Restriction map for
pF1KE2755
(Circular) (Six-base) MAPSORT of: pF1KE2755.seq Check: 9720 from: 1 to: 3512
pF1KE2755 3512 bp
With 182 enzymes: SgfI *
June 30, 2019 14:46 ..
AccI GT'mk_AC
Cuts at: 550 550
Size: 3512
Acc65I G'GTAC_C
Cuts at: 528 528
Size: 3512
AclI AA'CG_TT
Cuts at: 3352 3352
Size: 3512
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1337 1769 2516 2784 1337
Size: 432 747 268 2065
Fragments arranged by size:
2065 747 432 268
AflIII A'CryG_T
Cuts at: 864 1968 2945 864
Size: 1104 977 1431
Fragments arranged by size:
1431 1104 977
AgeI A'CCGG_T
Cuts at: 1900 1900
Size: 3512
AlwNI CAG_nnn'CTG
Cuts at: 274 817 2384 274
Size: 543 1567 1402
Fragments arranged by size:
1567 1402 543
ApaLI G'TGCA_C
Cuts at: 131 2282 131
Size: 2151 1361
ApoI r'AATT_y
Cuts at: 495 516 2865 3121 495
Size: 21 2349 256 886
Fragments arranged by size:
2349 886 256 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 3512
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3512
AvaI C'yCGr_G
Cuts at: 532 532
Size: 3512
BamHI G'GATC_C
Cuts at: 537 537
Size: 3512
BanI G'GyrC_C
Cuts at: 528 942 1190 1225 528
Size: 414 248 35 2815
Fragments arranged by size:
2815 414 248 35
BanII G_rGCy'C
Cuts at: 172 526 1556 172
Size: 354 1030 2128
Fragments arranged by size:
2128 1030 354
BbeI G_GCGC'C
Cuts at: 946 1194 946
Size: 248 3264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3478
BciVI GTATCCnnnnn_n'
Cuts at: 469 1410 2171 3512 469
Size: 941 761 1341 469
Fragments arranged by size:
1341 941 761 469
BclI T'GATC_A
Cuts at: 1032 1032
Size: 3512
BglII A'GATC_T
Cuts at: 1027 3064 1027
Size: 2037 1475
BlpI GC'TnA_GC
Cuts at: 624 624
Size: 3512
Bme1580I G_kGCm'C
Cuts at: 135 1137 1230 2286 135
Size: 1002 93 1056 1361
Fragments arranged by size:
1361 1056 1002 93
BmrI ACTGGGnnnn_n'
Cuts at: 724 805 1140 3284 724
Size: 81 335 2144 952
Fragments arranged by size:
2144 952 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1879 1879
Size: 3512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 242 138
Size: 104 3408
Bpu10I CC'TnA_GC
Cuts at: 171 171
Size: 3512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1567 2059 2357 688
Size: 879 492 298 1843
Fragments arranged by size:
1843 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 377 3216 377
Size: 2839 673
BsaAI yAC'GTr
Cuts at: 865 865
Size: 3512
BsaBI GATnn'nnATC
Cuts at: 6 1026 3063 6
Size: 1020 2037 455
Fragments arranged by size:
2037 1020 455
BsaHI Gr'CG_yC
Cuts at: 943 1191 1893 3389 943
Size: 248 702 1496 1066
Fragments arranged by size:
1496 1066 702 248
BsaWI w'CCGG_w
Cuts at: 701 1222 1900 2174 2321 2732 701
Size: 521 678 274 147 411 1481
Fragments arranged by size:
1481 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 937 1695 2272 2768 937
Size: 758 577 496 1681
Fragments arranged by size:
1681 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 3512
BsiEI CG_ry'CG
Cuts at: 104 1054 1100 2308 104
Size: 950 46 1208 1308
Fragments arranged by size:
1308 1208 950 46
BsiHKAI G_wGCw'C
Cuts at: 135 172 526 1304 1494 2286 135
Size: 37 354 778 190 792 1361
Fragments arranged by size:
1361 792 778 354 190 37
Bsp1286I G_dGCh'C
Cuts at: 135 172 526 1137 1230 1304 1494 1556 2286
Size: 37 354 611 93 74 190 62 730
Cuts at: 2286 135
Size: 1361
Fragments arranged by size:
1361 730 611 354 190 93 74 62
37
BspEI T'CCGG_A
Cuts at: 2732 2732
Size: 3512
BspHI T'CATG_A
Cuts at: 2688 2688
Size: 3512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 562 776 1078 1459 562
Size: 214 302 381 2615
Fragments arranged by size:
2615 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1804 1858 3342 3507 50
Size: 1754 54 1484 165 55
Fragments arranged by size:
1754 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1424 1424
Size: 3512
BsrFI r'CCGG_y
Cuts at: 485 1510 1691 1900 485
Size: 1025 181 209 2097
Fragments arranged by size:
2097 1025 209 181
BssSI C'ACGA_G
Cuts at: 1783 2141 1783
Size: 358 3154
BstAPI GCAn_nnn'nTGC
Cuts at: 889 889
Size: 3512
BstBI TT'CG_AA
Cuts at: 1873 1873
Size: 3512
BstYI r'GATC_y
Cuts at: 1 537 1004 1027 1362 1608 2620 2706 2718
Size: 536 467 23 335 246 1012 86 12
Cuts at: 2718 3064 1
Size: 346 449
Fragments arranged by size:
1012 536 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 464 464
Size: 3512
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1462 1643 1806 1462
Size: 181 163 3168
Fragments arranged by size:
3168 181 163
BtsI GCAGTG_nn'
Cuts at: 755 755
Size: 3512
DraI TTT'AAA
Cuts at: 511 511
Size: 3512
DrdI GACnn_nn'nnGTC
Cuts at: 1218 2076 1218
Size: 858 2654
EaeI y'GGCC_r
Cuts at: 1097 1271 1662 1689 3034 1097
Size: 174 391 27 1345 1575
Fragments arranged by size:
1575 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1097 1097
Size: 3512
EarI CTCTTCn'nnn_
Cuts at: 254 1535 1745 254
Size: 1281 210 2021
Fragments arranged by size:
2021 1281 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1720 2030 2176 3322 1720
Size: 310 146 1146 1910
Fragments arranged by size:
1910 1146 310 146
EcoICRI GAG'CTC
Cuts at: 170 524 170
Size: 354 3158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 242 1337 1769 2516 2784 138
Size: 104 1095 432 747 268 866
Fragments arranged by size:
1095 866 747 432 268 104
EcoO109I rG'GnC_Cy
Cuts at: 651 651
Size: 3512
EcoRI G'AATT_C
Cuts at: 516 516
Size: 3512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1531 1531
Size: 3512
FspI TGC'GCA
Cuts at: 1293 1293
Size: 3512
HaeII r_GCGC'y
Cuts at: 946 1194 2216 3195 946
Size: 248 1022 979 1263
Fragments arranged by size:
1263 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 223 255 373 405 443 475 1354 1384 1386
Size: 32 118 32 38 32 879 30 2
Cuts at: 1386 1416 223
Size: 30 2319
Fragments arranged by size:
2319 879 118 38 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 242 551 242
Size: 309 3203
KasI G'GCGC_C
Cuts at: 942 1190 942
Size: 248 3264
KpnI G_GTAC'C
Cuts at: 532 532
Size: 3512
MluI A'CGCG_T
Cuts at: 2945 2945
Size: 3512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2183 2367 2183
Size: 184 3328
MscI TGG'CCA
Cuts at: 1273 1273
Size: 3512
MslI CAynn'nnrTG
Cuts at: 232 1501 1628 232
Size: 1269 127 2116
Fragments arranged by size:
2116 1269 127
MspA1I CmG'CkG
Cuts at: 394 623 937 1297 2310 2555 394
Size: 229 314 360 1013 245 1351
Fragments arranged by size:
1351 1013 360 314 245 229
NaeI GCC'GGC
Cuts at: 1693 1693
Size: 3512
NarI GG'CG_CC
Cuts at: 943 1191 943
Size: 248 3264
NcoI C'CATG_G
Cuts at: 464 464
Size: 3512
NgoMIV G'CCGG_C
Cuts at: 1691 1691
Size: 3512
NspI r_CATG'y
Cuts at: 565 1065 1972 565
Size: 500 907 2105
Fragments arranged by size:
2105 907 500
PciI A'CATG_T
Cuts at: 1968 1968
Size: 3512
PmeI GTTT'AAAC
Cuts at: 511 511
Size: 3512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2651 2683 2651
Size: 32 3480
PstI C_TGCA'G
Cuts at: 559 1244 559
Size: 685 2827
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3512
PvuII CAG'CTG
Cuts at: 394 937 1297 394
Size: 543 360 2609
Fragments arranged by size:
2609 543 360
RsrII CG'GwC_CG
Cuts at: 1707 1707
Size: 3512
SacI G_AGCT'C
Cuts at: 172 526 172
Size: 354 3158
SalI G'TCGA_C
Cuts at: 549 549
Size: 3512
SapI GCTCTTCn'nnn_
Cuts at: 1535 1745 1535
Size: 210 3302
SbfI CC_TGCA'GG
Cuts at: 559 559
Size: 3512
ScaI AGT'ACT
Cuts at: 3029 3029
Size: 3512
SfcI C'TryA_G
Cuts at: 33 401 555 1240 2233 2424 33
Size: 368 154 685 993 191 1121
Fragments arranged by size:
1121 993 685 368 191 154
SfoI GGC'GCC
Cuts at: 944 1192 944
Size: 248 3264
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3512
SmaI CCC'GGG
Cuts at: 534 534
Size: 3512
SmlI C'TyrA_G
Cuts at: 667 1582 2074 2336 667
Size: 915 492 262 1843
Fragments arranged by size:
1843 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3512
SphI G_CATG'C
Cuts at: 565 1065 565
Size: 500 3012
StuI AGG'CCT
Cuts at: 211 211
Size: 3512
StyI C'CwwG_G
Cuts at: 464 646 1000 464
Size: 182 354 2976
Fragments arranged by size:
2976 354 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 261 1095 1228 1687 1896 261
Size: 834 133 459 209 1877
Fragments arranged by size:
1877 834 459 209 133
TatI w'GTAC_w
Cuts at: 3027 3027
Size: 3512
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 125 157 3029 3061 125
Size: 32 2872 32 576
Fragments arranged by size:
2872 576 32 32
Tth111I GACn'n_nGTC
Cuts at: 1309 1309
Size: 3512
XbaI T'CTAG_A
Cuts at: 543 543
Size: 3512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1007 1007
Size: 3512
XmaI C'CCGG_G
Cuts at: 532 532
Size: 3512
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI