Restriction map for
pF1KE3483
(Circular) (Six-base) MAPSORT of: pF1KE3483.seq Check: 6971 from: 1 to: 8351
pF1KE3483 8351 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4111 4572 4111
Size: 461 7890
AccI GT'mk_AC
Cuts at: 5389 5389
Size: 8351
Acc65I G'GTAC_C
Cuts at: 5367 5367
Size: 8351
AclI AA'CG_TT
Cuts at: 8191 8191
Size: 8351
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 915 2615 2861 3314 4071 4316 4391 4442
Size: 337 1700 246 453 757 245 75 51
Cuts at: 4442 4972 6176 6608 7355 7623 578
Size: 530 1204 432 747 268 1306
Fragments arranged by size:
1700 1306 1204 757 747 530 453 432
337 268 246 245 75 51
AfeI AGC'GCT
Cuts at: 2878 2878
Size: 8351
AflIII A'CryG_T
Cuts at: 135 5703 6807 7784 135
Size: 5568 1104 977 702
Fragments arranged by size:
5568 1104 977 702
AgeI A'CCGG_T
Cuts at: 6739 6739
Size: 8351
AhdI GACnn_n'nnGTC
Cuts at: 3744 4077 3744
Size: 333 8018
AleI CACnn'nnGTG
Cuts at: 754 754
Size: 8351
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1766 1798 1766
Size: 32 8319
AlwNI CAG_nnn'CTG
Cuts at: 205 2515 2821 4248 5028 5103 5656 7223 205
Size: 2310 306 1427 780 75 553 1567 1333
Fragments arranged by size:
2310 1567 1427 1333 780 553 306 75
ApaI G_GGCC'C
Cuts at: 1582 3887 5116 1582
Size: 2305 1229 4817
Fragments arranged by size:
4817 2305 1229
ApaLI G'TGCA_C
Cuts at: 7121 7121
Size: 8351
ApoI r'AATT_y
Cuts at: 1074 1888 5330 5355 7704 7960 1074
Size: 814 3442 25 2349 256 1465
Fragments arranged by size:
3442 2349 1465 814 256 25
AseI AT'TA_AT
Cuts at: 20 20
Size: 8351
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8351
AvaI C'yCGr_G
Cuts at: 1573 3281 4810 5012 5371 1573
Size: 1708 1529 202 359 4553
Fragments arranged by size:
4553 1708 1529 359 202
BamHI G'GATC_C
Cuts at: 3285 4326 5066 5376 3285
Size: 1041 740 310 6260
Fragments arranged by size:
6260 1041 740 310
BanI G'GyrC_C
Cuts at: 213 5367 5781 6029 6064 213
Size: 5154 414 248 35 2500
Fragments arranged by size:
5154 2500 414 248 35
BanII G_rGCy'C
Cuts at: 1262 1582 2209 3887 4730 5116 5365 6395 1262
Size: 320 627 1678 843 386 249 1030 3218
Fragments arranged by size:
3218 1678 1030 843 627 386 320 249
BbeI G_GCGC'C
Cuts at: 5785 6033 5785
Size: 248 8103
BbsI GAAGACnn'nnnn_
Cuts at: 2155 2252 3063 3389 4031 4382 2155
Size: 97 811 326 642 351 6124
Fragments arranged by size:
6124 811 642 351 326 97
BbvCI CC'TCA_GC
Cuts at: 3795 3795
Size: 8351
BciVI GTATCCnnnnn_n'
Cuts at: 579 6249 7010 8351 579
Size: 5670 761 1341 579
Fragments arranged by size:
5670 1341 761 579
BclI T'GATC_A
Cuts at: 3584 5216 5871 3584
Size: 1632 655 6064
Fragments arranged by size:
6064 1632 655
BfrBI ATG'CAT
Cuts at: 2008 3175 2008
Size: 1167 7184
BglI GCCn_nnn'nGGC
Cuts at: 2553 2975 2553
Size: 422 7929
BglII A'GATC_T
Cuts at: 4184 5124 5866 7903 4184
Size: 940 742 2037 4632
Fragments arranged by size:
4632 2037 940 742
BlpI GC'TnA_GC
Cuts at: 373 5463 373
Size: 5090 3261
Bme1580I G_kGCm'C
Cuts at: 1582 1671 3887 5116 5976 6069 7125 1582
Size: 89 2216 1229 860 93 1056 2808
Fragments arranged by size:
2808 2216 1229 1056 860 93 89
BmrI ACTGGGnnnn_n'
Cuts at: 3696 4857 5563 5644 5979 8123 3696
Size: 1161 706 81 335 2144 3924
Fragments arranged by size:
3924 2144 1161 706 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2336 3627 6718 2336
Size: 1291 3091 3969
Fragments arranged by size:
3969 3091 1291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 606 1613 2432 2492 2990 3855 5080 606
Size: 1007 819 60 498 865 1225 3877
Fragments arranged by size:
3877 1225 1007 865 819 498 60
Bpu10I CC'TnA_GC
Cuts at: 497 2873 3795 497
Size: 2376 922 5053
Fragments arranged by size:
5053 2376 922
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 2306 3080 3967 4639 5284 5527 6406 6898
Size: 1847 774 887 672 645 243 879 492
Cuts at: 6898 7196 459
Size: 298 1614
Fragments arranged by size:
1847 1614 887 879 774 672 645 492
298 243
BsaI GGTCTCn'nnnn_
Cuts at: 1089 1333 3777 8055 1089
Size: 244 2444 4278 1385
Fragments arranged by size:
4278 2444 1385 244
BsaAI yAC'GTr
Cuts at: 5704 5704
Size: 8351
BsaBI GATnn'nnATC
Cuts at: 6 5865 7902 6
Size: 5859 2037 455
Fragments arranged by size:
5859 2037 455
BsaHI Gr'CG_yC
Cuts at: 4379 5081 5782 6030 6732 8228 4379
Size: 702 701 248 702 1496 4502
Fragments arranged by size:
4502 1496 702 702 701 248
BsaWI w'CCGG_w
Cuts at: 5540 6061 6739 7013 7160 7571 5540
Size: 521 678 274 147 411 6320
Fragments arranged by size:
6320 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 933 963 1828 1858 2652 2682 2724 2754 933
Size: 30 865 30 794 30 42 30 6530
Fragments arranged by size:
6530 865 794 42 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 823 859 862 2435 2938 2990 2993 3216 3269
Size: 36 3 1573 503 52 3 223 53
Cuts at: 3269 4488 823
Size: 1219 4686
Fragments arranged by size:
4686 1573 1219 503 223 53 52 36
3 3
BseYI C'CCAG_C
Cuts at: 1438 2298 4039 4547 4729 5776 6534 7111 7607
Size: 860 1741 508 182 1047 758 577 496
Cuts at: 7607 1438
Size: 2182
Fragments arranged by size:
2182 1741 1047 860 758 577 508 496
182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3309 4900 3309
Size: 1591 6760
BsiEI CG_ry'CG
Cuts at: 104 5893 5939 7147 104
Size: 5789 46 1208 1308
Fragments arranged by size:
5789 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 751 854 1571 2533 4625 5283 5365 6143 6333
Size: 103 717 962 2092 658 82 778 190
Cuts at: 6333 7125 751
Size: 792 1977
Fragments arranged by size:
2092 1977 962 792 778 717 658 190
103 82
BsmI GAATG_Cn'
Cuts at: 160 1103 3177 160
Size: 943 2074 5334
Fragments arranged by size:
5334 2074 943
Bsp1286I G_dGCh'C
Cuts at: 751 854 1262 1571 1582 1671 2209 2533 3887
Size: 103 408 309 11 89 538 324 1354
Cuts at: 3887 4625 4730 5116 5283 5365 5976 6069 6143
Size: 738 105 386 167 82 611 93 74
Cuts at: 6143 6333 6395 7125 751
Size: 190 62 730 1977
Fragments arranged by size:
1977 1354 738 730 611 538 408 386
324 309 190 167 105 103 93 89
82 74 62 11
BspEI T'CCGG_A
Cuts at: 7571 7571
Size: 8351
BspHI T'CATG_A
Cuts at: 1938 4457 5213 7527 1938
Size: 2519 756 2314 2762
Fragments arranged by size:
2762 2519 2314 756
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2743 4111 4572 4679 4988 5401 5615 5917 6298
Size: 1368 461 107 309 413 214 302 381
Cuts at: 6298 2743
Size: 4796
Fragments arranged by size:
4796 1368 461 413 381 309 302 214
107
BsrBI CCG'CTC
Cuts at: 50 5000 5064 6643 6697 8181 8346 50
Size: 4950 64 1579 54 1484 165 55
Fragments arranged by size:
4950 1579 1484 165 64 55 54
BsrDI GCAATG_nn'
Cuts at: 237 6263 237
Size: 6026 2325
BsrFI r'CCGG_y
Cuts at: 776 3875 6349 6530 6739 776
Size: 3099 2474 181 209 2388
Fragments arranged by size:
3099 2474 2388 209 181
BsrGI T'GTAC_A
Cuts at: 1395 3077 1395
Size: 1682 6669
BssSI C'ACGA_G
Cuts at: 853 4970 6622 6980 853
Size: 4117 1652 358 2224
Fragments arranged by size:
4117 2224 1652 358
BstAPI GCAn_nnn'nTGC
Cuts at: 164 1357 2863 3172 3323 3544 4126 5728 164
Size: 1193 1506 309 151 221 582 1602 2787
Fragments arranged by size:
2787 1602 1506 1193 582 309 221 151
BstBI TT'CG_AA
Cuts at: 6712 6712
Size: 8351
BstEII G'GTnAC_C
Cuts at: 1222 2755 1222
Size: 1533 6818
BstXI CCAn_nnnn'nTGG
Cuts at: 3858 4714 3858
Size: 856 7495
BstYI r'GATC_y
Cuts at: 1 2306 3285 3393 4158 4184 4326 4814 5066
Size: 2305 979 108 765 26 142 488 252
Cuts at: 5066 5124 5376 5843 5866 6201 6447 7459 7545
Size: 58 252 467 23 335 246 1012 86
Cuts at: 7545 7557 7903 1
Size: 12 346 449
Fragments arranged by size:
2305 1012 979 765 488 467 449 346
335 252 252 246 142 108 86 58
26 23 12
BtgI C'CryG_G
Cuts at: 225 890 1556 3632 225
Size: 665 666 2076 4944
Fragments arranged by size:
4944 2076 666 665
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3492 6301 6482 6645 3492
Size: 2809 181 163 5198
Fragments arranged by size:
5198 2809 181 163
BtsI GCAGTG_nn'
Cuts at: 2487 3568 3705 4647 5206 5594 2487
Size: 1081 137 942 559 388 5244
Fragments arranged by size:
5244 1081 942 559 388 137
DraI TTT'AAA
Cuts at: 171 2892 5350 171
Size: 2721 2458 3172
Fragments arranged by size:
3172 2721 2458
DraIII CAC_nnn'GTG
Cuts at: 4485 4485
Size: 8351
DrdI GACnn_nn'nnGTC
Cuts at: 6057 6915 6057
Size: 858 7493
EaeI y'GGCC_r
Cuts at: 887 1559 2333 5030 5936 6110 6501 6528 7873
Size: 672 774 2697 906 174 391 27 1345
Cuts at: 7873 887
Size: 1365
Fragments arranged by size:
2697 1365 1345 906 774 672 391 174
27
EagI C'GGCC_G
Cuts at: 5936 5936
Size: 8351
EarI CTCTTCn'nnn_
Cuts at: 812 872 1367 1469 2483 3193 4358 4786 5134
Size: 60 495 102 1014 710 1165 428 348
Cuts at: 5134 6374 6584 812
Size: 1240 210 2579
Fragments arranged by size:
2579 1240 1165 1014 710 495 428 348
210 102 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2893 6559 6869 7015 8161 2893
Size: 3666 310 146 1146 3083
Fragments arranged by size:
3666 3083 1146 310 146
EcoICRI GAG'CTC
Cuts at: 5363 5363
Size: 8351
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 606 915 1613 2432 2492 2615 2861 2990
Size: 28 309 698 819 60 123 246 129
Cuts at: 2990 3314 3855 4071 4316 4391 4442 4972 5080
Size: 324 541 216 245 75 51 530 108
Cuts at: 5080 6176 6608 7355 7623 578
Size: 1096 432 747 268 1306
Fragments arranged by size:
1306 1096 819 747 698 541 530 432
324 309 268 246 245 216 129 123
108 75 60 51 28
EcoNI CCTnn'n_nnAGG
Cuts at: 3095 3762 3095
Size: 667 7684
EcoO109I rG'GnC_Cy
Cuts at: 1578 1579 3618 3884 5112 5113 5490 1578
Size: 1 2039 266 1228 1 377 4439
Fragments arranged by size:
4439 2039 1228 377 266 1 1
EcoRI G'AATT_C
Cuts at: 5355 5355
Size: 8351
EcoRV GAT'ATC
Cuts at: 2885 3675 2885
Size: 790 7561
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 183 1372 2114 4180 6370 183
Size: 1189 742 2066 2190 2164
Fragments arranged by size:
2190 2164 2066 1189 742
FspI TGC'GCA
Cuts at: 3775 6132 3775
Size: 2357 5994
HaeII r_GCGC'y
Cuts at: 2880 3018 5785 6033 7055 8034 2880
Size: 138 2767 248 1022 979 3197
Fragments arranged by size:
3197 2767 1022 979 248 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1117 1149 3228 3260 3730 3762 4063 4095 6193
Size: 32 2079 32 470 32 301 32 2098
Cuts at: 6193 6223 6225 6255 1117
Size: 30 2 30 3213
Fragments arranged by size:
3213 2098 2079 470 301 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1587 2813 5390 1587
Size: 1226 2577 4548
Fragments arranged by size:
4548 2577 1226
HindIII A'AGCT_T
Cuts at: 679 2474 2895 679
Size: 1795 421 6135
Fragments arranged by size:
6135 1795 421
HpaI GTT'AAC
Cuts at: 1587 1587
Size: 8351
KasI G'GCGC_C
Cuts at: 5781 6029 5781
Size: 248 8103
KpnI G_GTAC'C
Cuts at: 5371 5371
Size: 8351
MluI A'CGCG_T
Cuts at: 7784 7784
Size: 8351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2454 2890 3875 7022 7206 2454
Size: 436 985 3147 184 3599
Fragments arranged by size:
3599 3147 985 436 184
MscI TGG'CCA
Cuts at: 889 1561 2335 5032 6112 889
Size: 672 774 2697 1080 3128
Fragments arranged by size:
3128 2697 1080 774 672
MslI CAynn'nnrTG
Cuts at: 230 754 2099 2989 3992 6340 6467 230
Size: 524 1345 890 1003 2348 127 2114
Fragments arranged by size:
2348 2114 1345 1003 890 524 127
MspA1I CmG'CkG
Cuts at: 2860 4076 5028 5036 5462 5776 6136 7149 7394
Size: 1216 952 8 426 314 360 1013 245
Cuts at: 7394 2860
Size: 3817
Fragments arranged by size:
3817 1216 1013 952 426 360 314 245
8
NaeI GCC'GGC
Cuts at: 3877 6532 3877
Size: 2655 5696
NarI GG'CG_CC
Cuts at: 5782 6030 5782
Size: 248 8103
NcoI C'CATG_G
Cuts at: 225 890 1556 3632 225
Size: 665 666 2076 4944
Fragments arranged by size:
4944 2076 666 665
NdeI CA'TA_TG
Cuts at: 2010 4149 2010
Size: 2139 6212
NgoMIV G'CCGG_C
Cuts at: 3875 6530 3875
Size: 2655 5696
NsiI A_TGCA'T
Cuts at: 2010 3177 2010
Size: 1167 7184
NspI r_CATG'y
Cuts at: 139 733 2994 5404 5904 6811 139
Size: 594 2261 2410 500 907 1679
Fragments arranged by size:
2410 2261 1679 907 594 500
PasI CC'CwG_GG
Cuts at: 4319 4319
Size: 8351
PciI A'CATG_T
Cuts at: 135 6807 135
Size: 6672 1679
PflMI CCAn_nnn'nTGG
Cuts at: 4485 4803 4485
Size: 318 8033
PfoI T'CCnGG_A
Cuts at: 4820 4820
Size: 8351
PmeI GTTT'AAAC
Cuts at: 5350 5350
Size: 8351
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7490 7522 7490
Size: 32 8319
PpuMI rG'GwC_Cy
Cuts at: 3618 3618
Size: 8351
PshAI GACnn'nnGTC
Cuts at: 4280 4280
Size: 8351
PsiI TTA'TAA
Cuts at: 1901 1901
Size: 8351
PspOMI G'GGCC_C
Cuts at: 1578 3883 5112 1578
Size: 2305 1229 4817
Fragments arranged by size:
4817 2305 1229
PspXI vC'TCGA_Gb
Cuts at: 1573 1573
Size: 8351
PstI C_TGCA'G
Cuts at: 1013 4725 5024 5339 5398 6083 1013
Size: 3712 299 315 59 685 3281
Fragments arranged by size:
3712 3281 685 315 299 59
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8351
PvuII CAG'CTG
Cuts at: 2860 4076 5028 5036 5776 6136 2860
Size: 1216 952 8 740 360 5075
Fragments arranged by size:
5075 1216 952 740 360 8
RsrII CG'GwC_CG
Cuts at: 6546 6546
Size: 8351
SacI G_AGCT'C
Cuts at: 5365 5365
Size: 8351
SalI G'TCGA_C
Cuts at: 5388 5388
Size: 8351
SapI GCTCTTCn'nnn_
Cuts at: 872 1469 2483 4786 6374 6584 872
Size: 597 1014 2303 1588 210 2639
Fragments arranged by size:
2639 2303 1588 1014 597 210
SbfI CC_TGCA'GG
Cuts at: 5398 5398
Size: 8351
ScaI AGT'ACT
Cuts at: 7868 7868
Size: 8351
SexAI A'CCwGG_T
Cuts at: 147 2806 147
Size: 2659 5692
SfcI C'TryA_G
Cuts at: 33 317 1009 1427 2277 3181 4721 5020 5303
Size: 284 692 418 850 904 1540 299 283
Cuts at: 5303 5335 5394 6079 7072 7263 33
Size: 32 59 685 993 191 1121
Fragments arranged by size:
1540 1121 993 904 850 692 685 418
299 284 283 191 59 32
SfoI GGC'GCC
Cuts at: 5783 6031 5783
Size: 248 8103
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8351
SmaI CCC'GGG
Cuts at: 5014 5373 5014
Size: 359 7992
SmlI C'TyrA_G
Cuts at: 438 1573 2321 3095 3946 4618 5263 5506 6421
Size: 1135 748 774 851 672 645 243 915
Cuts at: 6421 6913 7175 438
Size: 492 262 1614
Fragments arranged by size:
1614 1135 915 851 774 748 672 645
492 262 243
SpeI A'CTAG_T
Cuts at: 67 1762 67
Size: 1695 6656
SphI G_CATG'C
Cuts at: 5404 5904 5404
Size: 500 7851
StuI AGG'CCT
Cuts at: 3768 3768
Size: 8351
StyI C'CwwG_G
Cuts at: 225 890 1556 1789 3485 3614 3632 3758 4707
Size: 665 666 233 1696 129 18 126 949
Cuts at: 4707 5485 5839 225
Size: 778 354 2737
Fragments arranged by size:
2737 1696 949 778 666 665 354 233
129 126 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 231 238 1839 3555 5934 6067 6526 6735 231
Size: 7 1601 1716 2379 133 459 209 1847
Fragments arranged by size:
2379 1847 1716 1601 459 209 133 7
TatI w'GTAC_w
Cuts at: 825 1395 2631 3077 5160 7866 825
Size: 570 1236 446 2083 2706 1310
Fragments arranged by size:
2706 2083 1310 1236 570 446
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 135 167 933 965 2601 2633 2652 2684 7868
Size: 32 766 32 1636 32 19 32 5184
Cuts at: 7868 7900 135
Size: 32 586
Fragments arranged by size:
5184 1636 766 586 32 32 32 32
32 19
Tth111I GACn'n_nGTC
Cuts at: 6148 6148
Size: 8351
XbaI T'CTAG_A
Cuts at: 3445 5382 3445
Size: 1937 6414
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 339 3492 4060 4690 5846 339
Size: 3153 568 630 1156 2844
Fragments arranged by size:
3153 2844 1156 630 568
XhoI C'TCGA_G
Cuts at: 1573 1573
Size: 8351
XmaI C'CCGG_G
Cuts at: 5012 5371 5012
Size: 359 7992
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AflII AlfI AscI AvrII BaeI BcgI BmgBI
BmtI BsiWI BsmBI BssHII BstZ17I Bsu36I ClaI CspCI
FseI FspAI MfeI NheI NotI NruI PacI PmlI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI