Restriction map for
pF1KE3103
(Circular) (Six-base) MAPSORT of: pF1KE3103.seq Check: 6990 from: 1 to: 3920
pF1KE3103 3920 bp
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 510 510
Size: 3920
AccI GT'mk_AC
Cuts at: 958 958
Size: 3920
Acc65I G'GTAC_C
Cuts at: 936 936
Size: 3920
AclI AA'CG_TT
Cuts at: 3760 3760
Size: 3920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 273 1745 2177 2924 3192 108
Size: 165 1472 432 747 268 836
Fragments arranged by size:
1472 836 747 432 268 165
AflIII A'CryG_T
Cuts at: 1272 2376 3353 1272
Size: 1104 977 1839
Fragments arranged by size:
1839 1104 977
AgeI A'CCGG_T
Cuts at: 2308 2308
Size: 3920
AlwNI CAG_nnn'CTG
Cuts at: 1225 2792 1225
Size: 1567 2353
ApaI G_GGCC'C
Cuts at: 231 398 439 445 667 231
Size: 167 41 6 222 3484
Fragments arranged by size:
3484 222 167 41 6
ApaLI G'TGCA_C
Cuts at: 2690 2690
Size: 3920
ApoI r'AATT_y
Cuts at: 924 3273 3529 924
Size: 2349 256 1315
Fragments arranged by size:
2349 1315 256
AscI GG'CGCG_CC
Cuts at: 149 149
Size: 3920
AseI AT'TA_AT
Cuts at: 20 20
Size: 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
AvaI C'yCGr_G
Cuts at: 438 638 682 940 438
Size: 200 44 258 3418
Fragments arranged by size:
3418 258 200 44
AvrII C'CTAG_G
Cuts at: 231 231
Size: 3920
BamHI G'GATC_C
Cuts at: 945 945
Size: 3920
BanI G'GyrC_C
Cuts at: 244 936 1350 1598 1633 244
Size: 692 414 248 35 2531
Fragments arranged by size:
2531 692 414 248 35
BanII G_rGCy'C
Cuts at: 231 350 398 439 445 476 667 934 1964
Size: 119 48 41 6 31 191 267 1030
Cuts at: 1964 231
Size: 2187
Fragments arranged by size:
2187 1030 267 191 119 48 41 31
6
BbeI G_GCGC'C
Cuts at: 248 1354 1602 248
Size: 1106 248 2566
Fragments arranged by size:
2566 1106 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 696 730 696
Size: 34 3886
BciVI GTATCCnnnnn_n'
Cuts at: 1818 2579 3920 1818
Size: 761 1341 1818
Fragments arranged by size:
1818 1341 761
BclI T'GATC_A
Cuts at: 1440 1440
Size: 3920
BglI GCCn_nnn'nGGC
Cuts at: 134 197 434 134
Size: 63 237 3620
Fragments arranged by size:
3620 237 63
BglII A'GATC_T
Cuts at: 1435 3472 1435
Size: 2037 1883
BlpI GC'TnA_GC
Cuts at: 1032 1032
Size: 3920
Bme1580I G_kGCm'C
Cuts at: 231 323 398 439 445 667 1545 1638 2694
Size: 92 75 41 6 222 878 93 1056
Cuts at: 2694 231
Size: 1457
Fragments arranged by size:
1457 1056 878 222 93 92 75 41
6
BmgBI CAC'GTC
Cuts at: 584 584
Size: 3920
BmrI ACTGGGnnnn_n'
Cuts at: 489 1132 1213 1548 3692 489
Size: 643 81 335 2144 717
Fragments arranged by size:
2144 717 643 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 3920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 421 349
Size: 72 3848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1096 1975 2467 2765 448
Size: 648 879 492 298 1603
Fragments arranged by size:
1603 879 648 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3624 3624
Size: 3920
BsaAI yAC'GTr
Cuts at: 1273 1273
Size: 3920
BsaBI GATnn'nnATC
Cuts at: 6 1434 3471 6
Size: 1428 2037 455
Fragments arranged by size:
2037 1428 455
BsaHI Gr'CG_yC
Cuts at: 245 1351 1599 2301 3797 245
Size: 1106 248 702 1496 368
Fragments arranged by size:
1496 1106 702 368 248
BsaWI w'CCGG_w
Cuts at: 1109 1630 2308 2582 2729 3140 1109
Size: 521 678 274 147 411 1889
Fragments arranged by size:
1889 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 289 319 289
Size: 30 3890
BseYI C'CCAG_C
Cuts at: 871 1345 2103 2680 3176 871
Size: 474 758 577 496 1615
Fragments arranged by size:
1615 758 577 496 474
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 790
Size: 3920
BsiEI CG_ry'CG
Cuts at: 104 560 893 1462 1508 2716 104
Size: 456 333 569 46 1208 1308
Fragments arranged by size:
1308 1208 569 456 333 46
BsiHKAI G_wGCw'C
Cuts at: 350 934 1712 1902 2694 350
Size: 584 778 190 792 1576
Fragments arranged by size:
1576 792 778 584 190
Bsp1286I G_dGCh'C
Cuts at: 231 323 350 398 439 445 476 667 934
Size: 92 27 48 41 6 31 191 267
Cuts at: 934 1545 1638 1712 1902 1964 2694 231
Size: 611 93 74 190 62 730 1457
Fragments arranged by size:
1457 730 611 267 191 190 93 92
74 62 48 41 31 27 6
BspEI T'CCGG_A
Cuts at: 3140 3140
Size: 3920
BspHI T'CATG_A
Cuts at: 3096 3096
Size: 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 510 970 1184 1486 1867 510
Size: 460 214 302 381 2563
Fragments arranged by size:
2563 460 381 302 214
BsrBI CCG'CTC
Cuts at: 50 451 2212 2266 3750 3915 50
Size: 401 1761 54 1484 165 55
Fragments arranged by size:
1761 1484 401 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1832 1832
Size: 3920
BsrFI r'CCGG_y
Cuts at: 652 689 841 888 1918 2099 2308 652
Size: 37 152 47 1030 181 209 2264
Fragments arranged by size:
2264 1030 209 181 152 47 37
BssHII G'CGCG_C
Cuts at: 142 149 424 142
Size: 7 275 3638
Fragments arranged by size:
3638 275 7
BssSI C'ACGA_G
Cuts at: 2191 2549 2191
Size: 358 3562
BstAPI GCAn_nnn'nTGC
Cuts at: 1297 1297
Size: 3920
BstBI TT'CG_AA
Cuts at: 2281 2281
Size: 3920
BstYI r'GATC_y
Cuts at: 1 945 1412 1435 1770 2016 3028 3114 3126
Size: 944 467 23 335 246 1012 86 12
Cuts at: 3126 3472 1
Size: 346 449
Fragments arranged by size:
1012 944 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 210 308 542 672 210
Size: 98 234 130 3458
Fragments arranged by size:
3458 234 130 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 699 1870 2051 2214 699
Size: 1171 181 163 2405
Fragments arranged by size:
2405 1171 181 163
BtsI GCAGTG_nn'
Cuts at: 1163 1163
Size: 3920
DraI TTT'AAA
Cuts at: 919 919
Size: 3920
DrdI GACnn_nn'nnGTC
Cuts at: 1626 2484 1626
Size: 858 3062
EaeI y'GGCC_r
Cuts at: 311 524 557 839 890 1505 1679 2070 2097
Size: 213 33 282 51 615 174 391 27
Cuts at: 2097 3442 311
Size: 1345 789
Fragments arranged by size:
1345 789 615 391 282 213 174 51
33 27
EagI C'GGCC_G
Cuts at: 557 890 1505 557
Size: 333 615 2972
Fragments arranged by size:
2972 615 333
EarI CTCTTCn'nnn_
Cuts at: 127 355 367 640 1943 2153 127
Size: 228 12 273 1303 210 1894
Fragments arranged by size:
1894 1303 273 228 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2128 2438 2584 3730 2128
Size: 310 146 1146 2318
Fragments arranged by size:
2318 1146 310 146
EcoICRI GAG'CTC
Cuts at: 348 932 348
Size: 584 3336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 273 349 421 1745 2177 2924 3192 108
Size: 165 76 72 1324 432 747 268 836
Fragments arranged by size:
1324 836 747 432 268 165 76 72
EcoO109I rG'GnC_Cy
Cuts at: 227 228 394 395 442 663 834 1059 227
Size: 1 166 1 47 221 171 225 3088
Fragments arranged by size:
3088 225 221 171 166 47 1 1
EcoRI G'AATT_C
Cuts at: 924 924
Size: 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1939 1939
Size: 3920
FseI GG_CCGG'CC
Cuts at: 845 845
Size: 3920
FspI TGC'GCA
Cuts at: 534 724 1701 534
Size: 190 977 2753
Fragments arranged by size:
2753 977 190
FspAI rTGC'GCAy
Cuts at: 534 534
Size: 3920
HaeII r_GCGC'y
Cuts at: 116 248 281 296 653 1354 1602 2624 3603
Size: 132 33 15 357 701 248 1022 979
Cuts at: 3603 116
Size: 433
Fragments arranged by size:
1022 979 701 433 357 248 132 33
15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1762 1792 1794 1824 1762
Size: 30 2 30 3858
Fragments arranged by size:
3858 30 30 2
HincII GTy'rAC
Cuts at: 959 959
Size: 3920
KasI G'GCGC_C
Cuts at: 244 1350 1598 244
Size: 1106 248 2566
Fragments arranged by size:
2566 1106 248
KpnI G_GTAC'C
Cuts at: 940 940
Size: 3920
MluI A'CGCG_T
Cuts at: 3353 3353
Size: 3920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2591 2775 2591
Size: 184 3736
MscI TGG'CCA
Cuts at: 1681 1681
Size: 3920
MslI CAynn'nnrTG
Cuts at: 718 1909 2036 718
Size: 1191 127 2602
Fragments arranged by size:
2602 1191 127
MspA1I CmG'CkG
Cuts at: 212 674 696 867 1031 1345 1705 2718 2963
Size: 462 22 171 164 314 360 1013 245
Cuts at: 2963 212
Size: 1169
Fragments arranged by size:
1169 1013 462 360 314 245 171 164
22
NaeI GCC'GGC
Cuts at: 654 843 890 2101 654
Size: 189 47 1211 2473
Fragments arranged by size:
2473 1211 189 47
NarI GG'CG_CC
Cuts at: 245 1351 1599 245
Size: 1106 248 2566
Fragments arranged by size:
2566 1106 248
NcoI C'CATG_G
Cuts at: 308 308
Size: 3920
NgoMIV G'CCGG_C
Cuts at: 652 841 888 2099 652
Size: 189 47 1211 2473
Fragments arranged by size:
2473 1211 189 47
NotI GC'GGCC_GC
Cuts at: 557 557
Size: 3920
NspI r_CATG'y
Cuts at: 973 1473 2380 973
Size: 500 907 2513
Fragments arranged by size:
2513 907 500
PciI A'CATG_T
Cuts at: 2376 2376
Size: 3920
PflMI CCAn_nnn'nTGG
Cuts at: 816 816
Size: 3920
PmeI GTTT'AAAC
Cuts at: 919 919
Size: 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3888
PpuMI rG'GwC_Cy
Cuts at: 834 834
Size: 3920
PspOMI G'GGCC_C
Cuts at: 227 394 435 441 663 227
Size: 167 41 6 222 3484
Fragments arranged by size:
3484 222 167 41 6
PstI C_TGCA'G
Cuts at: 967 1652 967
Size: 685 3235
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3920
PvuII CAG'CTG
Cuts at: 867 1345 1705 867
Size: 478 360 3082
Fragments arranged by size:
3082 478 360
RsrII CG'GwC_CG
Cuts at: 669 2115 669
Size: 1446 2474
SacI G_AGCT'C
Cuts at: 350 934 350
Size: 584 3336
SacII CC_GC'GG
Cuts at: 213 675 213
Size: 462 3458
SalI G'TCGA_C
Cuts at: 957 957
Size: 3920
SapI GCTCTTCn'nnn_
Cuts at: 127 355 640 1943 2153 127
Size: 228 285 1303 210 1894
Fragments arranged by size:
1894 1303 285 228 210
SbfI CC_TGCA'GG
Cuts at: 967 967
Size: 3920
ScaI AGT'ACT
Cuts at: 3437 3437
Size: 3920
SfcI C'TryA_G
Cuts at: 33 963 1648 2641 2832 33
Size: 930 685 993 191 1121
Fragments arranged by size:
1121 993 930 685 191
SfoI GGC'GCC
Cuts at: 246 1352 1600 246
Size: 1106 248 2566
Fragments arranged by size:
2566 1106 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
SmaI CCC'GGG
Cuts at: 440 640 942 440
Size: 200 302 3418
Fragments arranged by size:
3418 302 200
SmlI C'TyrA_G
Cuts at: 463 1075 1990 2482 2744 463
Size: 612 915 492 262 1639
Fragments arranged by size:
1639 915 612 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3920
SphI G_CATG'C
Cuts at: 973 1473 973
Size: 500 3420
SrfI GCCC'GGGC
Cuts at: 440 440
Size: 3920
StyI C'CwwG_G
Cuts at: 231 308 431 1054 1408 231
Size: 77 123 623 354 2743
Fragments arranged by size:
2743 623 354 123 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1503 1636 2095 2304 1503
Size: 133 459 209 3119
Fragments arranged by size:
3119 459 209 133
TatI w'GTAC_w
Cuts at: 3435 3435
Size: 3920
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 3888
Tth111I GACn'n_nGTC
Cuts at: 1717 1717
Size: 3920
XbaI T'CTAG_A
Cuts at: 951 951
Size: 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1415 1415
Size: 3920
XmaI C'CCGG_G
Cuts at: 438 638 940 438
Size: 200 302 3418
Fragments arranged by size:
3418 302 200
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FseI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI BaeI
BbsI BbvCI BfrBI BmtI Bpu10I BseRI BsiWI BsmI
BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV HindIII HpaI MfeI NdeI NheI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI XmnI ZraI