Restriction map for
pF1KE2665
(Circular) (Six-base) MAPSORT of: pF1KE2665.seq Check: 855 from: 1 to: 4232
pF1KE2665 4232 bp
With 182 enzymes: SgfI *
January 12, 2017 11:32 ..
AccI GT'mk_AC
Cuts at: 464 975 1270 464
Size: 511 295 3426
Fragments arranged by size:
3426 511 295
Acc65I G'GTAC_C
Cuts at: 1248 1248
Size: 4232
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 2057 2489 3236 3504 448
Size: 1609 432 747 268 1176
Fragments arranged by size:
1609 1176 747 432 268
AflIII A'CryG_T
Cuts at: 914 1584 2688 3665 914
Size: 670 1104 977 1481
Fragments arranged by size:
1481 1104 977 670
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AlwNI CAG_nnn'CTG
Cuts at: 357 473 1023 1149 1218 1537 3104 357
Size: 116 550 126 69 319 1567 1485
Fragments arranged by size:
1567 1485 550 319 126 116 69
ApaLI G'TGCA_C
Cuts at: 3002 3002
Size: 4232
ApoI r'AATT_y
Cuts at: 126 360 1236 3585 3841 126
Size: 234 876 2349 256 517
Fragments arranged by size:
2349 876 517 256 234
AseI AT'TA_AT
Cuts at: 20 782 20
Size: 762 3470
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 872 1252 872
Size: 380 3852
BamHI G'GATC_C
Cuts at: 793 1257 793
Size: 464 3768
BanI G'GyrC_C
Cuts at: 1248 1662 1910 1945 1248
Size: 414 248 35 3535
Fragments arranged by size:
3535 414 248 35
BanII G_rGCy'C
Cuts at: 1246 2276 1246
Size: 1030 3202
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3984
BbsI GAAGACnn'nnnn_
Cuts at: 761 761
Size: 4232
BbvCI CC'TCA_GC
Cuts at: 112 1211 112
Size: 1099 3133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4198
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2891 4232 2130
Size: 761 1341 2130
Fragments arranged by size:
2130 1341 761
BclI T'GATC_A
Cuts at: 644 1752 644
Size: 1108 3124
BfrBI ATG'CAT
Cuts at: 155 155
Size: 4232
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 1344 1344
Size: 4232
Bme1580I G_kGCm'C
Cuts at: 169 1857 1950 3006 169
Size: 1688 93 1056 1395
Fragments arranged by size:
1688 1395 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1444 1525 1860 4004 1444
Size: 81 335 2144 1672
Fragments arranged by size:
2144 1672 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 4232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 1240
Size: 4232
Bpu10I CC'TnA_GC
Cuts at: 112 1211 112
Size: 1099 3133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 2287 2779 3077 1408
Size: 879 492 298 2563
Fragments arranged by size:
2563 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3936 3936
Size: 4232
BsaAI yAC'GTr
Cuts at: 1585 1585
Size: 4232
BsaBI GATnn'nnATC
Cuts at: 6 1746 3783 6
Size: 1740 2037 455
Fragments arranged by size:
2037 1740 455
BsaHI Gr'CG_yC
Cuts at: 1663 1911 2613 4109 1663
Size: 248 702 1496 1786
Fragments arranged by size:
1786 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1421 1942 2620 2894 3041 3452 1421
Size: 521 678 274 147 411 2201
Fragments arranged by size:
2201 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 194 860 194
Size: 666 3566
BseYI C'CCAG_C
Cuts at: 1657 2415 2992 3488 1657
Size: 758 577 496 2401
Fragments arranged by size:
2401 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 871 688
Size: 183 4049
BsiEI CG_ry'CG
Cuts at: 104 1774 1820 3028 104
Size: 1670 46 1208 1308
Fragments arranged by size:
1670 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1246 2024 2214 3006 1246
Size: 778 190 792 2472
Fragments arranged by size:
2472 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 169 1246 1857 1950 2024 2214 2276 3006 169
Size: 1077 611 93 74 190 62 730 1395
Fragments arranged by size:
1395 1077 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 3408 3408
Size: 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1031 1282 1496 1798 2179 1031
Size: 251 214 302 381 3084
Fragments arranged by size:
3084 381 302 251 214
BsrBI CCG'CTC
Cuts at: 50 2524 2578 4062 4227 50
Size: 2474 54 1484 165 55
Fragments arranged by size:
2474 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 251 2144 251
Size: 1893 2339
BsrFI r'CCGG_y
Cuts at: 2230 2411 2620 2230
Size: 181 209 3842
Fragments arranged by size:
3842 209 181
BssSI C'ACGA_G
Cuts at: 2503 2861 2503
Size: 358 3874
BstAPI GCAn_nnn'nTGC
Cuts at: 1609 1609
Size: 4232
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstYI r'GATC_y
Cuts at: 1 423 793 1103 1146 1257 1724 1747 2082
Size: 422 370 310 43 111 467 23 335
Cuts at: 2082 2328 3340 3426 3438 3784 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 422 370 346 335 310
246 111 86 43 23 12
BstZ17I GTA'TAC
Cuts at: 465 465
Size: 4232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2182 2363 2526 2182
Size: 181 163 3888
Fragments arranged by size:
3888 181 163
BtsI GCAGTG_nn'
Cuts at: 854 1125 1475 854
Size: 271 350 3611
Fragments arranged by size:
3611 350 271
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DrdI GACnn_nn'nnGTC
Cuts at: 315 1938 2796 315
Size: 1623 858 1751
Fragments arranged by size:
1751 1623 858
EaeI y'GGCC_r
Cuts at: 1817 1991 2382 2409 3754 1817
Size: 174 391 27 1345 2295
Fragments arranged by size:
2295 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1817 1817
Size: 4232
EarI CTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2440 2750 2896 4042 2440
Size: 310 146 1146 2630
Fragments arranged by size:
2630 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1244 1244
Size: 4232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1240 2057 2489 3236 3504 448
Size: 792 817 432 747 268 1176
Fragments arranged by size:
1176 817 792 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1020 1371 1020
Size: 351 3881
EcoRI G'AATT_C
Cuts at: 360 1236 360
Size: 876 3356
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 4232
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 1666 1914 2936 3915 1666
Size: 248 1022 979 1983
Fragments arranged by size:
1983 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2074 2104 2106 2136 2074
Size: 30 2 30 4170
Fragments arranged by size:
4170 30 30 2
HincII GTy'rAC
Cuts at: 1154 1271 1154
Size: 117 4115
HindIII A'AGCT_T
Cuts at: 1196 1196
Size: 4232
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3984
KpnI G_GTAC'C
Cuts at: 1252 1252
Size: 4232
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 352 612 899 2903 3087 352
Size: 260 287 2004 184 1497
Fragments arranged by size:
2004 1497 287 260 184
MscI TGG'CCA
Cuts at: 1993 1993
Size: 4232
MslI CAynn'nnrTG
Cuts at: 2221 2348 2221
Size: 127 4105
MspA1I CmG'CkG
Cuts at: 1343 1657 2017 3030 3275 1343
Size: 314 360 1013 245 2300
Fragments arranged by size:
2300 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2413 2413
Size: 4232
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3984
NgoMIV G'CCGG_C
Cuts at: 2411 2411
Size: 4232
NsiI A_TGCA'T
Cuts at: 157 157
Size: 4232
NspI r_CATG'y
Cuts at: 918 1285 1785 2692 918
Size: 367 500 907 2458
Fragments arranged by size:
2458 907 500 367
PciI A'CATG_T
Cuts at: 914 2688 914
Size: 1774 2458
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 4200
PpuMI rG'GwC_Cy
Cuts at: 1020 1020
Size: 4232
PstI C_TGCA'G
Cuts at: 246 1279 1964 246
Size: 1033 685 2514
Fragments arranged by size:
2514 1033 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 1657 2017 1657
Size: 360 3872
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4232
SacI G_AGCT'C
Cuts at: 1246 1246
Size: 4232
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 453 3749 453
Size: 3296 936
SfcI C'TryA_G
Cuts at: 33 242 1275 1960 2953 3144 33
Size: 209 1033 685 993 191 1121
Fragments arranged by size:
1121 1033 993 685 209 191
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3984
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 1254 1254
Size: 4232
SmlI C'TyrA_G
Cuts at: 872 1387 2302 2794 3056 872
Size: 515 915 492 262 2048
Fragments arranged by size:
2048 915 515 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 1285 1785 1285
Size: 500 3732
StuI AGG'CCT
Cuts at: 343 396 856 343
Size: 53 460 3719
Fragments arranged by size:
3719 460 53
StyI C'CwwG_G
Cuts at: 174 1366 1720 174
Size: 1192 354 2686
Fragments arranged by size:
2686 1192 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1815 1948 2407 2616 1815
Size: 133 459 209 3431
Fragments arranged by size:
3431 459 209 133
TatI w'GTAC_w
Cuts at: 451 937 3747 451
Size: 486 2810 936
Fragments arranged by size:
2810 936 486
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 4200
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 981 1263 981
Size: 282 3950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1727 1727
Size: 4232
XhoI C'TCGA_G
Cuts at: 872 872
Size: 4232
XmaI C'CCGG_G
Cuts at: 1252 1252
Size: 4232
XmnI GAAnn'nnTTC
Cuts at: 687 687
Size: 4232
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI