456
Cuts at: 6033 1367
Size: 5860
Fragments arranged by size:
5860 1721 989 481 462 456 225 225
107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5501 8476 8657 8820 5501
Size: 2975 181 163 7207
Fragments arranged by size:
7207 2975 181 163
BtsI GCAGTG_nn'
Cuts at: 193 678 1119 1636 1926 3606 4180 5019 5864
Size: 485 441 517 290 1680 574 839 845
Cuts at: 5864 6029 6662 6816 6822 7169 7769 193
Size: 165 633 154 6 347 600 2950
Fragments arranged by size:
2950 1680 845 839 633 600 574 517
485 441 347 290 165 154 6
ClaI AT'CG_AT
Cuts at: 1751 1751
Size: 10526
DraI TTT'AAA
Cuts at: 2469 7525 2469
Size: 5056 5470
DraIII CAC_nnn'GTG
Cuts at: 796 1276 2571 6798 796
Size: 480 1295 4227 4524
Fragments arranged by size:
4524 4227 1295 480
DrdI GACnn_nn'nnGTC
Cuts at: 7482 8232 9090 7482
Size: 750 858 8918
Fragments arranged by size:
8918 858 750
EaeI y'GGCC_r
Cuts at: 593 1370 3999 4070 5467 6659 8111 8285 8676
Size: 777 2629 71 1397 1192 1452 174 391
Cuts at: 8676 8703 10048 593
Size: 27 1345 1071
Fragments arranged by size:
2629 1452 1397 1345 1192 1071 777 391
174 71 27
EagI C'GGCC_G
Cuts at: 8111 8111
Size: 10526
EarI CTCTTCn'nnn_
Cuts at: 3770 5261 5673 6053 6131 8549 8759 3770
Size: 1491 412 380 78 2418 210 5537
Fragments arranged by size:
5537 2418 1491 412 380 210 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 8734 9044 9190 10336 8734
Size: 310 146 1146 8924
Fragments arranged by size:
8924 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3089 7538 3089
Size: 4449 6077
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1116 1136 1462 2333 2799 3420 4768 5108 5264
Size: 20 326 871 466 621 1348 340 156
Cuts at: 5264 5441 5698 6305 6409 6788 6911 6995 7079
Size: 177 257 607 104 379 123 84 84
Cuts at: 7079 7315 7472 8351 8783 9530 9798 1116
Size: 236 157 879 432 747 268 1844
Fragments arranged by size:
1844 1348 879 871 747 621 607 466
432 379 340 326 268 257 236 177
157 156 123 104 84 84 20
EcoNI CCTnn'n_nnAGG
Cuts at: 879 3214 6466 879
Size: 2335 3252 4939
Fragments arranged by size:
4939 3252 2335
EcoO109I rG'GnC_Cy
Cuts at: 1456 2239 2993 3762 3802 5078 5395 5396 7108
Size: 783 754 769 40 1276 317 1 1712
Cuts at: 7108 7468 7665 1456
Size: 360 197 4317
Fragments arranged by size:
4317 1712 1276 783 769 754 360 317
197 40 1
EcoRI G'AATT_C
Cuts at: 7530 7530
Size: 10526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2081 2481 8545 2081
Size: 400 6064 4062
Fragments arranged by size:
6064 4062 400
FspI TGC'GCA
Cuts at: 4840 8307 4840
Size: 3467 7059
HaeII r_GCGC'y
Cuts at: 142 151 1424 1913 4287 7960 8208 9230 10209
Size: 9 1273 489 2374 3673 248 1022 979
Cuts at: 10209 142
Size: 459
Fragments arranged by size:
3673 2374 1273 1022 979 489 459 248
9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3553 3585 4216 4248 6327 6359 6365 6397 6586
Size: 32 631 32 2079 32 6 32 189
Cuts at: 6586 6618 7366 7398 8368 8398 8400 8430 3553
Size: 32 748 32 970 30 2 30 5649
Fragments arranged by size:
5649 2079 970 748 631 189 32 32
32 32 32 32 30 30 6 2
HincII GTy'rAC
Cuts at: 295 945 1089 3012 3654 6335 7565 295
Size: 650 144 1923 642 2681 1230 3256
Fragments arranged by size:
3256 2681 1923 1230 650 642 144
HindIII A'AGCT_T
Cuts at: 6825 6825
Size: 10526
KasI G'GCGC_C
Cuts at: 1420 1909 7956 8204 1420
Size: 489 6047 248 3742
Fragments arranged by size:
6047 3742 489 248
KpnI G_GTAC'C
Cuts at: 1049 3245 4745 4820 7546 1049
Size: 2196 1500 75 2726 4029
Fragments arranged by size:
4029 2726 2196 1500 75
MluI A'CGCG_T
Cuts at: 9959 9959
Size: 10526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3596 3941 6549 6617 9197 9381 3596
Size: 345 2608 68 2580 184 4741
Fragments arranged by size:
4741 2608 2580 345 184 68
MscI TGG'CCA
Cuts at: 595 1372 4072 6661 8287 595
Size: 777 2700 2589 1626 2834
Fragments arranged by size:
2834 2700 2589 1626 777
MslI CAynn'nnrTG
Cuts at: 186 563 1232 1548 1760 1798 3381 3476 6400
Size: 377 669 316 212 38 1583 95 2924
Cuts at: 6400 6584 6880 7174 7280 8515 8642 186
Size: 184 296 294 106 1235 127 2070
Fragments arranged by size:
2924 2070 1583 1235 669 377 316 296
294 212 184 127 106 95 38
MspA1I CmG'CkG
Cuts at: 323 962 2222 2255 2712 3251 3257 4142 4202
Size: 639 1260 33 457 539 6 885 60
Cuts at: 4202 4271 4337 4900 5840 5866 6061 6931 7063
Size: 69 66 563 940 26 195 870 132
Cuts at: 7063 7099 7464 7637 7951 8311 9324 9569 323
Size: 36 365 173 314 360 1013 245 1280
Fragments arranged by size:
1280 1260 1013 940 885 870 639 563
539 457 365 360 314 245 195 173
132 69 66 60 36 33 26 6
NaeI GCC'GGC
Cuts at: 4003 8707 4003
Size: 4704 5822
NarI GG'CG_CC
Cuts at: 1421 1910 7957 8205 1421
Size: 489 6047 248 3742
Fragments arranged by size:
6047 3742 489 248
NcoI C'CATG_G
Cuts at: 1367 1848 2073 2298 2760 4481 5470 6033 1367
Size: 481 225 225 462 1721 989 563 5860
Fragments arranged by size:
5860 1721 989 563 481 462 225 225
NdeI CA'TA_TG
Cuts at: 473 6230 473
Size: 5757 4769
NgoMIV G'CCGG_C
Cuts at: 4001 8705 4001
Size: 4704 5822
NsiI A_TGCA'T
Cuts at: 183 1242 1246 183
Size: 1059 4 9463
Fragments arranged by size:
9463 1059 4
NspI r_CATG'y
Cuts at: 181 470 1197 1244 1396 1547 1557 2198 2796
Size: 289 727 47 152 151 10 641 598
Cuts at: 2796 3113 3599 4083 4616 6123 6270 6700 7485
Size: 317 486 484 533 1507 147 430 785
Cuts at: 7485 7509 7579 8079 8986 181
Size: 24 70 500 907 1721
Fragments arranged by size:
1721 1507 907 785 727 641 598 533
500 486 484 430 317 289 152 151
147 70 47 24 10
PasI CC'CwG_GG
Cuts at: 2781 3264 3522 4493 4857 6625 7441 2781
Size: 483 258 971 364 1768 816 5866
Fragments arranged by size:
5866 1768 971 816 483 364 258
PciI A'CATG_T
Cuts at: 1543 2194 2792 3109 7481 8982 1543
Size: 651 598 317 4372 1501 3087
Fragments arranged by size:
4372 3087 1501 651 598 317
PflMI CCAn_nnn'nTGG
Cuts at: 6811 6811
Size: 10526
PfoI T'CCnGG_A
Cuts at: 672 3436 4784 5795 672
Size: 2764 1348 1011 5403
Fragments arranged by size:
5403 2764 1348 1011
PmeI GTTT'AAAC
Cuts at: 7525 7525
Size: 10526
PmlI CAC'GTG
Cuts at: 374 374
Size: 10526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4766 4798 9665 9697 4766
Size: 32 4867 32 5595
Fragments arranged by size:
5595 4867 32 32
PpuMI rG'GwC_Cy
Cuts at: 1456 2239 2993 3802 5078 7468 1456
Size: 783 754 809 1276 2390 4514
Fragments arranged by size:
4514 2390 1276 809 783 754
PshAI GACnn'nnGTC
Cuts at: 652 652
Size: 10526
PspOMI G'GGCC_C
Cuts at: 5395 6364 7107 5395
Size: 969 743 8814
Fragments arranged by size:
8814 969 743
PstI C_TGCA'G
Cuts at: 1022 1114 1921 3256 3310 3928 5499 5859 7573
Size: 92 807 1335 54 618 1571 360 1714
Cuts at: 7573 8258 1022
Size: 685 3290
Fragments arranged by size:
3290 1714 1571 1335 807 685 618 360
92 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10526
PvuII CAG'CTG
Cuts at: 2255 3257 4142 4271 4900 5866 6061 6931 7099
Size: 1002 885 129 629 966 195 870 168
Cuts at: 7099 7951 8311 2255
Size: 852 360 4470
Fragments arranged by size:
4470 1002 966 885 870 852 629 360
195 168 129
RsrII CG'GwC_CG
Cuts at: 8721 8721
Size: 10526
SacI G_AGCT'C
Cuts at: 3091 7540 3091
Size: 4449 6077
SalI G'TCGA_C
Cuts at: 7563 7563
Size: 10526
SanDI GG'GwC_CC
Cuts at: 2239 3802 5078 2239
Size: 1563 1276 7687
Fragments arranged by size:
7687 1563 1276
SapI GCTCTTCn'nnn_
Cuts at: 6053 8549 8759 6053
Size: 2496 210 7820
Fragments arranged by size:
7820 2496 210
SbfI CC_TGCA'GG
Cuts at: 7573 7573
Size: 10526
ScaI AGT'ACT
Cuts at: 415 757 1722 10043 415
Size: 342 965 8321 898
Fragments arranged by size:
8321 965 898 342
SexAI A'CCwGG_T
Cuts at: 1979 1979
Size: 10526
SfcI C'TryA_G
Cuts at: 33 1018 1110 1917 2103 3252 3306 3849 3924
Size: 985 92 807 186 1149 54 543 75
Cuts at: 3924 5495 5855 7569 8254 9247 9438 33
Size: 1571 360 1714 685 993 191 1121
Fragments arranged by size:
1714 1571 1149 1121 993 985 807 685
543 360 191 186 92 75 54
SfoI GGC'GCC
Cuts at: 1422 1911 7958 8206 1422
Size: 489 6047 248 3742
Fragments arranged by size:
6047 3742 489 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10526
SmaI CCC'GGG
Cuts at: 1899 3799 4112 4222 6163 6247 7548 1899
Size: 1900 313 110 1941 84 1301 4877
Fragments arranged by size:
4877 1941 1900 1301 313 110 84
SmlI C'TyrA_G
Cuts at: 6828 7681 8596 9088 9350 6828
Size: 853 915 492 262 8004
Fragments arranged by size:
8004 915 853 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10526
SphI G_CATG'C
Cuts at: 181 1244 1396 1557 6270 6700 7579 8079 181
Size: 1063 152 161 4713 430 879 500 2628
Fragments arranged by size:
4713 2628 1063 879 500 430 161 152
SspI AAT'ATT
Cuts at: 289 888 2493 289
Size: 599 1605 8322
Fragments arranged by size:
8322 1605 599
StuI AGG'CCT
Cuts at: 4432 5431 6640 4432
Size: 999 1209 8318
Fragments arranged by size:
8318 1209 999
StyI C'CwwG_G
Cuts at: 1367 1848 2073 2298 2526 2760 4481 5470 5874
Size: 481 225 225 228 234 1721 989 404
Cuts at: 5874 6033 6467 6485 7660 8014 1367
Size: 159 434 18 1175 354 3879
Fragments arranged by size:
3879 1721 1175 989 481 434 404 354
234 228 225 225 159 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2794 3302 6660 6955 7326 8109 8242 8701 8910
Size: 508 3358 295 371 783 133 459 209
Cuts at: 8910 2794
Size: 4410
Fragments arranged by size:
4410 3358 783 508 459 371 295 209
133
TatI w'GTAC_w
Cuts at: 413 755 1147 1720 2308 2449 6896 10041 413
Size: 342 392 573 588 141 4447 3145 898
Fragments arranged by size:
4447 3145 898 588 573 392 342 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 861 893 1391 1423 3528 3560 5936 5968 6591
Size: 32 498 32 2105 32 2376 32 623
Cuts at: 6591 6623 6744 6776 7077 7109 10043 10075 861
Size: 32 121 32 301 32 2934 32 1312
Fragments arranged by size:
2934 2376 2105 1312 623 498 301 121
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1981 3007 5082 5692 8323 1981
Size: 1026 2075 610 2631 4184
Fragments arranged by size:
4184 2631 2075 1026 610
XbaI T'CTAG_A
Cuts at: 5916 6415 7557 5916
Size: 499 1142 8885
Fragments arranged by size:
8885 1142 499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3814 4141 4520 5793 6654 8021 3814
Size: 327 379 1273 861 1367 6319
Fragments arranged by size:
6319 1367 1273 861 379 327
XmaI C'CCGG_G
Cuts at: 1897 3797 4110 4220 6161 6245 7546 1897
Size: 1900 313 110 1941 84 1301 4877
Fragments arranged by size:
4877 1941 1900 1301 313 110 84
XmnI GAAnn'nnTTC
Cuts at: 2215 2902 3730 2215
Size: 687 828 9011
Fragments arranged by size:
9011 828 687
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AscI AvrII BmtI BsiWI BsrGI
BssHII CspCI EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI PacI PsiI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI