Restriction map for
pF1KE3341
(Circular) (Six-base) MAPSORT of: pF1KE3341.seq Check: 1472 from: 1 to: 6071
pF1KE3341 6071 bp
With 182 enzymes: SgfI *
August 18, 2011 15:44 ..
AccI GT'mk_AC
Cuts at: 182 3109 182
Size: 2927 3144
Acc65I G'GTAC_C
Cuts at: 870 3087 870
Size: 2217 3854
AclI AA'CG_TT
Cuts at: 5911 5911
Size: 6071
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1662 3896 4328 5075 5343 1662
Size: 2234 432 747 268 2390
Fragments arranged by size:
2390 2234 747 432 268
AflIII A'CryG_T
Cuts at: 2735 3423 4527 5504 2735
Size: 688 1104 977 3302
Fragments arranged by size:
3302 1104 977 688
AgeI A'CCGG_T
Cuts at: 2885 4459 2885
Size: 1574 4497
AleI CACnn'nnGTG
Cuts at: 574 574
Size: 6071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1795 1795
Size: 6071
AlwNI CAG_nnn'CTG
Cuts at: 769 1399 1663 2911 3376 4943 769
Size: 630 264 1248 465 1567 1897
Fragments arranged by size:
1897 1567 1248 630 465 264
ApaLI G'TGCA_C
Cuts at: 1026 2938 4841 1026
Size: 1912 1903 2256
Fragments arranged by size:
2256 1912 1903
ApoI r'AATT_y
Cuts at: 412 1999 2506 2716 2833 3075 5424 5680 412
Size: 1587 507 210 117 242 2349 256 803
Fragments arranged by size:
2349 1587 803 507 256 242 210 117
AseI AT'TA_AT
Cuts at: 20 20
Size: 6071
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6071
AvaI C'yCGr_G
Cuts at: 377 1767 2411 3091 377
Size: 1390 644 680 3357
Fragments arranged by size:
3357 1390 680 644
BamHI G'GATC_C
Cuts at: 2817 3096 2817
Size: 279 5792
BanI G'GyrC_C
Cuts at: 870 3087 3501 3749 3784 870
Size: 2217 414 248 35 3157
Fragments arranged by size:
3157 2217 414 248 35
BanII G_rGCy'C
Cuts at: 390 1688 2794 3085 4115 390
Size: 1298 1106 291 1030 2346
Fragments arranged by size:
2346 1298 1106 1030 291
BbeI G_GCGC'C
Cuts at: 3505 3753 3505
Size: 248 5823
BbsI GAAGACnn'nnnn_
Cuts at: 430 770 1294 430
Size: 340 524 5207
Fragments arranged by size:
5207 524 340
BciVI GTATCCnnnnn_n'
Cuts at: 3969 4730 6071 3969
Size: 761 1341 3969
Fragments arranged by size:
3969 1341 761
BclI T'GATC_A
Cuts at: 2195 3591 2195
Size: 1396 4675
BfrBI ATG'CAT
Cuts at: 2233 2233
Size: 6071
BglII A'GATC_T
Cuts at: 217 2048 3586 5623 217
Size: 1831 1538 2037 665
Fragments arranged by size:
2037 1831 1538 665
BlpI GC'TnA_GC
Cuts at: 3183 3183
Size: 6071
Bme1580I G_kGCm'C
Cuts at: 988 1030 2942 3696 3789 4845 988
Size: 42 1912 754 93 1056 2214
Fragments arranged by size:
2214 1912 1056 754 93 42
BmrI ACTGGGnnnn_n'
Cuts at: 738 3283 3364 3699 5843 738
Size: 2545 81 335 2144 966
Fragments arranged by size:
2545 2144 966 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1175 2238 4438 1175
Size: 1063 2200 2808
Fragments arranged by size:
2808 2200 1063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2778 2851 2778
Size: 73 5998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 441 2440 3247 4126 4618 4916 441
Size: 1999 807 879 492 298 1596
Fragments arranged by size:
1999 1596 879 807 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1764 5775 1764
Size: 4011 2060
BsaAI yAC'GTr
Cuts at: 1644 1910 2943 3424 1644
Size: 266 1033 481 4291
Fragments arranged by size:
4291 1033 481 266
BsaBI GATnn'nnATC
Cuts at: 6 2467 3585 5622 6
Size: 2461 1118 2037 455
Fragments arranged by size:
2461 2037 1118 455
BsaHI Gr'CG_yC
Cuts at: 446 2022 3502 3750 4452 5948 446
Size: 1576 1480 248 702 1496 569
Fragments arranged by size:
1576 1496 1480 702 569 248
BsaWI w'CCGG_w
Cuts at: 2430 2471 2885 3260 3781 4459 4733 4880 5291
Size: 41 414 375 521 678 274 147 411
Cuts at: 5291 2430
Size: 3210
Fragments arranged by size:
3210 678 521 414 411 375 274 147
41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1812 1842 1812
Size: 30 6041
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 922 2112 2715 922
Size: 1190 603 4278
Fragments arranged by size:
4278 1190 603
BseYI C'CCAG_C
Cuts at: 112 340 898 1228 3496 4254 4831 5327 112
Size: 228 558 330 2268 758 577 496 856
Fragments arranged by size:
2268 856 758 577 558 496 330 228
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1065 1065
Size: 6071
BsiEI CG_ry'CG
Cuts at: 104 3613 3659 4867 104
Size: 3509 46 1208 1308
Fragments arranged by size:
3509 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1030 2794 2942 3085 3863 4053 4845 1030
Size: 1764 148 143 778 190 792 2256
Fragments arranged by size:
2256 1764 792 778 190 148 143
BsiWI C'GTAC_G
Cuts at: 1910 2564 1910
Size: 654 5417
BsmI GAATG_Cn'
Cuts at: 928 2233 928
Size: 1305 4766
BsmBI CGTCTCn'nnnn_
Cuts at: 460 1815 2029 460
Size: 1355 214 4502
Fragments arranged by size:
4502 1355 214
Bsp1286I G_dGCh'C
Cuts at: 390 988 1030 1688 2794 2942 3085 3696 3789
Size: 598 42 658 1106 148 143 611 93
Cuts at: 3789 3863 4053 4115 4845 390
Size: 74 190 62 730 1616
Fragments arranged by size:
1616 1106 730 658 611 598 190 148
143 93 74 62 42
BspEI T'CCGG_A
Cuts at: 2430 5291 2430
Size: 2861 3210
BspHI T'CATG_A
Cuts at: 596 5247 596
Size: 4651 1420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3121 3335 3637 4018 3121
Size: 214 302 381 5174
Fragments arranged by size:
5174 381 302 214
BsrBI CCG'CTC
Cuts at: 50 912 917 4363 4417 5901 6066 50
Size: 862 5 3446 54 1484 165 55
Fragments arranged by size:
3446 1484 862 165 55 54 5
BsrDI GCAATG_nn'
Cuts at: 333 548 1436 1796 2657 3026 3983 333
Size: 215 888 360 861 369 957 2421
Fragments arranged by size:
2421 957 888 861 369 360 215
BsrFI r'CCGG_y
Cuts at: 2885 4069 4250 4459 2885
Size: 1184 181 209 4497
Fragments arranged by size:
4497 1184 209 181
BssSI C'ACGA_G
Cuts at: 1023 4342 4700 1023
Size: 3319 358 2394
Fragments arranged by size:
3319 2394 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3042 3448 3042
Size: 406 5665
BstBI TT'CG_AA
Cuts at: 4432 4432
Size: 6071
BstEII G'GTnAC_C
Cuts at: 1222 2633 1222
Size: 1411 4660
BstXI CCAn_nnnn'nTGG
Cuts at: 1443 2127 2880 1443
Size: 684 753 4634
Fragments arranged by size:
4634 753 684
BstYI r'GATC_y
Cuts at: 1 217 614 1917 2048 2817 3096 3563 3586
Size: 216 397 1303 131 769 279 467 23
Cuts at: 3586 3921 4167 5179 5265 5277 5623 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1303 1012 769 467 449 397 346 335
279 246 216 131 86 23 12
BstZ17I GTA'TAC
Cuts at: 183 183
Size: 6071
Bsu36I CC'TnA_GG
Cuts at: 2239 2239
Size: 6071
BtgI C'CryG_G
Cuts at: 107 962 107
Size: 855 5216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 666 4021 4202 4365 666
Size: 3355 181 163 2372
Fragments arranged by size:
3355 2372 181 163
BtsI GCAGTG_nn'
Cuts at: 1779 1937 2022 2804 3015 3314 1779
Size: 158 85 782 211 299 4536
Fragments arranged by size:
4536 782 299 211 158 85
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1194 1229 1194
Size: 35 6036
DraI TTT'AAA
Cuts at: 3070 3070
Size: 6071
DraIII CAC_nnn'GTG
Cuts at: 1025 1025
Size: 6071
DrdI GACnn_nn'nnGTC
Cuts at: 3777 4635 3777
Size: 858 5213
EaeI y'GGCC_r
Cuts at: 1173 2348 3656 3830 4221 4248 5593 1173
Size: 1175 1308 174 391 27 1345 1651
Fragments arranged by size:
1651 1345 1308 1175 391 174 27
EagI C'GGCC_G
Cuts at: 3656 3656
Size: 6071
EarI CTCTTCn'nnn_
Cuts at: 151 815 1856 2767 4094 4304 151
Size: 664 1041 911 1327 210 1918
Fragments arranged by size:
1918 1327 1041 911 664 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4279 4589 4735 5881 4279
Size: 310 146 1146 4469
Fragments arranged by size:
4469 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2792 3083 2792
Size: 291 5780
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1662 2778 2851 3896 4328 5075 5343 1662
Size: 1116 73 1045 432 747 268 2390
Fragments arranged by size:
2390 1116 1045 747 432 268 73
EcoNI CCTnn'n_nnAGG
Cuts at: 257 2113 257
Size: 1856 4215
EcoO109I rG'GnC_Cy
Cuts at: 674 2576 2955 3210 674
Size: 1902 379 255 3535
Fragments arranged by size:
3535 1902 379 255
EcoRI G'AATT_C
Cuts at: 2833 3075 2833
Size: 242 5829
EcoRV GAT'ATC
Cuts at: 1605 1605
Size: 6071
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2916 4090 2916
Size: 1174 4897
FspI TGC'GCA
Cuts at: 3852 3852
Size: 6071
HaeII r_GCGC'y
Cuts at: 3505 3753 4775 5754 3505
Size: 248 1022 979 3822
Fragments arranged by size:
3822 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 202 234 1054 1086 2249 2281 2809 2841 3913
Size: 32 820 32 1163 32 528 32 1072
Cuts at: 3913 3943 3945 3975 202
Size: 30 2 30 2298
Fragments arranged by size:
2298 1163 1072 820 528 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 810 1125 2733 3110 810
Size: 315 1608 377 3771
Fragments arranged by size:
3771 1608 377 315
HindIII A'AGCT_T
Cuts at: 929 929
Size: 6071
KasI G'GCGC_C
Cuts at: 3501 3749 3501
Size: 248 5823
KpnI G_GTAC'C
Cuts at: 874 3091 874
Size: 2217 3854
MfeI C'AATT_G
Cuts at: 2492 2492
Size: 6071
MluI A'CGCG_T
Cuts at: 5504 5504
Size: 6071
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2720 2795 4742 4926 2720
Size: 75 1947 184 3865
Fragments arranged by size:
3865 1947 184 75
MscI TGG'CCA
Cuts at: 3832 3832
Size: 6071
MslI CAynn'nnrTG
Cuts at: 574 2365 2878 4060 4187 574
Size: 1791 513 1182 127 2458
Fragments arranged by size:
2458 1791 1182 513 127
MspA1I CmG'CkG
Cuts at: 1588 1663 2274 2673 2908 3182 3496 3856 4869
Size: 75 611 399 235 274 314 360 1013
Cuts at: 4869 5114 1588
Size: 245 2545
Fragments arranged by size:
2545 1013 611 399 360 314 274 245
235 75
NaeI GCC'GGC
Cuts at: 4252 4252
Size: 6071
NarI GG'CG_CC
Cuts at: 3502 3750 3502
Size: 248 5823
NcoI C'CATG_G
Cuts at: 107 107
Size: 6071
NdeI CA'TA_TG
Cuts at: 1893 1893
Size: 6071
NgoMIV G'CCGG_C
Cuts at: 4250 4250
Size: 6071
NsiI A_TGCA'T
Cuts at: 2235 2235
Size: 6071
NspI r_CATG'y
Cuts at: 2286 2409 2739 3124 3624 4531 2286
Size: 123 330 385 500 907 3826
Fragments arranged by size:
3826 907 500 385 330 123
PciI A'CATG_T
Cuts at: 2735 4527 2735
Size: 1792 4279
PfoI T'CCnGG_A
Cuts at: 617 1520 1717 617
Size: 903 197 4971
Fragments arranged by size:
4971 903 197
PmeI GTTT'AAAC
Cuts at: 3070 3070
Size: 6071
PmlI CAC'GTG
Cuts at: 1644 2943 1644
Size: 1299 4772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5210 5242 5210
Size: 32 6039
PpuMI rG'GwC_Cy
Cuts at: 2955 2955
Size: 6071
PstI C_TGCA'G
Cuts at: 3118 3803 3118
Size: 685 5386
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6071
PvuII CAG'CTG
Cuts at: 1588 1663 2274 2673 2908 3496 3856 1588
Size: 75 611 399 235 588 360 3803
Fragments arranged by size:
3803 611 588 399 360 235 75
RsrII CG'GwC_CG
Cuts at: 4266 4266
Size: 6071
SacI G_AGCT'C
Cuts at: 2794 3085 2794
Size: 291 5780
SalI G'TCGA_C
Cuts at: 3108 3108
Size: 6071
SapI GCTCTTCn'nnn_
Cuts at: 4094 4304 4094
Size: 210 5861
SbfI CC_TGCA'GG
Cuts at: 3118 3118
Size: 6071
ScaI AGT'ACT
Cuts at: 5588 5588
Size: 6071
SfcI C'TryA_G
Cuts at: 33 3114 3799 4792 4983 33
Size: 3081 685 993 191 1121
Fragments arranged by size:
3081 1121 993 685 191
SfoI GGC'GCC
Cuts at: 3503 3751 3503
Size: 248 5823
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6071
SmaI CCC'GGG
Cuts at: 3093 3093
Size: 6071
SmlI C'TyrA_G
Cuts at: 420 2419 3226 4141 4633 4895 420
Size: 1999 807 915 492 262 1596
Fragments arranged by size:
1999 1596 915 807 492 262
SnaBI TAC'GTA
Cuts at: 1910 1910
Size: 6071
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6071
SphI G_CATG'C
Cuts at: 2286 3124 3624 2286
Size: 838 500 4733
Fragments arranged by size:
4733 838 500
StuI AGG'CCT
Cuts at: 1613 2712 1613
Size: 1099 4972
StyI C'CwwG_G
Cuts at: 107 1688 2225 3205 3559 107
Size: 1581 537 980 354 2619
Fragments arranged by size:
2619 1581 980 537 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 227 586 1052 3654 3787 4246 4455 227
Size: 359 466 2602 133 459 209 1843
Fragments arranged by size:
2602 1843 466 459 359 209 133
TatI w'GTAC_w
Cuts at: 479 842 2207 5586 479
Size: 363 1365 3379 964
Fragments arranged by size:
3379 1365 964 363
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 250 282 713 745 2345 2377 5588 5620 250
Size: 32 431 32 1600 32 3211 32 701
Fragments arranged by size:
3211 1600 701 431 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 163 3868 163
Size: 3705 2366
XbaI T'CTAG_A
Cuts at: 3102 3102
Size: 6071
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 580 1752 3566 580
Size: 1172 1814 3085
Fragments arranged by size:
3085 1814 1172
XmaI C'CCGG_G
Cuts at: 3091 3091
Size: 6071
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PfoI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsrGI BssHII ClaI FseI FspAI HpaI NheI NotI
NruI PacI PasI PflMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
SwaI XhoI XmnI ZraI