Restriction map for
pF1KE2659
(Circular) (Six-base) MAPSORT of: pF1KE2659.seq Check: 6171 from: 1 to: 4583
pF1KE2659 4583 bp
With 182 enzymes: SgfI *
January 20, 2017 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1162 1162
Size: 4583
AatII G_ACGT'C
Cuts at: 185 185
Size: 4583
AccI GT'mk_AC
Cuts at: 1621 1621
Size: 4583
Acc65I G'GTAC_C
Cuts at: 1599 1599
Size: 4583
AclI AA'CG_TT
Cuts at: 4423 4423
Size: 4583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 570 2408 2840 3587 3855 570
Size: 1838 432 747 268 1298
Fragments arranged by size:
1838 1298 747 432 268
AflIII A'CryG_T
Cuts at: 1935 3039 4016 1935
Size: 1104 977 2502
Fragments arranged by size:
2502 1104 977
AgeI A'CCGG_T
Cuts at: 2971 2971
Size: 4583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 399 399
Size: 4583
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1226 1258 1226
Size: 32 4551
AlwNI CAG_nnn'CTG
Cuts at: 1888 3455 1888
Size: 1567 3016
ApaI G_GGCC'C
Cuts at: 421 421
Size: 4583
ApaLI G'TGCA_C
Cuts at: 3353 3353
Size: 4583
ApoI r'AATT_y
Cuts at: 1272 1587 3936 4192 1272
Size: 315 2349 256 1663
Fragments arranged by size:
2349 1663 315 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4583
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
AvaI C'yCGr_G
Cuts at: 404 414 491 1603 404
Size: 10 77 1112 3384
Fragments arranged by size:
3384 1112 77 10
BamHI G'GATC_C
Cuts at: 335 1608 335
Size: 1273 3310
BanI G'GyrC_C
Cuts at: 435 1599 2013 2261 2296 435
Size: 1164 414 248 35 2722
Fragments arranged by size:
2722 1164 414 248 35
BanII G_rGCy'C
Cuts at: 317 421 562 1201 1531 1560 1597 2627 317
Size: 104 141 639 330 29 37 1030 2273
Fragments arranged by size:
2273 1030 639 330 141 104 37 29
BbeI G_GCGC'C
Cuts at: 2017 2265 2017
Size: 248 4335
BbsI GAAGACnn'nnnn_
Cuts at: 1365 1365
Size: 4583
BbvCI CC'TCA_GC
Cuts at: 1035 1324 1035
Size: 289 4294
BciVI GTATCCnnnnn_n'
Cuts at: 2481 3242 4583 2481
Size: 761 1341 2481
Fragments arranged by size:
2481 1341 761
BclI T'GATC_A
Cuts at: 1350 1460 2103 1350
Size: 110 643 3830
Fragments arranged by size:
3830 643 110
BglI GCCn_nnn'nGGC
Cuts at: 312 312
Size: 4583
BglII A'GATC_T
Cuts at: 509 2098 4135 509
Size: 1589 2037 957
Fragments arranged by size:
2037 1589 957
BlpI GC'TnA_GC
Cuts at: 1695 1695
Size: 4583
Bme1580I G_kGCm'C
Cuts at: 421 671 2208 2301 3357 421
Size: 250 1537 93 1056 1647
Fragments arranged by size:
1647 1537 1056 250 93
BmgBI CAC'GTC
Cuts at: 320 485 672 320
Size: 165 187 4231
Fragments arranged by size:
4231 187 165
BmrI ACTGGGnnnn_n'
Cuts at: 1795 1876 2211 4355 1795
Size: 81 335 2144 2023
Fragments arranged by size:
2144 2023 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2950 2950
Size: 4583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1215 1215
Size: 4583
Bpu10I CC'TnA_GC
Cuts at: 1035 1324 1525 1530 1035
Size: 289 201 5 4088
Fragments arranged by size:
4088 289 201 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 487 1759 2638 3130 3428 214
Size: 273 1272 879 492 298 1369
Fragments arranged by size:
1369 1272 879 492 298 273
BsaI GGTCTCn'nnnn_
Cuts at: 4287 4287
Size: 4583
BsaAI yAC'GTr
Cuts at: 617 663 1936 617
Size: 46 1273 3264
Fragments arranged by size:
3264 1273 46
BsaBI GATnn'nnATC
Cuts at: 6 2097 4134 6
Size: 2091 2037 455
Fragments arranged by size:
2091 2037 455
BsaHI Gr'CG_yC
Cuts at: 182 2014 2262 2964 4460 182
Size: 1832 248 702 1496 305
Fragments arranged by size:
1832 1496 702 305 248
BsaWI w'CCGG_w
Cuts at: 177 1772 2293 2971 3245 3392 3803 177
Size: 1595 521 678 274 147 411 957
Fragments arranged by size:
1595 957 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 453 483 988 1018 453
Size: 30 505 30 4018
Fragments arranged by size:
4018 505 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 183 343 409 537 1066 1414 1456 1513 183
Size: 160 66 128 529 348 42 57 3253
Fragments arranged by size:
3253 529 348 160 128 66 57 42
BseYI C'CCAG_C
Cuts at: 127 215 2008 2766 3343 3839 127
Size: 88 1793 758 577 496 871
Fragments arranged by size:
1793 871 758 577 496 88
BsiEI CG_ry'CG
Cuts at: 104 443 530 2125 2171 3379 104
Size: 339 87 1595 46 1208 1308
Fragments arranged by size:
1595 1308 1208 339 87 46
BsiHKAI G_wGCw'C
Cuts at: 490 547 1201 1531 1597 2375 2565 3357 490
Size: 57 654 330 66 778 190 792 1716
Fragments arranged by size:
1716 792 778 654 330 190 66 57
BsmI GAATG_Cn'
Cuts at: 724 1157 1344 724
Size: 433 187 3963
Fragments arranged by size:
3963 433 187
Bsp1286I G_dGCh'C
Cuts at: 317 421 490 547 562 671 1201 1531 1560
Size: 104 69 57 15 109 530 330 29
Cuts at: 1560 1597 2208 2301 2375 2565 2627 3357 317
Size: 37 611 93 74 190 62 730 1543
Fragments arranged by size:
1543 730 611 530 330 190 109 104
93 74 69 62 57 37 29 15
BspEI T'CCGG_A
Cuts at: 3803 3803
Size: 4583
BspHI T'CATG_A
Cuts at: 3759 3759
Size: 4583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1162 1633 1847 2149 2530 1162
Size: 471 214 302 381 3215
Fragments arranged by size:
3215 471 381 302 214
BsrBI CCG'CTC
Cuts at: 50 333 2875 2929 4413 4578 50
Size: 283 2542 54 1484 165 55
Fragments arranged by size:
2542 1484 283 165 55 54
BsrDI GCAATG_nn'
Cuts at: 152 2495 152
Size: 2343 2240
BsrFI r'CCGG_y
Cuts at: 306 438 454 2581 2762 2971 306
Size: 132 16 2127 181 209 1918
Fragments arranged by size:
2127 1918 209 181 132 16
BssSI C'ACGA_G
Cuts at: 549 2854 3212 549
Size: 2305 358 1920
Fragments arranged by size:
2305 1920 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1960 1960
Size: 4583
BstBI TT'CG_AA
Cuts at: 2944 2944
Size: 4583
BstEII G'GTnAC_C
Cuts at: 173 173
Size: 4583
BstXI CCAn_nnnn'nTGG
Cuts at: 1445 1445
Size: 4583
BstYI r'GATC_y
Cuts at: 1 264 291 335 509 1608 2075 2098 2433
Size: 263 27 44 174 1099 467 23 335
Cuts at: 2433 2679 3691 3777 3789 4135 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1099 1012 467 449 346 335 263 246
174 86 44 27 23 12
BtgI C'CryG_G
Cuts at: 107 284 1216 107
Size: 177 932 3474
Fragments arranged by size:
3474 932 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 942 1002 1377 2533 2714 2877 942
Size: 60 375 1156 181 163 2648
Fragments arranged by size:
2648 1156 375 181 163 60
BtsI GCAGTG_nn'
Cuts at: 1092 1349 1826 1092
Size: 257 477 3849
Fragments arranged by size:
3849 477 257
ClaI AT'CG_AT
Cuts at: 1042 1042
Size: 4583
DraI TTT'AAA
Cuts at: 1582 1582
Size: 4583
DrdI GACnn_nn'nnGTC
Cuts at: 2289 3147 2289
Size: 858 3725
EaeI y'GGCC_r
Cuts at: 440 941 2168 2342 2733 2760 4105 440
Size: 501 1227 174 391 27 1345 918
Fragments arranged by size:
1345 1227 918 501 391 174 27
EagI C'GGCC_G
Cuts at: 440 2168 440
Size: 1728 2855
EarI CTCTTCn'nnn_
Cuts at: 1150 1548 2606 2816 1150
Size: 398 1058 210 2917
Fragments arranged by size:
2917 1058 398 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 439 2791 3101 3247 4393 439
Size: 2352 310 146 1146 629
Fragments arranged by size:
2352 1146 629 310 146
EcoICRI GAG'CTC
Cuts at: 1199 1529 1595 1199
Size: 330 66 4187
Fragments arranged by size:
4187 330 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 570 1215 2408 2840 3587 3855 570
Size: 645 1193 432 747 268 1298
Fragments arranged by size:
1298 1193 747 645 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1057 1438 1057
Size: 381 4202
EcoO109I rG'GnC_Cy
Cuts at: 1534 1722 1534
Size: 188 4395
EcoRI G'AATT_C
Cuts at: 1587 1587
Size: 4583
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 528 600 2602 528
Size: 72 2002 2509
Fragments arranged by size:
2509 2002 72
FspI TGC'GCA
Cuts at: 343 2364 343
Size: 2021 2562
HaeII r_GCGC'y
Cuts at: 402 1517 2017 2265 3287 4266 402
Size: 1115 500 248 1022 979 719
Fragments arranged by size:
1115 1022 979 719 500 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 453 485 679 711 1513 1545 2425 2455 2457
Size: 32 194 32 802 32 880 30 2
Cuts at: 2457 2487 453
Size: 30 2549
Fragments arranged by size:
2549 880 802 194 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1622 1622
Size: 4583
HindIII A'AGCT_T
Cuts at: 878 878
Size: 4583
KasI G'GCGC_C
Cuts at: 2013 2261 2013
Size: 248 4335
KpnI G_GTAC'C
Cuts at: 1603 1603
Size: 4583
MluI A'CGCG_T
Cuts at: 4016 4016
Size: 4583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 227 3254 3438 227
Size: 3027 184 1372
Fragments arranged by size:
3027 1372 184
MscI TGG'CCA
Cuts at: 2344 2344
Size: 4583
MslI CAynn'nnrTG
Cuts at: 928 970 1303 1333 2572 2699 928
Size: 42 333 30 1239 127 2812
Fragments arranged by size:
2812 1239 333 127 42 30
MspA1I CmG'CkG
Cuts at: 131 302 476 1694 2008 2368 3381 3626 131
Size: 171 174 1218 314 360 1013 245 1088
Fragments arranged by size:
1218 1088 1013 360 314 245 174 171
NaeI GCC'GGC
Cuts at: 456 2764 456
Size: 2308 2275
NarI GG'CG_CC
Cuts at: 2014 2262 2014
Size: 248 4335
NcoI C'CATG_G
Cuts at: 107 1216 107
Size: 1109 3474
NgoMIV G'CCGG_C
Cuts at: 454 2762 454
Size: 2308 2275
NspI r_CATG'y
Cuts at: 826 1636 2136 3043 826
Size: 810 500 907 2366
Fragments arranged by size:
2366 907 810 500
PasI CC'CwG_GG
Cuts at: 140 371 140
Size: 231 4352
PciI A'CATG_T
Cuts at: 3039 3039
Size: 4583
PflMI CCAn_nnn'nTGG
Cuts at: 632 632
Size: 4583
PmeI GTTT'AAAC
Cuts at: 1582 1582
Size: 4583
PmlI CAC'GTG
Cuts at: 617 617
Size: 4583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 3722
Size: 32 4551
PpuMI rG'GwC_Cy
Cuts at: 1534 1534
Size: 4583
PspOMI G'GGCC_C
Cuts at: 417 417
Size: 4583
PstI C_TGCA'G
Cuts at: 1509 1630 2315 1509
Size: 121 685 3777
Fragments arranged by size:
3777 685 121
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4583
PvuII CAG'CTG
Cuts at: 2008 2368 2008
Size: 360 4223
RsrII CG'GwC_CG
Cuts at: 2778 2778
Size: 4583
SacI G_AGCT'C
Cuts at: 1201 1531 1597 1201
Size: 330 66 4187
Fragments arranged by size:
4187 330 66
SalI G'TCGA_C
Cuts at: 1620 1620
Size: 4583
SapI GCTCTTCn'nnn_
Cuts at: 1548 2606 2816 1548
Size: 1058 210 3315
Fragments arranged by size:
3315 1058 210
SbfI CC_TGCA'GG
Cuts at: 1630 1630
Size: 4583
ScaI AGT'ACT
Cuts at: 235 4100 235
Size: 3865 718
SfcI C'TryA_G
Cuts at: 33 1505 1626 2311 3304 3495 33
Size: 1472 121 685 993 191 1121
Fragments arranged by size:
1472 1121 993 685 191 121
SfoI GGC'GCC
Cuts at: 2015 2263 2015
Size: 248 4335
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
SmaI CCC'GGG
Cuts at: 406 416 493 1605 406
Size: 10 77 1112 3384
Fragments arranged by size:
3384 1112 77 10
SmlI C'TyrA_G
Cuts at: 229 502 1738 2653 3145 3407 229
Size: 273 1236 915 492 262 1405
Fragments arranged by size:
1405 1236 915 492 273 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4583
SphI G_CATG'C
Cuts at: 1636 2136 1636
Size: 500 4083
SspI AAT'ATT
Cuts at: 1263 1263
Size: 4583
StyI C'CwwG_G
Cuts at: 107 770 1216 1717 2071 107
Size: 663 446 501 354 2619
Fragments arranged by size:
2619 663 501 446 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 153 1117 2166 2299 2758 2967 153
Size: 964 1049 133 459 209 1769
Fragments arranged by size:
1769 1049 964 459 209 133
TatI w'GTAC_w
Cuts at: 233 656 815 4098 233
Size: 423 159 3283 718
Fragments arranged by size:
3283 718 423 159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 988 1020 1143 1175 4100 4132 988
Size: 32 123 32 2925 32 1439
Fragments arranged by size:
2925 1439 123 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2380 2380
Size: 4583
XbaI T'CTAG_A
Cuts at: 1614 1614
Size: 4583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 2078 306
Size: 1772 2811
XmaI C'CCGG_G
Cuts at: 404 414 491 1603 404
Size: 10 77 1112 3384
Fragments arranged by size:
3384 1112 77 10
XmnI GAAnn'nnTTC
Cuts at: 511 511
Size: 4583
ZraI GAC'GTC
Cuts at: 183 183
Size: 4583
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AscI AvrII BaeI BcgI
BfrBI BmtI BsgI BsiWI BsmBI BsrGI BssHII BstZ17I
Bsu36I CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI