Restriction map for
pF1KE2677
(Circular) (Six-base) MAPSORT of: pF1KE2677.seq Check: 5608 from: 1 to: 8573
pF1KE2677 8573 bp
With 182 enzymes: SgfI *
March 1, 2017 16:47 ..
AccI GT'mk_AC
Cuts at: 670 5611 670
Size: 4941 3632
Acc65I G'GTAC_C
Cuts at: 5589 5589
Size: 8573
AclI AA'CG_TT
Cuts at: 8413 8413
Size: 8573
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 1754 2126 2608 2702 3715 3925 6398 6830
Size: 1587 372 482 94 1013 210 2473 432
Cuts at: 6830 7577 7845 167
Size: 747 268 895
Fragments arranged by size:
2473 1587 1013 895 747 482 432 372
268 210 94
AfeI AGC'GCT
Cuts at: 3551 3551
Size: 8573
AflIII A'CryG_T
Cuts at: 2949 5925 7029 8006 2949
Size: 2976 1104 977 3516
Fragments arranged by size:
3516 2976 1104 977
AgeI A'CCGG_T
Cuts at: 6961 6961
Size: 8573
AleI CACnn'nnGTG
Cuts at: 1118 4414 5041 1118
Size: 3296 627 4650
Fragments arranged by size:
4650 3296 627
AlwNI CAG_nnn'CTG
Cuts at: 3477 3927 4837 5878 7445 3477
Size: 450 910 1041 1567 4605
Fragments arranged by size:
4605 1567 1041 910 450
ApaLI G'TGCA_C
Cuts at: 7343 7343
Size: 8573
ApoI r'AATT_y
Cuts at: 192 226 442 487 910 938 985 1454 1563
Size: 34 216 45 423 28 47 469 109
Cuts at: 1563 1900 1907 2452 2883 2912 3236 3258 3705
Size: 337 7 545 431 29 324 22 447
Cuts at: 3705 3715 4877 5149 5172 5302 5459 5577 7926
Size: 10 1162 272 23 130 157 118 2349
Cuts at: 7926 8182 192
Size: 256 583
Fragments arranged by size:
2349 1162 583 545 469 447 431 423
337 324 272 256 216 157 130 118
109 47 45 34 29 28 23 22
10 7
AseI AT'TA_AT
Cuts at: 20 2299 3058 4067 20
Size: 2279 759 1009 4526
Fragments arranged by size:
4526 2279 1009 759
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8573
AvaI C'yCGr_G
Cuts at: 2060 5593 2060
Size: 3533 5040
BamHI G'GATC_C
Cuts at: 5598 5598
Size: 8573
BanI G'GyrC_C
Cuts at: 5589 6003 6251 6286 5589
Size: 414 248 35 7876
Fragments arranged by size:
7876 414 248 35
BanII G_rGCy'C
Cuts at: 1950 5377 5587 6617 1950
Size: 3427 210 1030 3906
Fragments arranged by size:
3906 3427 1030 210
BbeI G_GCGC'C
Cuts at: 6007 6255 6007
Size: 248 8325
BbsI GAAGACnn'nnnn_
Cuts at: 286 646 1279 1706 1856 2690 2738 4238 286
Size: 360 633 427 150 834 48 1500 4621
Fragments arranged by size:
4621 1500 834 633 427 360 150 48
BbvCI CC'TCA_GC
Cuts at: 4571 4571
Size: 8573
BciVI GTATCCnnnnn_n'
Cuts at: 6471 7232 8573 6471
Size: 761 1341 6471
Fragments arranged by size:
6471 1341 761
BclI T'GATC_A
Cuts at: 2427 4944 5523 6093 2427
Size: 2517 579 570 4907
Fragments arranged by size:
4907 2517 579 570
BfrBI ATG'CAT
Cuts at: 395 395
Size: 8573
BglII A'GATC_T
Cuts at: 1047 3936 4203 6088 8125 1047
Size: 2889 267 1885 2037 1495
Fragments arranged by size:
2889 2037 1885 1495 267
BlpI GC'TnA_GC
Cuts at: 5685 5685
Size: 8573
Bme1580I G_kGCm'C
Cuts at: 6198 6291 7347 6198
Size: 93 1056 7424
Fragments arranged by size:
7424 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1316 5785 5866 6201 8345 1316
Size: 4469 81 335 2144 1544
Fragments arranged by size:
4469 2144 1544 335 81
BmtI G_CTAG'C
Cuts at: 752 752
Size: 8573
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3915 6940 3915
Size: 3025 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 4096 268
Size: 3828 4745
Bpu10I CC'TnA_GC
Cuts at: 423 4571 423
Size: 4148 4425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 1556 1589 2383 3760 4123 4312 4505 5749
Size: 1204 33 794 1377 363 189 193 1244
Cuts at: 5749 6628 7120 7418 352
Size: 879 492 298 1507
Fragments arranged by size:
1507 1377 1244 1204 879 794 492 363
298 193 189 33
BsaI GGTCTCn'nnnn_
Cuts at: 8277 8277
Size: 8573
BsaAI yAC'GTr
Cuts at: 3085 5926 3085
Size: 2841 5732
BsaBI GATnn'nnATC
Cuts at: 6 4513 6087 8124 6
Size: 4507 1574 2037 455
Fragments arranged by size:
4507 2037 1574 455
BsaHI Gr'CG_yC
Cuts at: 6004 6252 6954 8450 6004
Size: 248 702 1496 6127
Fragments arranged by size:
6127 1496 702 248
BsaWI w'CCGG_w
Cuts at: 5762 6283 6961 7235 7382 7793 5762
Size: 521 678 274 147 411 6542
Fragments arranged by size:
6542 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1732 1834 3172 5107 1732
Size: 102 1338 1935 5198
Fragments arranged by size:
5198 1935 1338 102
BseYI C'CCAG_C
Cuts at: 468 4625 5998 6756 7333 7829 468
Size: 4157 1373 758 577 496 1212
Fragments arranged by size:
4157 1373 1212 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2974 2974
Size: 8573
BsiEI CG_ry'CG
Cuts at: 104 2713 6115 6161 7369 104
Size: 2609 3402 46 1208 1308
Fragments arranged by size:
3402 2609 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 5377 5587 6365 6555 7347 5377
Size: 210 778 190 792 6603
Fragments arranged by size:
6603 792 778 210 190
BsmI GAATG_Cn'
Cuts at: 617 5130 617
Size: 4513 4060
Bsp1286I G_dGCh'C
Cuts at: 1950 5377 5587 6198 6291 6365 6555 6617 7347
Size: 3427 210 611 93 74 190 62 730
Cuts at: 7347 1950
Size: 3176
Fragments arranged by size:
3427 3176 730 611 210 190 93 74
62
BspEI T'CCGG_A
Cuts at: 7793 7793
Size: 8573
BspHI T'CATG_A
Cuts at: 222 2990 7749 222
Size: 2768 4759 1046
Fragments arranged by size:
4759 2768 1046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 5623 5837 6139 6520 5623
Size: 214 302 381 7676
Fragments arranged by size:
7676 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6865 6919 8403 8568 50
Size: 6815 54 1484 165 55
Fragments arranged by size:
6815 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 760 1529 2502 2717 4362 6485 760
Size: 769 973 215 1645 2123 2848
Fragments arranged by size:
2848 2123 1645 973 769 215
BsrFI r'CCGG_y
Cuts at: 6571 6752 6961 6571
Size: 181 209 8183
Fragments arranged by size:
8183 209 181
BsrGI T'GTAC_A
Cuts at: 4728 4728
Size: 8573
BssSI C'ACGA_G
Cuts at: 1211 1816 6844 7202 1211
Size: 605 5028 358 2582
Fragments arranged by size:
5028 2582 605 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3910 5950 3910
Size: 2040 6533
BstBI TT'CG_AA
Cuts at: 266 297 5550 6934 266
Size: 31 5253 1384 1905
Fragments arranged by size:
5253 1905 1384 31
BstXI CCAn_nnnn'nTGG
Cuts at: 4332 4332
Size: 8573
BstYI r'GATC_y
Cuts at: 1 697 1047 3138 3936 4203 4833 4903 5598
Size: 696 350 2091 798 267 630 70 695
Cuts at: 5598 6065 6088 6423 6669 7681 7767 7779 8125
Size: 467 23 335 246 1012 86 12 346
Cuts at: 8125 1
Size: 449
Fragments arranged by size:
2091 1012 798 696 695 630 467 449
350 346 335 267 246 86 70 23
12
BstZ17I GTA'TAC
Cuts at: 671 671
Size: 8573
Bsu36I CC'TnA_GG
Cuts at: 2567 2567
Size: 8573
BtgI C'CryG_G
Cuts at: 107 107
Size: 8573
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6523 6704 6867 6523
Size: 181 163 8229
Fragments arranged by size:
8229 181 163
BtsI GCAGTG_nn'
Cuts at: 5021 5816 5021
Size: 795 7778
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2079 2114 2079
Size: 35 8538
DraI TTT'AAA
Cuts at: 1097 1562 1905 2490 3761 5572 1097
Size: 465 343 585 1271 1811 4098
Fragments arranged by size:
4098 1811 1271 585 465 343
DraIII CAC_nnn'GTG
Cuts at: 3571 3571
Size: 8573
DrdI GACnn_nn'nnGTC
Cuts at: 542 6279 7137 542
Size: 5737 858 1978
Fragments arranged by size:
5737 1978 858
EaeI y'GGCC_r
Cuts at: 4322 5270 6158 6332 6723 6750 8095 4322
Size: 948 888 174 391 27 1345 4800
Fragments arranged by size:
4800 1345 948 888 391 174 27
EagI C'GGCC_G
Cuts at: 6158 6158
Size: 8573
EarI CTCTTCn'nnn_
Cuts at: 2156 2219 2309 3324 5065 5071 5382 6596 6806
Size: 63 90 1015 1741 6 311 1214 210
Cuts at: 6806 2156
Size: 3923
Fragments arranged by size:
3923 1741 1214 1015 311 210 90 63
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1515 6781 7091 7237 8383 1515
Size: 5266 310 146 1146 1705
Fragments arranged by size:
5266 1705 1146 310 146
EcoICRI GAG'CTC
Cuts at: 5375 5585 5375
Size: 210 8363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 268 1754 2126 2608 2702 3715 3925 4096
Size: 101 1486 372 482 94 1013 210 171
Cuts at: 4096 6398 6830 7577 7845 167
Size: 2302 432 747 268 895
Fragments arranged by size:
2302 1486 1013 895 747 482 432 372
268 210 171 101 94
EcoO109I rG'GnC_Cy
Cuts at: 5712 5712
Size: 8573
EcoRI G'AATT_C
Cuts at: 487 3236 5577 487
Size: 2749 2341 3483
Fragments arranged by size:
3483 2749 2341
EcoRV GAT'ATC
Cuts at: 4718 4806 4718
Size: 88 8485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 507 3481 3745 3955 6592 507
Size: 2974 264 210 2637 2488
Fragments arranged by size:
2974 2637 2488 264 210
FspI TGC'GCA
Cuts at: 6354 6354
Size: 8573
HaeII r_GCGC'y
Cuts at: 3553 6007 6255 7277 8256 3553
Size: 2454 248 1022 979 3870
Fragments arranged by size:
3870 2454 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2285 2317 2563 2595 4888 4895 4920 4927 6415
Size: 32 246 32 2293 7 25 7 1488
Cuts at: 6415 6445 6447 6477 2285
Size: 30 2 30 4381
Fragments arranged by size:
4381 2293 1488 246 32 32 30 30
25 7 7 2
HincII GTy'rAC
Cuts at: 3803 4934 5612 3803
Size: 1131 678 6764
Fragments arranged by size:
6764 1131 678
HindIII A'AGCT_T
Cuts at: 208 208
Size: 8573
HpaI GTT'AAC
Cuts at: 3803 3803
Size: 8573
KasI G'GCGC_C
Cuts at: 6003 6251 6003
Size: 248 8325
KpnI G_GTAC'C
Cuts at: 5593 5593
Size: 8573
MfeI C'AATT_G
Cuts at: 170 2936 170
Size: 2766 5807
MluI A'CGCG_T
Cuts at: 8006 8006
Size: 8573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1817 2931 7244 7428 1817
Size: 1114 4313 184 2962
Fragments arranged by size:
4313 2962 1114 184
MscI TGG'CCA
Cuts at: 4324 5272 6334 4324
Size: 948 1062 6563
Fragments arranged by size:
6563 1062 948
MslI CAynn'nnrTG
Cuts at: 1118 2120 2490 4240 4414 5041 6562 6689 1118
Size: 1002 370 1750 174 627 1521 127 3002
Fragments arranged by size:
3002 1750 1521 1002 627 370 174 127
MspA1I CmG'CkG
Cuts at: 3477 4629 5684 5998 6358 7371 7616 3477
Size: 1152 1055 314 360 1013 245 4434
Fragments arranged by size:
4434 1152 1055 1013 360 314 245
NaeI GCC'GGC
Cuts at: 6754 6754
Size: 8573
NarI GG'CG_CC
Cuts at: 6004 6252 6004
Size: 248 8325
NcoI C'CATG_G
Cuts at: 107 107
Size: 8573
NdeI CA'TA_TG
Cuts at: 2474 2474
Size: 8573
NgoMIV G'CCGG_C
Cuts at: 6752 6752
Size: 8573
NheI G'CTAG_C
Cuts at: 748 748
Size: 8573
NsiI A_TGCA'T
Cuts at: 397 397
Size: 8573
NspI r_CATG'y
Cuts at: 1393 2953 3532 4199 4239 5626 6126 7033 1393
Size: 1560 579 667 40 1387 500 907 2933
Fragments arranged by size:
2933 1560 1387 907 667 579 500 40
PciI A'CATG_T
Cuts at: 2949 7029 2949
Size: 4080 4493
PflMI CCAn_nnn'nTGG
Cuts at: 1670 1670
Size: 8573
PfoI T'CCnGG_A
Cuts at: 1400 1400
Size: 8573
PmeI GTTT'AAAC
Cuts at: 5572 5572
Size: 8573
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7712 7744 7712
Size: 32 8541
PsiI TTA'TAA
Cuts at: 1042 1042
Size: 8573
PstI C_TGCA'G
Cuts at: 5620 6305 5620
Size: 685 7888
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8573
PvuII CAG'CTG
Cuts at: 3477 4629 5998 6358 3477
Size: 1152 1369 360 5692
Fragments arranged by size:
5692 1369 1152 360
RsrII CG'GwC_CG
Cuts at: 6768 6768
Size: 8573
SacI G_AGCT'C
Cuts at: 5377 5587 5377
Size: 210 8363
SalI G'TCGA_C
Cuts at: 5610 5610
Size: 8573
SapI GCTCTTCn'nnn_
Cuts at: 5382 6596 6806 5382
Size: 1214 210 7149
Fragments arranged by size:
7149 1214 210
SbfI CC_TGCA'GG
Cuts at: 5620 5620
Size: 8573
ScaI AGT'ACT
Cuts at: 8090 8090
Size: 8573
SfcI C'TryA_G
Cuts at: 33 1086 4907 5296 5616 6301 7294 7485 33
Size: 1053 3821 389 320 685 993 191 1121
Fragments arranged by size:
3821 1121 1053 993 685 389 320 191
SfoI GGC'GCC
Cuts at: 6005 6253 6005
Size: 248 8325
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8573
SmaI CCC'GGG
Cuts at: 5595 5595
Size: 8573
SmlI C'TyrA_G
Cuts at: 331 1571 1604 2060 2362 3775 4138 4291 4520
Size: 1240 33 456 302 1413 363 153 229
Cuts at: 4520 5728 6643 7135 7397 331
Size: 1208 915 492 262 1507
Fragments arranged by size:
1507 1413 1240 1208 915 492 456 363
302 262 229 153 33
SnaBI TAC'GTA
Cuts at: 3085 3085
Size: 8573
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8573
SphI G_CATG'C
Cuts at: 4199 5626 6126 4199
Size: 1427 500 6646
Fragments arranged by size:
6646 1427 500
SspI AAT'ATT
Cuts at: 612 1184 1643 612
Size: 572 459 7542
Fragments arranged by size:
7542 572 459
StuI AGG'CCT
Cuts at: 180 3316 180
Size: 3136 5437
StyI C'CwwG_G
Cuts at: 107 399 455 1895 5060 5707 6061 107
Size: 292 56 1440 3165 647 354 2619
Fragments arranged by size:
3165 2619 1440 647 354 292 56
SwaI ATTT'AAAT
Cuts at: 2490 3761 2490
Size: 1271 7302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2727 5148 6156 6289 6748 6957 2727
Size: 2421 1008 133 459 209 4343
Fragments arranged by size:
4343 2421 1008 459 209 133
TatI w'GTAC_w
Cuts at: 4011 4728 8088 4011
Size: 717 3360 4496
Fragments arranged by size:
4496 3360 717
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4944 4976 8090 8122 4944
Size: 32 3114 32 5395
Fragments arranged by size:
5395 3114 32 32
Tth111I GACn'n_nGTC
Cuts at: 539 6370 539
Size: 5831 2742
XbaI T'CTAG_A
Cuts at: 1683 5604 1683
Size: 3921 4652
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 891 6068 891
Size: 5177 3396
XhoI C'TCGA_G
Cuts at: 2060 2060
Size: 8573
XmaI C'CCGG_G
Cuts at: 5593 5593
Size: 8573
XmnI GAAnn'nnTTC
Cuts at: 487 491 3728 5175 487
Size: 4 3237 1447 3885
Fragments arranged by size:
3885 3237 1447 4
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI
NspI PciI PflMI PfoI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BsaXI BsiWI BsmBI
BssHII BstEII ClaI EcoNI FseI FspAI NotI NruI
PacI PasI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI ZraI