Restriction map for
pF1KE3275
(Circular) (Six-base) MAPSORT of: pF1KE3275.seq Check: 7922 from: 1 to: 5177
pF1KE3275 5177 bp
With 182 enzymes: SgfI *
July 27, 2017 10:26 ..
AccI GT'mk_AC
Cuts at: 423 1049 1490 2215 423
Size: 626 441 725 3385
Fragments arranged by size:
3385 725 626 441
Acc65I G'GTAC_C
Cuts at: 1162 2193 1162
Size: 1031 4146
AclI AA'CG_TT
Cuts at: 644 5017 644
Size: 4373 804
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1378 1390 3002 3434 4181 4449 1378
Size: 12 1612 432 747 268 2106
Fragments arranged by size:
2106 1612 747 432 268 12
AflIII A'CryG_T
Cuts at: 498 2529 3633 4610 498
Size: 2031 1104 977 1065
Fragments arranged by size:
2031 1104 1065 977
AgeI A'CCGG_T
Cuts at: 3565 3565
Size: 5177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 783 783
Size: 5177
AlwNI CAG_nnn'CTG
Cuts at: 745 2122 2482 4049 745
Size: 1377 360 1567 1873
Fragments arranged by size:
1873 1567 1377 360
ApaLI G'TGCA_C
Cuts at: 3947 3947
Size: 5177
ApoI r'AATT_y
Cuts at: 428 563 598 1902 2181 4530 4786 428
Size: 135 35 1304 279 2349 256 819
Fragments arranged by size:
2349 1304 819 279 256 135 35
AseI AT'TA_AT
Cuts at: 20 335 20
Size: 315 4862
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5177
AvaI C'yCGr_G
Cuts at: 1183 1526 2197 1183
Size: 343 671 4163
Fragments arranged by size:
4163 671 343
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1486 1519 1486
Size: 33 5144
BamHI G'GATC_C
Cuts at: 2202 2202
Size: 5177
BanI G'GyrC_C
Cuts at: 1162 2193 2607 2855 2890 1162
Size: 1031 414 248 35 3449
Fragments arranged by size:
3449 1031 414 248 35
BanII G_rGCy'C
Cuts at: 218 2132 2191 3221 218
Size: 1914 59 1030 2174
Fragments arranged by size:
2174 1914 1030 59
BbeI G_GCGC'C
Cuts at: 2611 2859 2611
Size: 248 4929
BbsI GAAGACnn'nnnn_
Cuts at: 410 410
Size: 5177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 183 217 2151 2185 183
Size: 34 1934 34 3175
Fragments arranged by size:
3175 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 232 1575 3075 3836 5177 232
Size: 1343 1500 761 1341 232
Fragments arranged by size:
1500 1343 1341 761 232
BclI T'GATC_A
Cuts at: 2697 2697
Size: 5177
BfrBI ATG'CAT
Cuts at: 1964 1964
Size: 5177
BglII A'GATC_T
Cuts at: 1110 2692 4729 1110
Size: 1582 2037 1558
Fragments arranged by size:
2037 1582 1558
BlpI GC'TnA_GC
Cuts at: 2289 2289
Size: 5177
Bme1580I G_kGCm'C
Cuts at: 2091 2802 2895 3951 2091
Size: 711 93 1056 3317
Fragments arranged by size:
3317 1056 711 93
BmrI ACTGGGnnnn_n'
Cuts at: 212 1819 2389 2470 2805 4949 212
Size: 1607 570 81 335 2144 440
Fragments arranged by size:
2144 1607 570 440 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3544 3544
Size: 5177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 139
Size: 5177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2353 3232 3724 4022 2353
Size: 879 492 298 3508
Fragments arranged by size:
3508 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4881 4881
Size: 5177
BsaAI yAC'GTr
Cuts at: 501 2530 501
Size: 2029 3148
BsaBI GATnn'nnATC
Cuts at: 6 2691 4728 6
Size: 2685 2037 455
Fragments arranged by size:
2685 2037 455
BsaHI Gr'CG_yC
Cuts at: 2608 2856 3558 5054 2608
Size: 248 702 1496 2731
Fragments arranged by size:
2731 1496 702 248
BsaWI w'CCGG_w
Cuts at: 224 2366 2887 3565 3839 3986 4397 224
Size: 2142 521 678 274 147 411 1004
Fragments arranged by size:
2142 1004 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 976 1006 1216 1246 1344 1374 976
Size: 30 210 30 98 30 4779
Fragments arranged by size:
4779 210 98 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 719 1895 719
Size: 1176 4001
BseYI C'CCAG_C
Cuts at: 1416 2069 2602 3360 3937 4433 1416
Size: 653 533 758 577 496 2160
Fragments arranged by size:
2160 758 653 577 533 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2050 2050
Size: 5177
BsiEI CG_ry'CG
Cuts at: 104 182 2719 2765 3973 104
Size: 78 2537 46 1208 1308
Fragments arranged by size:
2537 1308 1208 78 46
BsiHKAI G_wGCw'C
Cuts at: 1457 2132 2191 2969 3159 3951 1457
Size: 675 59 778 190 792 2683
Fragments arranged by size:
2683 792 778 675 190 59
BsmBI CGTCTCn'nnnn_
Cuts at: 1394 1394
Size: 5177
Bsp1286I G_dGCh'C
Cuts at: 218 1457 2091 2132 2191 2802 2895 2969 3159
Size: 1239 634 41 59 611 93 74 190
Cuts at: 3159 3221 3951 218
Size: 62 730 1444
Fragments arranged by size:
1444 1239 730 634 611 190 93 74
62 59 41
BspEI T'CCGG_A
Cuts at: 224 4397 224
Size: 4173 1004
BspHI T'CATG_A
Cuts at: 446 4353 446
Size: 3907 1270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2227 2441 2743 3124 2227
Size: 214 302 381 4280
Fragments arranged by size:
4280 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3469 3523 5007 5172 50
Size: 3419 54 1484 165 55
Fragments arranged by size:
3419 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 767 2011 3089 767
Size: 1244 1078 2855
Fragments arranged by size:
2855 1244 1078
BsrFI r'CCGG_y
Cuts at: 3175 3356 3565 3175
Size: 181 209 4787
Fragments arranged by size:
4787 209 181
BssSI C'ACGA_G
Cuts at: 3448 3806 3448
Size: 358 4819
BstAPI GCAn_nnn'nTGC
Cuts at: 1696 2554 1696
Size: 858 4319
BstBI TT'CG_AA
Cuts at: 3538 3538
Size: 5177
BstYI r'GATC_y
Cuts at: 1 921 1110 2202 2669 2692 3027 3273 4285
Size: 920 189 1092 467 23 335 246 1012
Cuts at: 4285 4371 4383 4729 1
Size: 86 12 346 449
Fragments arranged by size:
1092 1012 920 467 449 346 335 246
189 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 350 350
Size: 5177
BtgI C'CryG_G
Cuts at: 107 155 1167 107
Size: 48 1012 4117
Fragments arranged by size:
4117 1012 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3127 3308 3471 3127
Size: 181 163 4833
Fragments arranged by size:
4833 181 163
BtsI GCAGTG_nn'
Cuts at: 1224 2038 2420 1224
Size: 814 382 3981
Fragments arranged by size:
3981 814 382
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1496 1531 1496
Size: 35 5142
DraI TTT'AAA
Cuts at: 312 2176 312
Size: 1864 3313
DraIII CAC_nnn'GTG
Cuts at: 969 969
Size: 5177
DrdI GACnn_nn'nnGTC
Cuts at: 2883 3741 2883
Size: 858 4319
EaeI y'GGCC_r
Cuts at: 131 152 179 1749 2762 2936 3327 3354 4699
Size: 21 27 1570 1013 174 391 27 1345
Cuts at: 4699 131
Size: 609
Fragments arranged by size:
1570 1345 1013 609 391 174 27 27
21
EagI C'GGCC_G
Cuts at: 179 2762 179
Size: 2583 2594
EarI CTCTTCn'nnn_
Cuts at: 157 362 775 1571 1720 3200 3410 157
Size: 205 413 796 149 1480 210 1924
Fragments arranged by size:
1924 1480 796 413 210 205 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 3385 3695 3841 4987 127
Size: 3258 310 146 1146 317
Fragments arranged by size:
3258 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 2130 2189 2130
Size: 59 5118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 1378 1390 3002 3434 4181 4449 139
Size: 1239 12 1612 432 747 268 867
Fragments arranged by size:
1612 1239 867 747 432 268 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1381 2114 1381
Size: 733 4444
EcoO109I rG'GnC_Cy
Cuts at: 2316 2316
Size: 5177
EcoRI G'AATT_C
Cuts at: 2181 2181
Size: 5177
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 427 3196 427
Size: 2769 2408
FspI TGC'GCA
Cuts at: 2958 2958
Size: 5177
HaeII r_GCGC'y
Cuts at: 2611 2859 3881 4860 2611
Size: 248 1022 979 2928
Fragments arranged by size:
2928 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 270 302 522 554 1216 1248 1342 1374 1549
Size: 32 220 32 662 32 94 32 175
Cuts at: 1549 1581 1876 1908 3019 3049 3051 3081 270
Size: 32 295 32 1111 30 2 30 2366
Fragments arranged by size:
2366 1111 662 295 220 175 94 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 424 1476 2216 424
Size: 1052 740 3385
Fragments arranged by size:
3385 1052 740
KasI G'GCGC_C
Cuts at: 2607 2855 2607
Size: 248 4929
KpnI G_GTAC'C
Cuts at: 1166 2197 1166
Size: 1031 4146
MluI A'CGCG_T
Cuts at: 4610 4610
Size: 5177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3848 4032 3848
Size: 184 4993
MscI TGG'CCA
Cuts at: 154 1751 2938 154
Size: 1597 1187 2393
Fragments arranged by size:
2393 1597 1187
MslI CAynn'nnrTG
Cuts at: 1154 3166 3293 1154
Size: 2012 127 3038
Fragments arranged by size:
3038 2012 127
MspA1I CmG'CkG
Cuts at: 2073 2288 2602 2962 3975 4220 2073
Size: 215 314 360 1013 245 3030
Fragments arranged by size:
3030 1013 360 314 245 215
NaeI GCC'GGC
Cuts at: 3358 3358
Size: 5177
NarI GG'CG_CC
Cuts at: 2608 2856 2608
Size: 248 4929
NcoI C'CATG_G
Cuts at: 107 155 1167 107
Size: 48 1012 4117
Fragments arranged by size:
4117 1012 48
NgoMIV G'CCGG_C
Cuts at: 3356 3356
Size: 5177
NsiI A_TGCA'T
Cuts at: 1966 1966
Size: 5177
NspI r_CATG'y
Cuts at: 1032 1248 1489 1803 2230 2730 3637 1032
Size: 216 241 314 427 500 907 2572
Fragments arranged by size:
2572 907 500 427 314 241 216
PasI CC'CwG_GG
Cuts at: 1236 1236
Size: 5177
PciI A'CATG_T
Cuts at: 3633 3633
Size: 5177
PfoI T'CCnGG_A
Cuts at: 917 917
Size: 5177
PmeI GTTT'AAAC
Cuts at: 2176 2176
Size: 5177
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1910 1942 4316 4348 1910
Size: 32 2374 32 2739
Fragments arranged by size:
2739 2374 32 32
PstI C_TGCA'G
Cuts at: 1196 2224 2909 1196
Size: 1028 685 3464
Fragments arranged by size:
3464 1028 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5177
PvuII CAG'CTG
Cuts at: 2602 2962 2602
Size: 360 4817
RsrII CG'GwC_CG
Cuts at: 3372 3372
Size: 5177
SacI G_AGCT'C
Cuts at: 2132 2191 2132
Size: 59 5118
SalI G'TCGA_C
Cuts at: 422 2214 422
Size: 1792 3385
SapI GCTCTTCn'nnn_
Cuts at: 157 3200 3410 157
Size: 3043 210 1924
Fragments arranged by size:
3043 1924 210
SbfI CC_TGCA'GG
Cuts at: 2224 2224
Size: 5177
ScaI AGT'ACT
Cuts at: 4694 4694
Size: 5177
SfcI C'TryA_G
Cuts at: 33 491 1050 1192 2220 2905 3898 4089 33
Size: 458 559 142 1028 685 993 191 1121
Fragments arranged by size:
1121 1028 993 685 559 458 191 142
SfoI GGC'GCC
Cuts at: 2609 2857 2609
Size: 248 4929
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5177
SmaI CCC'GGG
Cuts at: 1528 2199 1528
Size: 671 4506
SmlI C'TyrA_G
Cuts at: 2332 3247 3739 4001 2332
Size: 915 492 262 3508
Fragments arranged by size:
3508 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5177
SphI G_CATG'C
Cuts at: 1248 2230 2730 1248
Size: 982 500 3695
Fragments arranged by size:
3695 982 500
SspI AAT'ATT
Cuts at: 1074 1074
Size: 5177
StyI C'CwwG_G
Cuts at: 107 155 1167 2311 2665 107
Size: 48 1012 1144 354 2619
Fragments arranged by size:
2619 1144 1012 354 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1331 2102 2760 2893 3352 3561 1331
Size: 771 658 133 459 209 2947
Fragments arranged by size:
2947 771 658 459 209 133
TatI w'GTAC_w
Cuts at: 4692 4692
Size: 5177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1344 1376 4694 4726 1344
Size: 32 3318 32 1795
Fragments arranged by size:
3318 1795 32 32
Tth111I GACn'n_nGTC
Cuts at: 2974 2974
Size: 5177
XbaI T'CTAG_A
Cuts at: 2208 2208
Size: 5177
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2672 114
Size: 2558 2619
XmaI C'CCGG_G
Cuts at: 1526 2197 1526
Size: 671 4506
XmnI GAAnn'nnTTC
Cuts at: 575 676 1010 1922 575
Size: 101 334 912 3830
Fragments arranged by size:
3830 912 334 101
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BbvCI BglI BmgBI BmtI Bpu10I BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI