Restriction map for
pF1KE3190
(Circular) (Six-base) MAPSORT of: pF1KE3190.seq Check: 8548 from: 1 to: 4439
pF1KE3190 4439 bp
With 182 enzymes: SgfI *
July 14, 2018 20:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 167 167
Size: 4439
AccI GT'mk_AC
Cuts at: 1477 1477
Size: 4439
Acc65I G'GTAC_C
Cuts at: 1455 1455
Size: 4439
AclI AA'CG_TT
Cuts at: 4279 4279
Size: 4439
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 1415 2264 2696 3443 3711 388
Size: 1027 849 432 747 268 1116
Fragments arranged by size:
1116 1027 849 747 432 268
AflIII A'CryG_T
Cuts at: 1331 1791 2895 3872 1331
Size: 460 1104 977 1898
Fragments arranged by size:
1898 1104 977 460
AgeI A'CCGG_T
Cuts at: 2827 2827
Size: 4439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 152 175 337 152
Size: 23 162 4254
Fragments arranged by size:
4254 162 23
AlwNI CAG_nnn'CTG
Cuts at: 1393 1744 3311 1393
Size: 351 1567 2521
Fragments arranged by size:
2521 1567 351
ApaLI G'TGCA_C
Cuts at: 3209 3209
Size: 4439
ApoI r'AATT_y
Cuts at: 249 304 441 656 992 1345 1443 3792 4048
Size: 55 137 215 336 353 98 2349 256
Cuts at: 4048 249
Size: 640
Fragments arranged by size:
2349 640 353 336 256 215 137 98
55
AseI AT'TA_AT
Cuts at: 20 20
Size: 4439
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
AvaI C'yCGr_G
Cuts at: 1459 1459
Size: 4439
AvrII C'CTAG_G
Cuts at: 285 285
Size: 4439
BamHI G'GATC_C
Cuts at: 1464 1464
Size: 4439
BanI G'GyrC_C
Cuts at: 1455 1869 2117 2152 1455
Size: 414 248 35 3742
Fragments arranged by size:
3742 414 248 35
BanII G_rGCy'C
Cuts at: 1453 2483 1453
Size: 1030 3409
BbeI G_GCGC'C
Cuts at: 1873 2121 1873
Size: 248 4191
BbsI GAAGACnn'nnnn_
Cuts at: 215 597 761 961 215
Size: 382 164 200 3693
Fragments arranged by size:
3693 382 200 164
BciVI GTATCCnnnnn_n'
Cuts at: 2337 3098 4439 2337
Size: 761 1341 2337
Fragments arranged by size:
2337 1341 761
BclI T'GATC_A
Cuts at: 1959 1959
Size: 4439
BglI GCCn_nnn'nGGC
Cuts at: 834 834
Size: 4439
BglII A'GATC_T
Cuts at: 1283 1954 3991 1283
Size: 671 2037 1731
Fragments arranged by size:
2037 1731 671
BlpI GC'TnA_GC
Cuts at: 1551 1551
Size: 4439
Bme1580I G_kGCm'C
Cuts at: 2064 2157 3213 2064
Size: 93 1056 3290
Fragments arranged by size:
3290 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1269 1651 1732 2067 4211 1269
Size: 382 81 335 2144 1497
Fragments arranged by size:
2144 1497 382 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2806 2806
Size: 4439
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 631 732 1615 2494 2986 3284 404
Size: 227 101 883 879 492 298 1559
Fragments arranged by size:
1559 883 879 492 298 227 101
BsaI GGTCTCn'nnnn_
Cuts at: 853 4143 853
Size: 3290 1149
BsaAI yAC'GTr
Cuts at: 892 1792 892
Size: 900 3539
BsaBI GATnn'nnATC
Cuts at: 6 373 905 1953 3990 6
Size: 367 532 1048 2037 455
Fragments arranged by size:
2037 1048 532 455 367
BsaHI Gr'CG_yC
Cuts at: 1870 2118 2820 4316 1870
Size: 248 702 1496 1993
Fragments arranged by size:
1993 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1628 2149 2827 3101 3248 3659 1628
Size: 521 678 274 147 411 2408
Fragments arranged by size:
2408 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1864 2622 3199 3695 1864
Size: 758 577 496 2608
Fragments arranged by size:
2608 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1981 2027 3235 104
Size: 1877 46 1208 1308
Fragments arranged by size:
1877 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1453 2231 2421 3213 1453
Size: 778 190 792 2679
Fragments arranged by size:
2679 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1453 2064 2157 2231 2421 2483 3213 1453
Size: 611 93 74 190 62 730 2679
Fragments arranged by size:
2679 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3659 3659
Size: 4439
BspHI T'CATG_A
Cuts at: 3615 3615
Size: 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 167 1489 1703 2005 2386 167
Size: 1322 214 302 381 2220
Fragments arranged by size:
2220 1322 381 302 214
BsrBI CCG'CTC
Cuts at: 50 165 2731 2785 4269 4434 50
Size: 115 2566 54 1484 165 55
Fragments arranged by size:
2566 1484 165 115 55 54
BsrDI GCAATG_nn'
Cuts at: 492 2351 492
Size: 1859 2580
BsrFI r'CCGG_y
Cuts at: 112 1223 2437 2618 2827 112
Size: 1111 1214 181 209 1724
Fragments arranged by size:
1724 1214 1111 209 181
BsrGI T'GTAC_A
Cuts at: 489 489
Size: 4439
BssSI C'ACGA_G
Cuts at: 2710 3068 2710
Size: 358 4081
BstAPI GCAn_nnn'nTGC
Cuts at: 1816 1816
Size: 4439
BstBI TT'CG_AA
Cuts at: 2800 2800
Size: 4439
BstYI r'GATC_y
Cuts at: 1 1283 1464 1931 1954 2289 2535 3547 3633
Size: 1282 181 467 23 335 246 1012 86
Cuts at: 3633 3645 3991 1
Size: 12 346 449
Fragments arranged by size:
1282 1012 467 449 346 335 246 181
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4439
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2389 2570 2733 2389
Size: 181 163 4095
Fragments arranged by size:
4095 181 163
BtsI GCAGTG_nn'
Cuts at: 1020 1682 1020
Size: 662 3777
DraI TTT'AAA
Cuts at: 1438 1438
Size: 4439
DrdI GACnn_nn'nnGTC
Cuts at: 2145 3003 2145
Size: 858 3581
EaeI y'GGCC_r
Cuts at: 110 1316 2024 2198 2589 2616 3961 110
Size: 1206 708 174 391 27 1345 588
Fragments arranged by size:
1345 1206 708 588 391 174 27
EagI C'GGCC_G
Cuts at: 2024 2024
Size: 4439
EarI CTCTTCn'nnn_
Cuts at: 407 787 929 2462 2672 407
Size: 380 142 1533 210 2174
Fragments arranged by size:
2174 1533 380 210 142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2647 2957 3103 4249 2647
Size: 310 146 1146 2837
Fragments arranged by size:
2837 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1451 1451
Size: 4439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 1415 2264 2696 3443 3711 388
Size: 1027 849 432 747 268 1116
Fragments arranged by size:
1116 1027 849 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1578 1578
Size: 4439
EcoRI G'AATT_C
Cuts at: 992 1443 992
Size: 451 3988
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1441 2458 1441
Size: 1017 3422
FseI GG_CCGG'CC
Cuts at: 1227 1227
Size: 4439
FspI TGC'GCA
Cuts at: 140 2220 140
Size: 2080 2359
HaeII r_GCGC'y
Cuts at: 1873 2121 3143 4122 1873
Size: 248 1022 979 2190
Fragments arranged by size:
2190 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 384 416 2281 2311 2313 2343 384
Size: 32 1865 30 2 30 2480
Fragments arranged by size:
2480 1865 32 30 30 2
HincII GTy'rAC
Cuts at: 1478 1478
Size: 4439
KasI G'GCGC_C
Cuts at: 1869 2117 1869
Size: 248 4191
KpnI G_GTAC'C
Cuts at: 1459 1459
Size: 4439
MfeI C'AATT_G
Cuts at: 584 584
Size: 4439
MluI A'CGCG_T
Cuts at: 3872 3872
Size: 4439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3110 3294 3110
Size: 184 4255
MscI TGG'CCA
Cuts at: 2200 2200
Size: 4439
MslI CAynn'nnrTG
Cuts at: 771 1169 2428 2555 771
Size: 398 1259 127 2655
Fragments arranged by size:
2655 1259 398 127
MspA1I CmG'CkG
Cuts at: 338 1244 1550 1864 2224 3237 3482 338
Size: 906 306 314 360 1013 245 1295
Fragments arranged by size:
1295 1013 906 360 314 306 245
NaeI GCC'GGC
Cuts at: 114 1225 2620 114
Size: 1111 1395 1933
Fragments arranged by size:
1933 1395 1111
NarI GG'CG_CC
Cuts at: 1870 2118 1870
Size: 248 4191
NcoI C'CATG_G
Cuts at: 107 107
Size: 4439
NgoMIV G'CCGG_C
Cuts at: 112 1223 2618 112
Size: 1111 1395 1933
Fragments arranged by size:
1933 1395 1111
NspI r_CATG'y
Cuts at: 1335 1492 1992 2899 1335
Size: 157 500 907 2875
Fragments arranged by size:
2875 907 500 157
PciI A'CATG_T
Cuts at: 1331 2895 1331
Size: 1564 2875
PfoI T'CCnGG_A
Cuts at: 384 384
Size: 4439
PmeI GTTT'AAAC
Cuts at: 1438 1438
Size: 4439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 3578
Size: 32 4407
PshAI GACnn'nnGTC
Cuts at: 260 338 260
Size: 78 4361
PstI C_TGCA'G
Cuts at: 177 696 1486 2171 177
Size: 519 790 685 2445
Fragments arranged by size:
2445 790 685 519
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4439
PvuII CAG'CTG
Cuts at: 338 1244 1864 2224 338
Size: 906 620 360 2553
Fragments arranged by size:
2553 906 620 360
RsrII CG'GwC_CG
Cuts at: 2634 2634
Size: 4439
SacI G_AGCT'C
Cuts at: 1453 1453
Size: 4439
SalI G'TCGA_C
Cuts at: 1476 1476
Size: 4439
SapI GCTCTTCn'nnn_
Cuts at: 2462 2672 2462
Size: 210 4229
SbfI CC_TGCA'GG
Cuts at: 1486 1486
Size: 4439
ScaI AGT'ACT
Cuts at: 3956 3956
Size: 4439
SfcI C'TryA_G
Cuts at: 33 173 692 1232 1482 2167 3160 3351 33
Size: 140 519 540 250 685 993 191 1121
Fragments arranged by size:
1121 993 685 540 519 250 191 140
SfoI GGC'GCC
Cuts at: 1871 2119 1871
Size: 248 4191
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
SmaI CCC'GGG
Cuts at: 1461 1461
Size: 4439
SmlI C'TyrA_G
Cuts at: 419 610 747 1594 2509 3001 3263 419
Size: 191 137 847 915 492 262 1595
Fragments arranged by size:
1595 915 847 492 262 191 137
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4439
SphI G_CATG'C
Cuts at: 1492 1992 1492
Size: 500 3939
SspI AAT'ATT
Cuts at: 1118 1118
Size: 4439
StyI C'CwwG_G
Cuts at: 107 285 1573 1927 107
Size: 178 1288 354 2619
Fragments arranged by size:
2619 1288 354 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2022 2155 2614 2823 2022
Size: 133 459 209 3638
Fragments arranged by size:
3638 459 209 133
TatI w'GTAC_w
Cuts at: 489 1060 3954 489
Size: 571 2894 974
Fragments arranged by size:
2894 974 571
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3956 3988 3956
Size: 32 4407
Tth111I GACn'n_nGTC
Cuts at: 2236 2236
Size: 4439
XbaI T'CTAG_A
Cuts at: 1470 1470
Size: 4439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1934 1934
Size: 4439
XmaI C'CCGG_G
Cuts at: 1459 1459
Size: 4439
XmnI GAAnn'nnTTC
Cuts at: 305 305
Size: 4439
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI Bpu10I
BsaXI BseRI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI