Restriction map for
pF1KE2788
(Circular) (Six-base) MAPSORT of: pF1KE2788.seq Check: 6015 from: 1 to: 4016
pF1KE2788 4016 bp
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 174 174
Size: 4016
AccI GT'mk_AC
Cuts at: 854 1054 854
Size: 200 3816
Acc65I G'GTAC_C
Cuts at: 1032 1032
Size: 4016
AclI AA'CG_TT
Cuts at: 3856 3856
Size: 4016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 595 1841 2273 3020 3288 290
Size: 305 1246 432 747 268 1018
Fragments arranged by size:
1246 1018 747 432 305 268
AflIII A'CryG_T
Cuts at: 1368 2472 3449 1368
Size: 1104 977 1935
Fragments arranged by size:
1935 1104 977
AgeI A'CCGG_T
Cuts at: 2404 2404
Size: 4016
AlwNI CAG_nnn'CTG
Cuts at: 825 1321 2888 825
Size: 496 1567 1953
Fragments arranged by size:
1953 1567 496
ApaLI G'TGCA_C
Cuts at: 2786 2786
Size: 4016
ApoI r'AATT_y
Cuts at: 213 1020 3369 3625 213
Size: 807 2349 256 604
Fragments arranged by size:
2349 807 604 256
AseI AT'TA_AT
Cuts at: 20 518 20
Size: 498 3518
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
AvaI C'yCGr_G
Cuts at: 1036 1036
Size: 4016
BamHI G'GATC_C
Cuts at: 1041 1041
Size: 4016
BanI G'GyrC_C
Cuts at: 1032 1446 1694 1729 1032
Size: 414 248 35 3319
Fragments arranged by size:
3319 414 248 35
BanII G_rGCy'C
Cuts at: 1030 2060 1030
Size: 1030 2986
BbeI G_GCGC'C
Cuts at: 1450 1698 1450
Size: 248 3768
BbsI GAAGACnn'nnnn_
Cuts at: 859 859
Size: 4016
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3982
BciVI GTATCCnnnnn_n'
Cuts at: 1914 2675 4016 1914
Size: 761 1341 1914
Fragments arranged by size:
1914 1341 761
BclI T'GATC_A
Cuts at: 1536 1536
Size: 4016
BglII A'GATC_T
Cuts at: 1531 3568 1531
Size: 2037 1979
BlpI GC'TnA_GC
Cuts at: 1128 1128
Size: 4016
Bme1580I G_kGCm'C
Cuts at: 1641 1734 2790 1641
Size: 93 1056 2867
Fragments arranged by size:
2867 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 620 1228 1309 1644 3788 620
Size: 608 81 335 2144 848
Fragments arranged by size:
2144 848 608 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 769 913 180
Size: 589 144 3283
Fragments arranged by size:
3283 589 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1192 2071 2563 2861 157
Size: 1035 879 492 298 1312
Fragments arranged by size:
1312 1035 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 427 889 3720 427
Size: 462 2831 723
Fragments arranged by size:
2831 723 462
BsaAI yAC'GTr
Cuts at: 273 1369 273
Size: 1096 2920
BsaBI GATnn'nnATC
Cuts at: 6 1530 3567 6
Size: 1524 2037 455
Fragments arranged by size:
2037 1524 455
BsaHI Gr'CG_yC
Cuts at: 829 1447 1695 2397 3893 829
Size: 618 248 702 1496 952
Fragments arranged by size:
1496 952 702 618 248
BsaWI w'CCGG_w
Cuts at: 1205 1726 2404 2678 2825 3236 1205
Size: 521 678 274 147 411 1985
Fragments arranged by size:
1985 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 220
Size: 4016
BseYI C'CCAG_C
Cuts at: 190 1441 2199 2776 3272 190
Size: 1251 758 577 496 934
Fragments arranged by size:
1251 934 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1558 1604 2812 104
Size: 1454 46 1208 1308
Fragments arranged by size:
1454 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1030 1808 1998 2790 1030
Size: 778 190 792 2256
Fragments arranged by size:
2256 792 778 190
BsmI GAATG_Cn'
Cuts at: 312 312
Size: 4016
Bsp1286I G_dGCh'C
Cuts at: 1030 1641 1734 1808 1998 2060 2790 1030
Size: 611 93 74 190 62 730 2256
Fragments arranged by size:
2256 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3236 3236
Size: 4016
BspHI T'CATG_A
Cuts at: 801 842 3192 801
Size: 41 2350 1625
Fragments arranged by size:
2350 1625 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 174 1066 1280 1582 1963 174
Size: 892 214 302 381 2227
Fragments arranged by size:
2227 892 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2308 2362 3846 4011 50
Size: 2258 54 1484 165 55
Fragments arranged by size:
2258 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1005 1928 1005
Size: 923 3093
BsrFI r'CCGG_y
Cuts at: 2014 2195 2404 2014
Size: 181 209 3626
Fragments arranged by size:
3626 209 181
BssSI C'ACGA_G
Cuts at: 2287 2645 2287
Size: 358 3658
BstAPI GCAn_nnn'nTGC
Cuts at: 1393 1393
Size: 4016
BstBI TT'CG_AA
Cuts at: 2377 2377
Size: 4016
BstYI r'GATC_y
Cuts at: 1 1041 1508 1531 1866 2112 3124 3210 3222
Size: 1040 467 23 335 246 1012 86 12
Cuts at: 3222 3568 1
Size: 346 449
Fragments arranged by size:
1040 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 599 599
Size: 4016
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1966 2147 2310 1966
Size: 181 163 3672
Fragments arranged by size:
3672 181 163
BtsI GCAGTG_nn'
Cuts at: 737 1259 737
Size: 522 3494
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 145 180 145
Size: 35 3981
DraI TTT'AAA
Cuts at: 1015 1015
Size: 4016
DrdI GACnn_nn'nnGTC
Cuts at: 1722 2580 1722
Size: 858 3158
EaeI y'GGCC_r
Cuts at: 512 1601 1775 2166 2193 3538 512
Size: 1089 174 391 27 1345 990
Fragments arranged by size:
1345 1089 990 391 174 27
EagI C'GGCC_G
Cuts at: 1601 1601
Size: 4016
EarI CTCTTCn'nnn_
Cuts at: 197 309 2039 2249 197
Size: 112 1730 210 1964
Fragments arranged by size:
1964 1730 210 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2224 2534 2680 3826 2224
Size: 310 146 1146 2414
Fragments arranged by size:
2414 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1028 1028
Size: 4016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 290 595 769 913 1841 2273 3020 3288
Size: 110 305 174 144 928 432 747 268
Cuts at: 3288 180
Size: 908
Fragments arranged by size:
928 908 747 432 305 268 174 144
110
EcoO109I rG'GnC_Cy
Cuts at: 1155 1155
Size: 4016
EcoRI G'AATT_C
Cuts at: 1020 1020
Size: 4016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 4016
FspI TGC'GCA
Cuts at: 1797 1797
Size: 4016
HaeII r_GCGC'y
Cuts at: 1450 1698 2720 3699 1450
Size: 248 1022 979 1767
Fragments arranged by size:
1767 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 526 558 902 934 1858 1888 1890 1920 526
Size: 32 344 32 924 30 2 30 2622
Fragments arranged by size:
2622 924 344 32 32 30 30 2
HincII GTy'rAC
Cuts at: 947 1055 947
Size: 108 3908
KasI G'GCGC_C
Cuts at: 1446 1694 1446
Size: 248 3768
KpnI G_GTAC'C
Cuts at: 1036 1036
Size: 4016
MfeI C'AATT_G
Cuts at: 937 937
Size: 4016
MluI A'CGCG_T
Cuts at: 3449 3449
Size: 4016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2687 2871 2687
Size: 184 3832
MscI TGG'CCA
Cuts at: 514 1777 514
Size: 1263 2753
MslI CAynn'nnrTG
Cuts at: 2005 2132 2005
Size: 127 3889
MspA1I CmG'CkG
Cuts at: 654 1127 1441 1801 2814 3059 654
Size: 473 314 360 1013 245 1611
Fragments arranged by size:
1611 1013 473 360 314 245
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 4016
NarI GG'CG_CC
Cuts at: 1447 1695 1447
Size: 248 3768
NcoI C'CATG_G
Cuts at: 599 599
Size: 4016
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 4016
NspI r_CATG'y
Cuts at: 1069 1569 2476 1069
Size: 500 907 2609
Fragments arranged by size:
2609 907 500
PciI A'CATG_T
Cuts at: 2472 2472
Size: 4016
PflMI CCAn_nnn'nTGG
Cuts at: 510 825 510
Size: 315 3701
PmeI GTTT'AAAC
Cuts at: 1015 1015
Size: 4016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3984
PsiI TTA'TAA
Cuts at: 176 479 176
Size: 303 3713
PstI C_TGCA'G
Cuts at: 362 405 1063 1748 362
Size: 43 658 685 2630
Fragments arranged by size:
2630 685 658 43
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4016
PvuII CAG'CTG
Cuts at: 1441 1801 1441
Size: 360 3656
RsrII CG'GwC_CG
Cuts at: 2211 2211
Size: 4016
SacI G_AGCT'C
Cuts at: 1030 1030
Size: 4016
SalI G'TCGA_C
Cuts at: 1053 1053
Size: 4016
SapI GCTCTTCn'nnn_
Cuts at: 2039 2249 2039
Size: 210 3806
SbfI CC_TGCA'GG
Cuts at: 1063 1063
Size: 4016
ScaI AGT'ACT
Cuts at: 142 3533 142
Size: 3391 625
SfcI C'TryA_G
Cuts at: 33 358 401 1059 1744 2737 2928 33
Size: 325 43 658 685 993 191 1121
Fragments arranged by size:
1121 993 685 658 325 191 43
SfoI GGC'GCC
Cuts at: 1448 1696 1448
Size: 248 3768
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
SmaI CCC'GGG
Cuts at: 1038 1038
Size: 4016
SmlI C'TyrA_G
Cuts at: 136 1171 2086 2578 2840 136
Size: 1035 915 492 262 1312
Fragments arranged by size:
1312 1035 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4016
SphI G_CATG'C
Cuts at: 1069 1569 1069
Size: 500 3516
StyI C'CwwG_G
Cuts at: 599 1150 1504 599
Size: 551 354 3111
Fragments arranged by size:
3111 551 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1599 1732 2191 2400 1599
Size: 133 459 209 3215
Fragments arranged by size:
3215 459 209 133
TatI w'GTAC_w
Cuts at: 140 779 3531 140
Size: 639 2752 625
Fragments arranged by size:
2752 639 625
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 3984
Tth111I GACn'n_nGTC
Cuts at: 1813 1813
Size: 4016
XbaI T'CTAG_A
Cuts at: 877 1047 877
Size: 170 3846
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1511 1511
Size: 4016
XmaI C'CCGG_G
Cuts at: 1036 1036
Size: 4016
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI