Restriction map for
pF1KE2721
(Circular) (Six-base) MAPSORT of: pF1KE2721.seq Check: 5367 from: 1 to: 3704
pF1KE2721 3704 bp
With 182 enzymes: SgfI *
June 6, 2018 17:50 ..
AccI GT'mk_AC
Cuts at: 742 742
Size: 3704
Acc65I G'GTAC_C
Cuts at: 720 720
Size: 3704
AclI AA'CG_TT
Cuts at: 3544 3544
Size: 3704
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 192 670 1529 1961 2708 2976 190
Size: 2 478 859 432 747 268 918
Fragments arranged by size:
918 859 747 478 432 268 2
AflIII A'CryG_T
Cuts at: 1056 2160 3137 1056
Size: 1104 977 1623
Fragments arranged by size:
1623 1104 977
AgeI A'CCGG_T
Cuts at: 2092 2092
Size: 3704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 418 418
Size: 3704
AlwNI CAG_nnn'CTG
Cuts at: 507 1009 2576 507
Size: 502 1567 1635
Fragments arranged by size:
1635 1567 502
ApaI G_GGCC'C
Cuts at: 118 118
Size: 3704
ApaLI G'TGCA_C
Cuts at: 2474 2474
Size: 3704
ApoI r'AATT_y
Cuts at: 708 3057 3313 708
Size: 2349 256 1099
Fragments arranged by size:
2349 1099 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3704
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
AvaI C'yCGr_G
Cuts at: 234 336 724 234
Size: 102 388 3214
Fragments arranged by size:
3214 388 102
BamHI G'GATC_C
Cuts at: 729 729
Size: 3704
BanI G'GyrC_C
Cuts at: 694 720 1134 1382 1417 694
Size: 26 414 248 35 2981
Fragments arranged by size:
2981 414 248 35 26
BanII G_rGCy'C
Cuts at: 118 593 718 1748 118
Size: 475 125 1030 2074
Fragments arranged by size:
2074 1030 475 125
BbeI G_GCGC'C
Cuts at: 698 1138 1386 698
Size: 440 248 3016
Fragments arranged by size:
3016 440 248
BciVI GTATCCnnnnn_n'
Cuts at: 1602 2363 3704 1602
Size: 761 1341 1602
Fragments arranged by size:
1602 1341 761
BclI T'GATC_A
Cuts at: 1224 1224
Size: 3704
BglI GCCn_nnn'nGGC
Cuts at: 382 382
Size: 3704
BglII A'GATC_T
Cuts at: 1219 3256 1219
Size: 2037 1667
BlpI GC'TnA_GC
Cuts at: 133 816 133
Size: 683 3021
Bme1580I G_kGCm'C
Cuts at: 118 1329 1422 2478 118
Size: 1211 93 1056 1344
Fragments arranged by size:
1344 1211 1056 93
BmgBI CAC'GTC
Cuts at: 411 411
Size: 3704
BmrI ACTGGGnnnn_n'
Cuts at: 916 997 1332 3476 916
Size: 81 335 2144 1144
Fragments arranged by size:
2144 1144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 678 2071 678
Size: 1393 2311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 517 166
Size: 351 3353
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1759 2251 2549 880
Size: 879 492 298 2035
Fragments arranged by size:
2035 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3408 3408
Size: 3704
BsaAI yAC'GTr
Cuts at: 492 1057 492
Size: 565 3139
BsaBI GATnn'nnATC
Cuts at: 6 1218 3255 6
Size: 1212 2037 455
Fragments arranged by size:
2037 1212 455
BsaHI Gr'CG_yC
Cuts at: 695 1135 1383 2085 3581 695
Size: 440 248 702 1496 818
Fragments arranged by size:
1496 818 702 440 248
BsaWI w'CCGG_w
Cuts at: 893 1414 2092 2366 2513 2924 893
Size: 521 678 274 147 411 1673
Fragments arranged by size:
1673 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 262 547 262
Size: 285 3419
BseYI C'CCAG_C
Cuts at: 151 1129 1887 2464 2960 151
Size: 978 758 577 496 895
Fragments arranged by size:
978 895 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 400 472 493 391
Size: 9 72 21 3602
Fragments arranged by size:
3602 72 21 9
BsiEI CG_ry'CG
Cuts at: 104 1246 1292 2500 104
Size: 1142 46 1208 1308
Fragments arranged by size:
1308 1208 1142 46
BsiHKAI G_wGCw'C
Cuts at: 163 191 718 1496 1686 2478 163
Size: 28 527 778 190 792 1389
Fragments arranged by size:
1389 792 778 527 190 28
Bsp1286I G_dGCh'C
Cuts at: 118 163 191 593 718 1329 1422 1496 1686
Size: 45 28 402 125 611 93 74 190
Cuts at: 1686 1748 2478 118
Size: 62 730 1344
Fragments arranged by size:
1344 730 611 402 190 125 93 74
62 45 28
BspEI T'CCGG_A
Cuts at: 2924 2924
Size: 3704
BspHI T'CATG_A
Cuts at: 2880 2880
Size: 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 425 754 968 1270 1651 425
Size: 329 214 302 381 2478
Fragments arranged by size:
2478 381 329 302 214
BsrBI CCG'CTC
Cuts at: 50 137 1996 2050 3534 3699 50
Size: 87 1859 54 1484 165 55
Fragments arranged by size:
1859 1484 165 87 55 54
BsrDI GCAATG_nn'
Cuts at: 1616 1616
Size: 3704
BsrFI r'CCGG_y
Cuts at: 307 691 1702 1883 2092 307
Size: 384 1011 181 209 1919
Fragments arranged by size:
1919 1011 384 209 181
BssSI C'ACGA_G
Cuts at: 225 354 1975 2333 225
Size: 129 1621 358 1596
Fragments arranged by size:
1621 1596 358 129
BstAPI GCAn_nnn'nTGC
Cuts at: 378 426 507 1081 378
Size: 48 81 574 3001
Fragments arranged by size:
3001 574 81 48
BstBI TT'CG_AA
Cuts at: 2065 2065
Size: 3704
BstXI CCAn_nnnn'nTGG
Cuts at: 126 126
Size: 3704
BstYI r'GATC_y
Cuts at: 1 729 1196 1219 1554 1800 2812 2898 2910
Size: 728 467 23 335 246 1012 86 12
Cuts at: 2910 3256 1
Size: 346 449
Fragments arranged by size:
1012 728 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1654 1835 1998 1654
Size: 181 163 3360
Fragments arranged by size:
3360 181 163
BtsI GCAGTG_nn'
Cuts at: 484 947 484
Size: 463 3241
DraI TTT'AAA
Cuts at: 703 703
Size: 3704
DrdI GACnn_nn'nnGTC
Cuts at: 1410 2268 1410
Size: 858 2846
EaeI y'GGCC_r
Cuts at: 1289 1463 1854 1881 3226 1289
Size: 174 391 27 1345 1767
Fragments arranged by size:
1767 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1289 1289
Size: 3704
EarI CTCTTCn'nnn_
Cuts at: 151 166 1727 1937 151
Size: 15 1561 210 1918
Fragments arranged by size:
1918 1561 210 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1912 2222 2368 3514 1912
Size: 310 146 1146 2102
Fragments arranged by size:
2102 1146 310 146
EcoICRI GAG'CTC
Cuts at: 716 716
Size: 3704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 190 192 517 670 1529 1961 2708 2976
Size: 24 2 325 153 859 432 747 268
Cuts at: 2976 166
Size: 894
Fragments arranged by size:
894 859 747 432 325 268 153 24
2
EcoNI CCTnn'n_nnAGG
Cuts at: 631 631
Size: 3704
EcoO109I rG'GnC_Cy
Cuts at: 115 843 115
Size: 728 2976
EcoRI G'AATT_C
Cuts at: 708 708
Size: 3704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1723 1723
Size: 3704
FspI TGC'GCA
Cuts at: 1485 1485
Size: 3704
HaeII r_GCGC'y
Cuts at: 661 698 1138 1386 2408 3387 661
Size: 37 440 248 1022 979 978
Fragments arranged by size:
1022 979 978 440 248 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1546 1576 1578 1608 1546
Size: 30 2 30 3642
Fragments arranged by size:
3642 30 30 2
HincII GTy'rAC
Cuts at: 743 743
Size: 3704
KasI G'GCGC_C
Cuts at: 694 1134 1382 694
Size: 440 248 3016
Fragments arranged by size:
3016 440 248
KpnI G_GTAC'C
Cuts at: 724 724
Size: 3704
MluI A'CGCG_T
Cuts at: 3137 3137
Size: 3704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2375 2559 2375
Size: 184 3520
MscI TGG'CCA
Cuts at: 1465 1465
Size: 3704
MslI CAynn'nnrTG
Cuts at: 400 469 1693 1820 400
Size: 69 1224 127 2284
Fragments arranged by size:
2284 1224 127 69
MspA1I CmG'CkG
Cuts at: 194 504 815 1129 1489 2502 2747 194
Size: 310 311 314 360 1013 245 1151
Fragments arranged by size:
1151 1013 360 314 311 310 245
NaeI GCC'GGC
Cuts at: 309 1885 309
Size: 1576 2128
NarI GG'CG_CC
Cuts at: 695 1135 1383 695
Size: 440 248 3016
Fragments arranged by size:
3016 440 248
NgoMIV G'CCGG_C
Cuts at: 307 1883 307
Size: 1576 2128
NspI r_CATG'y
Cuts at: 328 757 1257 2164 328
Size: 429 500 907 1868
Fragments arranged by size:
1868 907 500 429
PciI A'CATG_T
Cuts at: 2160 2160
Size: 3704
PfoI T'CCnGG_A
Cuts at: 182 182
Size: 3704
PmeI GTTT'AAAC
Cuts at: 703 703
Size: 3704
PmlI CAC'GTG
Cuts at: 492 492
Size: 3704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2843 2875 2843
Size: 32 3672
PspOMI G'GGCC_C
Cuts at: 114 114
Size: 3704
PstI C_TGCA'G
Cuts at: 422 751 1436 422
Size: 329 685 2690
Fragments arranged by size:
2690 685 329
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3704
PvuII CAG'CTG
Cuts at: 1129 1489 1129
Size: 360 3344
RsrII CG'GwC_CG
Cuts at: 1899 1899
Size: 3704
SacI G_AGCT'C
Cuts at: 718 718
Size: 3704
SalI G'TCGA_C
Cuts at: 741 741
Size: 3704
SapI GCTCTTCn'nnn_
Cuts at: 151 1727 1937 151
Size: 1576 210 1918
Fragments arranged by size:
1918 1576 210
SbfI CC_TGCA'GG
Cuts at: 422 751 422
Size: 329 3375
ScaI AGT'ACT
Cuts at: 3221 3221
Size: 3704
SfcI C'TryA_G
Cuts at: 33 418 747 1432 2425 2616 33
Size: 385 329 685 993 191 1121
Fragments arranged by size:
1121 993 685 385 329 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 382 382
Size: 3704
SfoI GGC'GCC
Cuts at: 696 1136 1384 696
Size: 440 248 3016
Fragments arranged by size:
3016 440 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
SmaI CCC'GGG
Cuts at: 726 726
Size: 3704
SmlI C'TyrA_G
Cuts at: 859 1774 2266 2528 859
Size: 915 492 262 2035
Fragments arranged by size:
2035 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3704
SphI G_CATG'C
Cuts at: 328 757 1257 328
Size: 429 500 2775
Fragments arranged by size:
2775 500 429
StuI AGG'CCT
Cuts at: 123 637 123
Size: 514 3190
StyI C'CwwG_G
Cuts at: 838 1192 838
Size: 354 3350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1287 1420 1879 2088 1287
Size: 133 459 209 2903
Fragments arranged by size:
2903 459 209 133
TatI w'GTAC_w
Cuts at: 3219 3219
Size: 3704
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 3672
Tth111I GACn'n_nGTC
Cuts at: 1501 1501
Size: 3704
XbaI T'CTAG_A
Cuts at: 735 735
Size: 3704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1199 1199
Size: 3704
XmaI C'CCGG_G
Cuts at: 724 724
Size: 3704
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PmlI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI