Restriction map for
pF1KE2690
(Circular) (Six-base) MAPSORT of: pF1KE2690.seq Check: 1844 from: 1 to: 4742
pF1KE2690 4742 bp
With 182 enzymes: SgfI *
June 2, 2017 11:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1628 1628
Size: 4742
AccI GT'mk_AC
Cuts at: 1780 1780
Size: 4742
Acc65I G'GTAC_C
Cuts at: 1758 1758
Size: 4742
AclI AA'CG_TT
Cuts at: 4582 4582
Size: 4742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2999 3746 4014 2567
Size: 432 747 268 3295
Fragments arranged by size:
3295 747 432 268
AflIII A'CryG_T
Cuts at: 2094 3198 4175 2094
Size: 1104 977 2661
Fragments arranged by size:
2661 1104 977
AgeI A'CCGG_T
Cuts at: 3130 3130
Size: 4742
AhdI GACnn_n'nnGTC
Cuts at: 1650 1650
Size: 4742
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 572 572
Size: 4742
AlwNI CAG_nnn'CTG
Cuts at: 176 921 2047 3614 176
Size: 745 1126 1567 1304
Fragments arranged by size:
1567 1304 1126 745
ApaLI G'TGCA_C
Cuts at: 3512 3512
Size: 4742
ApoI r'AATT_y
Cuts at: 1746 4095 4351 1746
Size: 2349 256 2137
Fragments arranged by size:
2349 2137 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4742
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
AvaI C'yCGr_G
Cuts at: 882 1260 1468 1762 882
Size: 378 208 294 3862
Fragments arranged by size:
3862 378 294 208
AvrII C'CTAG_G
Cuts at: 1002 1002
Size: 4742
BamHI G'GATC_C
Cuts at: 459 815 1256 1767 459
Size: 356 441 511 3434
Fragments arranged by size:
3434 511 441 356
BanI G'GyrC_C
Cuts at: 1758 2172 2420 2455 1758
Size: 414 248 35 4045
Fragments arranged by size:
4045 414 248 35
BanII G_rGCy'C
Cuts at: 992 1244 1670 1756 2786 992
Size: 252 426 86 1030 2948
Fragments arranged by size:
2948 1030 426 252 86
BbeI G_GCGC'C
Cuts at: 2176 2424 2176
Size: 248 4494
BbsI GAAGACnn'nnnn_
Cuts at: 195 1709 195
Size: 1514 3228
BciVI GTATCCnnnnn_n'
Cuts at: 2640 3401 4742 2640
Size: 761 1341 2640
Fragments arranged by size:
2640 1341 761
BclI T'GATC_A
Cuts at: 2262 2262
Size: 4742
BglI GCCn_nnn'nGGC
Cuts at: 1165 1165
Size: 4742
BglII A'GATC_T
Cuts at: 2257 4294 2257
Size: 2037 2705
BlpI GC'TnA_GC
Cuts at: 922 1854 922
Size: 932 3810
Bme1580I G_kGCm'C
Cuts at: 572 1161 2367 2460 3516 572
Size: 589 1206 93 1056 1798
Fragments arranged by size:
1798 1206 1056 589 93
BmgBI CAC'GTC
Cuts at: 201 753 1122 201
Size: 552 369 3821
Fragments arranged by size:
3821 552 369
BmrI ACTGGGnnnn_n'
Cuts at: 441 521 1954 2035 2370 4514 441
Size: 80 1433 81 335 2144 669
Fragments arranged by size:
2144 1433 669 335 81 80
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3109 3109
Size: 4742
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1536 1536
Size: 4742
Bpu10I CC'TnA_GC
Cuts at: 220 220
Size: 4742
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1918 2797 3289 3587 1918
Size: 879 492 298 3073
Fragments arranged by size:
3073 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 790 1363 4446 790
Size: 573 3083 1086
Fragments arranged by size:
3083 1086 573
BsaAI yAC'GTr
Cuts at: 1077 1704 2095 1077
Size: 627 391 3724
Fragments arranged by size:
3724 627 391
BsaBI GATnn'nnATC
Cuts at: 6 2256 4293 6
Size: 2250 2037 455
Fragments arranged by size:
2250 2037 455
BsaHI Gr'CG_yC
Cuts at: 2173 2421 3123 4619 2173
Size: 248 702 1496 2296
Fragments arranged by size:
2296 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1931 2452 3130 3404 3551 3962 1931
Size: 521 678 274 147 411 2711
Fragments arranged by size:
2711 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 787 817 787
Size: 30 4712
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 808 856 145
Size: 663 48 4031
Fragments arranged by size:
4031 663 48
BseYI C'CCAG_C
Cuts at: 192 436 450 565 864 1012 1018 1024 1139
Size: 244 14 115 299 148 6 6 115
Cuts at: 1139 2167 2925 3502 3998 192
Size: 1028 758 577 496 936
Fragments arranged by size:
1028 936 758 577 496 299 244 148
115 115 14 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 491 775 844 1152 1684 412
Size: 79 284 69 308 532 3470
Fragments arranged by size:
3470 532 308 284 79 69
BsiEI CG_ry'CG
Cuts at: 104 251 2284 2330 3538 104
Size: 147 2033 46 1208 1308
Fragments arranged by size:
2033 1308 1208 147 46
BsiHKAI G_wGCw'C
Cuts at: 473 1244 1756 2534 2724 3516 473
Size: 771 512 778 190 792 1699
Fragments arranged by size:
1699 792 778 771 512 190
Bsp1286I G_dGCh'C
Cuts at: 473 572 992 1161 1244 1670 1756 2367 2460
Size: 99 420 169 83 426 86 611 93
Cuts at: 2460 2534 2724 2786 3516 473
Size: 74 190 62 730 1699
Fragments arranged by size:
1699 730 611 426 420 190 169 99
93 86 83 74 62
BspEI T'CCGG_A
Cuts at: 3962 3962
Size: 4742
BspHI T'CATG_A
Cuts at: 3918 3918
Size: 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 434 1628 1655 1792 2006 2308 2689 434
Size: 1194 27 137 214 302 381 2487
Fragments arranged by size:
2487 1194 381 302 214 137 27
BsrBI CCG'CTC
Cuts at: 50 404 3034 3088 4572 4737 50
Size: 354 2630 54 1484 165 55
Fragments arranged by size:
2630 1484 354 165 55 54
BsrDI GCAATG_nn'
Cuts at: 735 2654 735
Size: 1919 2823
BsrFI r'CCGG_y
Cuts at: 353 497 2740 2921 3130 353
Size: 144 2243 181 209 1965
Fragments arranged by size:
2243 1965 209 181 144
BsrGI T'GTAC_A
Cuts at: 1106 1106
Size: 4742
BssHII G'CGCG_C
Cuts at: 486 1276 486
Size: 790 3952
BssSI C'ACGA_G
Cuts at: 1194 3013 3371 1194
Size: 1819 358 2565
Fragments arranged by size:
2565 1819 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2119 2119
Size: 4742
BstBI TT'CG_AA
Cuts at: 3103 3103
Size: 4742
BstXI CCAn_nnnn'nTGG
Cuts at: 619 941 619
Size: 322 4420
BstYI r'GATC_y
Cuts at: 1 459 815 827 1256 1472 1767 2234 2257
Size: 458 356 12 429 216 295 467 23
Cuts at: 2257 2592 2838 3850 3936 3948 4294 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 458 449 429 356 346 335
295 246 216 86 23 12 12
Bsu36I CC'TnA_GG
Cuts at: 120 120
Size: 4742
BtgI C'CryG_G
Cuts at: 251 606 1247 1373 251
Size: 355 641 126 3620
Fragments arranged by size:
3620 641 355 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1302 2692 2873 3036 1302
Size: 1390 181 163 3008
Fragments arranged by size:
3008 1390 181 163
BtsI GCAGTG_nn'
Cuts at: 394 1985 394
Size: 1591 3151
ClaI AT'CG_AT
Cuts at: 857 857
Size: 4742
DraI TTT'AAA
Cuts at: 1741 1741
Size: 4742
DraIII CAC_nnn'GTG
Cuts at: 380 380
Size: 4742
DrdI GACnn_nn'nnGTC
Cuts at: 2448 3306 2448
Size: 858 3884
EaeI y'GGCC_r
Cuts at: 142 248 266 609 1166 2327 2501 2892 2919
Size: 106 18 343 557 1161 174 391 27
Cuts at: 2919 4264 142
Size: 1345 620
Fragments arranged by size:
1345 1161 620 557 391 343 174 106
27 18
EagI C'GGCC_G
Cuts at: 248 2327 248
Size: 2079 2663
EarI CTCTTCn'nnn_
Cuts at: 2765 2975 2765
Size: 210 4532
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1594 2950 3260 3406 4552 1594
Size: 1356 310 146 1146 1784
Fragments arranged by size:
1784 1356 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1242 1754 1242
Size: 512 4230
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1536 2567 2999 3746 4014 1536
Size: 1031 432 747 268 2264
Fragments arranged by size:
2264 1031 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 280 1489 280
Size: 1209 3533
EcoO109I rG'GnC_Cy
Cuts at: 1356 1509 1881 1356
Size: 153 372 4217
Fragments arranged by size:
4217 372 153
EcoRI G'AATT_C
Cuts at: 1746 1746
Size: 4742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4742
FseI GG_CCGG'CC
Cuts at: 501 501
Size: 4742
FspI TGC'GCA
Cuts at: 2523 2523
Size: 4742
HaeII r_GCGC'y
Cuts at: 686 2176 2424 3446 4425 686
Size: 1490 248 1022 979 1003
Fragments arranged by size:
1490 1022 1003 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 812 844 1493 1525 1636 1668 2584 2614 2616
Size: 32 649 32 111 32 916 30 2
Cuts at: 2616 2646 812
Size: 30 2908
Fragments arranged by size:
2908 916 649 111 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1781 1781
Size: 4742
KasI G'GCGC_C
Cuts at: 2172 2420 2172
Size: 248 4494
KpnI G_GTAC'C
Cuts at: 1762 1762
Size: 4742
MluI A'CGCG_T
Cuts at: 4175 4175
Size: 4742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3413 3597 3413
Size: 184 4558
MscI TGG'CCA
Cuts at: 268 611 2503 268
Size: 343 1892 2507
Fragments arranged by size:
2507 1892 343
MslI CAynn'nnrTG
Cuts at: 160 652 2731 2858 160
Size: 492 2079 127 2044
Fragments arranged by size:
2079 2044 492 127
MspA1I CmG'CkG
Cuts at: 247 864 921 1853 2167 2527 3540 3785 247
Size: 617 57 932 314 360 1013 245 1204
Fragments arranged by size:
1204 1013 932 617 360 314 245 57
NaeI GCC'GGC
Cuts at: 499 2923 499
Size: 2424 2318
NarI GG'CG_CC
Cuts at: 2173 2421 2173
Size: 248 4494
NcoI C'CATG_G
Cuts at: 1247 1247
Size: 4742
NgoMIV G'CCGG_C
Cuts at: 497 2921 497
Size: 2424 2318
NspI r_CATG'y
Cuts at: 588 1113 1692 1795 2295 3202 588
Size: 525 579 103 500 907 2128
Fragments arranged by size:
2128 907 579 525 500 103
PasI CC'CwG_GG
Cuts at: 492 941 492
Size: 449 4293
PciI A'CATG_T
Cuts at: 3198 3198
Size: 4742
PfoI T'CCnGG_A
Cuts at: 1682 1682
Size: 4742
PmeI GTTT'AAAC
Cuts at: 1741 1741
Size: 4742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 4710
PpuMI rG'GwC_Cy
Cuts at: 1509 1509
Size: 4742
PstI C_TGCA'G
Cuts at: 1789 2474 1789
Size: 685 4057
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4742
PvuII CAG'CTG
Cuts at: 864 921 2167 2527 864
Size: 57 1246 360 3079
Fragments arranged by size:
3079 1246 360 57
RsrII CG'GwC_CG
Cuts at: 2937 2937
Size: 4742
SacI G_AGCT'C
Cuts at: 1244 1756 1244
Size: 512 4230
SalI G'TCGA_C
Cuts at: 1779 1779
Size: 4742
SapI GCTCTTCn'nnn_
Cuts at: 2765 2975 2765
Size: 210 4532
SbfI CC_TGCA'GG
Cuts at: 1789 1789
Size: 4742
ScaI AGT'ACT
Cuts at: 4259 4259
Size: 4742
SexAI A'CCwGG_T
Cuts at: 281 281
Size: 4742
SfcI C'TryA_G
Cuts at: 33 306 1785 2470 3463 3654 33
Size: 273 1479 685 993 191 1121
Fragments arranged by size:
1479 1121 993 685 273 191
SfoI GGC'GCC
Cuts at: 2174 2422 2174
Size: 248 4494
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
SmaI CCC'GGG
Cuts at: 884 1764 884
Size: 880 3862
SmlI C'TyrA_G
Cuts at: 1468 1897 2812 3304 3566 1468
Size: 429 915 492 262 2644
Fragments arranged by size:
2644 915 492 429 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4742
SphI G_CATG'C
Cuts at: 1795 2295 1795
Size: 500 4242
StuI AGG'CCT
Cuts at: 1312 1390 1312
Size: 78 4664
StyI C'CwwG_G
Cuts at: 1002 1235 1247 1876 2230 1002
Size: 233 12 629 354 3514
Fragments arranged by size:
3514 629 354 233 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2325 2458 2917 3126 2325
Size: 133 459 209 3941
Fragments arranged by size:
3941 459 209 133
TatI w'GTAC_w
Cuts at: 1106 1145 4257 1106
Size: 39 3112 1591
Fragments arranged by size:
3112 1591 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4259 4291 4259
Size: 32 4710
Tth111I GACn'n_nGTC
Cuts at: 1507 2539 1507
Size: 1032 3710
XbaI T'CTAG_A
Cuts at: 1641 1773 1641
Size: 132 4610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 540 2237 540
Size: 1697 3045
XhoI C'TCGA_G
Cuts at: 1468 1468
Size: 4742
XmaI C'CCGG_G
Cuts at: 882 1762 882
Size: 880 3862
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI ApaI AscI BaeI
BbvCI BcgI BfrBI BmtI BsiWI BsmI BsmBI BstEII
BstZ17I CspCI EcoRV FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XmnI ZraI