Restriction map for
pF1KE3023
(Circular) (Six-base) MAPSORT of: pF1KE3023.seq Check: 3688 from: 1 to: 3527
pF1KE3023 3527 bp
With 182 enzymes: SgfI *
June 20, 2019 10:05 ..
AccI GT'mk_AC
Cuts at: 187 565 187
Size: 378 3149
Acc65I G'GTAC_C
Cuts at: 543 543
Size: 3527
AclI AA'CG_TT
Cuts at: 3367 3367
Size: 3527
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1352 1784 2531 2799 1352
Size: 432 747 268 2080
Fragments arranged by size:
2080 747 432 268
AflIII A'CryG_T
Cuts at: 879 1983 2960 879
Size: 1104 977 1446
Fragments arranged by size:
1446 1104 977
AgeI A'CCGG_T
Cuts at: 1915 1915
Size: 3527
AlwNI CAG_nnn'CTG
Cuts at: 832 2399 832
Size: 1567 1960
ApaLI G'TGCA_C
Cuts at: 2297 2297
Size: 3527
ApoI r'AATT_y
Cuts at: 531 2880 3136 531
Size: 2349 256 922
Fragments arranged by size:
2349 922 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3527
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3527
AvaI C'yCGr_G
Cuts at: 547 547
Size: 3527
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 465 498 465
Size: 33 3494
BamHI G'GATC_C
Cuts at: 552 552
Size: 3527
BanI G'GyrC_C
Cuts at: 543 957 1205 1240 543
Size: 414 248 35 2830
Fragments arranged by size:
2830 414 248 35
BanII G_rGCy'C
Cuts at: 541 1571 541
Size: 1030 2497
BbeI G_GCGC'C
Cuts at: 961 1209 961
Size: 248 3279
BciVI GTATCCnnnnn_n'
Cuts at: 1425 2186 3527 1425
Size: 761 1341 1425
Fragments arranged by size:
1425 1341 761
BclI T'GATC_A
Cuts at: 1047 1047
Size: 3527
BglII A'GATC_T
Cuts at: 1042 3079 1042
Size: 2037 1490
BlpI GC'TnA_GC
Cuts at: 639 639
Size: 3527
Bme1580I G_kGCm'C
Cuts at: 1152 1245 2301 1152
Size: 93 1056 2378
Fragments arranged by size:
2378 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 739 820 1155 3299 739
Size: 81 335 2144 967
Fragments arranged by size:
2144 967 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1894 1894
Size: 3527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1582 2074 2372 703
Size: 879 492 298 1858
Fragments arranged by size:
1858 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3231 3231
Size: 3527
BsaAI yAC'GTr
Cuts at: 880 880
Size: 3527
BsaBI GATnn'nnATC
Cuts at: 6 1041 3078 6
Size: 1035 2037 455
Fragments arranged by size:
2037 1035 455
BsaHI Gr'CG_yC
Cuts at: 958 1206 1908 3404 958
Size: 248 702 1496 1081
Fragments arranged by size:
1496 1081 702 248
BsaWI w'CCGG_w
Cuts at: 716 1237 1915 2189 2336 2747 716
Size: 521 678 274 147 411 1496
Fragments arranged by size:
1496 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 198 228 198
Size: 30 3497
BseYI C'CCAG_C
Cuts at: 952 1710 2287 2783 952
Size: 758 577 496 1696
Fragments arranged by size:
1696 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1069 1115 2323 104
Size: 965 46 1208 1308
Fragments arranged by size:
1308 1208 965 46
BsiHKAI G_wGCw'C
Cuts at: 541 1319 1509 2301 541
Size: 778 190 792 1767
Fragments arranged by size:
1767 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 541 1152 1245 1319 1509 1571 2301 541
Size: 611 93 74 190 62 730 1767
Fragments arranged by size:
1767 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2747 2747
Size: 3527
BspHI T'CATG_A
Cuts at: 2703 2703
Size: 3527
BspMI ACCTGCnnnn'nnnn_
Cuts at: 577 791 1093 1474 577
Size: 214 302 381 2630
Fragments arranged by size:
2630 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1819 1873 3357 3522 50
Size: 1769 54 1484 165 55
Fragments arranged by size:
1769 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1439 1439
Size: 3527
BsrFI r'CCGG_y
Cuts at: 1525 1706 1915 1525
Size: 181 209 3137
Fragments arranged by size:
3137 209 181
BssSI C'ACGA_G
Cuts at: 1798 2156 1798
Size: 358 3169
BstAPI GCAn_nnn'nTGC
Cuts at: 904 904
Size: 3527
BstBI TT'CG_AA
Cuts at: 1888 1888
Size: 3527
BstYI r'GATC_y
Cuts at: 1 552 1019 1042 1377 1623 2635 2721 2733
Size: 551 467 23 335 246 1012 86 12
Cuts at: 2733 3079 1
Size: 346 449
Fragments arranged by size:
1012 551 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 188 188
Size: 3527
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 175 1477 1658 1821 175
Size: 1302 181 163 1881
Fragments arranged by size:
1881 1302 181 163
BtsI GCAGTG_nn'
Cuts at: 770 770
Size: 3527
DraI TTT'AAA
Cuts at: 526 526
Size: 3527
DrdI GACnn_nn'nnGTC
Cuts at: 1233 2091 1233
Size: 858 2669
EaeI y'GGCC_r
Cuts at: 1112 1286 1677 1704 3049 1112
Size: 174 391 27 1345 1590
Fragments arranged by size:
1590 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1112 1112
Size: 3527
EarI CTCTTCn'nnn_
Cuts at: 1550 1760 1550
Size: 210 3317
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1735 2045 2191 3337 1735
Size: 310 146 1146 1925
Fragments arranged by size:
1925 1146 310 146
EcoICRI GAG'CTC
Cuts at: 539 539
Size: 3527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1352 1784 2531 2799 1352
Size: 432 747 268 2080
Fragments arranged by size:
2080 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 666 666
Size: 3527
EcoRI G'AATT_C
Cuts at: 531 531
Size: 3527
EcoRV GAT'ATC
Cuts at: 273 273
Size: 3527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1546 1546
Size: 3527
FspI TGC'GCA
Cuts at: 1308 1308
Size: 3527
HaeII r_GCGC'y
Cuts at: 961 1209 2231 3210 961
Size: 248 1022 979 1278
Fragments arranged by size:
1278 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 323 355 356 388 503 535 1369 1399 1401
Size: 32 1 32 115 32 834 30 2
Cuts at: 1401 1431 323
Size: 30 2419
Fragments arranged by size:
2419 834 115 32 32 32 30 30
2 1
HincII GTy'rAC
Cuts at: 566 566
Size: 3527
KasI G'GCGC_C
Cuts at: 957 1205 957
Size: 248 3279
KpnI G_GTAC'C
Cuts at: 547 547
Size: 3527
MluI A'CGCG_T
Cuts at: 2960 2960
Size: 3527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 202 2198 2382 202
Size: 1996 184 1347
Fragments arranged by size:
1996 1347 184
MscI TGG'CCA
Cuts at: 1288 1288
Size: 3527
MslI CAynn'nnrTG
Cuts at: 1516 1643 1516
Size: 127 3400
MspA1I CmG'CkG
Cuts at: 638 952 1312 2325 2570 638
Size: 314 360 1013 245 1595
Fragments arranged by size:
1595 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1708 1708
Size: 3527
NarI GG'CG_CC
Cuts at: 958 1206 958
Size: 248 3279
NgoMIV G'CCGG_C
Cuts at: 1706 1706
Size: 3527
NspI r_CATG'y
Cuts at: 152 580 1080 1987 152
Size: 428 500 907 1692
Fragments arranged by size:
1692 907 500 428
PciI A'CATG_T
Cuts at: 1983 1983
Size: 3527
PmeI GTTT'AAAC
Cuts at: 526 526
Size: 3527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2666 2698 2666
Size: 32 3495
PstI C_TGCA'G
Cuts at: 574 1259 574
Size: 685 2842
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3527
PvuII CAG'CTG
Cuts at: 952 1312 952
Size: 360 3167
RsrII CG'GwC_CG
Cuts at: 1722 1722
Size: 3527
SacI G_AGCT'C
Cuts at: 541 541
Size: 3527
SalI G'TCGA_C
Cuts at: 564 564
Size: 3527
SapI GCTCTTCn'nnn_
Cuts at: 1550 1760 1550
Size: 210 3317
SbfI CC_TGCA'GG
Cuts at: 574 574
Size: 3527
ScaI AGT'ACT
Cuts at: 3044 3044
Size: 3527
SfcI C'TryA_G
Cuts at: 33 342 350 441 570 1255 2248 2439 33
Size: 309 8 91 129 685 993 191 1121
Fragments arranged by size:
1121 993 685 309 191 129 91 8
SfoI GGC'GCC
Cuts at: 959 1207 959
Size: 248 3279
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3527
SmaI CCC'GGG
Cuts at: 549 549
Size: 3527
SmlI C'TyrA_G
Cuts at: 682 1597 2089 2351 682
Size: 915 492 262 1858
Fragments arranged by size:
1858 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3527
SphI G_CATG'C
Cuts at: 580 1080 580
Size: 500 3027
StyI C'CwwG_G
Cuts at: 661 1015 661
Size: 354 3173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1110 1243 1702 1911 1110
Size: 133 459 209 2726
Fragments arranged by size:
2726 459 209 133
TatI w'GTAC_w
Cuts at: 3042 3042
Size: 3527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 198 230 3044 3076 198
Size: 32 2814 32 649
Fragments arranged by size:
2814 649 32 32
Tth111I GACn'n_nGTC
Cuts at: 1324 1324
Size: 3527
XbaI T'CTAG_A
Cuts at: 558 558
Size: 3527
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1022 1022
Size: 3527
XmaI C'CCGG_G
Cuts at: 547 547
Size: 3527
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BstZ17I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI Bsu36I BtgI ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI