Restriction map for
pF1KE3148
(Circular) (Six-base) MAPSORT of: pF1KE3148.seq Check: 6593 from: 1 to: 4199
pF1KE3148 4199 bp
With 182 enzymes: SgfI *
November 18, 2016 17:23 ..
AccI GT'mk_AC
Cuts at: 137 1237 137
Size: 1100 3099
Acc65I G'GTAC_C
Cuts at: 1215 1215
Size: 4199
AclI AA'CG_TT
Cuts at: 4039 4039
Size: 4199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 2024 2456 3203 3471 1075
Size: 949 432 747 268 1803
Fragments arranged by size:
1803 949 747 432 268
AflII C'TTAA_G
Cuts at: 540 540
Size: 4199
AflIII A'CryG_T
Cuts at: 1551 2655 3632 1551
Size: 1104 977 2118
Fragments arranged by size:
2118 1104 977
AgeI A'CCGG_T
Cuts at: 744 2587 744
Size: 1843 2356
AlwNI CAG_nnn'CTG
Cuts at: 313 696 1504 3071 313
Size: 383 808 1567 1441
Fragments arranged by size:
1567 1441 808 383
ApaLI G'TGCA_C
Cuts at: 2969 2969
Size: 4199
ApoI r'AATT_y
Cuts at: 274 780 872 1131 1203 3552 3808 274
Size: 506 92 259 72 2349 256 665
Fragments arranged by size:
2349 665 506 259 256 92 72
AseI AT'TA_AT
Cuts at: 20 20
Size: 4199
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
AvaI C'yCGr_G
Cuts at: 1219 1219
Size: 4199
BamHI G'GATC_C
Cuts at: 1224 1224
Size: 4199
BanI G'GyrC_C
Cuts at: 250 805 1215 1629 1877 1912 250
Size: 555 410 414 248 35 2537
Fragments arranged by size:
2537 555 414 410 248 35
BanII G_rGCy'C
Cuts at: 147 467 1213 2243 147
Size: 320 746 1030 2103
Fragments arranged by size:
2103 1030 746 320
BbeI G_GCGC'C
Cuts at: 1633 1881 1633
Size: 248 3951
BciVI GTATCCnnnnn_n'
Cuts at: 544 2097 2858 4199 544
Size: 1553 761 1341 544
Fragments arranged by size:
1553 1341 761 544
BclI T'GATC_A
Cuts at: 1719 1719
Size: 4199
BglII A'GATC_T
Cuts at: 1714 3751 1714
Size: 2037 2162
BlpI GC'TnA_GC
Cuts at: 146 1311 146
Size: 1165 3034
Bme1580I G_kGCm'C
Cuts at: 1824 1917 2973 1824
Size: 93 1056 3050
Fragments arranged by size:
3050 1056 93
BmgBI CAC'GTC
Cuts at: 902 902
Size: 4199
BmrI ACTGGGnnnn_n'
Cuts at: 150 979 1411 1492 1827 3971 150
Size: 829 432 81 335 2144 378
Fragments arranged by size:
2144 829 432 378 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 4199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 304
Size: 4199
Bpu10I CC'TnA_GC
Cuts at: 473 473
Size: 4199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 1375 2254 2746 3044 224
Size: 1151 879 492 298 1379
Fragments arranged by size:
1379 1151 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1018 3903 1018
Size: 2885 1314
BsaAI yAC'GTr
Cuts at: 1552 1552
Size: 4199
BsaBI GATnn'nnATC
Cuts at: 6 1713 3750 6
Size: 1707 2037 455
Fragments arranged by size:
2037 1707 455
BsaHI Gr'CG_yC
Cuts at: 127 1630 1878 2580 4076 127
Size: 1503 248 702 1496 250
Fragments arranged by size:
1503 1496 702 250 248
BsaWI w'CCGG_w
Cuts at: 744 1027 1388 1909 2587 2861 3008 3419 744
Size: 283 361 521 678 274 147 411 1524
Fragments arranged by size:
1524 678 521 411 361 283 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 251 281 251
Size: 30 4169
BseYI C'CCAG_C
Cuts at: 1624 2382 2959 3455 1624
Size: 758 577 496 2368
Fragments arranged by size:
2368 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1741 1787 2995 104
Size: 1637 46 1208 1308
Fragments arranged by size:
1637 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 467 929 1213 1991 2181 2973 467
Size: 462 284 778 190 792 1693
Fragments arranged by size:
1693 792 778 462 284 190
Bsp1286I G_dGCh'C
Cuts at: 147 467 929 1213 1824 1917 1991 2181 2243
Size: 320 462 284 611 93 74 190 62
Cuts at: 2243 2973 147
Size: 730 1373
Fragments arranged by size:
1373 730 611 462 320 284 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3419 3419
Size: 4199
BspHI T'CATG_A
Cuts at: 3375 3375
Size: 4199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 327 1249 1463 1765 2146 327
Size: 922 214 302 381 2380
Fragments arranged by size:
2380 922 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2491 2545 4029 4194 50
Size: 2441 54 1484 165 55
Fragments arranged by size:
2441 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1045 2111 1045
Size: 1066 3133
BsrFI r'CCGG_y
Cuts at: 744 2197 2378 2587 744
Size: 1453 181 209 2356
Fragments arranged by size:
2356 1453 209 181
BssSI C'ACGA_G
Cuts at: 2470 2828 2470
Size: 358 3841
BstAPI GCAn_nnn'nTGC
Cuts at: 1576 1576
Size: 4199
BstBI TT'CG_AA
Cuts at: 2560 2560
Size: 4199
BstXI CCAn_nnnn'nTGG
Cuts at: 772 772
Size: 4199
BstYI r'GATC_y
Cuts at: 1 596 1187 1224 1691 1714 2049 2295 3307
Size: 595 591 37 467 23 335 246 1012
Cuts at: 3307 3393 3405 3751 1
Size: 86 12 346 449
Fragments arranged by size:
1012 595 591 467 449 346 335 246
86 37 23 12
BtgI C'CryG_G
Cuts at: 107 809 107
Size: 702 3497
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2149 2330 2493 2149
Size: 181 163 3855
Fragments arranged by size:
3855 181 163
BtsI GCAGTG_nn'
Cuts at: 1442 1442
Size: 4199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 395 430 395
Size: 35 4164
DraI TTT'AAA
Cuts at: 1198 1198
Size: 4199
DraIII CAC_nnn'GTG
Cuts at: 950 950
Size: 4199
DrdI GACnn_nn'nnGTC
Cuts at: 1905 2763 1905
Size: 858 3341
EaeI y'GGCC_r
Cuts at: 1784 1958 2349 2376 3721 1784
Size: 174 391 27 1345 2262
Fragments arranged by size:
2262 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1784 1784
Size: 4199
EarI CTCTTCn'nnn_
Cuts at: 476 2222 2432 476
Size: 1746 210 2243
Fragments arranged by size:
2243 1746 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 997 2407 2717 2863 4009 997
Size: 1410 310 146 1146 1187
Fragments arranged by size:
1410 1187 1146 310 146
EcoICRI GAG'CTC
Cuts at: 465 1211 465
Size: 746 3453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 1075 2024 2456 3203 3471 304
Size: 771 949 432 747 268 1032
Fragments arranged by size:
1032 949 771 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1338 1338
Size: 4199
EcoRI G'AATT_C
Cuts at: 1203 1203
Size: 4199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 4199
FspI TGC'GCA
Cuts at: 1980 1980
Size: 4199
HaeII r_GCGC'y
Cuts at: 489 1167 1633 1881 2903 3882 489
Size: 678 466 248 1022 979 806
Fragments arranged by size:
1022 979 806 678 466 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 251 283 611 643 2041 2071 2073 2103 251
Size: 32 328 32 1398 30 2 30 2347
Fragments arranged by size:
2347 1398 328 32 32 30 30 2
HincII GTy'rAC
Cuts at: 125 606 1238 125
Size: 481 632 3086
Fragments arranged by size:
3086 632 481
KasI G'GCGC_C
Cuts at: 1629 1877 1629
Size: 248 3951
KpnI G_GTAC'C
Cuts at: 1219 1219
Size: 4199
MfeI C'AATT_G
Cuts at: 725 866 725
Size: 141 4058
MluI A'CGCG_T
Cuts at: 3632 3632
Size: 4199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 525 2870 3054 525
Size: 2345 184 1670
Fragments arranged by size:
2345 1670 184
MscI TGG'CCA
Cuts at: 1960 1960
Size: 4199
MslI CAynn'nnrTG
Cuts at: 910 972 2188 2315 910
Size: 62 1216 127 2794
Fragments arranged by size:
2794 1216 127 62
MspA1I CmG'CkG
Cuts at: 1310 1624 1984 2997 3242 1310
Size: 314 360 1013 245 2267
Fragments arranged by size:
2267 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2380 2380
Size: 4199
NarI GG'CG_CC
Cuts at: 1630 1878 1630
Size: 248 3951
NcoI C'CATG_G
Cuts at: 107 809 107
Size: 702 3497
NgoMIV G'CCGG_C
Cuts at: 2378 2378
Size: 4199
NspI r_CATG'y
Cuts at: 136 897 1252 1752 2659 136
Size: 761 355 500 907 1676
Fragments arranged by size:
1676 907 761 500 355
PciI A'CATG_T
Cuts at: 2655 2655
Size: 4199
PflMI CCAn_nnn'nTGG
Cuts at: 666 696 666
Size: 30 4169
PfoI T'CCnGG_A
Cuts at: 592 592
Size: 4199
PmeI GTTT'AAAC
Cuts at: 1198 1198
Size: 4199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 3338
Size: 32 4167
PsiI TTA'TAA
Cuts at: 1105 1148 1105
Size: 43 4156
PstI C_TGCA'G
Cuts at: 1246 1931 1246
Size: 685 3514
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4199
PvuII CAG'CTG
Cuts at: 1624 1984 1624
Size: 360 3839
RsrII CG'GwC_CG
Cuts at: 2394 2394
Size: 4199
SacI G_AGCT'C
Cuts at: 467 1213 467
Size: 746 3453
SalI G'TCGA_C
Cuts at: 1236 1236
Size: 4199
SapI GCTCTTCn'nnn_
Cuts at: 476 2222 2432 476
Size: 1746 210 2243
Fragments arranged by size:
2243 1746 210
SbfI CC_TGCA'GG
Cuts at: 1246 1246
Size: 4199
ScaI AGT'ACT
Cuts at: 3716 3716
Size: 4199
SfcI C'TryA_G
Cuts at: 33 1242 1927 2920 3111 33
Size: 1209 685 993 191 1121
Fragments arranged by size:
1209 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1631 1879 1631
Size: 248 3951
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
SgrAI Cr'CCGG_yG
Cuts at: 744 744
Size: 4199
SmaI CCC'GGG
Cuts at: 1221 1221
Size: 4199
SmlI C'TyrA_G
Cuts at: 239 540 1354 2269 2761 3023 239
Size: 301 814 915 492 262 1415
Fragments arranged by size:
1415 915 814 492 301 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4199
SphI G_CATG'C
Cuts at: 1252 1752 1252
Size: 500 3699
SspI AAT'ATT
Cuts at: 1016 1016
Size: 4199
StyI C'CwwG_G
Cuts at: 107 809 1333 1687 107
Size: 702 524 354 2619
Fragments arranged by size:
2619 702 524 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1782 1915 2374 2583 1782
Size: 133 459 209 3398
Fragments arranged by size:
3398 459 209 133
TatI w'GTAC_w
Cuts at: 351 3714 351
Size: 3363 836
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3716 3748 3716
Size: 32 4167
Tth111I GACn'n_nGTC
Cuts at: 265 1996 265
Size: 1731 2468
XbaI T'CTAG_A
Cuts at: 1230 1230
Size: 4199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1694 1694
Size: 4199
XmaI C'CCGG_G
Cuts at: 1219 1219
Size: 4199
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI