Restriction map for
pF1KE3662
(Circular) (Six-base) MAPSORT of: pF1KE3662.seq Check: 7577 from: 1 to: 6041
pF1KE3662 6041 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1210 1210
Size: 6041
AccI GT'mk_AC
Cuts at: 695 1190 2869 3079 695
Size: 495 1679 210 3657
Fragments arranged by size:
3657 1679 495 210
Acc65I G'GTAC_C
Cuts at: 3057 3057
Size: 6041
AclI AA'CG_TT
Cuts at: 5881 5881
Size: 6041
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1873 1882 2023 3866 4298 5045 5313 1873
Size: 9 141 1843 432 747 268 2601
Fragments arranged by size:
2601 1843 747 432 268 141 9
AfeI AGC'GCT
Cuts at: 610 610
Size: 6041
AflIII A'CryG_T
Cuts at: 302 2504 3393 4497 5474 302
Size: 2202 889 1104 977 869
Fragments arranged by size:
2202 1104 977 889 869
AgeI A'CCGG_T
Cuts at: 4429 4429
Size: 6041
AhdI GACnn_n'nnGTC
Cuts at: 1068 1068
Size: 6041
AleI CACnn'nnGTG
Cuts at: 796 1117 1294 796
Size: 321 177 5543
Fragments arranged by size:
5543 321 177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1684 2696 1684
Size: 1012 5029
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2221 2253 2221
Size: 32 6009
AlwNI CAG_nnn'CTG
Cuts at: 449 729 888 1635 1686 3346 4913 449
Size: 280 159 747 51 1660 1567 1577
Fragments arranged by size:
1660 1577 1567 747 280 159 51
ApaI G_GGCC'C
Cuts at: 2205 2205
Size: 6041
ApaLI G'TGCA_C
Cuts at: 1669 2314 4811 1669
Size: 645 2497 2899
Fragments arranged by size:
2899 2497 645
ApoI r'AATT_y
Cuts at: 1675 3045 5394 5650 1675
Size: 1370 2349 256 2066
Fragments arranged by size:
2349 2066 1370 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6041
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6041
AvaI C'yCGr_G
Cuts at: 712 1219 2013 2410 3061 712
Size: 507 794 397 651 3692
Fragments arranged by size:
3692 794 651 507 397
BamHI G'GATC_C
Cuts at: 3026 3066 3026
Size: 40 6001
BanI G'GyrC_C
Cuts at: 439 499 664 790 813 870 1015 1053 1129
Size: 60 165 126 23 57 145 38 76
Cuts at: 1129 2028 2817 3057 3471 3719 3754 439
Size: 899 789 240 414 248 35 2726
Fragments arranged by size:
2726 899 789 414 248 240 165 145
126 76 60 57 38 35 23
BanII G_rGCy'C
Cuts at: 116 230 401 836 973 1319 1424 2205 3055
Size: 114 171 435 137 346 105 781 850
Cuts at: 3055 4085 116
Size: 1030 2072
Fragments arranged by size:
2072 1030 850 781 435 346 171 137
114 105
BbeI G_GCGC'C
Cuts at: 443 3475 3723 443
Size: 3032 248 2761
Fragments arranged by size:
3032 2761 248
BbsI GAAGACnn'nnnn_
Cuts at: 1870 2011 1870
Size: 141 5900
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 555 589 2325 2359 555
Size: 34 1736 34 4237
Fragments arranged by size:
4237 1736 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3939 4700 6041 3939
Size: 761 1341 3939
Fragments arranged by size:
3939 1341 761
BclI T'GATC_A
Cuts at: 2174 3561 2174
Size: 1387 4654
BglI GCCn_nnn'nGGC
Cuts at: 175 673 784 879 899 928 2144 2467 175
Size: 498 111 95 20 29 1216 323 3749
Fragments arranged by size:
3749 1216 498 323 111 95 29 20
BglII A'GATC_T
Cuts at: 3556 5593 3556
Size: 2037 4004
BlpI GC'TnA_GC
Cuts at: 380 1462 3153 380
Size: 1082 1691 3268
Fragments arranged by size:
3268 1691 1082
Bme1580I G_kGCm'C
Cuts at: 712 875 988 1056 1673 1809 2205 2318 2685
Size: 163 113 68 617 136 396 113 367
Cuts at: 2685 2739 2822 3666 3759 4815 712
Size: 54 83 844 93 1056 1938
Fragments arranged by size:
1938 1056 844 617 396 367 163 136
113 113 93 83 68 54
BmgBI CAC'GTC
Cuts at: 2505 2505
Size: 6041
BmrI ACTGGGnnnn_n'
Cuts at: 369 2571 3253 3334 3669 5813 369
Size: 2202 682 81 335 2144 597
Fragments arranged by size:
2202 2144 682 597 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 244 627 1593 4408 244
Size: 383 966 2815 1877
Fragments arranged by size:
2815 1877 966 383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 100 239 1333 1459 1494 2419 2866 100
Size: 139 1094 126 35 925 447 3275
Fragments arranged by size:
3275 1094 925 447 139 126 35
Bpu10I CC'TnA_GC
Cuts at: 989 989
Size: 6041
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 2734 2986 3217 4096 4588 4886 385
Size: 2349 252 231 879 492 298 1540
Fragments arranged by size:
2349 1540 879 492 298 252 231
BsaI GGTCTCn'nnnn_
Cuts at: 739 5745 739
Size: 5006 1035
BsaAI yAC'GTr
Cuts at: 415 588 1872 3394 415
Size: 173 1284 1522 3062
Fragments arranged by size:
3062 1522 1284 173
BsaBI GATnn'nnATC
Cuts at: 6 2176 2446 3031 3555 5592 6
Size: 2170 270 585 524 2037 455
Fragments arranged by size:
2170 2037 585 524 455 270
BsaHI Gr'CG_yC
Cuts at: 440 1153 1358 2147 3472 3720 4422 5918 440
Size: 713 205 789 1325 248 702 1496 563
Fragments arranged by size:
1496 1325 789 713 702 563 248 205
BsaWI w'CCGG_w
Cuts at: 2480 3230 3751 4429 4703 4850 5261 2480
Size: 750 521 678 274 147 411 3260
Fragments arranged by size:
3260 750 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 292 322 2620 2650 292
Size: 30 2298 30 3683
Fragments arranged by size:
3683 2298 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 386 619 1262 386
Size: 233 643 5165
Fragments arranged by size:
5165 643 233
BseYI C'CCAG_C
Cuts at: 1065 2794 2994 3466 4224 4801 5297 1065
Size: 1729 200 472 758 577 496 1809
Fragments arranged by size:
1809 1729 758 577 496 472 200
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 1648 2938 869
Size: 779 1290 3972
Fragments arranged by size:
3972 1290 779
BsiEI CG_ry'CG
Cuts at: 104 170 2891 3583 3629 4837 104
Size: 66 2721 692 46 1208 1308
Fragments arranged by size:
2721 1308 1208 692 66 46
BsiHKAI G_wGCw'C
Cuts at: 116 230 401 995 1673 2318 2879 3055 3833
Size: 114 171 594 678 645 561 176 778
Cuts at: 3833 4023 4815 116
Size: 190 792 1342
Fragments arranged by size:
1342 792 778 678 645 594 561 190
176 171 114
BsmI GAATG_Cn'
Cuts at: 1237 1237
Size: 6041
BsmBI CGTCTCn'nnnn_
Cuts at: 1365 1473 1365
Size: 108 5933
Bsp1286I G_dGCh'C
Cuts at: 116 230 401 712 836 875 973 988 995
Size: 114 171 311 124 39 98 15 7
Cuts at: 995 1056 1319 1424 1673 1809 2205 2318 2685
Size: 61 263 105 249 136 396 113 367
Cuts at: 2685 2739 2822 2879 3055 3666 3759 3833 4023
Size: 54 83 57 176 611 93 74 190
Cuts at: 4023 4085 4815 116
Size: 62 730 1342
Fragments arranged by size:
1342 730 611 396 367 311 263 249
190 176 171 136 124 114 113 105
98 93 83 74 62 61 57 54
39 15 7
BspEI T'CCGG_A
Cuts at: 5261 5261
Size: 6041
BspHI T'CATG_A
Cuts at: 281 2654 5217 281
Size: 2373 2563 1105
Fragments arranged by size:
2563 2373 1105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1210 3091 3305 3607 3988 1210
Size: 1881 214 302 381 3263
Fragments arranged by size:
3263 1881 381 302 214
BsrBI CCG'CTC
Cuts at: 50 624 2553 2804 4333 4387 5871 6036 50
Size: 574 1929 251 1529 54 1484 165 55
Fragments arranged by size:
1929 1529 1484 574 251 165 55 54
BsrDI GCAATG_nn'
Cuts at: 539 1359 3953 539
Size: 820 2594 2627
Fragments arranged by size:
2627 2594 820
BsrFI r'CCGG_y
Cuts at: 754 787 1657 2031 2056 2098 2890 4039 4220
Size: 33 870 374 25 42 792 1149 181
Cuts at: 4220 4429 754
Size: 209 2366
Fragments arranged by size:
2366 1149 870 792 374 209 181 42
33 25
BsrGI T'GTAC_A
Cuts at: 1988 1988
Size: 6041
BssSI C'ACGA_G
Cuts at: 2578 2799 4312 4670 2578
Size: 221 1513 358 3949
Fragments arranged by size:
3949 1513 358 221
BstAPI GCAn_nnn'nTGC
Cuts at: 999 3418 999
Size: 2419 3622
BstBI TT'CG_AA
Cuts at: 4402 4402
Size: 6041
BstEII G'GTnAC_C
Cuts at: 1371 1371
Size: 6041
BstXI CCAn_nnnn'nTGG
Cuts at: 1617 2625 1617
Size: 1008 5033
BstYI r'GATC_y
Cuts at: 1 1699 1928 2441 3026 3066 3533 3556 3891
Size: 1698 229 513 585 40 467 23 335
Cuts at: 3891 4137 5149 5235 5247 5593 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1698 1012 585 513 467 449 346 335
246 229 86 40 23 12
BstZ17I GTA'TAC
Cuts at: 2870 2870
Size: 6041
Bsu36I CC'TnA_GG
Cuts at: 1010 1058 1160 1010
Size: 48 102 5891
Fragments arranged by size:
5891 102 48
BtgI C'CryG_G
Cuts at: 107 164 170 614 809 1118 1340 2624 107
Size: 57 6 444 195 309 222 1284 3524
Fragments arranged by size:
3524 1284 444 309 222 195 57 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 735 2259 2964 3991 4172 4335 735
Size: 1524 705 1027 181 163 2441
Fragments arranged by size:
2441 1524 1027 705 181 163
BtsI GCAGTG_nn'
Cuts at: 857 889 2668 2897 3284 857
Size: 32 1779 229 387 3614
Fragments arranged by size:
3614 1779 387 229 32
DraI TTT'AAA
Cuts at: 3040 3040
Size: 6041
DraIII CAC_nnn'GTG
Cuts at: 2583 2583
Size: 6041
DrdI GACnn_nn'nnGTC
Cuts at: 3747 4605 3747
Size: 858 5183
EaeI y'GGCC_r
Cuts at: 161 167 776 785 2036 2297 2888 2964 3626
Size: 6 609 9 1251 261 591 76 662
Cuts at: 3626 3800 4191 4218 5563 161
Size: 174 391 27 1345 639
Fragments arranged by size:
1345 1251 662 639 609 591 391 261
174 76 27 9 6
EagI C'GGCC_G
Cuts at: 167 2888 3626 167
Size: 2721 738 2582
Fragments arranged by size:
2721 2582 738
EarI CTCTTCn'nnn_
Cuts at: 824 4064 4274 824
Size: 3240 210 2591
Fragments arranged by size:
3240 2591 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1086 2125 2628 4249 4559 4705 5851 1086
Size: 1039 503 1621 310 146 1146 1276
Fragments arranged by size:
1621 1276 1146 1039 503 310 146
EcoICRI GAG'CTC
Cuts at: 114 228 399 3053 114
Size: 114 171 2654 3102
Fragments arranged by size:
3102 2654 171 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 100 239 1333 1459 1494 1873 1882 2023 2419
Size: 139 1094 126 35 379 9 141 396
Cuts at: 2419 2866 3866 4298 5045 5313 100
Size: 447 1000 432 747 268 828
Fragments arranged by size:
1094 1000 828 747 447 432 396 379
268 141 139 126 35 9
EcoNI CCTnn'n_nnAGG
Cuts at: 892 1621 2092 892
Size: 729 471 4841
Fragments arranged by size:
4841 729 471
EcoO109I rG'GnC_Cy
Cuts at: 264 726 1062 1893 2201 2202 3180 264
Size: 462 336 831 308 1 978 3125
Fragments arranged by size:
3125 978 831 462 336 308 1
EcoRI G'AATT_C
Cuts at: 1675 3045 1675
Size: 1370 4671
EcoRV GAT'ATC
Cuts at: 660 660
Size: 6041
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4060 4060
Size: 6041
FspI TGC'GCA
Cuts at: 3822 3822
Size: 6041
HaeII r_GCGC'y
Cuts at: 443 519 612 679 3475 3723 4745 5724 443
Size: 76 93 67 2796 248 1022 979 760
Fragments arranged by size:
2796 1022 979 760 248 93 76 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1054 1086 1143 1175 2620 2652 3883 3913 3915
Size: 32 57 32 1445 32 1231 30 2
Cuts at: 3915 3945 1054
Size: 30 3150
Fragments arranged by size:
3150 1445 1231 57 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2349 3080 2349
Size: 731 5310
KasI G'GCGC_C
Cuts at: 439 3471 3719 439
Size: 3032 248 2761
Fragments arranged by size:
3032 2761 248
KpnI G_GTAC'C
Cuts at: 3061 3061
Size: 6041
MluI A'CGCG_T
Cuts at: 5474 5474
Size: 6041
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 464 1568 2597 4712 4896 464
Size: 1104 1029 2115 184 1609
Fragments arranged by size:
2115 1609 1104 1029 184
MscI TGG'CCA
Cuts at: 778 2038 2299 3802 778
Size: 1260 261 1503 3017
Fragments arranged by size:
3017 1503 1260 261
MslI CAynn'nnrTG
Cuts at: 248 796 1117 1294 1886 2524 2623 2857 4030
Size: 548 321 177 592 638 99 234 1173
Cuts at: 4030 4157 248
Size: 127 2132
Fragments arranged by size:
2132 1173 638 592 548 321 234 177
127 99
MspA1I CmG'CkG
Cuts at: 166 172 217 1169 2253 2281 2453 3152 3466
Size: 6 45 952 1084 28 172 699 314
Cuts at: 3466 3826 4839 5084 166
Size: 360 1013 245 1123
Fragments arranged by size:
1123 1084 1013 952 699 360 314 245
172 45 28 6
NaeI GCC'GGC
Cuts at: 756 789 1659 2058 2100 2892 4222 756
Size: 33 870 399 42 792 1330 2575
Fragments arranged by size:
2575 1330 870 792 399 42 33
NarI GG'CG_CC
Cuts at: 440 3472 3720 440
Size: 3032 248 2761
Fragments arranged by size:
3032 2761 248
NcoI C'CATG_G
Cuts at: 107 2624 107
Size: 2517 3524
NdeI CA'TA_TG
Cuts at: 1887 1887
Size: 6041
NgoMIV G'CCGG_C
Cuts at: 754 787 1657 2056 2098 2890 4220 754
Size: 33 870 399 42 792 1330 2575
Fragments arranged by size:
2575 1330 870 792 399 42 33
NotI GC'GGCC_GC
Cuts at: 167 167
Size: 6041
NspI r_CATG'y
Cuts at: 294 306 2001 2265 3094 3594 4501 294
Size: 12 1695 264 829 500 907 1834
Fragments arranged by size:
1834 1695 907 829 500 264 12
PciI A'CATG_T
Cuts at: 302 4497 302
Size: 4195 1846
PflMI CCAn_nnn'nTGG
Cuts at: 810 2453 810
Size: 1643 4398
PfoI T'CCnGG_A
Cuts at: 653 1471 2717 653
Size: 818 1246 3977
Fragments arranged by size:
3977 1246 818
PmeI GTTT'AAAC
Cuts at: 3040 3040
Size: 6041
PmlI CAC'GTG
Cuts at: 588 588
Size: 6041
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5180 5212 5180
Size: 32 6009
PpuMI rG'GwC_Cy
Cuts at: 264 1062 1893 264
Size: 798 831 4412
Fragments arranged by size:
4412 831 798
PspOMI G'GGCC_C
Cuts at: 2201 2201
Size: 6041
PstI C_TGCA'G
Cuts at: 216 725 2252 3088 3773 216
Size: 509 1527 836 685 2484
Fragments arranged by size:
2484 1527 836 685 509
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6041
PvuII CAG'CTG
Cuts at: 217 2253 2281 3466 3826 217
Size: 2036 28 1185 360 2432
Fragments arranged by size:
2432 2036 1185 360 28
RsrII CG'GwC_CG
Cuts at: 4236 4236
Size: 6041
SacI G_AGCT'C
Cuts at: 116 230 401 3055 116
Size: 114 171 2654 3102
Fragments arranged by size:
3102 2654 171 114
SacII CC_GC'GG
Cuts at: 167 173 167
Size: 6 6035
SalI G'TCGA_C
Cuts at: 3078 3078
Size: 6041
SapI GCTCTTCn'nnn_
Cuts at: 824 4064 4274 824
Size: 3240 210 2591
Fragments arranged by size:
3240 2591 210
SbfI CC_TGCA'GG
Cuts at: 3088 3088
Size: 6041
ScaI AGT'ACT
Cuts at: 5558 5558
Size: 6041
SexAI A'CCwGG_T
Cuts at: 1616 2357 3008 1616
Size: 741 651 4649
Fragments arranged by size:
4649 741 651
SfcI C'TryA_G
Cuts at: 33 212 721 1191 2248 2985 3084 3769 4762
Size: 179 509 470 1057 737 99 685 993
Cuts at: 4762 4953 33
Size: 191 1121
Fragments arranged by size:
1121 1057 993 737 685 509 470 191
179 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 784 784
Size: 6041
SfoI GGC'GCC
Cuts at: 441 3473 3721 441
Size: 3032 248 2761
Fragments arranged by size:
3032 2761 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6041
SmaI CCC'GGG
Cuts at: 3063 3063
Size: 6041
SmlI C'TyrA_G
Cuts at: 400 2749 3001 3196 4111 4603 4865 400
Size: 2349 252 195 915 492 262 1576
Fragments arranged by size:
2349 1576 915 492 262 252 195
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6041
SphI G_CATG'C
Cuts at: 2001 2265 3094 3594 2001
Size: 264 829 500 4448
Fragments arranged by size:
4448 829 500 264
StuI AGG'CCT
Cuts at: 931 1367 931
Size: 436 5605
StyI C'CwwG_G
Cuts at: 107 1133 1427 2624 3175 3529 107
Size: 1026 294 1197 551 354 2619
Fragments arranged by size:
2619 1197 1026 551 354 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 351 1117 2637 3624 3757 4216 4425 351
Size: 766 1520 987 133 459 209 1967
Fragments arranged by size:
1967 1520 987 766 459 209 133
TatI w'GTAC_w
Cuts at: 305 1988 2186 5556 305
Size: 1683 198 3370 790
Fragments arranged by size:
3370 1683 790 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 847 879 5558 5590 847
Size: 32 4679 32 1298
Fragments arranged by size:
4679 1298 32 32
Tth111I GACn'n_nGTC
Cuts at: 1186 1686 2707 3838 1186
Size: 500 1021 1131 3389
Fragments arranged by size:
3389 1131 1021 500
XbaI T'CTAG_A
Cuts at: 3072 3072
Size: 6041
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1434 2910 3536 1434
Size: 1476 626 3939
Fragments arranged by size:
3939 1476 626
XmaI C'CCGG_G
Cuts at: 3061 3061
Size: 6041
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NotI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AscI AvrII BaeI BbvCI BfrBI BmtI
BsiWI BssHII ClaI CspCI FseI FspAI HindIII HpaI
MfeI NheI NruI NsiI PacI PasI PshAI PsiI
PspXI PsrI SanDI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI