Restriction map for
pF1KE3478
(Circular) (Six-base) MAPSORT of: pF1KE3478.seq Check: 3217 from: 1 to: 7952
pF1KE3478 7952 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 865 2243 4277 4990 865
Size: 1378 2034 713 3827
Fragments arranged by size:
3827 2034 1378 713
Acc65I G'GTAC_C
Cuts at: 4968 4968
Size: 7952
AclI AA'CG_TT
Cuts at: 7792 7792
Size: 7952
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 912 2904 3319 4217 5777 6209 6956 7224 912
Size: 1992 415 898 1560 432 747 268 1640
Fragments arranged by size:
1992 1640 1560 898 747 432 415 268
AflII C'TTAA_G
Cuts at: 3408 3408
Size: 7952
AflIII A'CryG_T
Cuts at: 1484 2199 3850 5304 6408 7385 1484
Size: 715 1651 1454 1104 977 2051
Fragments arranged by size:
2051 1651 1454 1104 977 715
AgeI A'CCGG_T
Cuts at: 6340 6340
Size: 7952
AleI CACnn'nnGTG
Cuts at: 3725 3725
Size: 7952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 965 3743 965
Size: 2778 5174
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 7920
AlwNI CAG_nnn'CTG
Cuts at: 146 448 1372 3141 4366 4804 5257 6824 146
Size: 302 924 1769 1225 438 453 1567 1274
Fragments arranged by size:
1769 1567 1274 1225 924 453 438 302
ApaLI G'TGCA_C
Cuts at: 2189 6722 2189
Size: 4533 3419
ApoI r'AATT_y
Cuts at: 343 647 1508 1700 2039 2132 2209 4609 4956
Size: 304 861 192 339 93 77 2400 347
Cuts at: 4956 7305 7561 343
Size: 2349 256 734
Fragments arranged by size:
2400 2349 861 734 347 339 304 256
192 93 77
AseI AT'TA_AT
Cuts at: 20 3221 20
Size: 3201 4751
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7952
AvaI C'yCGr_G
Cuts at: 1451 3284 4427 4972 1451
Size: 1833 1143 545 4431
Fragments arranged by size:
4431 1833 1143 545
BamHI G'GATC_C
Cuts at: 4977 4977
Size: 7952
BanI G'GyrC_C
Cuts at: 1084 4015 4968 5382 5630 5665 1084
Size: 2931 953 414 248 35 3371
Fragments arranged by size:
3371 2931 953 414 248 35
BanII G_rGCy'C
Cuts at: 2364 2576 2971 3124 4497 4966 5996 2364
Size: 212 395 153 1373 469 1030 4320
Fragments arranged by size:
4320 1373 1030 469 395 212 153
BbeI G_GCGC'C
Cuts at: 5386 5634 5386
Size: 248 7704
BbsI GAAGACnn'nnnn_
Cuts at: 1184 1700 1987 3533 3944 1184
Size: 516 287 1546 411 5192
Fragments arranged by size:
5192 1546 516 411 287
BbvCI CC'TCA_GC
Cuts at: 1886 3163 3860 1886
Size: 1277 697 5978
Fragments arranged by size:
5978 1277 697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2895 2929 2895
Size: 34 7918
BciVI GTATCCnnnnn_n'
Cuts at: 1134 3907 5850 6611 7952 1134
Size: 2773 1943 761 1341 1134
Fragments arranged by size:
2773 1943 1341 1134 761
BclI T'GATC_A
Cuts at: 1808 4092 4143 4442 5472 1808
Size: 2284 51 299 1030 4288
Fragments arranged by size:
4288 2284 1030 299 51
BglI GCCn_nnn'nGGC
Cuts at: 112 1955 112
Size: 1843 6109
BglII A'GATC_T
Cuts at: 1421 3471 5467 7504 1421
Size: 2050 1996 2037 1869
Fragments arranged by size:
2050 2037 1996 1869
BlpI GC'TnA_GC
Cuts at: 5064 5064
Size: 7952
Bme1580I G_kGCm'C
Cuts at: 2193 2804 4020 4811 5577 5670 6726 2193
Size: 611 1216 791 766 93 1056 3419
Fragments arranged by size:
3419 1216 1056 791 766 611 93
BmrI ACTGGGnnnn_n'
Cuts at: 585 1052 1350 1509 1632 1682 2277 3975 5164
Size: 467 298 159 123 50 595 1698 1189
Cuts at: 5164 5245 5580 7724 585
Size: 81 335 2144 813
Fragments arranged by size:
2144 1698 1189 813 595 467 335 298
159 123 81 50
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4916 6319 4916
Size: 1403 6549
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 327 852 992 1078 2676 327
Size: 525 140 86 1598 5603
Fragments arranged by size:
5603 1598 525 140 86
Bpu10I CC'TnA_GC
Cuts at: 1886 3163 3731 3860 1886
Size: 1277 568 129 5978
Fragments arranged by size:
5978 1277 568 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1143 2140 5128 6007 6499 6797 1143
Size: 997 2988 879 492 298 2298
Fragments arranged by size:
2988 2298 997 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3350 7656 3350
Size: 4306 3646
BsaAI yAC'GTr
Cuts at: 4309 5305 4309
Size: 996 6956
BsaBI GATnn'nnATC
Cuts at: 6 1208 2740 3470 4187 5466 7503 6
Size: 1202 1532 730 717 1279 2037 455
Fragments arranged by size:
2037 1532 1279 1202 730 717 455
BsaHI Gr'CG_yC
Cuts at: 5383 5631 6333 7829 5383
Size: 248 702 1496 5506
Fragments arranged by size:
5506 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3512 5141 5662 6340 6614 6761 7172 3512
Size: 1629 521 678 274 147 411 4292
Fragments arranged by size:
4292 1629 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3840 3870 3840
Size: 30 7922
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 138 3848 4864 4905 4932 138
Size: 3710 1016 41 27 3158
Fragments arranged by size:
3710 3158 1016 41 27
BseYI C'CCAG_C
Cuts at: 3444 3665 4578 5377 6135 6712 7208 3444
Size: 221 913 799 758 577 496 4188
Fragments arranged by size:
4188 913 799 758 577 496 221
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 1814 385
Size: 1429 6523
BsiEI CG_ry'CG
Cuts at: 104 5494 5540 6748 104
Size: 5390 46 1208 1308
Fragments arranged by size:
5390 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 155 838 2193 2364 2901 3124 3732 4966 5744
Size: 683 1355 171 537 223 608 1234 778
Cuts at: 5744 5934 6726 155
Size: 190 792 1381
Fragments arranged by size:
1381 1355 1234 792 778 683 608 537
223 190 171
BsmI GAATG_Cn'
Cuts at: 237 237
Size: 7952
BsmBI CGTCTCn'nnnn_
Cuts at: 1119 1671 1119
Size: 552 7400
Bsp1286I G_dGCh'C
Cuts at: 155 838 2193 2364 2576 2804 2901 2971 3124
Size: 683 1355 171 212 228 97 70 153
Cuts at: 3124 3732 4020 4497 4811 4966 5577 5670 5744
Size: 608 288 477 314 155 611 93 74
Cuts at: 5744 5934 5996 6726 155
Size: 190 62 730 1381
Fragments arranged by size:
1381 1355 730 683 611 608 477 314
288 228 212 190 171 155 153 97
93 74 70 62
BspEI T'CCGG_A
Cuts at: 7172 7172
Size: 7952
BspHI T'CATG_A
Cuts at: 7128 7128
Size: 7952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1081 1993 5002 5216 5518 5899 1081
Size: 912 3009 214 302 381 3134
Fragments arranged by size:
3134 3009 912 381 302 214
BsrBI CCG'CTC
Cuts at: 50 163 6244 6298 7782 7947 50
Size: 113 6081 54 1484 165 55
Fragments arranged by size:
6081 1484 165 113 55 54
BsrDI GCAATG_nn'
Cuts at: 1193 2444 2802 4035 5864 1193
Size: 1251 358 1233 1829 3281
Fragments arranged by size:
3281 1829 1251 1233 358
BsrFI r'CCGG_y
Cuts at: 4673 5950 6131 6340 4673
Size: 1277 181 209 6285
Fragments arranged by size:
6285 1277 209 181
BsrGI T'GTAC_A
Cuts at: 4933 4933
Size: 7952
BssSI C'ACGA_G
Cuts at: 6223 6581 6223
Size: 358 7594
BstAPI GCAn_nnn'nTGC
Cuts at: 5329 5329
Size: 7952
BstBI TT'CG_AA
Cuts at: 6313 6313
Size: 7952
BstXI CCAn_nnnn'nTGG
Cuts at: 1324 3807 1324
Size: 2483 5469
BstYI r'GATC_y
Cuts at: 1 484 543 1279 1421 1467 3471 4408 4977
Size: 483 59 736 142 46 2004 937 569
Cuts at: 4977 5444 5467 5802 6048 7060 7146 7158 7504
Size: 467 23 335 246 1012 86 12 346
Cuts at: 7504 1
Size: 449
Fragments arranged by size:
2004 1012 937 736 569 483 467 449
346 335 246 142 86 59 46 23
12
BstZ17I GTA'TAC
Cuts at: 866 4278 866
Size: 3412 4540
Bsu36I CC'TnA_GG
Cuts at: 121 1928 121
Size: 1807 6145
BtgI C'CryG_G
Cuts at: 107 911 1472 107
Size: 804 561 6587
Fragments arranged by size:
6587 804 561
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3932 5902 6083 6246 3932
Size: 1970 181 163 5638
Fragments arranged by size:
5638 1970 181 163
BtsI GCAGTG_nn'
Cuts at: 767 1301 2193 2802 3498 4009 4167 4479 5195
Size: 534 892 609 696 511 158 312 716
Cuts at: 5195 767
Size: 3524
Fragments arranged by size:
3524 892 716 696 609 534 511 312
158
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2673 2708 2673
Size: 35 7917
DraI TTT'AAA
Cuts at: 4951 4951
Size: 7952
DrdI GACnn_nn'nnGTC
Cuts at: 2746 5658 6516 2746
Size: 2912 858 4182
Fragments arranged by size:
4182 2912 858
EaeI y'GGCC_r
Cuts at: 655 1579 2694 5537 5711 6102 6129 7474 655
Size: 924 1115 2843 174 391 27 1345 1133
Fragments arranged by size:
2843 1345 1133 1115 924 391 174 27
EagI C'GGCC_G
Cuts at: 5537 5537
Size: 7952
EarI CTCTTCn'nnn_
Cuts at: 2923 4418 5975 6185 2923
Size: 1495 1557 210 4690
Fragments arranged by size:
4690 1557 1495 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6160 6470 6616 7762 6160
Size: 310 146 1146 6350
Fragments arranged by size:
6350 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2362 3122 4964 2362
Size: 760 1842 5350
Fragments arranged by size:
5350 1842 760
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 327 852 912 992 1078 2676 2904 3319 4217
Size: 525 60 80 86 1598 228 415 898
Cuts at: 4217 5777 6209 6956 7224 327
Size: 1560 432 747 268 1055
Fragments arranged by size:
1598 1560 1055 898 747 525 432 415
268 228 86 80 60
EcoNI CCTnn'n_nnAGG
Cuts at: 1217 2381 1217
Size: 1164 6788
EcoO109I rG'GnC_Cy
Cuts at: 1932 3390 5091 1932
Size: 1458 1701 4793
Fragments arranged by size:
4793 1701 1458
EcoRI G'AATT_C
Cuts at: 2209 4956 2209
Size: 2747 5205
EcoRV GAT'ATC
Cuts at: 2031 2031
Size: 7952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 793 3809 4541 5971 793
Size: 3016 732 1430 2774
Fragments arranged by size:
3016 2774 1430 732
FspI TGC'GCA
Cuts at: 5733 5733
Size: 7952
HaeII r_GCGC'y
Cuts at: 5386 5634 6656 7635 5386
Size: 248 1022 979 5703
Fragments arranged by size:
5703 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 889 921 1800 1832 2015 2047 3229 3261 3814
Size: 32 879 32 183 32 1182 32 553
Cuts at: 3814 3846 4287 4319 5794 5824 5826 5856 889
Size: 32 441 32 1475 30 2 30 2985
Fragments arranged by size:
2985 1475 1182 879 553 441 183 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1563 4991 1563
Size: 3428 4524
HindIII A'AGCT_T
Cuts at: 691 2096 691
Size: 1405 6547
HpaI GTT'AAC
Cuts at: 1563 1563
Size: 7952
KasI G'GCGC_C
Cuts at: 5382 5630 5382
Size: 248 7704
KpnI G_GTAC'C
Cuts at: 4972 4972
Size: 7952
MfeI C'AATT_G
Cuts at: 3419 3957 3419
Size: 538 7414
MluI A'CGCG_T
Cuts at: 7385 7385
Size: 7952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 228 497 1898 2288 2351 3231 3871 4216 4720
Size: 269 1401 390 63 880 640 345 504
Cuts at: 4720 6623 6807 228
Size: 1903 184 1373
Fragments arranged by size:
1903 1401 1373 880 640 504 390 345
269 184 63
MscI TGG'CCA
Cuts at: 657 1581 2696 5713 657
Size: 924 1115 3017 2896
Fragments arranged by size:
3017 2896 1115 924
MslI CAynn'nnrTG
Cuts at: 1322 3223 3725 3947 4261 5941 6068 1322
Size: 1901 502 222 314 1680 127 3206
Fragments arranged by size:
3206 1901 1680 502 314 222 127
MspA1I CmG'CkG
Cuts at: 268 1821 4103 5063 5377 5737 6750 6995 268
Size: 1553 2282 960 314 360 1013 245 1225
Fragments arranged by size:
2282 1553 1225 1013 960 360 314 245
NaeI GCC'GGC
Cuts at: 6133 6133
Size: 7952
NarI GG'CG_CC
Cuts at: 5383 5631 5383
Size: 248 7704
NcoI C'CATG_G
Cuts at: 107 911 1472 107
Size: 804 561 6587
Fragments arranged by size:
6587 804 561
NgoMIV G'CCGG_C
Cuts at: 6131 6131
Size: 7952
NspI r_CATG'y
Cuts at: 413 925 1488 2203 3854 5005 5505 6412 413
Size: 512 563 715 1651 1151 500 907 1953
Fragments arranged by size:
1953 1651 1151 907 715 563 512 500
PciI A'CATG_T
Cuts at: 1484 2199 3850 6408 1484
Size: 715 1651 2558 3028
Fragments arranged by size:
3028 2558 1651 715
PflMI CCAn_nnn'nTGG
Cuts at: 2083 2083
Size: 7952
PfoI T'CCnGG_A
Cuts at: 1055 4416 1055
Size: 3361 4591
PmeI GTTT'AAAC
Cuts at: 4951 4951
Size: 7952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3967 3999 7091 7123 3967
Size: 32 3092 32 4796
Fragments arranged by size:
4796 3092 32 32
PpuMI rG'GwC_Cy
Cuts at: 3390 3390
Size: 7952
PshAI GACnn'nnGTC
Cuts at: 1184 1184
Size: 7952
PsiI TTA'TAA
Cuts at: 634 634
Size: 7952
PspXI vC'TCGA_Gb
Cuts at: 3284 3284
Size: 7952
PstI C_TGCA'G
Cuts at: 142 1172 4705 4999 5684 142
Size: 1030 3533 294 685 2410
Fragments arranged by size:
3533 2410 1030 685 294
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7952
PvuII CAG'CTG
Cuts at: 268 1821 5377 5737 268
Size: 1553 3556 360 2483
Fragments arranged by size:
3556 2483 1553 360
RsrII CG'GwC_CG
Cuts at: 6147 6147
Size: 7952
SacI G_AGCT'C
Cuts at: 2364 3124 4966 2364
Size: 760 1842 5350
Fragments arranged by size:
5350 1842 760
SalI G'TCGA_C
Cuts at: 4989 4989
Size: 7952
SapI GCTCTTCn'nnn_
Cuts at: 2923 5975 6185 2923
Size: 3052 210 4690
Fragments arranged by size:
4690 3052 210
SbfI CC_TGCA'GG
Cuts at: 4999 4999
Size: 7952
ScaI AGT'ACT
Cuts at: 1685 7469 1685
Size: 5784 2168
SexAI A'CCwGG_T
Cuts at: 3806 3806
Size: 7952
SfcI C'TryA_G
Cuts at: 33 138 1168 1920 4656 4701 4995 5680 6673
Size: 105 1030 752 2736 45 294 685 993
Cuts at: 6673 6864 33
Size: 191 1121
Fragments arranged by size:
2736 1121 1030 993 752 685 294 191
105 45
SfoI GGC'GCC
Cuts at: 5384 5632 5384
Size: 248 7704
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7952
SmaI CCC'GGG
Cuts at: 1453 4974 1453
Size: 3521 4431
SmlI C'TyrA_G
Cuts at: 1122 2155 3284 3408 4427 5107 6022 6514 6776
Size: 1033 1129 124 1019 680 915 492 262
Cuts at: 6776 1122
Size: 2298
Fragments arranged by size:
2298 1129 1033 1019 915 680 492 262
124
SpeI A'CTAG_T
Cuts at: 67 1686 67
Size: 1619 6333
SphI G_CATG'C
Cuts at: 5005 5505 5005
Size: 500 7452
StyI C'CwwG_G
Cuts at: 107 911 1472 1595 4766 5086 5440 107
Size: 804 561 123 3171 320 354 2619
Fragments arranged by size:
3171 2619 804 561 354 320 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1720 4406 4505 5535 5668 6127 6336 1720
Size: 2686 99 1030 133 459 209 3336
Fragments arranged by size:
3336 2686 1030 459 209 133 99
TatI w'GTAC_w
Cuts at: 434 563 818 1683 2264 4933 7467 434
Size: 129 255 865 581 2669 2534 919
Fragments arranged by size:
2669 2534 919 865 581 255 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2769 2801 2880 2912 3545 3577 7469 7501 2769
Size: 32 79 32 633 32 3892 32 3220
Fragments arranged by size:
3892 3220 633 79 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5749 5749
Size: 7952
XbaI T'CTAG_A
Cuts at: 4983 4983
Size: 7952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2943 3994 5447 2943
Size: 1051 1453 5448
Fragments arranged by size:
5448 1453 1051
XhoI C'TCGA_G
Cuts at: 3284 4427 3284
Size: 1143 6809
XmaI C'CCGG_G
Cuts at: 1451 4972 1451
Size: 3521 4431
XmnI GAAnn'nnTTC
Cuts at: 1778 1778
Size: 7952
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI ApaI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BssHII BstEII ClaI DraIII
FseI FspAI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PspOMI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI