Restriction map for
pF1KE2618
(Circular) (Six-base) MAPSORT of: pF1KE2618.seq Check: 3558 from: 1 to: 4292
pF1KE2618 4292 bp
With 182 enzymes: SgfI *
April 12, 2016 09:51 ..
AatII G_ACGT'C
Cuts at: 1244 1244
Size: 4292
AccI GT'mk_AC
Cuts at: 515 1330 515
Size: 815 3477
Acc65I G'GTAC_C
Cuts at: 687 1308 687
Size: 621 3671
AclI AA'CG_TT
Cuts at: 4132 4132
Size: 4292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1150 1176 2117 2549 3296 3564 439
Size: 711 26 941 432 747 268 1167
Fragments arranged by size:
1167 941 747 711 432 268 26
AflIII A'CryG_T
Cuts at: 359 1644 2748 3725 359
Size: 1285 1104 977 926
Fragments arranged by size:
1285 1104 977 926
AgeI A'CCGG_T
Cuts at: 2680 2680
Size: 4292
AleI CACnn'nnGTG
Cuts at: 541 541
Size: 4292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 465 465
Size: 4292
AlwNI CAG_nnn'CTG
Cuts at: 1597 3164 1597
Size: 1567 2725
ApaLI G'TGCA_C
Cuts at: 3062 3062
Size: 4292
ApoI r'AATT_y
Cuts at: 580 718 1296 3645 3901 580
Size: 138 578 2349 256 971
Fragments arranged by size:
2349 971 578 256 138
AseI AT'TA_AT
Cuts at: 20 20
Size: 4292
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
AvaI C'yCGr_G
Cuts at: 1312 1312
Size: 4292
BamHI G'GATC_C
Cuts at: 1317 1317
Size: 4292
BanI G'GyrC_C
Cuts at: 258 554 687 1308 1722 1970 2005 258
Size: 296 133 621 414 248 35 2545
Fragments arranged by size:
2545 621 414 296 248 133 35
BanII G_rGCy'C
Cuts at: 173 1214 1306 2336 173
Size: 1041 92 1030 2129
Fragments arranged by size:
2129 1041 1030 92
BbeI G_GCGC'C
Cuts at: 262 1726 1974 262
Size: 1464 248 2580
Fragments arranged by size:
2580 1464 248
BbsI GAAGACnn'nnnn_
Cuts at: 678 1236 678
Size: 558 3734
BbvCI CC'TCA_GC
Cuts at: 1109 1109
Size: 4292
BciVI GTATCCnnnnn_n'
Cuts at: 988 2190 2951 4292 988
Size: 1202 761 1341 988
Fragments arranged by size:
1341 1202 988 761
BclI T'GATC_A
Cuts at: 1812 1812
Size: 4292
BglI GCCn_nnn'nGGC
Cuts at: 391 391
Size: 4292
BglII A'GATC_T
Cuts at: 1807 3844 1807
Size: 2037 2255
BlpI GC'TnA_GC
Cuts at: 527 571 1404 527
Size: 44 833 3415
Fragments arranged by size:
3415 833 44
Bme1580I G_kGCm'C
Cuts at: 559 1917 2010 3066 559
Size: 1358 93 1056 1785
Fragments arranged by size:
1785 1358 1056 93
BmgBI CAC'GTC
Cuts at: 360 534 360
Size: 174 4118
BmrI ACTGGGnnnn_n'
Cuts at: 639 1279 1504 1585 1920 4064 639
Size: 640 225 81 335 2144 867
Fragments arranged by size:
2144 867 640 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1241 2659 1241
Size: 1418 2874
Bpu10I CC'TnA_GC
Cuts at: 1109 1109
Size: 4292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1468 2347 2839 3137 1468
Size: 879 492 298 2623
Fragments arranged by size:
2623 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3996 3996
Size: 4292
BsaAI yAC'GTr
Cuts at: 1645 1645
Size: 4292
BsaBI GATnn'nnATC
Cuts at: 6 1806 3843 6
Size: 1800 2037 455
Fragments arranged by size:
2037 1800 455
BsaHI Gr'CG_yC
Cuts at: 259 1241 1723 1971 2673 4169 259
Size: 982 482 248 702 1496 382
Fragments arranged by size:
1496 982 702 482 382 248
BsaWI w'CCGG_w
Cuts at: 1481 2002 2680 2954 3101 3512 1481
Size: 521 678 274 147 411 2261
Fragments arranged by size:
2261 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 465 495 465
Size: 30 4262
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 198 473 198
Size: 275 4017
BseYI C'CCAG_C
Cuts at: 280 1717 2475 3052 3548 280
Size: 1437 758 577 496 1024
Fragments arranged by size:
1437 1024 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1834 1880 3088 104
Size: 1730 46 1208 1308
Fragments arranged by size:
1730 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 382 533 1306 2084 2274 3066 382
Size: 151 773 778 190 792 1608
Fragments arranged by size:
1608 792 778 773 190 151
Bsp1286I G_dGCh'C
Cuts at: 173 382 533 559 1214 1306 1917 2010 2084
Size: 209 151 26 655 92 611 93 74
Cuts at: 2084 2274 2336 3066 173
Size: 190 62 730 1399
Fragments arranged by size:
1399 730 655 611 209 190 151 93
92 74 62 26
BspEI T'CCGG_A
Cuts at: 3512 3512
Size: 4292
BspHI T'CATG_A
Cuts at: 3468 3468
Size: 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1342 1556 1858 2239 1342
Size: 214 302 381 3395
Fragments arranged by size:
3395 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2584 2638 4122 4287 50
Size: 2534 54 1484 165 55
Fragments arranged by size:
2534 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2204 2204
Size: 4292
BsrFI r'CCGG_y
Cuts at: 2290 2471 2680 2290
Size: 181 209 3902
Fragments arranged by size:
3902 209 181
BsrGI T'GTAC_A
Cuts at: 734 734
Size: 4292
BssSI C'ACGA_G
Cuts at: 2563 2921 2563
Size: 358 3934
BstAPI GCAn_nnn'nTGC
Cuts at: 1669 1669
Size: 4292
BstBI TT'CG_AA
Cuts at: 2653 2653
Size: 4292
BstYI r'GATC_y
Cuts at: 1 657 854 1006 1317 1784 1807 2142 2388
Size: 656 197 152 311 467 23 335 246
Cuts at: 2388 3400 3486 3498 3844 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 656 467 449 346 335 311 246
197 152 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 485 485
Size: 4292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2242 2423 2586 2242
Size: 181 163 3948
Fragments arranged by size:
3948 181 163
BtsI GCAGTG_nn'
Cuts at: 1535 1535
Size: 4292
DraI TTT'AAA
Cuts at: 1291 1291
Size: 4292
DrdI GACnn_nn'nnGTC
Cuts at: 1998 2856 1998
Size: 858 3434
EaeI y'GGCC_r
Cuts at: 392 1877 2051 2442 2469 3814 392
Size: 1485 174 391 27 1345 870
Fragments arranged by size:
1485 1345 870 391 174 27
EagI C'GGCC_G
Cuts at: 1877 1877
Size: 4292
EarI CTCTTCn'nnn_
Cuts at: 867 2315 2525 867
Size: 1448 210 2634
Fragments arranged by size:
2634 1448 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 901 2500 2810 2956 4102 901
Size: 1599 310 146 1146 1091
Fragments arranged by size:
1599 1146 1091 310 146
EcoICRI GAG'CTC
Cuts at: 1304 1304
Size: 4292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1150 1176 2117 2549 3296 3564 439
Size: 711 26 941 432 747 268 1167
Fragments arranged by size:
1167 941 747 711 432 268 26
EcoNI CCTnn'n_nnAGG
Cuts at: 1191 1191
Size: 4292
EcoO109I rG'GnC_Cy
Cuts at: 1431 1431
Size: 4292
EcoRI G'AATT_C
Cuts at: 580 718 1296 580
Size: 138 578 3576
Fragments arranged by size:
3576 578 138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 4292
FspI TGC'GCA
Cuts at: 2073 2073
Size: 4292
HaeII r_GCGC'y
Cuts at: 262 954 1726 1974 2996 3975 262
Size: 692 772 248 1022 979 579
Fragments arranged by size:
1022 979 772 692 579 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 465 482 497 514 588 620 2134
Size: 32 147 17 15 17 74 32 1514
Cuts at: 2134 2164 2166 2196 286
Size: 30 2 30 2382
Fragments arranged by size:
2382 1514 147 74 32 32 30 30
17 17 15 2
HincII GTy'rAC
Cuts at: 1331 1331
Size: 4292
KasI G'GCGC_C
Cuts at: 258 1722 1970 258
Size: 1464 248 2580
Fragments arranged by size:
2580 1464 248
KpnI G_GTAC'C
Cuts at: 691 1312 691
Size: 621 3671
MfeI C'AATT_G
Cuts at: 1118 1118
Size: 4292
MluI A'CGCG_T
Cuts at: 3725 3725
Size: 4292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 350 744 2963 3147 350
Size: 394 2219 184 1495
Fragments arranged by size:
2219 1495 394 184
MscI TGG'CCA
Cuts at: 2053 2053
Size: 4292
MslI CAynn'nnrTG
Cuts at: 541 2281 2408 541
Size: 1740 127 2425
Fragments arranged by size:
2425 1740 127
MspA1I CmG'CkG
Cuts at: 1403 1717 2077 3090 3335 1403
Size: 314 360 1013 245 2360
Fragments arranged by size:
2360 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2473 2473
Size: 4292
NarI GG'CG_CC
Cuts at: 259 1723 1971 259
Size: 1464 248 2580
Fragments arranged by size:
2580 1464 248
NgoMIV G'CCGG_C
Cuts at: 2471 2471
Size: 4292
NspI r_CATG'y
Cuts at: 452 1345 1845 2752 452
Size: 893 500 907 1992
Fragments arranged by size:
1992 907 893 500
PciI A'CATG_T
Cuts at: 2748 2748
Size: 4292
PmeI GTTT'AAAC
Cuts at: 1291 1291
Size: 4292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 193 225 3431 3463 193
Size: 32 3206 32 1022
Fragments arranged by size:
3206 1022 32 32
PstI C_TGCA'G
Cuts at: 1339 2024 1339
Size: 685 3607
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4292
PvuII CAG'CTG
Cuts at: 1717 2077 1717
Size: 360 3932
RsrII CG'GwC_CG
Cuts at: 2487 2487
Size: 4292
SacI G_AGCT'C
Cuts at: 1306 1306
Size: 4292
SalI G'TCGA_C
Cuts at: 1329 1329
Size: 4292
SapI GCTCTTCn'nnn_
Cuts at: 2315 2525 2315
Size: 210 4082
SbfI CC_TGCA'GG
Cuts at: 1339 1339
Size: 4292
ScaI AGT'ACT
Cuts at: 3809 3809
Size: 4292
SfcI C'TryA_G
Cuts at: 33 1335 2020 3013 3204 33
Size: 1302 685 993 191 1121
Fragments arranged by size:
1302 1121 993 685 191
SfoI GGC'GCC
Cuts at: 260 1724 1972 260
Size: 1464 248 2580
Fragments arranged by size:
2580 1464 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
SmaI CCC'GGG
Cuts at: 1314 1314
Size: 4292
SmlI C'TyrA_G
Cuts at: 1447 2362 2854 3116 1447
Size: 915 492 262 2623
Fragments arranged by size:
2623 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4292
SphI G_CATG'C
Cuts at: 1345 1845 1345
Size: 500 3792
StyI C'CwwG_G
Cuts at: 1426 1780 1426
Size: 354 3938
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1875 2008 2467 2676 1875
Size: 133 459 209 3491
Fragments arranged by size:
3491 459 209 133
TatI w'GTAC_w
Cuts at: 155 734 925 3807 155
Size: 579 191 2882 640
Fragments arranged by size:
2882 640 579 191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 302 334 962 994 3809 3841 302
Size: 32 628 32 2815 32 753
Fragments arranged by size:
2815 753 628 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2089 2089
Size: 4292
XbaI T'CTAG_A
Cuts at: 1323 1323
Size: 4292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1787 114
Size: 1673 2619
XmaI C'CCGG_G
Cuts at: 1312 1312
Size: 4292
ZraI GAC'GTC
Cuts at: 1242 1242
Size: 4292
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BmtI BpmI BsgI BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI