Restriction map for
pF1KE2761
(Circular) (Six-base) MAPSORT of: pF1KE2761.seq Check: 7099 from: 1 to: 5618
pF1KE2761 5618 bp
With 182 enzymes: SgfI *
October 3, 2019 17:18 ..
AccI GT'mk_AC
Cuts at: 1215 1452 1839 2656 1215
Size: 237 387 817 4177
Fragments arranged by size:
4177 817 387 237
Acc65I G'GTAC_C
Cuts at: 652 2634 652
Size: 1982 3636
AclI AA'CG_TT
Cuts at: 5458 5458
Size: 5618
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1805 3443 3875 4622 4890 1805
Size: 1638 432 747 268 2533
Fragments arranged by size:
2533 1638 747 432 268
AflIII A'CryG_T
Cuts at: 212 2970 4074 5051 212
Size: 2758 1104 977 779
Fragments arranged by size:
2758 1104 977 779
AgeI A'CCGG_T
Cuts at: 4006 4006
Size: 5618
AhdI GACnn_n'nnGTC
Cuts at: 2533 2533
Size: 5618
AlwNI CAG_nnn'CTG
Cuts at: 923 2923 4490 923
Size: 2000 1567 2051
Fragments arranged by size:
2051 2000 1567
ApaLI G'TGCA_C
Cuts at: 4388 4388
Size: 5618
ApoI r'AATT_y
Cuts at: 351 1486 2154 2622 4971 5227 351
Size: 1135 668 468 2349 256 742
Fragments arranged by size:
2349 1135 742 668 468 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5618
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5618
AvaI C'yCGr_G
Cuts at: 776 824 2638 776
Size: 48 1814 3756
Fragments arranged by size:
3756 1814 48
BamHI G'GATC_C
Cuts at: 2643 2643
Size: 5618
BanI G'GyrC_C
Cuts at: 652 1681 2634 3048 3296 3331 652
Size: 1029 953 414 248 35 2939
Fragments arranged by size:
2939 1029 953 414 248 35
BanII G_rGCy'C
Cuts at: 2167 2632 3662 2167
Size: 465 1030 4123
Fragments arranged by size:
4123 1030 465
BbeI G_GCGC'C
Cuts at: 3052 3300 3052
Size: 248 5370
BbsI GAAGACnn'nnnn_
Cuts at: 1726 2611 1726
Size: 885 4733
BbvCI CC'TCA_GC
Cuts at: 2387 2387
Size: 5618
BciVI GTATCCnnnnn_n'
Cuts at: 3516 4277 5618 3516
Size: 761 1341 3516
Fragments arranged by size:
3516 1341 761
BclI T'GATC_A
Cuts at: 1052 3138 1052
Size: 2086 3532
BfrBI ATG'CAT
Cuts at: 132 317 132
Size: 185 5433
BglII A'GATC_T
Cuts at: 233 3133 5170 233
Size: 2900 2037 681
Fragments arranged by size:
2900 2037 681
BlpI GC'TnA_GC
Cuts at: 811 1526 2730 811
Size: 715 1204 3699
Fragments arranged by size:
3699 1204 715
Bme1580I G_kGCm'C
Cuts at: 3243 3336 4392 3243
Size: 93 1056 4469
Fragments arranged by size:
4469 1056 93
BmgBI CAC'GTC
Cuts at: 1219 1837 1219
Size: 618 5000
BmrI ACTGGGnnnn_n'
Cuts at: 1717 2830 2911 3246 5390 1717
Size: 1113 81 335 2144 1945
Fragments arranged by size:
2144 1945 1113 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3985 3985
Size: 5618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1618 1618
Size: 5618
Bpu10I CC'TnA_GC
Cuts at: 2387 2387
Size: 5618
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 2794 3673 4165 4463 396
Size: 2398 879 492 298 1551
Fragments arranged by size:
2398 1551 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5322 5322
Size: 5618
BsaAI yAC'GTr
Cuts at: 1741 2971 1741
Size: 1230 4388
BsaBI GATnn'nnATC
Cuts at: 6 3132 5169 6
Size: 3126 2037 455
Fragments arranged by size:
3126 2037 455
BsaHI Gr'CG_yC
Cuts at: 3049 3297 3999 5495 3049
Size: 248 702 1496 3172
Fragments arranged by size:
3172 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2807 3328 4006 4280 4427 4838 2807
Size: 521 678 274 147 411 3587
Fragments arranged by size:
3587 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2567 2597 2567
Size: 30 5588
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 932 1356 932
Size: 424 5194
BseYI C'CCAG_C
Cuts at: 744 1536 2176 2491 3043 3801 4378 4874 744
Size: 792 640 315 552 758 577 496 1488
Fragments arranged by size:
1488 792 758 640 577 552 496 315
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2316 2316
Size: 5618
BsiEI CG_ry'CG
Cuts at: 104 3160 3206 4414 104
Size: 3056 46 1208 1308
Fragments arranged by size:
3056 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2632 3410 3600 4392 2632
Size: 778 190 792 3858
Fragments arranged by size:
3858 792 778 190
BsmI GAATG_Cn'
Cuts at: 134 1473 134
Size: 1339 4279
Bsp1286I G_dGCh'C
Cuts at: 2167 2632 3243 3336 3410 3600 3662 4392 2167
Size: 465 611 93 74 190 62 730 3393
Fragments arranged by size:
3393 730 611 465 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4838 4838
Size: 5618
BspHI T'CATG_A
Cuts at: 731 1569 4794 731
Size: 838 3225 1555
Fragments arranged by size:
3225 1555 838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2034 2668 2882 3184 3565 2034
Size: 634 214 302 381 4087
Fragments arranged by size:
4087 634 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3910 3964 5448 5613 50
Size: 3860 54 1484 165 55
Fragments arranged by size:
3860 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2231 3530 2231
Size: 1299 4319
BsrFI r'CCGG_y
Cuts at: 3616 3797 4006 3616
Size: 181 209 5228
Fragments arranged by size:
5228 209 181
BssSI C'ACGA_G
Cuts at: 581 1715 1963 3889 4247 581
Size: 1134 248 1926 358 1952
Fragments arranged by size:
1952 1926 1134 358 248
BstAPI GCAn_nnn'nTGC
Cuts at: 535 2995 535
Size: 2460 3158
BstBI TT'CG_AA
Cuts at: 126 3979 126
Size: 3853 1765
BstYI r'GATC_y
Cuts at: 1 233 2643 3110 3133 3468 3714 4726 4812
Size: 232 2410 467 23 335 246 1012 86
Cuts at: 4812 4824 5170 1
Size: 12 346 449
Fragments arranged by size:
2410 1012 467 449 346 335 246 232
86 23 12
BtgI C'CryG_G
Cuts at: 1910 2171 1910
Size: 261 5357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3568 3749 3912 3568
Size: 181 163 5274
Fragments arranged by size:
5274 181 163
BtsI GCAGTG_nn'
Cuts at: 291 1802 2861 291
Size: 1511 1059 3048
Fragments arranged by size:
3048 1511 1059
DraI TTT'AAA
Cuts at: 2617 2617
Size: 5618
DrdI GACnn_nn'nnGTC
Cuts at: 3324 4182 3324
Size: 858 4760
EaeI y'GGCC_r
Cuts at: 3203 3377 3768 3795 5140 3203
Size: 174 391 27 1345 3681
Fragments arranged by size:
3681 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3203 3203
Size: 5618
EarI CTCTTCn'nnn_
Cuts at: 263 1262 2046 3641 3851 263
Size: 999 784 1595 210 2030
Fragments arranged by size:
2030 1595 999 784 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3826 4136 4282 5428 3826
Size: 310 146 1146 4016
Fragments arranged by size:
4016 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2630 2630
Size: 5618
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1618 1805 3443 3875 4622 4890 1618
Size: 187 1638 432 747 268 2346
Fragments arranged by size:
2346 1638 747 432 268 187
EcoO109I rG'GnC_Cy
Cuts at: 601 2757 601
Size: 2156 3462
EcoRI G'AATT_C
Cuts at: 2622 2622
Size: 5618
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1134 3637 1134
Size: 2503 3115
FspI TGC'GCA
Cuts at: 3399 3399
Size: 5618
HaeII r_GCGC'y
Cuts at: 3052 3300 4322 5301 3052
Size: 248 1022 979 3369
Fragments arranged by size:
3369 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 873 905 918 950 1126 1158 1369 1401 1551
Size: 32 13 32 176 32 211 32 150
Cuts at: 1551 1583 2519 2551 3460 3490 3492 3522 873
Size: 32 936 32 909 30 2 30 2969
Fragments arranged by size:
2969 936 909 211 176 150 32 32
32 32 32 32 30 30 13 2
HincII GTy'rAC
Cuts at: 361 2142 2657 361
Size: 1781 515 3322
Fragments arranged by size:
3322 1781 515
HindIII A'AGCT_T
Cuts at: 476 1612 476
Size: 1136 4482
KasI G'GCGC_C
Cuts at: 3048 3296 3048
Size: 248 5370
KpnI G_GTAC'C
Cuts at: 656 2638 656
Size: 1982 3636
MfeI C'AATT_G
Cuts at: 1206 1206
Size: 5618
MluI A'CGCG_T
Cuts at: 5051 5051
Size: 5618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 223 572 1529 4289 4473 223
Size: 349 957 2760 184 1368
Fragments arranged by size:
2760 1368 957 349 184
MscI TGG'CCA
Cuts at: 3379 3379
Size: 5618
MslI CAynn'nnrTG
Cuts at: 196 211 1574 1915 3607 3734 196
Size: 15 1363 341 1692 127 2080
Fragments arranged by size:
2080 1692 1363 341 127 15
MspA1I CmG'CkG
Cuts at: 2330 2729 3043 3403 4416 4661 2330
Size: 399 314 360 1013 245 3287
Fragments arranged by size:
3287 1013 399 360 314 245
NaeI GCC'GGC
Cuts at: 3799 3799
Size: 5618
NarI GG'CG_CC
Cuts at: 3049 3297 3049
Size: 248 5370
NcoI C'CATG_G
Cuts at: 1910 2171 1910
Size: 261 5357
NgoMIV G'CCGG_C
Cuts at: 3797 3797
Size: 5618
NsiI A_TGCA'T
Cuts at: 134 319 134
Size: 185 5433
NspI r_CATG'y
Cuts at: 216 705 2429 2671 3171 4078 216
Size: 489 1724 242 500 907 1756
Fragments arranged by size:
1756 1724 907 500 489 242
PasI CC'CwG_GG
Cuts at: 158 1424 158
Size: 1266 4352
PciI A'CATG_T
Cuts at: 212 4074 212
Size: 3862 1756
PmeI GTTT'AAAC
Cuts at: 2617 2617
Size: 5618
PmlI CAC'GTG
Cuts at: 1741 1741
Size: 5618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4757 4789 4757
Size: 32 5586
PpuMI rG'GwC_Cy
Cuts at: 601 601
Size: 5618
PsiI TTA'TAA
Cuts at: 295 1306 1658 295
Size: 1011 352 4255
Fragments arranged by size:
4255 1011 352
PstI C_TGCA'G
Cuts at: 1758 2665 3350 1758
Size: 907 685 4026
Fragments arranged by size:
4026 907 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5618
PvuII CAG'CTG
Cuts at: 2330 3043 3403 2330
Size: 713 360 4545
Fragments arranged by size:
4545 713 360
RsrII CG'GwC_CG
Cuts at: 3813 3813
Size: 5618
SacI G_AGCT'C
Cuts at: 2632 2632
Size: 5618
SalI G'TCGA_C
Cuts at: 2655 2655
Size: 5618
SapI GCTCTTCn'nnn_
Cuts at: 3641 3851 3641
Size: 210 5408
SbfI CC_TGCA'GG
Cuts at: 2665 2665
Size: 5618
ScaI AGT'ACT
Cuts at: 1391 5135 1391
Size: 3744 1874
SfcI C'TryA_G
Cuts at: 33 1178 1453 1754 1840 2345 2661 3346 4339
Size: 1145 275 301 86 505 316 685 993
Cuts at: 4339 4530 33
Size: 191 1121
Fragments arranged by size:
1145 1121 993 685 505 316 301 275
191 86
SfoI GGC'GCC
Cuts at: 3050 3298 3050
Size: 248 5370
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5618
SmaI CCC'GGG
Cuts at: 2640 2640
Size: 5618
SmlI C'TyrA_G
Cuts at: 411 2773 3688 4180 4442 411
Size: 2362 915 492 262 1587
Fragments arranged by size:
2362 1587 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5618
SphI G_CATG'C
Cuts at: 2429 2671 3171 2429
Size: 242 500 4876
Fragments arranged by size:
4876 500 242
SspI AAT'ATT
Cuts at: 576 576
Size: 5618
StyI C'CwwG_G
Cuts at: 758 1910 2090 2171 2586 2752 3106 758
Size: 1152 180 81 415 166 354 3270
Fragments arranged by size:
3270 1152 415 354 180 166 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2353 3201 3334 3793 4002 2353
Size: 848 133 459 209 3969
Fragments arranged by size:
3969 848 459 209 133
TatI w'GTAC_w
Cuts at: 427 952 1112 1389 2011 5133 427
Size: 525 160 277 622 3122 912
Fragments arranged by size:
3122 912 622 525 277 160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1701 1733 5135 5167 1701
Size: 32 3402 32 2152
Fragments arranged by size:
3402 2152 32 32
Tth111I GACn'n_nGTC
Cuts at: 3415 3415
Size: 5618
XbaI T'CTAG_A
Cuts at: 2649 2649
Size: 5618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3113 3113
Size: 5618
XmaI C'CCGG_G
Cuts at: 2638 2638
Size: 5618
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PmeI PmlI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BglI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI NdeI NheI
NotI NruI PacI PflMI PfoI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI