Restriction map for
pF1KE2630
(Circular) (Six-base) MAPSORT of: pF1KE2630.seq Check: 2145 from: 1 to: 5570
pF1KE2630 5570 bp
With 182 enzymes: SgfI *
June 3, 2018 14:24 ..
AccI GT'mk_AC
Cuts at: 2608 2608
Size: 5570
Acc65I G'GTAC_C
Cuts at: 2586 2586
Size: 5570
AclI AA'CG_TT
Cuts at: 1180 5410 1180
Size: 4230 1340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 682 760 1912 3395 3827 4574 4842 111
Size: 571 78 1152 1483 432 747 268 839
Fragments arranged by size:
1483 1152 839 747 571 432 268 78
AfeI AGC'GCT
Cuts at: 1043 1043
Size: 5570
AflIII A'CryG_T
Cuts at: 519 986 2922 4026 5003 519
Size: 467 1936 1104 977 1086
Fragments arranged by size:
1936 1104 1086 977 467
AgeI A'CCGG_T
Cuts at: 3958 3958
Size: 5570
AhdI GACnn_n'nnGTC
Cuts at: 2478 2478
Size: 5570
AleI CACnn'nnGTG
Cuts at: 1084 1084
Size: 5570
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 394 394
Size: 5570
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2056 2088 2056
Size: 32 5538
AlwNI CAG_nnn'CTG
Cuts at: 820 2142 2421 2875 4442 820
Size: 1322 279 454 1567 1948
Fragments arranged by size:
1948 1567 1322 454 279
ApaLI G'TGCA_C
Cuts at: 4340 4340
Size: 5570
ApoI r'AATT_y
Cuts at: 1219 1655 2355 2574 4923 5179 1219
Size: 436 700 219 2349 256 1610
Fragments arranged by size:
2349 1610 700 436 256 219
AseI AT'TA_AT
Cuts at: 20 20
Size: 5570
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5570
AvaI C'yCGr_G
Cuts at: 2590 2590
Size: 5570
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2122 2155 2122
Size: 33 5537
BamHI G'GATC_C
Cuts at: 2595 2595
Size: 5570
BanI G'GyrC_C
Cuts at: 192 2586 3000 3248 3283 192
Size: 2394 414 248 35 2479
Fragments arranged by size:
2479 2394 414 248 35
BanII G_rGCy'C
Cuts at: 2584 3614 2584
Size: 1030 4540
BbeI G_GCGC'C
Cuts at: 3004 3252 3004
Size: 248 5322
BbsI GAAGACnn'nnnn_
Cuts at: 585 585
Size: 5570
BbvCI CC'TCA_GC
Cuts at: 1473 2090 1473
Size: 617 4953
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5536
BciVI GTATCCnnnnn_n'
Cuts at: 3468 4229 5570 3468
Size: 761 1341 3468
Fragments arranged by size:
3468 1341 761
BclI T'GATC_A
Cuts at: 2186 3090 2186
Size: 904 4666
BglI GCCn_nnn'nGGC
Cuts at: 130 1012 130
Size: 882 4688
BglII A'GATC_T
Cuts at: 3085 5122 3085
Size: 2037 3533
BlpI GC'TnA_GC
Cuts at: 2682 2682
Size: 5570
Bme1580I G_kGCm'C
Cuts at: 2121 3195 3288 4344 2121
Size: 1074 93 1056 3347
Fragments arranged by size:
3347 1074 1056 93
BmgBI CAC'GTC
Cuts at: 504 2043 504
Size: 1539 4031
BmrI ACTGGGnnnn_n'
Cuts at: 1900 2782 2863 3198 5342 1900
Size: 882 81 335 2144 2128
Fragments arranged by size:
2144 2128 882 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3937 3937
Size: 5570
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 1326 1342 1622 183
Size: 1143 16 280 4131
Fragments arranged by size:
4131 1143 280 16
Bpu10I CC'TnA_GC
Cuts at: 1473 1808 2090 1473
Size: 335 282 4953
Fragments arranged by size:
4953 335 282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2746 3625 4117 4415 2746
Size: 879 492 298 3901
Fragments arranged by size:
3901 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 481 1084 5274 481
Size: 603 4190 777
Fragments arranged by size:
4190 777 603
BsaAI yAC'GTr
Cuts at: 483 987 2923 483
Size: 504 1936 3130
Fragments arranged by size:
3130 1936 504
BsaBI GATnn'nnATC
Cuts at: 6 3084 5121 6
Size: 3078 2037 455
Fragments arranged by size:
3078 2037 455
BsaHI Gr'CG_yC
Cuts at: 3001 3249 3951 5447 3001
Size: 248 702 1496 3124
Fragments arranged by size:
3124 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2759 3280 3958 4232 4379 4790 2759
Size: 521 678 274 147 411 3539
Fragments arranged by size:
3539 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 297 1701 297
Size: 1404 4166
BseYI C'CCAG_C
Cuts at: 566 883 1044 1072 2155 2292 2995 3753 4330
Size: 317 161 28 1083 137 703 758 577
Cuts at: 4330 4826 566
Size: 496 1310
Fragments arranged by size:
1310 1083 758 703 577 496 317 161
137 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 1543 2128 2279 400
Size: 1143 585 151 3691
Fragments arranged by size:
3691 1143 585 151
BsiEI CG_ry'CG
Cuts at: 104 2163 3112 3158 4366 104
Size: 2059 949 46 1208 1308
Fragments arranged by size:
2059 1308 1208 949 46
BsiHKAI G_wGCw'C
Cuts at: 1760 2584 3362 3552 4344 1760
Size: 824 778 190 792 2986
Fragments arranged by size:
2986 824 792 778 190
BsmI GAATG_Cn'
Cuts at: 2343 2343
Size: 5570
BsmBI CGTCTCn'nnnn_
Cuts at: 1963 1963
Size: 5570
Bsp1286I G_dGCh'C
Cuts at: 1760 2121 2584 3195 3288 3362 3552 3614 4344
Size: 361 463 611 93 74 190 62 730
Cuts at: 4344 1760
Size: 2986
Fragments arranged by size:
2986 730 611 463 361 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4790 4790
Size: 5570
BspHI T'CATG_A
Cuts at: 4746 4746
Size: 5570
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1515 2150 2620 2834 3136 3517 1515
Size: 635 470 214 302 381 3568
Fragments arranged by size:
3568 635 470 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1797 3862 3916 5400 5565 50
Size: 1747 2065 54 1484 165 55
Fragments arranged by size:
2065 1747 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1808 3482 1808
Size: 1674 3896
BsrFI r'CCGG_y
Cuts at: 1981 3568 3749 3958 1981
Size: 1587 181 209 3593
Fragments arranged by size:
3593 1587 209 181
BsrGI T'GTAC_A
Cuts at: 1427 1427
Size: 5570
BssSI C'ACGA_G
Cuts at: 3841 4199 3841
Size: 358 5212
BstAPI GCAn_nnn'nTGC
Cuts at: 2142 2947 2142
Size: 805 4765
BstBI TT'CG_AA
Cuts at: 3931 3931
Size: 5570
BstXI CCAn_nnnn'nTGG
Cuts at: 351 631 1170 1660 1689 351
Size: 280 539 490 29 4232
Fragments arranged by size:
4232 539 490 280 29
BstYI r'GATC_y
Cuts at: 1 2166 2595 3062 3085 3420 3666 4678 4764
Size: 2165 429 467 23 335 246 1012 86
Cuts at: 4764 4776 5122 1
Size: 12 346 449
Fragments arranged by size:
2165 1012 467 449 429 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2426 2426
Size: 5570
BtgI C'CryG_G
Cuts at: 207 420 1163 2102 207
Size: 213 743 939 3675
Fragments arranged by size:
3675 939 743 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3520 3701 3864 3520
Size: 181 163 5226
Fragments arranged by size:
5226 181 163
BtsI GCAGTG_nn'
Cuts at: 1493 1694 2321 2813 1493
Size: 201 627 492 4250
Fragments arranged by size:
4250 627 492 201
DraI TTT'AAA
Cuts at: 2569 2569
Size: 5570
DrdI GACnn_nn'nnGTC
Cuts at: 235 3276 4134 235
Size: 3041 858 1671
Fragments arranged by size:
3041 1671 858
EaeI y'GGCC_r
Cuts at: 260 789 1115 1565 1817 1853 1983 2160 3155
Size: 529 326 450 252 36 130 177 995
Cuts at: 3155 3329 3720 3747 5092 260
Size: 174 391 27 1345 738
Fragments arranged by size:
1345 995 738 529 450 391 326 252
177 174 130 36 27
EagI C'GGCC_G
Cuts at: 2160 3155 2160
Size: 995 4575
EarI CTCTTCn'nnn_
Cuts at: 1057 1654 2282 3593 3803 1057
Size: 597 628 1311 210 2824
Fragments arranged by size:
2824 1311 628 597 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3778 4088 4234 5380 3778
Size: 310 146 1146 3968
Fragments arranged by size:
3968 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2582 2582
Size: 5570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 183 682 760 1326 1342 1622 1912 3395
Size: 72 499 78 566 16 280 290 1483
Cuts at: 3395 3827 4574 4842 111
Size: 432 747 268 839
Fragments arranged by size:
1483 839 747 566 499 432 290 280
268 78 72 16
EcoNI CCTnn'n_nnAGG
Cuts at: 274 274
Size: 5570
EcoO109I rG'GnC_Cy
Cuts at: 771 1884 2241 2709 771
Size: 1113 357 468 3632
Fragments arranged by size:
3632 1113 468 357
EcoRI G'AATT_C
Cuts at: 2355 2574 2355
Size: 219 5351
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3589 3589
Size: 5570
FspI TGC'GCA
Cuts at: 330 3351 330
Size: 3021 2549
HaeII r_GCGC'y
Cuts at: 190 206 660 1045 3004 3252 4274 5253 190
Size: 16 454 385 1959 248 1022 979 507
Fragments arranged by size:
1959 1022 979 507 454 385 248 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 575 607 819 851 1936 1968 2464 2496 3412
Size: 32 212 32 1085 32 496 32 916
Cuts at: 3412 3442 3444 3474 575
Size: 30 2 30 2671
Fragments arranged by size:
2671 1085 916 496 212 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1098 2609 1098
Size: 1511 4059
KasI G'GCGC_C
Cuts at: 3000 3248 3000
Size: 248 5322
KpnI G_GTAC'C
Cuts at: 2590 2590
Size: 5570
MluI A'CGCG_T
Cuts at: 5003 5003
Size: 5570
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4241 4425 4241
Size: 184 5386
MscI TGG'CCA
Cuts at: 262 1567 1819 3331 262
Size: 1305 252 1512 2501
Fragments arranged by size:
2501 1512 1305 252
MslI CAynn'nnrTG
Cuts at: 1057 1084 1664 3559 3686 1057
Size: 27 580 1895 127 2941
Fragments arranged by size:
2941 1895 580 127 27
MspA1I CmG'CkG
Cuts at: 351 883 891 1131 2159 2681 2995 3355 4368
Size: 532 8 240 1028 522 314 360 1013
Cuts at: 4368 4613 351
Size: 245 1308
Fragments arranged by size:
1308 1028 1013 532 522 360 314 245
240 8
NaeI GCC'GGC
Cuts at: 3751 3751
Size: 5570
NarI GG'CG_CC
Cuts at: 3001 3249 3001
Size: 248 5322
NcoI C'CATG_G
Cuts at: 1163 1163
Size: 5570
NdeI CA'TA_TG
Cuts at: 2271 2271
Size: 5570
NgoMIV G'CCGG_C
Cuts at: 3749 3749
Size: 5570
NspI r_CATG'y
Cuts at: 523 2623 3123 4030 523
Size: 2100 500 907 2063
Fragments arranged by size:
2100 2063 907 500
PasI CC'CwG_GG
Cuts at: 148 148
Size: 5570
PciI A'CATG_T
Cuts at: 519 4026 519
Size: 3507 2063
PflMI CCAn_nnn'nTGG
Cuts at: 1164 1488 1563 1164
Size: 324 75 5171
Fragments arranged by size:
5171 324 75
PfoI T'CCnGG_A
Cuts at: 926 959 1211 1609 926
Size: 33 252 398 4887
Fragments arranged by size:
4887 398 252 33
PmeI GTTT'AAAC
Cuts at: 2569 2569
Size: 5570
PmlI CAC'GTG
Cuts at: 483 483
Size: 5570
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4709 4741 4709
Size: 32 5538
PpuMI rG'GwC_Cy
Cuts at: 771 1884 2241 771
Size: 1113 357 4100
Fragments arranged by size:
4100 1113 357
PstI C_TGCA'G
Cuts at: 2617 3302 2617
Size: 685 4885
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5570
PvuII CAG'CTG
Cuts at: 351 883 891 1131 2995 3355 351
Size: 532 8 240 1864 360 2566
Fragments arranged by size:
2566 1864 532 360 240 8
RsrII CG'GwC_CG
Cuts at: 3765 3765
Size: 5570
SacI G_AGCT'C
Cuts at: 2584 2584
Size: 5570
SalI G'TCGA_C
Cuts at: 2607 2607
Size: 5570
SanDI GG'GwC_CC
Cuts at: 2241 2241
Size: 5570
SapI GCTCTTCn'nnn_
Cuts at: 1057 2282 3593 3803 1057
Size: 1225 1311 210 2824
Fragments arranged by size:
2824 1311 1225 210
SbfI CC_TGCA'GG
Cuts at: 2617 2617
Size: 5570
ScaI AGT'ACT
Cuts at: 5087 5087
Size: 5570
SexAI A'CCwGG_T
Cuts at: 2066 2066
Size: 5570
SfcI C'TryA_G
Cuts at: 33 812 2387 2613 3298 4291 4482 33
Size: 779 1575 226 685 993 191 1121
Fragments arranged by size:
1575 1121 993 779 685 226 191
SfoI GGC'GCC
Cuts at: 3002 3250 3002
Size: 248 5322
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5570
SmaI CCC'GGG
Cuts at: 2592 2592
Size: 5570
SmlI C'TyrA_G
Cuts at: 2725 3640 4132 4394 2725
Size: 915 492 262 3901
Fragments arranged by size:
3901 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5570
SphI G_CATG'C
Cuts at: 2623 3123 2623
Size: 500 5070
SspI AAT'ATT
Cuts at: 2380 2380
Size: 5570
StuI AGG'CCT
Cuts at: 918 918
Size: 5570
StyI C'CwwG_G
Cuts at: 606 786 1163 2704 3058 606
Size: 180 377 1541 354 3118
Fragments arranged by size:
3118 1541 377 354 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2032 3153 3286 3745 3954 2032
Size: 1121 133 459 209 3648
Fragments arranged by size:
3648 1121 459 209 133
TatI w'GTAC_w
Cuts at: 1427 5085 1427
Size: 3658 1912
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5087 5119 5087
Size: 32 5538
Tth111I GACn'n_nGTC
Cuts at: 3367 3367
Size: 5570
XbaI T'CTAG_A
Cuts at: 2601 2601
Size: 5570
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1877 3065 1877
Size: 1188 4382
XmaI C'CCGG_G
Cuts at: 2590 2590
Size: 5570
XmnI GAAnn'nnTTC
Cuts at: 1180 1180
Size: 5570
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII ApaI AscI AvrII BfrBI BmtI
BsaXI BsiWI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PshAI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI