Restriction map for
pF1KE3330
(Circular) (Six-base) MAPSORT of: pF1KE3330.seq Check: 7596 from: 1 to: 5942
pF1KE3330 5942 bp
With 182 enzymes: SgfI *
March 2, 2011 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2223 2646 2895 2223
Size: 423 249 5270
Fragments arranged by size:
5270 423 249
AccI GT'mk_AC
Cuts at: 754 1568 2980 754
Size: 814 1412 3716
Fragments arranged by size:
3716 1412 814
Acc65I G'GTAC_C
Cuts at: 2958 2958
Size: 5942
AclI AA'CG_TT
Cuts at: 5782 5782
Size: 5942
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 679 934 1654 1749 2148 3767 4199 4946
Size: 494 255 720 95 399 1619 432 747
Cuts at: 4946 5214 185
Size: 268 913
Fragments arranged by size:
1619 913 747 720 494 432 399 268
255 95
AflIII A'CryG_T
Cuts at: 3294 4398 5375 3294
Size: 1104 977 3861
Fragments arranged by size:
3861 1104 977
AgeI A'CCGG_T
Cuts at: 1553 4330 1553
Size: 2777 3165
AhdI GACnn_n'nnGTC
Cuts at: 2133 2133
Size: 5942
AleI CACnn'nnGTG
Cuts at: 2696 2696
Size: 5942
AlwNI CAG_nnn'CTG
Cuts at: 480 496 1752 1772 2238 2875 3247 4814 480
Size: 16 1256 20 466 637 372 1567 1608
Fragments arranged by size:
1608 1567 1256 637 466 372 20 16
ApaI G_GGCC'C
Cuts at: 1131 1231 1655 2474 2823 1131
Size: 100 424 819 349 4250
Fragments arranged by size:
4250 819 424 349 100
ApaLI G'TGCA_C
Cuts at: 653 1885 4712 653
Size: 1232 2827 1883
Fragments arranged by size:
2827 1883 1232
ApoI r'AATT_y
Cuts at: 294 1795 2946 5295 5551 294
Size: 1501 1151 2349 256 685
Fragments arranged by size:
2349 1501 1151 685 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5942
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5942
AvaI C'yCGr_G
Cuts at: 113 836 1295 1596 2391 2962 113
Size: 723 459 301 795 571 3093
Fragments arranged by size:
3093 795 723 571 459 301
BamHI G'GATC_C
Cuts at: 222 2967 222
Size: 2745 3197
BanI G'GyrC_C
Cuts at: 840 2541 2586 2658 2958 3372 3620 3655 840
Size: 1701 45 72 300 414 248 35 3127
Fragments arranged by size:
3127 1701 414 300 248 72 45 35
BanII G_rGCy'C
Cuts at: 121 991 1131 1231 1655 1874 2474 2823 2956
Size: 870 140 100 424 219 600 349 133
Cuts at: 2956 3986 121
Size: 1030 2077
Fragments arranged by size:
2077 1030 870 600 424 349 219 140
133 100
BbeI G_GCGC'C
Cuts at: 3376 3624 3376
Size: 248 5694
BbsI GAAGACnn'nnnn_
Cuts at: 451 538 2154 451
Size: 87 1616 4239
Fragments arranged by size:
4239 1616 87
BciVI GTATCCnnnnn_n'
Cuts at: 3840 4601 5942 3840
Size: 761 1341 3840
Fragments arranged by size:
3840 1341 761
BclI T'GATC_A
Cuts at: 3462 3462
Size: 5942
BglI GCCn_nnn'nGGC
Cuts at: 163 1727 2092 2599 163
Size: 1564 365 507 3506
Fragments arranged by size:
3506 1564 507 365
BglII A'GATC_T
Cuts at: 1997 3457 5494 1997
Size: 1460 2037 2445
Fragments arranged by size:
2445 2037 1460
BlpI GC'TnA_GC
Cuts at: 3054 3054
Size: 5942
Bme1580I G_kGCm'C
Cuts at: 657 843 1131 1193 1231 1655 1889 2474 2546
Size: 186 288 62 38 424 234 585 72
Cuts at: 2546 2823 3567 3660 4716 657
Size: 277 744 93 1056 1883
Fragments arranged by size:
1883 1056 744 585 424 288 277 234
186 93 72 62 38
BmgBI CAC'GTC
Cuts at: 752 1827 752
Size: 1075 4867
BmrI ACTGGGnnnn_n'
Cuts at: 2431 2499 3154 3235 3570 5714 2431
Size: 68 655 81 335 2144 2659
Fragments arranged by size:
2659 2144 655 335 81 68
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2877 4309 2877
Size: 1432 4510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 904 1888 322
Size: 582 984 4376
Fragments arranged by size:
4376 984 582
Bpu10I CC'TnA_GC
Cuts at: 1065 1175 1753 2425 1065
Size: 110 578 672 4582
Fragments arranged by size:
4582 672 578 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3118 3997 4489 4787 3118
Size: 879 492 298 4273
Fragments arranged by size:
4273 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5646 5646
Size: 5942
BsaAI yAC'GTr
Cuts at: 1938 2466 3295 1938
Size: 528 829 4585
Fragments arranged by size:
4585 829 528
BsaBI GATnn'nnATC
Cuts at: 6 436 3456 5493 6
Size: 430 3020 2037 455
Fragments arranged by size:
3020 2037 455 430
BsaHI Gr'CG_yC
Cuts at: 551 3373 3621 4323 5819 551
Size: 2822 248 702 1496 674
Fragments arranged by size:
2822 1496 702 674 248
BsaWI w'CCGG_w
Cuts at: 440 671 1553 2222 3131 3652 4330 4604 4751
Size: 231 882 669 909 521 678 274 147
Cuts at: 4751 5162 440
Size: 411 1220
Fragments arranged by size:
1220 909 882 678 669 521 411 274
231 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 817 847 817
Size: 30 5912
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1552 1678 2654 2860 2863 2866 1552
Size: 126 976 206 3 3 4628
Fragments arranged by size:
4628 976 206 126 3 3
BseYI C'CCAG_C
Cuts at: 567 1023 1035 1336 1450 1542 1725 2052 2305
Size: 456 12 301 114 92 183 327 253
Cuts at: 2305 2683 2883 3367 4125 4702 5198 567
Size: 378 200 484 758 577 496 1311
Fragments arranged by size:
1311 758 577 496 484 456 378 327
301 253 200 183 114 92 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 436 2647 2854 334
Size: 102 2211 207 3422
Fragments arranged by size:
3422 2211 207 102
BsiEI CG_ry'CG
Cuts at: 104 139 158 170 3484 3530 4738 104
Size: 35 19 12 3314 46 1208 1308
Fragments arranged by size:
3314 1308 1208 46 35 19 12
BsiHKAI G_wGCw'C
Cuts at: 657 778 1695 1889 2431 2830 2956 3734 3924
Size: 121 917 194 542 399 126 778 190
Cuts at: 3924 4716 657
Size: 792 1883
Fragments arranged by size:
1883 917 792 778 542 399 194 190
126 121
BsiWI C'GTAC_G
Cuts at: 1257 1257
Size: 5942
Bsp1286I G_dGCh'C
Cuts at: 121 657 778 843 991 1131 1193 1231 1655
Size: 536 121 65 148 140 62 38 424
Cuts at: 1655 1695 1874 1889 2431 2474 2546 2823 2830
Size: 40 179 15 542 43 72 277 7
Cuts at: 2830 2956 3567 3660 3734 3924 3986 4716 121
Size: 126 611 93 74 190 62 730 1347
Fragments arranged by size:
1347 730 611 542 536 424 277 190
179 148 140 126 121 93 74 72
65 62 62 43 40 38 15 7
BspEI T'CCGG_A
Cuts at: 440 2222 5162 440
Size: 1782 2940 1220
Fragments arranged by size:
2940 1782 1220
BspHI T'CATG_A
Cuts at: 2279 5118 2279
Size: 2839 3103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2223 2615 2646 2895 2992 3206 3508 3889 2223
Size: 392 31 249 97 214 302 381 4276
Fragments arranged by size:
4276 392 381 302 249 214 97 31
BsrBI CCG'CTC
Cuts at: 50 923 933 4234 4288 5772 5937 50
Size: 873 10 3301 54 1484 165 55
Fragments arranged by size:
3301 1484 873 165 55 54 10
BsrDI GCAATG_nn'
Cuts at: 1971 2451 3854 1971
Size: 480 1403 4059
Fragments arranged by size:
4059 1403 480
BsrFI r'CCGG_y
Cuts at: 1553 2228 2309 2339 2583 2589 3940 4121 4330
Size: 675 81 30 244 6 1351 181 209
Cuts at: 4330 1553
Size: 3165
Fragments arranged by size:
3165 1351 675 244 209 181 81 30
6
BsrGI T'GTAC_A
Cuts at: 1322 1322
Size: 5942
BssHII G'CGCG_C
Cuts at: 829 829
Size: 5942
BssSI C'ACGA_G
Cuts at: 613 1284 4213 4571 613
Size: 671 2929 358 1984
Fragments arranged by size:
2929 1984 671 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2238 2658 3319 2238
Size: 420 661 4861
Fragments arranged by size:
4861 661 420
BstBI TT'CG_AA
Cuts at: 4303 4303
Size: 5942
BstEII G'GTnAC_C
Cuts at: 1576 1734 1576
Size: 158 5784
BstXI CCAn_nnnn'nTGG
Cuts at: 619 684 619
Size: 65 5877
BstYI r'GATC_y
Cuts at: 1 222 1997 2630 2967 3434 3457 3792 4038
Size: 221 1775 633 337 467 23 335 246
Cuts at: 4038 5050 5136 5148 5494 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1775 1012 633 467 449 346 337 335
246 221 86 23 12
BtgI C'CryG_G
Cuts at: 107 130 424 1123 2537 2732 2753 107
Size: 23 294 699 1414 195 21 3296
Fragments arranged by size:
3296 1414 699 294 195 23 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 900 1326 1344 3892 4073 4236 900
Size: 426 18 2548 181 163 2606
Fragments arranged by size:
2606 2548 426 181 163 18
BtsI GCAGTG_nn'
Cuts at: 351 2709 3185 351
Size: 2358 476 3108
Fragments arranged by size:
3108 2358 476
DraI TTT'AAA
Cuts at: 2941 2941
Size: 5942
DraIII CAC_nnn'GTG
Cuts at: 810 1893 1938 810
Size: 1083 45 4814
Fragments arranged by size:
4814 1083 45
DrdI GACnn_nn'nnGTC
Cuts at: 3648 4506 3648
Size: 858 5084
EaeI y'GGCC_r
Cuts at: 136 155 167 515 1619 1955 3527 3701 4092
Size: 19 12 348 1104 336 1572 174 391
Cuts at: 4092 4119 5464 136
Size: 27 1345 614
Fragments arranged by size:
1572 1345 1104 614 391 348 336 174
27 19 12
EagI C'GGCC_G
Cuts at: 136 155 167 3527 136
Size: 19 12 3360 2551
Fragments arranged by size:
3360 2551 19 12
EarI CTCTTCn'nnn_
Cuts at: 2020 3965 4175 2020
Size: 1945 210 3787
Fragments arranged by size:
3787 1945 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 444 1851 4150 4460 4606 5752 444
Size: 1407 2299 310 146 1146 634
Fragments arranged by size:
2299 1407 1146 634 310 146
EcoICRI GAG'CTC
Cuts at: 2954 2954
Size: 5942
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 322 679 904 934 1654 1749 1888 2148
Size: 137 357 225 30 720 95 139 260
Cuts at: 2148 3767 4199 4946 5214 185
Size: 1619 432 747 268 913
Fragments arranged by size:
1619 913 747 720 432 357 268 260
225 139 137 95 30
EcoNI CCTnn'n_nnAGG
Cuts at: 1660 2155 1660
Size: 495 5447
EcoO109I rG'GnC_Cy
Cuts at: 241 477 1128 1227 1228 1651 1652 1950 2470
Size: 236 651 99 1 423 1 298 520
Cuts at: 2470 2484 2600 2819 3081 241
Size: 14 116 219 262 3102
Fragments arranged by size:
3102 651 520 423 298 262 236 219
116 99 14 1 1
EcoRI G'AATT_C
Cuts at: 1795 2946 1795
Size: 1151 4791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3961 3961
Size: 5942
FspI TGC'GCA
Cuts at: 1012 3723 1012
Size: 2711 3231
FspAI rTGC'GCAy
Cuts at: 1012 1012
Size: 5942
HaeII r_GCGC'y
Cuts at: 127 973 2789 3376 3624 4646 5625 127
Size: 846 1816 587 248 1022 979 444
Fragments arranged by size:
1816 1022 979 846 587 444 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 258 290 455 487 760 792 817 849 1267
Size: 32 165 32 273 32 25 32 418
Cuts at: 1267 1299 1909 1941 2119 2151 3784 3814 3816
Size: 32 610 32 178 32 1633 30 2
Cuts at: 3816 3846 258
Size: 30 2354
Fragments arranged by size:
2354 1633 610 418 273 178 165 32
32 32 32 32 32 32 30 30
25 2
HincII GTy'rAC
Cuts at: 2571 2981 2571
Size: 410 5532
KasI G'GCGC_C
Cuts at: 3372 3620 3372
Size: 248 5694
KpnI G_GTAC'C
Cuts at: 2962 2962
Size: 5942
MluI A'CGCG_T
Cuts at: 5375 5375
Size: 5942
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 295 598 746 950 2222 2390 2768 4613 4797
Size: 303 148 204 1272 168 378 1845 184
Cuts at: 4797 295
Size: 1440
Fragments arranged by size:
1845 1440 1272 378 303 204 184 168
148
MscI TGG'CCA
Cuts at: 517 1621 1957 3703 517
Size: 1104 336 1746 2756
Fragments arranged by size:
2756 1746 1104 336
MslI CAynn'nnrTG
Cuts at: 617 682 940 1465 2515 2696 2752 3931 4058
Size: 65 258 525 1050 181 56 1179 127
Cuts at: 4058 617
Size: 2501
Fragments arranged by size:
2501 1179 1050 525 258 181 127 65
56
MspA1I CmG'CkG
Cuts at: 132 141 426 730 954 1428 1437 2078 2928
Size: 9 285 304 224 474 9 641 850
Cuts at: 2928 3053 3367 3727 4740 4985 132
Size: 125 314 360 1013 245 1089
Fragments arranged by size:
1089 1013 850 641 474 360 314 304
285 245 224 125 9 9
NaeI GCC'GGC
Cuts at: 2311 2341 2591 4123 2311
Size: 30 250 1532 4130
Fragments arranged by size:
4130 1532 250 30
NarI GG'CG_CC
Cuts at: 3373 3621 3373
Size: 248 5694
NcoI C'CATG_G
Cuts at: 107 2537 2753 107
Size: 2430 216 3296
Fragments arranged by size:
3296 2430 216
NgoMIV G'CCGG_C
Cuts at: 2309 2339 2589 4121 2309
Size: 30 250 1532 4130
Fragments arranged by size:
4130 1532 250 30
NotI GC'GGCC_GC
Cuts at: 136 167 136
Size: 31 5911
NspI r_CATG'y
Cuts at: 1011 1389 2995 3495 4402 1011
Size: 378 1606 500 907 2551
Fragments arranged by size:
2551 1606 907 500 378
PciI A'CATG_T
Cuts at: 4398 4398
Size: 5942
PflMI CCAn_nnn'nTGG
Cuts at: 480 513 1461 1617 1938 2208 2815 480
Size: 33 948 156 321 270 607 3607
Fragments arranged by size:
3607 948 607 321 270 156 33
PfoI T'CCnGG_A
Cuts at: 193 473 193
Size: 280 5662
PmeI GTTT'AAAC
Cuts at: 2941 2941
Size: 5942
PmlI CAC'GTG
Cuts at: 1938 1938
Size: 5942
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 607 639 5081 5113 607
Size: 32 4442 32 1436
Fragments arranged by size:
4442 1436 32 32
PpuMI rG'GwC_Cy
Cuts at: 241 477 1950 241
Size: 236 1473 4233
Fragments arranged by size:
4233 1473 236
PspOMI G'GGCC_C
Cuts at: 1127 1227 1651 2470 2819 1127
Size: 100 424 819 349 4250
Fragments arranged by size:
4250 819 424 349 100
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2095 2127 2095
Size: 32 5910
PstI C_TGCA'G
Cuts at: 1136 2180 2906 2989 3674 1136
Size: 1044 726 83 685 3404
Fragments arranged by size:
3404 1044 726 685 83
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5942
PvuII CAG'CTG
Cuts at: 954 1437 2928 3367 3727 954
Size: 483 1491 439 360 3169
Fragments arranged by size:
3169 1491 483 439 360
RsrII CG'GwC_CG
Cuts at: 4137 4137
Size: 5942
SacI G_AGCT'C
Cuts at: 2956 2956
Size: 5942
SacII CC_GC'GG
Cuts at: 133 427 133
Size: 294 5648
SalI G'TCGA_C
Cuts at: 2979 2979
Size: 5942
SapI GCTCTTCn'nnn_
Cuts at: 3965 4175 3965
Size: 210 5732
SbfI CC_TGCA'GG
Cuts at: 2989 2989
Size: 5942
ScaI AGT'ACT
Cuts at: 5459 5459
Size: 5942
SfcI C'TryA_G
Cuts at: 33 1132 2176 2902 2985 3670 4663 4854 33
Size: 1099 1044 726 83 685 993 191 1121
Fragments arranged by size:
1121 1099 1044 993 726 685 191 83
SfiI GGCCn_nnn'nGGCC
Cuts at: 2092 2599 2092
Size: 507 5435
SfoI GGC'GCC
Cuts at: 3374 3622 3374
Size: 248 5694
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5942
SmaI CCC'GGG
Cuts at: 115 838 1598 2964 115
Size: 723 760 1366 3093
Fragments arranged by size:
3093 1366 760 723
SmlI C'TyrA_G
Cuts at: 3097 4012 4504 4766 3097
Size: 915 492 262 4273
Fragments arranged by size:
4273 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5942
SphI G_CATG'C
Cuts at: 1011 2995 3495 1011
Size: 1984 500 3458
Fragments arranged by size:
3458 1984 500
StyI C'CwwG_G
Cuts at: 107 330 1739 2537 2753 3076 3430 107
Size: 223 1409 798 216 323 354 2619
Fragments arranged by size:
2619 1409 798 354 323 223 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 858 2256 3525 3658 4117 4326 858
Size: 1398 1269 133 459 209 2474
Fragments arranged by size:
2474 1398 1269 459 209 133
TatI w'GTAC_w
Cuts at: 908 1322 5457 908
Size: 414 4135 1393
Fragments arranged by size:
4135 1393 414
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 189 221 1141 1173 5459 5491 189
Size: 32 920 32 4286 32 640
Fragments arranged by size:
4286 920 640 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3739 3739
Size: 5942
XbaI T'CTAG_A
Cuts at: 2973 2973
Size: 5942
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1458 1935 2205 2701 2751 3437 1458
Size: 477 270 496 50 686 3963
Fragments arranged by size:
3963 686 496 477 270 50
XmaI C'CCGG_G
Cuts at: 113 836 1596 2962 113
Size: 723 760 1366 3093
Fragments arranged by size:
3093 1366 760 723
XmnI GAAnn'nnTTC
Cuts at: 2299 2299
Size: 5942
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BsmI BsmBI BstZ17I Bsu36I
ClaI CspCI EcoRV FseI HindIII HpaI MfeI NdeI
NheI NruI NsiI PacI PasI PshAI PsiI PspXI
SanDI SexAI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI