Restriction map for
pF1KE3344
(Circular) (Six-base) MAPSORT of: pF1KE3344.seq Check: 1732 from: 1 to: 6125
pF1KE3344 6125 bp
With 182 enzymes: SgfI *
August 14, 2013 11:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1260 2184 1260
Size: 924 5201
AatII G_ACGT'C
Cuts at: 404 2822 404
Size: 2418 3707
AccI GT'mk_AC
Cuts at: 404 3163 404
Size: 2759 3366
Acc65I G'GTAC_C
Cuts at: 3141 3141
Size: 6125
AclI AA'CG_TT
Cuts at: 5965 5965
Size: 6125
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 403 426 709 814 928 3950 4382 5129
Size: 45 23 283 105 114 3022 432 747
Cuts at: 5129 5397 358
Size: 268 1086
Fragments arranged by size:
3022 1086 747 432 283 268 114 105
45 23
AflIII A'CryG_T
Cuts at: 3477 4581 5558 3477
Size: 1104 977 4044
Fragments arranged by size:
4044 1104 977
AgeI A'CCGG_T
Cuts at: 4513 4513
Size: 6125
AhdI GACnn_n'nnGTC
Cuts at: 879 879
Size: 6125
AleI CACnn'nnGTG
Cuts at: 2545 2545
Size: 6125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 748 748
Size: 6125
AlwNI CAG_nnn'CTG
Cuts at: 573 804 858 978 1008 1095 1242 1398 1439
Size: 231 54 120 30 87 147 156 41
Cuts at: 1439 1557 1610 2109 2415 2837 2955 3430 4997
Size: 118 53 499 306 422 118 475 1567
Cuts at: 4997 573
Size: 1701
Fragments arranged by size:
1701 1567 499 475 422 306 231 156
147 120 118 118 87 54 53 41
30
ApaLI G'TGCA_C
Cuts at: 2373 4895 2373
Size: 2522 3603
ApoI r'AATT_y
Cuts at: 3129 5478 5734 3129
Size: 2349 256 3520
Fragments arranged by size:
3520 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6125
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6125
AvaI C'yCGr_G
Cuts at: 344 1635 1905 1938 2130 3116 3145 344
Size: 1291 270 33 192 986 29 3324
Fragments arranged by size:
3324 1291 986 270 192 33 29
BamHI G'GATC_C
Cuts at: 1799 3150 1799
Size: 1351 4774
BanI G'GyrC_C
Cuts at: 563 1942 2305 2547 2935 2994 3141 3555 3803
Size: 1379 363 242 388 59 147 414 248
Cuts at: 3803 3838 563
Size: 35 2850
Fragments arranged by size:
2850 1379 414 388 363 248 242 147
59 35
BanII G_rGCy'C
Cuts at: 1376 1644 1688 1912 1982 2331 2509 3115 3139
Size: 268 44 224 70 349 178 606 24
Cuts at: 3139 4169 1376
Size: 1030 3332
Fragments arranged by size:
3332 1030 606 349 268 224 178 70
44 24
BbeI G_GCGC'C
Cuts at: 2998 3559 3807 2998
Size: 561 248 5316
Fragments arranged by size:
5316 561 248
BbsI GAAGACnn'nnnn_
Cuts at: 346 346
Size: 6125
BbvCI CC'TCA_GC
Cuts at: 682 682
Size: 6125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 586 620 586
Size: 34 6091
BciVI GTATCCnnnnn_n'
Cuts at: 2283 4023 4784 6125 2283
Size: 1740 761 1341 2283
Fragments arranged by size:
2283 1740 1341 761
BclI T'GATC_A
Cuts at: 3645 3645
Size: 6125
BfrBI ATG'CAT
Cuts at: 1410 1840 1410
Size: 430 5695
BglI GCCn_nnn'nGGC
Cuts at: 313 2122 313
Size: 1809 4316
BglII A'GATC_T
Cuts at: 3640 5677 3640
Size: 2037 4088
BlpI GC'TnA_GC
Cuts at: 703 2330 3052 3237 703
Size: 1627 722 185 3591
Fragments arranged by size:
3591 1627 722 185
Bme1580I G_kGCm'C
Cuts at: 323 1945 2377 2552 2609 2938 3750 3843 4899
Size: 1622 432 175 57 329 812 93 1056
Cuts at: 4899 323
Size: 1549
Fragments arranged by size:
1622 1549 1056 812 432 329 175 93
57
BmgBI CAC'GTC
Cuts at: 1493 2220 2880 1493
Size: 727 660 4738
Fragments arranged by size:
4738 727 660
BmrI ACTGGGnnnn_n'
Cuts at: 1083 3337 3418 3753 5897 1083
Size: 2254 81 335 2144 1311
Fragments arranged by size:
2254 2144 1311 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1272 4492 1272
Size: 3220 2905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 646 988 1509 1636 1867 2020 2035 2086
Size: 252 342 521 127 231 153 15 51
Cuts at: 2086 2131 2307 2493 2727 2977 3051 394
Size: 45 176 186 234 250 74 3468
Fragments arranged by size:
3468 521 342 252 250 234 231 186
176 153 127 74 51 45 15
Bpu10I CC'TnA_GC
Cuts at: 682 1655 1804 2629 682
Size: 973 149 825 4178
Fragments arranged by size:
4178 973 825 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1333 2703 2896 3301 4180 4672 4970 1333
Size: 1370 193 405 879 492 298 2488
Fragments arranged by size:
2488 1370 879 492 405 298 193
BsaI GGTCTCn'nnnn_
Cuts at: 463 592 1184 1625 2975 5829 463
Size: 129 592 441 1350 2854 759
Fragments arranged by size:
2854 1350 759 592 441 129
BsaAI yAC'GTr
Cuts at: 2516 3478 2516
Size: 962 5163
BsaBI GATnn'nnATC
Cuts at: 6 2857 3639 5676 6
Size: 2851 782 2037 455
Fragments arranged by size:
2851 2037 782 455
BsaHI Gr'CG_yC
Cuts at: 401 1769 2819 2995 3556 3804 4506 6002 401
Size: 1368 1050 176 561 248 702 1496 524
Fragments arranged by size:
1496 1368 1050 702 561 524 248 176
BsaWI w'CCGG_w
Cuts at: 3314 3835 4513 4787 4934 5345 3314
Size: 521 678 274 147 411 4094
Fragments arranged by size:
4094 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 148 178 769 799 2630 2660 148
Size: 30 591 30 1831 30 3613
Fragments arranged by size:
3613 1831 591 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 283 286 532 793 907 928 2032 2590
Size: 114 3 246 261 114 21 1104 558
Cuts at: 2590 2638 2707 2959 2989 169
Size: 48 69 252 30 3305
Fragments arranged by size:
3305 1104 558 261 252 246 114 114
69 48 30 21 3
BseYI C'CCAG_C
Cuts at: 214 493 1543 2491 2759 3550 4308 4885 5381
Size: 279 1050 948 268 791 758 577 496
Cuts at: 5381 214
Size: 958
Fragments arranged by size:
1050 958 948 791 758 577 496 279
268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 601 700 2200 2823 2830 265
Size: 336 99 1500 623 7 3560
Fragments arranged by size:
3560 1500 623 336 99 7
BsiEI CG_ry'CG
Cuts at: 104 3667 3713 4921 104
Size: 3563 46 1208 1308
Fragments arranged by size:
3563 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 584 1221 1349 1688 2377 2600 3139 3917 4107
Size: 637 128 339 689 223 539 778 190
Cuts at: 4107 4899 584
Size: 792 1810
Fragments arranged by size:
1810 792 778 689 637 539 339 223
190 128
BsiWI C'GTAC_G
Cuts at: 198 198
Size: 6125
BsmI GAATG_Cn'
Cuts at: 1155 1155
Size: 6125
BsmBI CGTCTCn'nnnn_
Cuts at: 1421 2886 1421
Size: 1465 4660
Bsp1286I G_dGCh'C
Cuts at: 323 584 1221 1349 1376 1644 1688 1912 1945
Size: 261 637 128 27 268 44 224 33
Cuts at: 1945 1982 2331 2377 2509 2552 2600 2609 2938
Size: 37 349 46 132 43 48 9 329
Cuts at: 2938 3115 3139 3750 3843 3917 4107 4169 4899
Size: 177 24 611 93 74 190 62 730
Cuts at: 4899 323
Size: 1549
Fragments arranged by size:
1549 730 637 611 349 329 268 261
224 190 177 132 128 93 74 62
48 46 44 43 37 33 27 24
9
BspEI T'CCGG_A
Cuts at: 5345 5345
Size: 6125
BspHI T'CATG_A
Cuts at: 5301 5301
Size: 6125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 1260 2184 3175 3389 3691 4072 242
Size: 1018 924 991 214 302 381 2295
Fragments arranged by size:
2295 1018 991 924 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1038 1523 1766 4417 4471 5955 6120 50
Size: 988 485 243 2651 54 1484 165 55
Fragments arranged by size:
2651 1484 988 485 243 165 55 54
BsrDI GCAATG_nn'
Cuts at: 732 1840 2199 4037 732
Size: 1108 359 1838 2820
Fragments arranged by size:
2820 1838 1108 359
BsrFI r'CCGG_y
Cuts at: 4123 4304 4513 4123
Size: 181 209 5735
Fragments arranged by size:
5735 209 181
BsrGI T'GTAC_A
Cuts at: 1559 1559
Size: 6125
BssSI C'ACGA_G
Cuts at: 2602 4396 4754 2602
Size: 1794 358 3973
Fragments arranged by size:
3973 1794 358
BstAPI GCAn_nnn'nTGC
Cuts at: 804 3502 804
Size: 2698 3427
BstBI TT'CG_AA
Cuts at: 4486 4486
Size: 6125
BstEII G'GTnAC_C
Cuts at: 414 1812 1899 2763 414
Size: 1398 87 864 3776
Fragments arranged by size:
3776 1398 864 87
BstXI CCAn_nnnn'nTGG
Cuts at: 2499 2499
Size: 6125
BstYI r'GATC_y
Cuts at: 1 1619 1799 2624 3150 3617 3640 3975 4221
Size: 1618 180 825 526 467 23 335 246
Cuts at: 4221 5233 5319 5331 5677 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1618 1012 825 526 467 449 346 335
246 180 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 859 859
Size: 6125
BtgI C'CryG_G
Cuts at: 107 1043 1381 2024 2165 107
Size: 936 338 643 141 4067
Fragments arranged by size:
4067 936 643 338 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 348 3057 4075 4256 4419 348
Size: 2709 1018 181 163 2054
Fragments arranged by size:
2709 2054 1018 181 163
BtsI GCAGTG_nn'
Cuts at: 2377 3368 2377
Size: 991 5134
DraI TTT'AAA
Cuts at: 3124 3124
Size: 6125
DraIII CAC_nnn'GTG
Cuts at: 2268 2268
Size: 6125
DrdI GACnn_nn'nnGTC
Cuts at: 3831 4689 3831
Size: 858 5267
EaeI y'GGCC_r
Cuts at: 593 749 1571 2162 3710 3884 4275 4302 5647
Size: 156 822 591 1548 174 391 27 1345
Cuts at: 5647 593
Size: 1071
Fragments arranged by size:
1548 1345 1071 822 591 391 174 156
27
EagI C'GGCC_G
Cuts at: 3710 3710
Size: 6125
EarI CTCTTCn'nnn_
Cuts at: 1073 1105 4148 4358 1073
Size: 32 3043 210 2840
Fragments arranged by size:
3043 2840 210 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 967 1797 1933 3052 4333 4643 4789 5935 967
Size: 830 136 1119 1281 310 146 1146 1157
Fragments arranged by size:
1281 1157 1146 1119 830 310 146 136
EcoICRI GAG'CTC
Cuts at: 1686 3137 1686
Size: 1451 4674
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 394 403 426 646 709 814 928 988
Size: 36 9 23 220 63 105 114 60
Cuts at: 988 1509 1636 1867 2020 2035 2086 2131 2307
Size: 521 127 231 153 15 51 45 176
Cuts at: 2307 2493 2727 2977 3051 3950 4382 5129 5397
Size: 186 234 250 74 899 432 747 268
Cuts at: 5397 358
Size: 1086
Fragments arranged by size:
1086 899 747 521 432 268 250 234
231 220 186 176 153 127 114 105
74 63 60 51 45 36 23 15
9
EcoNI CCTnn'n_nnAGG
Cuts at: 238 514 775 910 982 1807 2017 2353 2472
Size: 276 261 135 72 825 210 336 119
Cuts at: 2472 238
Size: 3891
Fragments arranged by size:
3891 825 336 276 261 210 135 119
72
EcoO109I rG'GnC_Cy
Cuts at: 612 2106 2211 2919 3264 612
Size: 1494 105 708 345 3473
Fragments arranged by size:
3473 1494 708 345 105
EcoRI G'AATT_C
Cuts at: 3129 3129
Size: 6125
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4144 4144
Size: 6125
FspI TGC'GCA
Cuts at: 1306 3906 1306
Size: 2600 3525
HaeII r_GCGC'y
Cuts at: 308 2998 3559 3807 4829 5808 308
Size: 2690 561 248 1022 979 625
Fragments arranged by size:
2690 1022 979 625 561 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 865 897 2708 2740 3967 3997 3999 4029 865
Size: 32 1811 32 1227 30 2 30 2961
Fragments arranged by size:
2961 1811 1227 32 32 30 30 2
HincII GTy'rAC
Cuts at: 438 2076 2157 2889 3164 438
Size: 1638 81 732 275 3399
Fragments arranged by size:
3399 1638 732 275 81
KasI G'GCGC_C
Cuts at: 2994 3555 3803 2994
Size: 561 248 5316
Fragments arranged by size:
5316 561 248
KpnI G_GTAC'C
Cuts at: 3145 3145
Size: 6125
MluI A'CGCG_T
Cuts at: 5558 5558
Size: 6125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1457 2465 4796 4980 1457
Size: 1008 2331 184 2602
Fragments arranged by size:
2602 2331 1008 184
MscI TGG'CCA
Cuts at: 2164 3886 2164
Size: 1722 4403
MslI CAynn'nnrTG
Cuts at: 671 2545 4114 4241 671
Size: 1874 1569 127 2555
Fragments arranged by size:
2555 1874 1569 127
MspA1I CmG'CkG
Cuts at: 261 573 624 1323 1395 1610 1713 2756 2826
Size: 312 51 699 72 215 103 1043 70
Cuts at: 2826 2928 3056 3236 3550 3910 4923 5168 261
Size: 102 128 180 314 360 1013 245 1218
Fragments arranged by size:
1218 1043 1013 699 360 314 312 245
215 180 128 103 102 72 70 51
NaeI GCC'GGC
Cuts at: 4306 4306
Size: 6125
NarI GG'CG_CC
Cuts at: 2995 3556 3804 2995
Size: 561 248 5316
Fragments arranged by size:
5316 561 248
NcoI C'CATG_G
Cuts at: 107 2165 107
Size: 2058 4067
NgoMIV G'CCGG_C
Cuts at: 4304 4304
Size: 6125
NsiI A_TGCA'T
Cuts at: 1412 1842 1412
Size: 430 5695
NspI r_CATG'y
Cuts at: 1414 2260 3178 3678 4585 1414
Size: 846 918 500 907 2954
Fragments arranged by size:
2954 918 907 846 500
PasI CC'CwG_GG
Cuts at: 2609 2609
Size: 6125
PciI A'CATG_T
Cuts at: 4581 4581
Size: 6125
PflMI CCAn_nnn'nTGG
Cuts at: 1242 2109 2398 2778 1242
Size: 867 289 380 4589
Fragments arranged by size:
4589 867 380 289
PfoI T'CCnGG_A
Cuts at: 371 1622 1997 2102 2354 2729 2813 2954 371
Size: 1251 375 105 252 375 84 141 3542
Fragments arranged by size:
3542 1251 375 375 252 141 105 84
PmeI GTTT'AAAC
Cuts at: 3124 3124
Size: 6125
PmlI CAC'GTG
Cuts at: 2516 2516
Size: 6125
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1969 2001 5264 5296 1969
Size: 32 3263 32 2798
Fragments arranged by size:
3263 2798 32 32
PpuMI rG'GwC_Cy
Cuts at: 2106 2211 2106
Size: 105 6020
PsiI TTA'TAA
Cuts at: 2090 2090
Size: 6125
PspXI vC'TCGA_Gb
Cuts at: 344 344
Size: 6125
PstI C_TGCA'G
Cuts at: 428 515 578 737 776 809 1103 1160 1181
Size: 87 63 159 39 33 294 57 21
Cuts at: 1181 1328 1444 1730 2195 3172 3857 428
Size: 147 116 286 465 977 685 2696
Fragments arranged by size:
2696 977 685 465 294 286 159 147
116 87 63 57 39 33 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6125
PvuII CAG'CTG
Cuts at: 261 573 624 1323 1610 1713 2826 3056 3550
Size: 312 51 699 287 103 1113 230 494
Cuts at: 3550 3910 261
Size: 360 2476
Fragments arranged by size:
2476 1113 699 494 360 312 287 230
103 51
RsrII CG'GwC_CG
Cuts at: 4320 4320
Size: 6125
SacI G_AGCT'C
Cuts at: 1688 3139 1688
Size: 1451 4674
SalI G'TCGA_C
Cuts at: 3162 3162
Size: 6125
SapI GCTCTTCn'nnn_
Cuts at: 4148 4358 4148
Size: 210 5915
SbfI CC_TGCA'GG
Cuts at: 515 776 3172 515
Size: 261 2396 3468
Fragments arranged by size:
3468 2396 261
ScaI AGT'ACT
Cuts at: 1072 2680 5642 1072
Size: 1608 2962 1555
Fragments arranged by size:
2962 1608 1555
SexAI A'CCwGG_T
Cuts at: 1289 1289
Size: 6125
SfcI C'TryA_G
Cuts at: 33 424 511 574 720 733 772 805 1099
Size: 391 87 63 146 13 39 33 294
Cuts at: 1099 1134 1156 1177 1324 1440 1726 2191 2923
Size: 35 22 21 147 116 286 465 732
Cuts at: 2923 3168 3853 4846 5037 33
Size: 245 685 993 191 1121
Fragments arranged by size:
1121 993 732 685 465 391 294 286
245 191 147 146 116 87 63 39
35 33 22 21 13
SfoI GGC'GCC
Cuts at: 2996 3557 3805 2996
Size: 561 248 5316
Fragments arranged by size:
5316 561 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6125
SmaI CCC'GGG
Cuts at: 1637 1907 1940 3147 1637
Size: 270 33 1207 4615
Fragments arranged by size:
4615 1207 270 33
SmlI C'TyrA_G
Cuts at: 344 1348 2682 2911 3280 4195 4687 4949 344
Size: 1004 1334 229 369 915 492 262 1520
Fragments arranged by size:
1520 1334 1004 915 492 369 262 229
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6125
SphI G_CATG'C
Cuts at: 1414 2260 3178 3678 1414
Size: 846 918 500 3861
Fragments arranged by size:
3861 918 846 500
SrfI GCCC'GGGC
Cuts at: 1940 1940
Size: 6125
StuI AGG'CCT
Cuts at: 2704 2704
Size: 6125
StyI C'CwwG_G
Cuts at: 107 245 2165 2444 2652 3259 3613 107
Size: 138 1920 279 208 607 354 2619
Fragments arranged by size:
2619 1920 607 354 279 208 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 496 1960 2751 2818 3708 3841 4300 4509 496
Size: 1464 791 67 890 133 459 209 2112
Fragments arranged by size:
2112 1464 890 791 459 209 133 67
TatI w'GTAC_w
Cuts at: 1070 1559 2678 5640 1070
Size: 489 1119 2962 1555
Fragments arranged by size:
2962 1555 1119 489
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 148 180 5642 5674 148
Size: 32 5462 32 599
Fragments arranged by size:
5462 599 32 32
Tth111I GACn'n_nGTC
Cuts at: 1291 2833 3922 1291
Size: 1542 1089 3494
Fragments arranged by size:
3494 1542 1089
XbaI T'CTAG_A
Cuts at: 3156 3156
Size: 6125
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 369 1503 3620 369
Size: 1134 2117 2874
Fragments arranged by size:
2874 2117 1134
XhoI C'TCGA_G
Cuts at: 344 344
Size: 6125
XmaI C'CCGG_G
Cuts at: 1635 1905 1938 3145 1635
Size: 270 33 1207 4615
Fragments arranged by size:
4615 1207 270 33
ZraI GAC'GTC
Cuts at: 402 2820 402
Size: 2418 3707
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AloI ApaI AscI AvrII BaeI BmtI
BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PshAI
PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI SspI
SwaI XmnI