Restriction map for
pF1KE2678
(Circular) (Six-base) MAPSORT of: pF1KE2678.seq Check: 6290 from: 1 to: 7565
pF1KE2678 7565 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AatII G_ACGT'C
Cuts at: 3928 3928
Size: 7565
AccI GT'mk_AC
Cuts at: 1676 3554 4603 1676
Size: 1878 1049 4638
Fragments arranged by size:
4638 1878 1049
Acc65I G'GTAC_C
Cuts at: 300 3684 4581 300
Size: 3384 897 3284
Fragments arranged by size:
3384 3284 897
AclI AA'CG_TT
Cuts at: 3013 7405 3013
Size: 4392 3173
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1143 1322 1830 2967 3052 3394 3459 4308 5390
Size: 179 508 1137 85 342 65 849 1082
Cuts at: 5390 5822 6569 6837 1143
Size: 432 747 268 1871
Fragments arranged by size:
1871 1137 1082 849 747 508 432 342
268 179 85 65
AfeI AGC'GCT
Cuts at: 1773 3469 1773
Size: 1696 5869
AflII C'TTAA_G
Cuts at: 1501 1501
Size: 7565
AflIII A'CryG_T
Cuts at: 725 869 2576 3044 4121 4917 6021 6998 725
Size: 144 1707 468 1077 796 1104 977 1292
Fragments arranged by size:
1707 1292 1104 1077 977 796 468 144
AgeI A'CCGG_T
Cuts at: 3731 5953 3731
Size: 2222 5343
AhdI GACnn_n'nnGTC
Cuts at: 1029 1029
Size: 7565
AleI CACnn'nnGTG
Cuts at: 3790 4027 3790
Size: 237 7328
AlwNI CAG_nnn'CTG
Cuts at: 916 1300 3894 4870 6437 916
Size: 384 2594 976 1567 2044
Fragments arranged by size:
2594 2044 1567 976 384
ApaI G_GGCC'C
Cuts at: 3623 4377 3623
Size: 754 6811
ApaLI G'TGCA_C
Cuts at: 6335 6335
Size: 7565
ApoI r'AATT_y
Cuts at: 1149 1508 3229 4569 6918 7174 1149
Size: 359 1721 1340 2349 256 1540
Fragments arranged by size:
2349 1721 1540 1340 359 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7565
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7565
AvaI C'yCGr_G
Cuts at: 305 3807 4585 305
Size: 3502 778 3285
Fragments arranged by size:
3502 3285 778
BamHI G'GATC_C
Cuts at: 4590 4590
Size: 7565
BanI G'GyrC_C
Cuts at: 300 1347 1868 3684 4581 4995 5243 5278 300
Size: 1047 521 1816 897 414 248 35 2587
Fragments arranged by size:
2587 1816 1047 897 521 414 248 35
BanII G_rGCy'C
Cuts at: 186 202 496 938 1058 1642 3116 3623 3961
Size: 16 294 442 120 584 1474 507 338
Cuts at: 3961 4111 4377 4579 5609 186
Size: 150 266 202 1030 2142
Fragments arranged by size:
2142 1474 1030 584 507 442 338 294
266 202 150 120 16
BbeI G_GCGC'C
Cuts at: 4999 5247 4999
Size: 248 7317
BbsI GAAGACnn'nnnn_
Cuts at: 2562 2682 2562
Size: 120 7445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3316 3350 3316
Size: 34 7531
BciVI GTATCCnnnnn_n'
Cuts at: 894 5463 6224 7565 894
Size: 4569 761 1341 894
Fragments arranged by size:
4569 1341 894 761
BclI T'GATC_A
Cuts at: 1124 5085 1124
Size: 3961 3604
BfrBI ATG'CAT
Cuts at: 2214 2257 2214
Size: 43 7522
BglI GCCn_nnn'nGGC
Cuts at: 163 1630 3916 4057 163
Size: 1467 2286 141 3671
Fragments arranged by size:
3671 2286 1467 141
BglII A'GATC_T
Cuts at: 5080 7117 5080
Size: 2037 5528
BlpI GC'TnA_GC
Cuts at: 4225 4677 4225
Size: 452 7113
Bme1580I G_kGCm'C
Cuts at: 238 884 1691 2168 3623 4178 4377 5190 5283
Size: 646 807 477 1455 555 199 813 93
Cuts at: 5283 6339 238
Size: 1056 1464
Fragments arranged by size:
1464 1455 1056 813 807 646 555 477
199 93
BmrI ACTGGGnnnn_n'
Cuts at: 878 941 3141 3552 3907 4777 4858 5193 7337
Size: 63 2200 411 355 870 81 335 2144
Cuts at: 7337 878
Size: 1106
Fragments arranged by size:
2200 2144 1106 870 411 355 335 81
63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3897 5932 3897
Size: 2035 5530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1360 4494 1360
Size: 3134 4431
Bpu10I CC'TnA_GC
Cuts at: 1052 2104 1052
Size: 1052 6513
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 4741 5620 6112 6410 751
Size: 3990 879 492 298 1906
Fragments arranged by size:
3990 1906 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 325 2118 3641 4404 7269 325
Size: 1793 1523 763 2865 621
Fragments arranged by size:
2865 1793 1523 763 621
BsaAI yAC'GTr
Cuts at: 872 3552 4918 872
Size: 2680 1366 3519
Fragments arranged by size:
3519 2680 1366
BsaBI GATnn'nnATC
Cuts at: 6 1319 5079 7116 6
Size: 1313 3760 2037 455
Fragments arranged by size:
3760 2037 1313 455
BsaHI Gr'CG_yC
Cuts at: 3925 4348 4996 5244 5946 7442 3925
Size: 423 648 248 702 1496 4048
Fragments arranged by size:
4048 1496 702 648 423 248
BsaWI w'CCGG_w
Cuts at: 380 3731 3827 3989 4754 5275 5953 6227 6374
Size: 3351 96 162 765 521 678 274 147
Cuts at: 6374 6785 380
Size: 411 1160
Fragments arranged by size:
3351 1160 765 678 521 411 274 162
147 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 277 307 478 508 1207 1237 1595 1625 2734
Size: 30 171 30 699 30 358 30 1109
Cuts at: 2734 2764 2776 2806 3718 3748 3766 3796 277
Size: 30 12 30 912 30 18 30 4046
Fragments arranged by size:
4046 1109 912 699 358 171 30 30
30 30 30 30 30 30 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 486 721 1357 1465 2755 2986 3183 3629 486
Size: 235 636 108 1290 231 197 446 4422
Fragments arranged by size:
4422 1290 636 446 235 231 197 108
BseYI C'CCAG_C
Cuts at: 205 695 4990 5748 6325 6821 205
Size: 490 4295 758 577 496 949
Fragments arranged by size:
4295 949 758 577 496 490
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1987 3674 3757 1987
Size: 1687 83 5795
Fragments arranged by size:
5795 1687 83
BsiEI CG_ry'CG
Cuts at: 104 1666 2738 3249 3445 5107 5153 6361 104
Size: 1562 1072 511 196 1662 46 1208 1308
Fragments arranged by size:
1662 1562 1308 1208 1072 511 196 46
BsiHKAI G_wGCw'C
Cuts at: 186 1040 2918 3551 4150 4579 5357 5547 6339
Size: 854 1878 633 599 429 778 190 792
Cuts at: 6339 186
Size: 1412
Fragments arranged by size:
1878 1412 854 792 778 633 599 429
190
BsmI GAATG_Cn'
Cuts at: 2934 2934
Size: 7565
BsmBI CGTCTCn'nnnn_
Cuts at: 1818 1818
Size: 7565
Bsp1286I G_dGCh'C
Cuts at: 186 202 238 496 884 938 1040 1058 1642
Size: 16 36 258 388 54 102 18 584
Cuts at: 1642 1691 2168 2918 3116 3551 3623 3961 4111
Size: 49 477 750 198 435 72 338 150
Cuts at: 4111 4150 4178 4377 4579 5190 5283 5357 5547
Size: 39 28 199 202 611 93 74 190
Cuts at: 5547 5609 6339 186
Size: 62 730 1412
Fragments arranged by size:
1412 750 730 611 584 477 435 388
338 258 202 199 198 190 150 102
93 74 72 62 54 49 39 36
28 18 16
BspEI T'CCGG_A
Cuts at: 6785 6785
Size: 7565
BspHI T'CATG_A
Cuts at: 1613 2066 2531 6741 1613
Size: 453 465 4210 2437
Fragments arranged by size:
4210 2437 465 453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1399 3032 3696 4043 4615 4829 5131 5512 1399
Size: 1633 664 347 572 214 302 381 3452
Fragments arranged by size:
3452 1633 664 572 381 347 302 214
BsrBI CCG'CTC
Cuts at: 50 121 3278 3452 3579 5857 5911 7395 7560
Size: 71 3157 174 127 2278 54 1484 165
Cuts at: 7560 50
Size: 55
Fragments arranged by size:
3157 2278 1484 174 165 127 71 55
54
BsrDI GCAATG_nn'
Cuts at: 215 1737 2257 2396 2649 3339 4521 5477 215
Size: 1522 520 139 253 690 1182 956 2303
Fragments arranged by size:
2303 1522 1182 956 690 520 253 139
BsrFI r'CCGG_y
Cuts at: 3074 3731 3903 4004 4392 5563 5744 5953 3074
Size: 657 172 101 388 1171 181 209 4686
Fragments arranged by size:
4686 1171 657 388 209 181 172 101
BssSI C'ACGA_G
Cuts at: 229 268 1081 5836 6194 229
Size: 39 813 4755 358 1600
Fragments arranged by size:
4755 1600 813 358 39
BstAPI GCAn_nnn'nTGC
Cuts at: 3749 4942 3749
Size: 1193 6372
BstBI TT'CG_AA
Cuts at: 5926 5926
Size: 7565
BstEII G'GTnAC_C
Cuts at: 1548 2352 1548
Size: 804 6761
BstXI CCAn_nnnn'nTGG
Cuts at: 891 1998 2098 2829 3088 891
Size: 1107 100 731 259 5368
Fragments arranged by size:
5368 1107 731 259 100
BstYI r'GATC_y
Cuts at: 1 386 437 662 752 4590 5057 5080 5415
Size: 385 51 225 90 3838 467 23 335
Cuts at: 5415 5661 6673 6759 6771 7117 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
3838 1012 467 449 385 346 335 246
225 90 86 51 23 12
BtgI C'CryG_G
Cuts at: 296 486 1820 2591 2724 4302 4334 296
Size: 190 1334 771 133 1578 32 3527
Fragments arranged by size:
3527 1578 1334 771 190 133 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 414 5515 5696 5859 414
Size: 5101 181 163 2120
Fragments arranged by size:
5101 2120 181 163
BtsI GCAGTG_nn'
Cuts at: 1010 1640 2045 2315 2370 2768 3099 4178 4808
Size: 630 405 270 55 398 331 1079 630
Cuts at: 4808 1010
Size: 3767
Fragments arranged by size:
3767 1079 630 630 405 398 331 270
55
ClaI AT'CG_AT
Cuts at: 3107 3107
Size: 7565
DraI TTT'AAA
Cuts at: 4564 4564
Size: 7565
DraIII CAC_nnn'GTG
Cuts at: 3672 3672
Size: 7565
DrdI GACnn_nn'nnGTC
Cuts at: 5271 6129 5271
Size: 858 6707
EaeI y'GGCC_r
Cuts at: 155 164 251 836 2036 2588 2819 3258 3392
Size: 9 87 585 1200 552 231 439 134
Cuts at: 3392 5150 5324 5715 5742 7087 155
Size: 1758 174 391 27 1345 633
Fragments arranged by size:
1758 1345 1200 633 585 552 439 391
231 174 134 87 27 9
EagI C'GGCC_G
Cuts at: 5150 5150
Size: 7565
EarI CTCTTCn'nnn_
Cuts at: 190 1081 1298 1612 2986 5588 5798 190
Size: 891 217 314 1374 2602 210 1957
Fragments arranged by size:
2602 1957 1374 891 314 217 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5773 6083 6229 7375 5773
Size: 310 146 1146 5963
Fragments arranged by size:
5963 1146 310 146
EcoICRI GAG'CTC
Cuts at: 184 4577 184
Size: 4393 3172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1143 1322 1360 1830 2967 3052 3394 3459 4308
Size: 179 38 470 1137 85 342 65 849
Cuts at: 4308 4494 5390 5822 6569 6837 1143
Size: 186 896 432 747 268 1871
Fragments arranged by size:
1871 1137 896 849 747 470 432 342
268 186 179 85 65 38
EcoNI CCTnn'n_nnAGG
Cuts at: 1342 1395 4039 1342
Size: 53 2644 4868
Fragments arranged by size:
4868 2644 53
EcoO109I rG'GnC_Cy
Cuts at: 711 1622 3619 3620 4374 4704 711
Size: 911 1997 1 754 330 3572
Fragments arranged by size:
3572 1997 911 754 330 1
EcoRI G'AATT_C
Cuts at: 4569 4569
Size: 7565
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3489 5584 3489
Size: 2095 5470
FspI TGC'GCA
Cuts at: 2815 5346 2815
Size: 2531 5034
HaeII r_GCGC'y
Cuts at: 1775 2430 2748 2870 3471 4999 5247 6269 7248
Size: 655 318 122 601 1528 248 1022 979
Cuts at: 7248 1775
Size: 2092
Fragments arranged by size:
2092 1528 1022 979 655 601 318 248
122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 358 390 511 543 744 776 1015 1047 1595
Size: 32 121 32 201 32 239 32 548
Cuts at: 1595 1627 1648 1680 2734 2766 2932 2964 3718
Size: 32 21 32 1054 32 166 32 754
Cuts at: 3718 3750 5407 5437 5439 5469 358
Size: 32 1657 30 2 30 2454
Fragments arranged by size:
2454 1657 1054 754 548 239 201 166
121 32 32 32 32 32 32 32
32 32 30 30 21 2
HincII GTy'rAC
Cuts at: 1677 4604 1677
Size: 2927 4638
HindIII A'AGCT_T
Cuts at: 566 566
Size: 7565
KasI G'GCGC_C
Cuts at: 4995 5243 4995
Size: 248 7317
KpnI G_GTAC'C
Cuts at: 304 3688 4585 304
Size: 3384 897 3284
Fragments arranged by size:
3384 3284 897
MfeI C'AATT_G
Cuts at: 554 554
Size: 7565
MluI A'CGCG_T
Cuts at: 6998 6998
Size: 7565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 266 2670 2819 2907 4487 6236 6420 266
Size: 2404 149 88 1580 1749 184 1411
Fragments arranged by size:
2404 1749 1580 1411 184 149 88
MscI TGG'CCA
Cuts at: 253 838 2038 3260 5326 253
Size: 585 1200 1222 2066 2492
Fragments arranged by size:
2492 2066 1222 1200 585
MslI CAynn'nnrTG
Cuts at: 229 1633 3790 4027 4526 5554 5681 229
Size: 1404 2157 237 499 1028 127 2113
Fragments arranged by size:
2157 2113 1404 1028 499 237 127
MspA1I CmG'CkG
Cuts at: 2544 2717 3030 3611 3680 3758 4138 4676 4990
Size: 173 313 581 69 78 380 538 314
Cuts at: 4990 5350 6363 6608 2544
Size: 360 1013 245 3501
Fragments arranged by size:
3501 1013 581 538 380 360 314 313
245 173 78 69
NaeI GCC'GGC
Cuts at: 3905 4006 5746 3905
Size: 101 1740 5724
Fragments arranged by size:
5724 1740 101
NarI GG'CG_CC
Cuts at: 4996 5244 4996
Size: 248 7317
NcoI C'CATG_G
Cuts at: 2591 2724 4302 2591
Size: 133 1578 5854
Fragments arranged by size:
5854 1578 133
NdeI CA'TA_TG
Cuts at: 951 951
Size: 7565
NgoMIV G'CCGG_C
Cuts at: 3903 4004 5744 3903
Size: 101 1740 5724
Fragments arranged by size:
5724 1740 101
NsiI A_TGCA'T
Cuts at: 2216 2259 2216
Size: 43 7522
NspI r_CATG'y
Cuts at: 729 1029 2218 2580 4125 4224 4525 4618 5118
Size: 300 1189 362 1545 99 301 93 500
Cuts at: 5118 6025 729
Size: 907 2269
Fragments arranged by size:
2269 1545 1189 907 500 362 301 300
99 93
PasI CC'CwG_GG
Cuts at: 4493 4493
Size: 7565
PciI A'CATG_T
Cuts at: 725 2576 4121 6021 725
Size: 1851 1545 1900 2269
Fragments arranged by size:
2269 1900 1851 1545
PflMI CCAn_nnn'nTGG
Cuts at: 143 396 2730 143
Size: 253 2334 4978
Fragments arranged by size:
4978 2334 253
PmeI GTTT'AAAC
Cuts at: 4564 4564
Size: 7565
PmlI CAC'GTG
Cuts at: 872 872
Size: 7565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2998 3030 6704 6736 2998
Size: 32 3674 32 3827
Fragments arranged by size:
3827 3674 32 32
PpuMI rG'GwC_Cy
Cuts at: 711 711
Size: 7565
PshAI GACnn'nnGTC
Cuts at: 2889 2889
Size: 7565
PsiI TTA'TAA
Cuts at: 1862 1862
Size: 7565
PspOMI G'GGCC_C
Cuts at: 3619 4373 3619
Size: 754 6811
PstI C_TGCA'G
Cuts at: 1262 2453 3029 3890 4040 4612 5297 1262
Size: 1191 576 861 150 572 685 3530
Fragments arranged by size:
3530 1191 861 685 576 572 150
PvuI CG_AT'CG
Cuts at: 104 1666 2738 104
Size: 1562 1072 4931
Fragments arranged by size:
4931 1562 1072
PvuII CAG'CTG
Cuts at: 2544 2717 3030 3758 4138 4990 5350 2544
Size: 173 313 728 380 852 360 4759
Fragments arranged by size:
4759 852 728 380 360 313 173
RsrII CG'GwC_CG
Cuts at: 3734 5760 3734
Size: 2026 5539
SacI G_AGCT'C
Cuts at: 186 4579 186
Size: 4393 3172
SalI G'TCGA_C
Cuts at: 1675 4602 1675
Size: 2927 4638
SapI GCTCTTCn'nnn_
Cuts at: 190 1298 5588 5798 190
Size: 1108 4290 210 1957
Fragments arranged by size:
4290 1957 1108 210
SbfI CC_TGCA'GG
Cuts at: 3890 4612 3890
Size: 722 6843
ScaI AGT'ACT
Cuts at: 1270 1843 7082 1270
Size: 573 5239 1753
Fragments arranged by size:
5239 1753 573
SexAI A'CCwGG_T
Cuts at: 1544 2090 1544
Size: 546 7019
SfcI C'TryA_G
Cuts at: 33 1258 2449 2712 3025 3252 3886 4036 4608
Size: 1225 1191 263 313 227 634 150 572
Cuts at: 4608 5293 6286 6477 33
Size: 685 993 191 1121
Fragments arranged by size:
1225 1191 1121 993 685 634 572 313
263 227 191 150
SfiI GGCCn_nnn'nGGCC
Cuts at: 163 163
Size: 7565
SfoI GGC'GCC
Cuts at: 4997 5245 4997
Size: 248 7317
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7565
SmaI CCC'GGG
Cuts at: 307 4587 307
Size: 4280 3285
SmlI C'TyrA_G
Cuts at: 766 1501 4720 5635 6127 6389 766
Size: 735 3219 915 492 262 1942
Fragments arranged by size:
3219 1942 915 735 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7565
SphI G_CATG'C
Cuts at: 2218 4618 5118 2218
Size: 2400 500 4665
Fragments arranged by size:
4665 2400 500
SspI AAT'ATT
Cuts at: 2190 2190
Size: 7565
StyI C'CwwG_G
Cuts at: 806 2591 2724 3694 3971 4302 4699 5053 806
Size: 1785 133 970 277 331 397 354 3318
Fragments arranged by size:
3318 1785 970 397 354 331 277 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 349 1093 1773 2408 2650 3160 3577 5148 5281
Size: 744 680 635 242 510 417 1571 133
Cuts at: 5281 5740 5949 349
Size: 459 209 1965
Fragments arranged by size:
1965 1571 744 680 635 510 459 417
242 209 133
TatI w'GTAC_w
Cuts at: 1268 1841 7080 1268
Size: 573 5239 1753
Fragments arranged by size:
5239 1753 573
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 277 309 478 510 1207 1239 2132 2164 3766
Size: 32 169 32 697 32 893 32 1602
Cuts at: 3766 3798 4276 4308 7082 7114 277
Size: 32 478 32 2774 32 728
Fragments arranged by size:
2774 1602 893 728 697 478 169 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3604 4249 5362 3604
Size: 645 1113 5807
Fragments arranged by size:
5807 1113 645
XbaI T'CTAG_A
Cuts at: 4596 4596
Size: 7565
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 144 2413 4392 5060 144
Size: 2269 1979 668 2649
Fragments arranged by size:
2649 2269 1979 668
XmaI C'CCGG_G
Cuts at: 305 4585 305
Size: 4280 3285
XmnI GAAnn'nnTTC
Cuts at: 2980 2980
Size: 7565
ZraI GAC'GTC
Cuts at: 3926 3926
Size: 7565
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AlfI AloI AscI AvrII BaeI BbvCI BmgBI
BmtI BsiWI BsrGI BssHII BstZ17I Bsu36I CspCI EcoRV
FseI FspAI HpaI NheI NotI NruI PacI PfoI
PspXI PsrI SacII SanDI SgrAI SnaBI SrfI StuI
SwaI XhoI