Restriction map for
pF1KE3139
(Circular) (Six-base) MAPSORT of: pF1KE3139.seq Check: 8342 from: 1 to: 4163
pF1KE3139 4163 bp
With 182 enzymes: SgfI *
March 1, 2013 10:20 ..
AatII G_ACGT'C
Cuts at: 883 914 883
Size: 31 4132
AccI GT'mk_AC
Cuts at: 1201 1201
Size: 4163
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1988 2420 3167 3435 1988
Size: 432 747 268 2716
Fragments arranged by size:
2716 747 432 268
AfeI AGC'GCT
Cuts at: 709 709
Size: 4163
AflIII A'CryG_T
Cuts at: 323 1515 2619 3596 323
Size: 1192 1104 977 890
Fragments arranged by size:
1192 1104 977 890
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 343 343
Size: 4163
AlwNI CAG_nnn'CTG
Cuts at: 483 854 950 1468 3035 483
Size: 371 96 518 1567 1611
Fragments arranged by size:
1611 1567 518 371 96
ApaLI G'TGCA_C
Cuts at: 2933 2933
Size: 4163
ApoI r'AATT_y
Cuts at: 1093 1167 3516 3772 1093
Size: 74 2349 256 1484
Fragments arranged by size:
2349 1484 256 74
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 295 588 1018 1183 295
Size: 293 430 165 3275
Fragments arranged by size:
3275 430 293 165
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 483 516 483
Size: 33 4130
BamHI G'GATC_C
Cuts at: 728 1188 728
Size: 460 3703
BanI G'GyrC_C
Cuts at: 214 1179 1593 1841 1876 214
Size: 965 414 248 35 2501
Fragments arranged by size:
2501 965 414 248 35
BanII G_rGCy'C
Cuts at: 1025 1177 2207 1025
Size: 152 1030 2981
Fragments arranged by size:
2981 1030 152
BbeI G_GCGC'C
Cuts at: 1597 1845 1597
Size: 248 3915
BbsI GAAGACnn'nnnn_
Cuts at: 906 1092 906
Size: 186 3977
BbvCI CC'TCA_GC
Cuts at: 128 943 128
Size: 815 3348
BciVI GTATCCnnnnn_n'
Cuts at: 511 2061 2822 4163 511
Size: 1550 761 1341 511
Fragments arranged by size:
1550 1341 761 511
BclI T'GATC_A
Cuts at: 1683 1683
Size: 4163
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4163
BglII A'GATC_T
Cuts at: 1049 1678 3715 1049
Size: 629 2037 1497
Fragments arranged by size:
2037 1497 629
BlpI GC'TnA_GC
Cuts at: 775 1275 775
Size: 500 3663
Bme1580I G_kGCm'C
Cuts at: 448 602 1788 1881 2937 448
Size: 154 1186 93 1056 1674
Fragments arranged by size:
1674 1186 1056 154 93
BmgBI CAC'GTC
Cuts at: 615 1041 1067 615
Size: 426 26 3711
Fragments arranged by size:
3711 426 26
BmrI ACTGGGnnnn_n'
Cuts at: 1375 1456 1791 3935 1375
Size: 81 335 2144 1603
Fragments arranged by size:
2144 1603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 156 540 2530 156
Size: 384 1990 1789
Fragments arranged by size:
1990 1789 384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 592 126
Size: 466 3697
Bpu10I CC'TnA_GC
Cuts at: 128 943 128
Size: 815 3348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1339 2218 2710 3008 1339
Size: 879 492 298 2494
Fragments arranged by size:
2494 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 597 1516 597
Size: 919 3244
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 404 880 911 989 1594 1842 2544 4040 404
Size: 476 31 78 605 248 702 1496 527
Fragments arranged by size:
1496 702 605 527 476 248 78 31
BsaWI w'CCGG_w
Cuts at: 496 683 1352 1873 2551 2825 2972 3383 496
Size: 187 669 521 678 274 147 411 1276
Fragments arranged by size:
1276 678 669 521 411 274 187 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 547 577 547
Size: 30 4133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 454 145
Size: 309 3854
BseYI C'CCAG_C
Cuts at: 115 1588 2346 2923 3419 115
Size: 1473 758 577 496 859
Fragments arranged by size:
1473 859 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 820 1030 374
Size: 446 210 3507
Fragments arranged by size:
3507 446 210
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2959 104
Size: 1601 46 1208 1308
Fragments arranged by size:
1601 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 238 893 1025 1177 1955 2145 2937 238
Size: 655 132 152 778 190 792 1464
Fragments arranged by size:
1464 792 778 655 190 152 132
BsiWI C'GTAC_G
Cuts at: 348 348
Size: 4163
BsmBI CGTCTCn'nnnn_
Cuts at: 646 646
Size: 4163
Bsp1286I G_dGCh'C
Cuts at: 238 448 602 893 1025 1177 1788 1881 1955
Size: 210 154 291 132 152 611 93 74
Cuts at: 1955 2145 2207 2937 238
Size: 190 62 730 1464
Fragments arranged by size:
1464 730 611 291 210 190 154 152
132 93 74 62
BspEI T'CCGG_A
Cuts at: 683 3383 683
Size: 2700 1463
BspHI T'CATG_A
Cuts at: 3339 3339
Size: 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 760 1213 1427 1729 2110 760
Size: 453 214 302 381 2813
Fragments arranged by size:
2813 453 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2075 2075
Size: 4163
BsrFI r'CCGG_y
Cuts at: 217 991 2161 2342 2551 217
Size: 774 1170 181 209 1829
Fragments arranged by size:
1829 1170 774 209 181
BssHII G'CGCG_C
Cuts at: 519 519
Size: 4163
BssSI C'ACGA_G
Cuts at: 826 2434 2792 826
Size: 1608 358 2197
Fragments arranged by size:
2197 1608 358
BstAPI GCAn_nnn'nTGC
Cuts at: 615 1540 615
Size: 925 3238
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstEII G'GTnAC_C
Cuts at: 582 582
Size: 4163
BstXI CCAn_nnnn'nTGG
Cuts at: 150 150
Size: 4163
BstYI r'GATC_y
Cuts at: 1 672 728 1049 1188 1655 1678 2013 2259
Size: 671 56 321 139 467 23 335 246
Cuts at: 2259 3271 3357 3369 3715 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 671 467 449 346 335 321 246
139 86 56 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 750 2113 2294 2457 750
Size: 1363 181 163 2456
Fragments arranged by size:
2456 1363 181 163
BtsI GCAGTG_nn'
Cuts at: 732 1406 732
Size: 674 3489
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1128 1163 1128
Size: 35 4128
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 341 1748 1922 2313 2340 3685 341
Size: 1407 174 391 27 1345 819
Fragments arranged by size:
1407 1345 819 391 174 27
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 226 281 898 1159 2186 2396 226
Size: 55 617 261 1027 210 1993
Fragments arranged by size:
1993 1027 617 261 210 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2371 2681 2827 3973 2371
Size: 310 146 1146 2561
Fragments arranged by size:
2561 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1023 1175 1023
Size: 152 4011
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 592 1988 2420 3167 3435 126
Size: 466 1396 432 747 268 854
Fragments arranged by size:
1396 854 747 466 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1302 1302
Size: 4163
EcoRI G'AATT_C
Cuts at: 1167 1167
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 930 2182 930
Size: 1252 2911
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 579 711 775 782 1597 1845 2867 3846 579
Size: 132 64 7 815 248 1022 979 896
Fragments arranged by size:
1022 979 896 815 248 132 64 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 262 294 2005 2035 2037 2067 262
Size: 32 1711 30 2 30 2358
Fragments arranged by size:
2358 1711 32 30 30 2
HincII GTy'rAC
Cuts at: 1202 1202
Size: 4163
KasI G'GCGC_C
Cuts at: 1593 1841 1593
Size: 248 3915
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 446 590 2834 3018 446
Size: 144 2244 184 1591
Fragments arranged by size:
2244 1591 184 144
MscI TGG'CCA
Cuts at: 343 1924 343
Size: 1581 2582
MslI CAynn'nnrTG
Cuts at: 1006 2152 2279 1006
Size: 1146 127 2890
Fragments arranged by size:
2890 1146 127
MspA1I CmG'CkG
Cuts at: 480 1274 1588 1948 2961 3206 480
Size: 794 314 360 1013 245 1437
Fragments arranged by size:
1437 1013 794 360 314 245
NaeI GCC'GGC
Cuts at: 993 2344 993
Size: 1351 2812
NarI GG'CG_CC
Cuts at: 1594 1842 1594
Size: 248 3915
NcoI C'CATG_G
Cuts at: 107 107
Size: 4163
NgoMIV G'CCGG_C
Cuts at: 991 2342 991
Size: 1351 2812
NruI TCG'CGA
Cuts at: 627 627
Size: 4163
NspI r_CATG'y
Cuts at: 825 1032 1216 1716 2623 825
Size: 207 184 500 907 2365
Fragments arranged by size:
2365 907 500 207 184
PciI A'CATG_T
Cuts at: 2619 2619
Size: 4163
PfoI T'CCnGG_A
Cuts at: 226 956 226
Size: 730 3433
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 643 675 3302 3334 643
Size: 32 2627 32 1472
Fragments arranged by size:
2627 1472 32 32
PspXI vC'TCGA_Gb
Cuts at: 295 1018 295
Size: 723 3440
PstI C_TGCA'G
Cuts at: 1210 1895 1210
Size: 685 3478
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 1588 1948 1588
Size: 360 3803
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1025 1177 1025
Size: 152 4011
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 226 898 2186 2396 226
Size: 672 1288 210 1993
Fragments arranged by size:
1993 1288 672 210
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SfcI C'TryA_G
Cuts at: 33 1206 1891 2884 3075 33
Size: 1173 685 993 191 1121
Fragments arranged by size:
1173 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1595 1843 1595
Size: 248 3915
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 590 1185 590
Size: 595 3568
SmlI C'TyrA_G
Cuts at: 295 1018 1318 2233 2725 2987 295
Size: 723 300 915 492 262 1471
Fragments arranged by size:
1471 915 723 492 300 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 825 1216 1716 825
Size: 391 500 3272
Fragments arranged by size:
3272 500 391
StuI AGG'CCT
Cuts at: 508 508
Size: 4163
StyI C'CwwG_G
Cuts at: 107 455 1297 1651 107
Size: 348 842 354 2619
Fragments arranged by size:
2619 842 354 348
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 202 1746 1879 2338 2547 202
Size: 1544 133 459 209 1818
Fragments arranged by size:
1818 1544 459 209 133
TatI w'GTAC_w
Cuts at: 3678 3678
Size: 4163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 356 388 3680 3712 356
Size: 32 3292 32 807
Fragments arranged by size:
3292 807 32 32
Tth111I GACn'n_nGTC
Cuts at: 1108 1960 1108
Size: 852 3311
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1658 1658
Size: 4163
XhoI C'TCGA_G
Cuts at: 295 1018 295
Size: 723 3440
XmaI C'CCGG_G
Cuts at: 588 1183 588
Size: 595 3568
ZraI GAC'GTC
Cuts at: 881 912 881
Size: 31 4132
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PfoI PmeI
PpiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AloI ApaI AscI AvrII
BcgI BfrBI BmtI BsmI BsrGI BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XmnI