Restriction map for
pF1KE2607
(Circular) (Six-base) MAPSORT of: pF1KE2607.seq Check: 719 from: 1 to: 6425
pF1KE2607 6425 bp
With 182 enzymes: SgfI *
September 11, 2017 11:55 ..
AccI GT'mk_AC
Cuts at: 2258 3463 2258
Size: 1205 5220
Acc65I G'GTAC_C
Cuts at: 2869 3441 2869
Size: 572 5853
AclI AA'CG_TT
Cuts at: 6265 6265
Size: 6425
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 585 598 1338 1417 1593 1801 1921 4250 4682
Size: 13 740 79 176 208 120 2329 432
Cuts at: 4682 5429 5697 585
Size: 747 268 1313
Fragments arranged by size:
2329 1313 747 740 432 268 208 176
120 79 13
AfeI AGC'GCT
Cuts at: 2791 2791
Size: 6425
AflIII A'CryG_T
Cuts at: 1763 2996 3777 4881 5858 1763
Size: 1233 781 1104 977 2330
Fragments arranged by size:
2330 1233 1104 977 781
AgeI A'CCGG_T
Cuts at: 4813 4813
Size: 6425
AleI CACnn'nnGTG
Cuts at: 1618 1618
Size: 6425
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2793 2825 2793
Size: 32 6393
AlwNI CAG_nnn'CTG
Cuts at: 1865 2037 3730 5297 1865
Size: 172 1693 1567 2993
Fragments arranged by size:
2993 1693 1567 172
ApaLI G'TGCA_C
Cuts at: 5195 5195
Size: 6425
ApoI r'AATT_y
Cuts at: 234 249 397 1076 1256 1647 2133 3429 5778
Size: 15 148 679 180 391 486 1296 2349
Cuts at: 5778 6034 234
Size: 256 625
Fragments arranged by size:
2349 1296 679 625 486 391 256 180
148 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 6425
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6425
AvaI C'yCGr_G
Cuts at: 1101 1976 2168 2407 2962 3445 1101
Size: 875 192 239 555 483 4081
Fragments arranged by size:
4081 875 555 483 239 192
BamHI G'GATC_C
Cuts at: 2768 3450 2768
Size: 682 5743
BanI G'GyrC_C
Cuts at: 346 518 559 655 1781 2394 2850 2869 3058
Size: 172 41 96 1126 613 456 19 189
Cuts at: 3058 3441 3855 4103 4138 346
Size: 383 414 248 35 2633
Fragments arranged by size:
2633 1126 613 456 414 383 248 189
172 96 41 35 19
BanII G_rGCy'C
Cuts at: 2828 3439 4469 2828
Size: 611 1030 4784
Fragments arranged by size:
4784 1030 611
BbeI G_GCGC'C
Cuts at: 563 3859 4107 563
Size: 3296 248 2881
Fragments arranged by size:
3296 2881 248
BbsI GAAGACnn'nnnn_
Cuts at: 264 1727 1909 2024 2310 2328 3150 3218 3257
Size: 1463 182 115 286 18 822 68 39
Cuts at: 3257 3382 264
Size: 125 3307
Fragments arranged by size:
3307 1463 822 286 182 125 115 68
39 18
BbvCI CC'TCA_GC
Cuts at: 803 803
Size: 6425
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1915 1949 1915
Size: 34 6391
BciVI GTATCCnnnnn_n'
Cuts at: 3357 4323 5084 6425 3357
Size: 966 761 1341 3357
Fragments arranged by size:
3357 1341 966 761
BclI T'GATC_A
Cuts at: 3945 3945
Size: 6425
BglII A'GATC_T
Cuts at: 596 3940 5977 596
Size: 3344 2037 1044
Fragments arranged by size:
3344 2037 1044
BlpI GC'TnA_GC
Cuts at: 130 3537 130
Size: 3407 3018
Bme1580I G_kGCm'C
Cuts at: 2855 3061 4050 4143 5199 2855
Size: 206 989 93 1056 4081
Fragments arranged by size:
4081 1056 989 206 93
BmgBI CAC'GTC
Cuts at: 1764 2862 1764
Size: 1098 5327
BmrI ACTGGGnnnn_n'
Cuts at: 448 816 2849 2912 3637 3718 4053 6197 448
Size: 368 2033 63 725 81 335 2144 676
Fragments arranged by size:
2144 2033 725 676 368 335 81 63
BmtI G_CTAG'C
Cuts at: 1130 1130
Size: 6425
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4792 4792
Size: 6425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 1429 1561 2098 2814 168
Size: 1261 132 537 716 3779
Fragments arranged by size:
3779 1261 716 537 132
Bpu10I CC'TnA_GC
Cuts at: 803 803
Size: 6425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3367 3601 4480 4972 5270 3367
Size: 234 879 492 298 4522
Fragments arranged by size:
4522 879 492 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 771 1966 6129 771
Size: 1195 4163 1067
Fragments arranged by size:
4163 1195 1067
BsaAI yAC'GTr
Cuts at: 2370 2469 2876 3778 2370
Size: 99 407 902 5017
Fragments arranged by size:
5017 902 407 99
BsaBI GATnn'nnATC
Cuts at: 6 2698 2974 3939 5976 6
Size: 2692 276 965 2037 455
Fragments arranged by size:
2692 2037 965 455 276
BsaHI Gr'CG_yC
Cuts at: 410 560 3856 4104 4806 6302 410
Size: 150 3296 248 702 1496 533
Fragments arranged by size:
3296 1496 702 533 248 150
BsaWI w'CCGG_w
Cuts at: 938 1625 1712 3614 4135 4813 5087 5234 5645
Size: 687 87 1902 521 678 274 147 411
Cuts at: 5645 938
Size: 1718
Fragments arranged by size:
1902 1718 687 678 521 411 274 147
87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 292 322 292
Size: 30 6395
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 739 1234 1237 1657 1966 739
Size: 495 3 420 309 5198
Fragments arranged by size:
5198 495 420 309 3
BseYI C'CCAG_C
Cuts at: 509 1303 1939 2117 2517 3850 4608 5185 5681
Size: 794 636 178 400 1333 758 577 496
Cuts at: 5681 509
Size: 1253
Fragments arranged by size:
1333 1253 794 758 636 577 496 400
178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1851 2877 3133 1851
Size: 1026 256 5143
Fragments arranged by size:
5143 1026 256
BsiEI CG_ry'CG
Cuts at: 104 3967 4013 5221 104
Size: 3863 46 1208 1308
Fragments arranged by size:
3863 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 202 2945 3439 4217 4407 5199 202
Size: 2743 494 778 190 792 1428
Fragments arranged by size:
2743 1428 792 778 494 190
BsmBI CGTCTCn'nnnn_
Cuts at: 197 197
Size: 6425
Bsp1286I G_dGCh'C
Cuts at: 202 2828 2855 2945 3061 3439 4050 4143 4217
Size: 2626 27 90 116 378 611 93 74
Cuts at: 4217 4407 4469 5199 202
Size: 190 62 730 1428
Fragments arranged by size:
2626 1428 730 611 378 190 116 93
90 74 62 27
BspEI T'CCGG_A
Cuts at: 938 1712 5645 938
Size: 774 3933 1718
Fragments arranged by size:
3933 1718 774
BspHI T'CATG_A
Cuts at: 3281 5601 3281
Size: 2320 4105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2957 3035 3475 3689 3991 4372 2957
Size: 78 440 214 302 381 5010
Fragments arranged by size:
5010 440 381 302 214 78
BsrBI CCG'CTC
Cuts at: 50 4717 4771 6255 6420 50
Size: 4667 54 1484 165 55
Fragments arranged by size:
4667 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1329 2155 4337 1329
Size: 826 2182 3417
Fragments arranged by size:
3417 2182 826
BsrFI r'CCGG_y
Cuts at: 605 4423 4604 4813 605
Size: 3818 181 209 2217
Fragments arranged by size:
3818 2217 209 181
BssSI C'ACGA_G
Cuts at: 892 4696 5054 892
Size: 3804 358 2263
Fragments arranged by size:
3804 2263 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1788 2694 2949 3802 1788
Size: 906 255 853 4411
Fragments arranged by size:
4411 906 853 255
BstBI TT'CG_AA
Cuts at: 4786 4786
Size: 6425
BstXI CCAn_nnnn'nTGG
Cuts at: 1030 2862 2982 1030
Size: 1832 120 4473
Fragments arranged by size:
4473 1832 120
BstYI r'GATC_y
Cuts at: 1 596 849 1001 2768 3450 3917 3940 4275
Size: 595 253 152 1767 682 467 23 335
Cuts at: 4275 4521 5533 5619 5631 5977 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1767 1012 682 595 467 449 346 335
253 246 152 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1217 2773 1217
Size: 1556 4869
BtgI C'CryG_G
Cuts at: 296 296
Size: 6425
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 603 812 2027 2097 2328 4375 4556 4719 603
Size: 209 1215 70 231 2047 181 163 2309
Fragments arranged by size:
2309 2047 1215 231 209 181 163 70
BtsI GCAGTG_nn'
Cuts at: 2889 3668 2889
Size: 779 5646
ClaI AT'CG_AT
Cuts at: 2702 3348 2702
Size: 646 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 975 1010 975
Size: 35 6390
DraI TTT'AAA
Cuts at: 3424 3424
Size: 6425
DraIII CAC_nnn'GTG
Cuts at: 1764 2000 2926 1764
Size: 236 926 5263
Fragments arranged by size:
5263 926 236
DrdI GACnn_nn'nnGTC
Cuts at: 4131 4989 4131
Size: 858 5567
EaeI y'GGCC_r
Cuts at: 737 1731 1910 2756 2834 2844 4010 4184 4575
Size: 994 179 846 78 10 1166 174 391
Cuts at: 4575 4602 5947 737
Size: 27 1345 1215
Fragments arranged by size:
1345 1215 1166 994 846 391 179 174
78 27 10
EagI C'GGCC_G
Cuts at: 4010 4010
Size: 6425
EarI CTCTTCn'nnn_
Cuts at: 1600 1698 1804 2446 2568 4448 4658 1600
Size: 98 106 642 122 1880 210 3367
Fragments arranged by size:
3367 1880 642 210 122 106 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1519 4633 4943 5089 6235 1519
Size: 3114 310 146 1146 1709
Fragments arranged by size:
3114 1709 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3437 3437
Size: 6425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 585 598 1338 1417 1429 1561 1593 1801
Size: 417 13 740 79 12 132 32 208
Cuts at: 1801 1921 2098 2814 4250 4682 5429 5697 168
Size: 120 177 716 1436 432 747 268 896
Fragments arranged by size:
1436 896 747 740 716 432 417 268
208 177 132 120 79 32 13 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1135 3112 1135
Size: 1977 4448
EcoO109I rG'GnC_Cy
Cuts at: 2668 3564 2668
Size: 896 5529
EcoRI G'AATT_C
Cuts at: 1647 3429 1647
Size: 1782 4643
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2788 3207 4444 2788
Size: 419 1237 4769
Fragments arranged by size:
4769 1237 419
FspI TGC'GCA
Cuts at: 592 4206 592
Size: 3614 2811
HaeII r_GCGC'y
Cuts at: 563 2793 3859 4107 5129 6108 563
Size: 2230 1066 248 1022 979 880
Fragments arranged by size:
2230 1066 1022 979 880 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 254 286 4267 4297 4299 4329 254
Size: 32 3981 30 2 30 2350
Fragments arranged by size:
3981 2350 32 30 30 2
HincII GTy'rAC
Cuts at: 2259 3024 3464 2259
Size: 765 440 5220
Fragments arranged by size:
5220 765 440
HindIII A'AGCT_T
Cuts at: 1399 1894 2054 3370 1399
Size: 495 160 1316 4454
Fragments arranged by size:
4454 1316 495 160
KasI G'GCGC_C
Cuts at: 559 3855 4103 559
Size: 3296 248 2881
Fragments arranged by size:
3296 2881 248
KpnI G_GTAC'C
Cuts at: 2873 3445 2873
Size: 572 5853
MluI A'CGCG_T
Cuts at: 5858 5858
Size: 6425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 794 1010 1216 1625 5096 5280 794
Size: 216 206 409 3471 184 1939
Fragments arranged by size:
3471 1939 409 216 206 184
MscI TGG'CCA
Cuts at: 739 1912 2758 2836 4186 739
Size: 1173 846 78 1350 2978
Fragments arranged by size:
2978 1350 1173 846 78
MslI CAynn'nnrTG
Cuts at: 547 1618 2995 3178 4414 4541 547
Size: 1071 1377 183 1236 127 2431
Fragments arranged by size:
2431 1377 1236 1071 183 127
MspA1I CmG'CkG
Cuts at: 1395 1422 2034 3536 3850 4210 5223 5468 1395
Size: 27 612 1502 314 360 1013 245 2352
Fragments arranged by size:
2352 1502 1013 612 360 314 245 27
NaeI GCC'GGC
Cuts at: 607 4606 607
Size: 3999 2426
NarI GG'CG_CC
Cuts at: 560 3856 4104 560
Size: 3296 248 2881
Fragments arranged by size:
3296 2881 248
NgoMIV G'CCGG_C
Cuts at: 605 4604 605
Size: 3999 2426
NheI G'CTAG_C
Cuts at: 1126 1126
Size: 6425
NspI r_CATG'y
Cuts at: 591 3000 3478 3978 4885 591
Size: 2409 478 500 907 2131
Fragments arranged by size:
2409 2131 907 500 478
PasI CC'CwG_GG
Cuts at: 1881 1881
Size: 6425
PciI A'CATG_T
Cuts at: 2996 4881 2996
Size: 1885 4540
PflMI CCAn_nnn'nTGG
Cuts at: 339 2183 339
Size: 1844 4581
PfoI T'CCnGG_A
Cuts at: 863 2323 3065 863
Size: 1460 742 4223
Fragments arranged by size:
4223 1460 742
PmeI GTTT'AAAC
Cuts at: 3424 3424
Size: 6425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5564 5596 5564
Size: 32 6393
PpuMI rG'GwC_Cy
Cuts at: 2668 2668
Size: 6425
PsiI TTA'TAA
Cuts at: 2680 2680
Size: 6425
PstI C_TGCA'G
Cuts at: 1637 2690 3127 3472 4157 1637
Size: 1053 437 345 685 3905
Fragments arranged by size:
3905 1053 685 437 345
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6425
PvuII CAG'CTG
Cuts at: 1395 1422 2034 3850 4210 1395
Size: 27 612 1816 360 3610
Fragments arranged by size:
3610 1816 612 360 27
RsrII CG'GwC_CG
Cuts at: 4620 4620
Size: 6425
SacI G_AGCT'C
Cuts at: 3439 3439
Size: 6425
SalI G'TCGA_C
Cuts at: 2257 3462 2257
Size: 1205 5220
SapI GCTCTTCn'nnn_
Cuts at: 4448 4658 4448
Size: 210 6215
SbfI CC_TGCA'GG
Cuts at: 3472 3472
Size: 6425
ScaI AGT'ACT
Cuts at: 3121 5942 3121
Size: 2821 3604
SexAI A'CCwGG_T
Cuts at: 536 3080 536
Size: 2544 3881
SfcI C'TryA_G
Cuts at: 33 1633 2686 3123 3468 4153 5146 5337 33
Size: 1600 1053 437 345 685 993 191 1121
Fragments arranged by size:
1600 1121 1053 993 685 437 345 191
SfoI GGC'GCC
Cuts at: 561 3857 4105 561
Size: 3296 248 2881
Fragments arranged by size:
3296 2881 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6425
SmaI CCC'GGG
Cuts at: 1978 3447 1978
Size: 1469 4956
SmlI C'TyrA_G
Cuts at: 3382 3580 4495 4987 5249 3382
Size: 198 915 492 262 4558
Fragments arranged by size:
4558 915 492 262 198
SnaBI TAC'GTA
Cuts at: 2469 2469
Size: 6425
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6425
SphI G_CATG'C
Cuts at: 591 3478 3978 591
Size: 2887 500 3038
Fragments arranged by size:
3038 2887 500
SspI AAT'ATT
Cuts at: 732 732
Size: 6425
StyI C'CwwG_G
Cuts at: 290 392 1023 2216 3559 3913 290
Size: 102 631 1193 1343 354 2802
Fragments arranged by size:
2802 1343 1193 631 354 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 364 546 673 1588 2802 4008 4141 4600 4809
Size: 182 127 915 1214 1206 133 459 209
Cuts at: 4809 364
Size: 1980
Fragments arranged by size:
1980 1214 1206 915 459 209 182 133
127
TatI w'GTAC_w
Cuts at: 179 677 2420 3119 5940 179
Size: 498 1743 699 2821 664
Fragments arranged by size:
2821 1743 699 664 498
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 292 324 834 845 866 877 5942 5974 292
Size: 32 510 11 21 11 5065 32 743
Fragments arranged by size:
5065 743 510 32 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 3082 4222 3082
Size: 1140 5285
XbaI T'CTAG_A
Cuts at: 3456 3456
Size: 6425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3015 3920 3015
Size: 905 5520
XmaI C'CCGG_G
Cuts at: 1976 3445 1976
Size: 1469 4956
XmnI GAAnn'nnTTC
Cuts at: 3190 3190
Size: 6425
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI ApaI AscI AvrII
BaeI BfrBI BglI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NotI NruI NsiI PacI PmlI PshAI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SrfI StuI SwaI
XhoI ZraI