Restriction map for
pF1KE3281
(Circular) (Six-base) MAPSORT of: pF1KE3281.seq Check: 1222 from: 1 to: 5249
pF1KE3281 5249 bp
With 182 enzymes: SgfI *
March 4, 2011 18:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 954 954
Size: 5249
AccI GT'mk_AC
Cuts at: 2287 2287
Size: 5249
Acc65I G'GTAC_C
Cuts at: 2265 2265
Size: 5249
AclI AA'CG_TT
Cuts at: 5089 5089
Size: 5249
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1431 1615 2009 3074 3506 4253 4521 1431
Size: 184 394 1065 432 747 268 2159
Fragments arranged by size:
2159 1065 747 432 394 268 184
AflIII A'CryG_T
Cuts at: 2601 3705 4682 2601
Size: 1104 977 3168
Fragments arranged by size:
3168 1104 977
AgeI A'CCGG_T
Cuts at: 987 3637 987
Size: 2650 2599
AleI CACnn'nnGTG
Cuts at: 2170 2170
Size: 5249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1406 1406
Size: 5249
AlwNI CAG_nnn'CTG
Cuts at: 349 2554 4121 349
Size: 2205 1567 1477
Fragments arranged by size:
2205 1567 1477
ApaLI G'TGCA_C
Cuts at: 4019 4019
Size: 5249
ApoI r'AATT_y
Cuts at: 363 393 753 1088 1143 1572 2253 4602 4858
Size: 30 360 335 55 429 681 2349 256
Cuts at: 4858 363
Size: 754
Fragments arranged by size:
2349 754 681 429 360 335 256 55
30
AseI AT'TA_AT
Cuts at: 20 1366 1625 20
Size: 1346 259 3644
Fragments arranged by size:
3644 1346 259
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5249
AvaI C'yCGr_G
Cuts at: 320 767 2269 320
Size: 447 1502 3300
Fragments arranged by size:
3300 1502 447
AvrII C'CTAG_G
Cuts at: 1237 1237
Size: 5249
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 764 797 764
Size: 33 5216
BamHI G'GATC_C
Cuts at: 540 2274 540
Size: 1734 3515
BanI G'GyrC_C
Cuts at: 206 763 2265 2679 2927 2962 206
Size: 557 1502 414 248 35 2493
Fragments arranged by size:
2493 1502 557 414 248 35
BanII G_rGCy'C
Cuts at: 293 321 489 738 950 1237 1938 2263 3293
Size: 28 168 249 212 287 701 325 1030
Cuts at: 3293 293
Size: 2249
Fragments arranged by size:
2249 1030 701 325 287 249 212 168
28
BbeI G_GCGC'C
Cuts at: 2683 2931 2683
Size: 248 5001
BbsI GAAGACnn'nnnn_
Cuts at: 139 1232 139
Size: 1093 4156
BbvCI CC'TCA_GC
Cuts at: 410 410
Size: 5249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5215
BciVI GTATCCnnnnn_n'
Cuts at: 977 1572 3147 3908 5249 977
Size: 595 1575 761 1341 977
Fragments arranged by size:
1575 1341 977 761 595
BclI T'GATC_A
Cuts at: 114 1155 2769 114
Size: 1041 1614 2594
Fragments arranged by size:
2594 1614 1041
BglI GCCn_nnn'nGGC
Cuts at: 301 301
Size: 5249
BglII A'GATC_T
Cuts at: 189 431 1505 2070 2764 4801 189
Size: 242 1074 565 694 2037 637
Fragments arranged by size:
2037 1074 694 637 565 242
BlpI GC'TnA_GC
Cuts at: 2025 2361 2025
Size: 336 4913
Bme1580I G_kGCm'C
Cuts at: 267 768 2874 2967 4023 267
Size: 501 2106 93 1056 1493
Fragments arranged by size:
2106 1493 1056 501 93
BmgBI CAC'GTC
Cuts at: 2168 2168
Size: 5249
BmrI ACTGGGnnnn_n'
Cuts at: 2461 2542 2877 5021 2461
Size: 81 335 2144 2689
Fragments arranged by size:
2689 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3616 3616
Size: 5249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1053 1053
Size: 5249
Bpu10I CC'TnA_GC
Cuts at: 410 410
Size: 5249
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1251 1455 2425 3304 3796 4094 1251
Size: 204 970 879 492 298 2406
Fragments arranged by size:
2406 970 879 492 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 1069 4953 1069
Size: 3884 1365
BsaAI yAC'GTr
Cuts at: 1534 2602 1534
Size: 1068 4181
BsaBI GATnn'nnATC
Cuts at: 6 2763 4800 6
Size: 2757 2037 455
Fragments arranged by size:
2757 2037 455
BsaHI Gr'CG_yC
Cuts at: 2680 2928 3630 5126 2680
Size: 248 702 1496 2803
Fragments arranged by size:
2803 1496 702 248
BsaWI w'CCGG_w
Cuts at: 987 2438 2959 3637 3911 4058 4469 987
Size: 1451 521 678 274 147 411 1767
Fragments arranged by size:
1767 1451 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 977 1007 977
Size: 30 5219
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 985 1018 1152 985
Size: 33 134 5082
Fragments arranged by size:
5082 134 33
BseYI C'CCAG_C
Cuts at: 1770 2674 3432 4009 4505 1770
Size: 904 758 577 496 2514
Fragments arranged by size:
2514 904 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 962 2221 962
Size: 1259 3990
BsiEI CG_ry'CG
Cuts at: 104 2791 2837 4045 104
Size: 2687 46 1208 1308
Fragments arranged by size:
2687 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 321 1307 2263 3041 3231 4023 321
Size: 986 956 778 190 792 1547
Fragments arranged by size:
1547 986 956 792 778 190
BsmI GAATG_Cn'
Cuts at: 1395 1395
Size: 5249
Bsp1286I G_dGCh'C
Cuts at: 267 293 321 489 738 768 950 1237 1307
Size: 26 28 168 249 30 182 287 70
Cuts at: 1307 1938 2263 2874 2967 3041 3231 3293 4023
Size: 631 325 611 93 74 190 62 730
Cuts at: 4023 267
Size: 1493
Fragments arranged by size:
1493 730 631 611 325 287 249 190
182 168 93 74 70 62 30 28
26
BspEI T'CCGG_A
Cuts at: 4469 4469
Size: 5249
BspHI T'CATG_A
Cuts at: 4425 4425
Size: 5249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 954 2299 2513 2815 3196 954
Size: 1345 214 302 381 3007
Fragments arranged by size:
3007 1345 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3541 3595 5079 5244 50
Size: 3491 54 1484 165 55
Fragments arranged by size:
3491 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1751 3161 1751
Size: 1410 3839
BsrFI r'CCGG_y
Cuts at: 987 3247 3428 3637 987
Size: 2260 181 209 2599
Fragments arranged by size:
2599 2260 209 181
BsrGI T'GTAC_A
Cuts at: 1907 1907
Size: 5249
BssSI C'ACGA_G
Cuts at: 3520 3878 3520
Size: 358 4891
BstAPI GCAn_nnn'nTGC
Cuts at: 1192 2626 1192
Size: 1434 3815
BstBI TT'CG_AA
Cuts at: 3610 3610
Size: 5249
BstEII G'GTnAC_C
Cuts at: 720 720
Size: 5249
BstYI r'GATC_y
Cuts at: 1 189 431 540 1505 2070 2274 2741 2764
Size: 188 242 109 965 565 204 467 23
Cuts at: 2764 3099 3345 4357 4443 4455 4801 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 965 565 467 449 346 335 246
242 204 188 109 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3199 3380 3543 3199
Size: 181 163 4905
Fragments arranged by size:
4905 181 163
BtsI GCAGTG_nn'
Cuts at: 2492 2492
Size: 5249
DraI TTT'AAA
Cuts at: 1476 2248 1476
Size: 772 4477
DraIII CAC_nnn'GTG
Cuts at: 651 1329 651
Size: 678 4571
DrdI GACnn_nn'nnGTC
Cuts at: 661 2955 3813 661
Size: 2294 858 2097
Fragments arranged by size:
2294 2097 858
EaeI y'GGCC_r
Cuts at: 2237 2834 3008 3399 3426 4771 2237
Size: 597 174 391 27 1345 2715
Fragments arranged by size:
2715 1345 597 391 174 27
EagI C'GGCC_G
Cuts at: 2834 2834
Size: 5249
EarI CTCTTCn'nnn_
Cuts at: 632 1450 2036 2047 3272 3482 632
Size: 818 586 11 1225 210 2399
Fragments arranged by size:
2399 1225 818 586 210 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3457 3767 3913 5059 3457
Size: 310 146 1146 3647
Fragments arranged by size:
3647 1146 310 146
EcoICRI GAG'CTC
Cuts at: 319 2261 319
Size: 1942 3307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1053 1431 1615 2009 3074 3506 4253 4521 1053
Size: 378 184 394 1065 432 747 268 1781
Fragments arranged by size:
1781 1065 747 432 394 378 268 184
EcoO109I rG'GnC_Cy
Cuts at: 1068 2388 1068
Size: 1320 3929
EcoRI G'AATT_C
Cuts at: 2253 2253
Size: 5249
EcoRV GAT'ATC
Cuts at: 1539 1539
Size: 5249
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1709 1867 3268 1709
Size: 158 1401 3690
Fragments arranged by size:
3690 1401 158
FspI TGC'GCA
Cuts at: 3030 3030
Size: 5249
HaeII r_GCGC'y
Cuts at: 2683 2931 3953 4932 2683
Size: 248 1022 979 3000
Fragments arranged by size:
3000 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 500 532 704 736 1147 1179 3091 3121 3123
Size: 32 172 32 411 32 1912 30 2
Cuts at: 3123 3153 500
Size: 30 2596
Fragments arranged by size:
2596 1912 411 172 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 666 2288 666
Size: 1622 3627
KasI G'GCGC_C
Cuts at: 2679 2927 2679
Size: 248 5001
KpnI G_GTAC'C
Cuts at: 2269 2269
Size: 5249
MluI A'CGCG_T
Cuts at: 4682 4682
Size: 5249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2157 3920 4104 2157
Size: 1763 184 3302
Fragments arranged by size:
3302 1763 184
MscI TGG'CCA
Cuts at: 2239 3010 2239
Size: 771 4478
MslI CAynn'nnrTG
Cuts at: 890 1915 2170 3238 3365 890
Size: 1025 255 1068 127 2774
Fragments arranged by size:
2774 1068 1025 255 127
MspA1I CmG'CkG
Cuts at: 919 2019 2360 2674 3034 4047 4292 919
Size: 1100 341 314 360 1013 245 1876
Fragments arranged by size:
1876 1100 1013 360 341 314 245
NaeI GCC'GGC
Cuts at: 3430 3430
Size: 5249
NarI GG'CG_CC
Cuts at: 2680 2928 2680
Size: 248 5001
NgoMIV G'CCGG_C
Cuts at: 3428 3428
Size: 5249
NspI r_CATG'y
Cuts at: 2302 2802 3709 2302
Size: 500 907 3842
Fragments arranged by size:
3842 907 500
PciI A'CATG_T
Cuts at: 3705 3705
Size: 5249
PflMI CCAn_nnn'nTGG
Cuts at: 570 1329 1777 570
Size: 759 448 4042
Fragments arranged by size:
4042 759 448
PmeI GTTT'AAAC
Cuts at: 2248 2248
Size: 5249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 4388
Size: 32 5217
PpuMI rG'GwC_Cy
Cuts at: 1068 1068
Size: 5249
PstI C_TGCA'G
Cuts at: 699 1197 2296 2981 699
Size: 498 1099 685 2967
Fragments arranged by size:
2967 1099 685 498
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5249
PvuII CAG'CTG
Cuts at: 2019 2674 3034 2019
Size: 655 360 4234
Fragments arranged by size:
4234 655 360
RsrII CG'GwC_CG
Cuts at: 3444 3444
Size: 5249
SacI G_AGCT'C
Cuts at: 321 2263 321
Size: 1942 3307
SalI G'TCGA_C
Cuts at: 2286 2286
Size: 5249
SapI GCTCTTCn'nnn_
Cuts at: 632 2036 3272 3482 632
Size: 1404 1236 210 2399
Fragments arranged by size:
2399 1404 1236 210
SbfI CC_TGCA'GG
Cuts at: 2296 2296
Size: 5249
ScaI AGT'ACT
Cuts at: 4766 4766
Size: 5249
SexAI A'CCwGG_T
Cuts at: 1325 1325
Size: 5249
SfcI C'TryA_G
Cuts at: 33 695 1193 2292 2977 3970 4161 33
Size: 662 498 1099 685 993 191 1121
Fragments arranged by size:
1121 1099 993 685 662 498 191
SfoI GGC'GCC
Cuts at: 2681 2929 2681
Size: 248 5001
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5249
SmaI CCC'GGG
Cuts at: 2271 2271
Size: 5249
SmlI C'TyrA_G
Cuts at: 1230 1434 2404 3319 3811 4073 1230
Size: 204 970 915 492 262 2406
Fragments arranged by size:
2406 970 915 492 262 204
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5249
SphI G_CATG'C
Cuts at: 2302 2802 2302
Size: 500 4749
SspI AAT'ATT
Cuts at: 1614 1818 2009 1614
Size: 204 191 4854
Fragments arranged by size:
4854 204 191
StyI C'CwwG_G
Cuts at: 443 1237 2383 2737 443
Size: 794 1146 354 2955
Fragments arranged by size:
2955 1146 794 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 562 2832 2965 3424 3633 562
Size: 2270 133 459 209 2178
Fragments arranged by size:
2270 2178 459 209 133
TatI w'GTAC_w
Cuts at: 1907 4764 1907
Size: 2857 2392
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4766 4798 4766
Size: 32 5217
Tth111I GACn'n_nGTC
Cuts at: 282 3046 282
Size: 2764 2485
XbaI T'CTAG_A
Cuts at: 2280 2280
Size: 5249
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 571 2744 571
Size: 2173 3076
XmaI C'CCGG_G
Cuts at: 2269 2269
Size: 5249
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI BfrBI
BmtI BsiWI BsmBI BssHII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI