Restriction map for
pF1KE2600
(Circular) (Six-base) MAPSORT of: pF1KE2600.seq Check: 8123 from: 1 to: 4004
pF1KE2600 4004 bp
With 182 enzymes: SgfI *
January 12, 2016 10:05 ..
AccI GT'mk_AC
Cuts at: 1042 1042
Size: 4004
Acc65I G'GTAC_C
Cuts at: 1020 1020
Size: 4004
AclI AA'CG_TT
Cuts at: 3844 3844
Size: 4004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1829 2261 3008 3276 817
Size: 1012 432 747 268 1545
Fragments arranged by size:
1545 1012 747 432 268
AfeI AGC'GCT
Cuts at: 367 679 367
Size: 312 3692
AflIII A'CryG_T
Cuts at: 1356 2460 3437 1356
Size: 1104 977 1923
Fragments arranged by size:
1923 1104 977
AgeI A'CCGG_T
Cuts at: 2392 2392
Size: 4004
AhdI GACnn_n'nnGTC
Cuts at: 696 696
Size: 4004
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 712 744 712
Size: 32 3972
AlwNI CAG_nnn'CTG
Cuts at: 426 1309 2876 426
Size: 883 1567 1554
Fragments arranged by size:
1567 1554 883
ApaLI G'TGCA_C
Cuts at: 245 973 2774 245
Size: 728 1801 1475
Fragments arranged by size:
1801 1475 728
ApoI r'AATT_y
Cuts at: 1008 3357 3613 1008
Size: 2349 256 1399
Fragments arranged by size:
2349 1399 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4004
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
AvaI C'yCGr_G
Cuts at: 198 1024 198
Size: 826 3178
BamHI G'GATC_C
Cuts at: 1029 1029
Size: 4004
BanI G'GyrC_C
Cuts at: 1020 1434 1682 1717 1020
Size: 414 248 35 3307
Fragments arranged by size:
3307 414 248 35
BanII G_rGCy'C
Cuts at: 199 446 580 1018 2048 199
Size: 247 134 438 1030 2155
Fragments arranged by size:
2155 1030 438 247 134
BbeI G_GCGC'C
Cuts at: 1438 1686 1438
Size: 248 3756
BbsI GAAGACnn'nnnn_
Cuts at: 557 785 557
Size: 228 3776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 226 260 90
Size: 34 102 34 3834
Fragments arranged by size:
3834 102 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1902 2663 4004 1902
Size: 761 1341 1902
Fragments arranged by size:
1902 1341 761
BclI T'GATC_A
Cuts at: 1524 1524
Size: 4004
BglII A'GATC_T
Cuts at: 1519 3556 1519
Size: 2037 1967
BlpI GC'TnA_GC
Cuts at: 1116 1116
Size: 4004
Bme1580I G_kGCm'C
Cuts at: 249 977 1629 1722 2778 249
Size: 728 652 93 1056 1475
Fragments arranged by size:
1475 1056 728 652 93
BmgBI CAC'GTC
Cuts at: 323 972 323
Size: 649 3355
BmrI ACTGGGnnnn_n'
Cuts at: 249 1216 1297 1632 3776 249
Size: 967 81 335 2144 477
Fragments arranged by size:
2144 967 477 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 573 491
Size: 82 3922
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 2059 2551 2849 1180
Size: 879 492 298 2335
Fragments arranged by size:
2335 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3708 3708
Size: 4004
BsaAI yAC'GTr
Cuts at: 1357 1357
Size: 4004
BsaBI GATnn'nnATC
Cuts at: 6 643 1518 3555 6
Size: 637 875 2037 455
Fragments arranged by size:
2037 875 637 455
BsaHI Gr'CG_yC
Cuts at: 1435 1683 2385 3881 1435
Size: 248 702 1496 1558
Fragments arranged by size:
1558 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1193 1714 2392 2666 2813 3224 1193
Size: 521 678 274 147 411 1973
Fragments arranged by size:
1973 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 565 595 712 742 565
Size: 30 117 30 3827
Fragments arranged by size:
3827 117 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 717 717
Size: 4004
BseYI C'CCAG_C
Cuts at: 1429 2187 2764 3260 1429
Size: 758 577 496 2173
Fragments arranged by size:
2173 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 415 438 1546 1592 2800 104
Size: 311 23 1108 46 1208 1308
Fragments arranged by size:
1308 1208 1108 311 46 23
BsiHKAI G_wGCw'C
Cuts at: 199 249 709 977 1018 1796 1986 2778 199
Size: 50 460 268 41 778 190 792 1425
Fragments arranged by size:
1425 792 778 460 268 190 50 41
BsmBI CGTCTCn'nnnn_
Cuts at: 591 591
Size: 4004
Bsp1286I G_dGCh'C
Cuts at: 199 249 446 580 709 977 1018 1629 1722
Size: 50 197 134 129 268 41 611 93
Cuts at: 1722 1796 1986 2048 2778 199
Size: 74 190 62 730 1425
Fragments arranged by size:
1425 730 611 268 197 190 134 129
93 74 62 50 41
BspEI T'CCGG_A
Cuts at: 3224 3224
Size: 4004
BspHI T'CATG_A
Cuts at: 3180 3180
Size: 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 494 1054 1268 1570 1951 494
Size: 560 214 302 381 2547
Fragments arranged by size:
2547 560 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2296 2350 3834 3999 50
Size: 2246 54 1484 165 55
Fragments arranged by size:
2246 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1916 1916
Size: 4004
BsrFI r'CCGG_y
Cuts at: 174 562 2002 2183 2392 174
Size: 388 1440 181 209 1786
Fragments arranged by size:
1786 1440 388 209 181
BsrGI T'GTAC_A
Cuts at: 828 828
Size: 4004
BssHII G'CGCG_C
Cuts at: 260 477 260
Size: 217 3787
BssSI C'ACGA_G
Cuts at: 2275 2633 2275
Size: 358 3646
BstAPI GCAn_nnn'nTGC
Cuts at: 426 1381 426
Size: 955 3049
BstBI TT'CG_AA
Cuts at: 2365 2365
Size: 4004
BstYI r'GATC_y
Cuts at: 1 1029 1496 1519 1854 2100 3112 3198 3210
Size: 1028 467 23 335 246 1012 86 12
Cuts at: 3210 3556 1
Size: 346 449
Fragments arranged by size:
1028 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 165 215 438 165
Size: 50 223 3731
Fragments arranged by size:
3731 223 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1954 2135 2298 1954
Size: 181 163 3660
Fragments arranged by size:
3660 181 163
BtsI GCAGTG_nn'
Cuts at: 573 880 975 1247 573
Size: 307 95 272 3330
Fragments arranged by size:
3330 307 272 95
DraI TTT'AAA
Cuts at: 1003 1003
Size: 4004
DraIII CAC_nnn'GTG
Cuts at: 603 603
Size: 4004
DrdI GACnn_nn'nnGTC
Cuts at: 1710 2568 1710
Size: 858 3146
EaeI y'GGCC_r
Cuts at: 435 1589 1763 2154 2181 3526 435
Size: 1154 174 391 27 1345 913
Fragments arranged by size:
1345 1154 913 391 174 27
EagI C'GGCC_G
Cuts at: 435 1589 435
Size: 1154 2850
EarI CTCTTCn'nnn_
Cuts at: 2027 2237 2027
Size: 210 3794
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 257 2212 2522 2668 3814 257
Size: 1955 310 146 1146 447
Fragments arranged by size:
1955 1146 447 310 146
EcoICRI GAG'CTC
Cuts at: 197 1016 197
Size: 819 3185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 573 817 1829 2261 3008 3276 491
Size: 82 244 1012 432 747 268 1219
Fragments arranged by size:
1219 1012 747 432 268 244 82
EcoNI CCTnn'n_nnAGG
Cuts at: 887 941 887
Size: 54 3950
EcoO109I rG'GnC_Cy
Cuts at: 211 1143 211
Size: 932 3072
EcoRI G'AATT_C
Cuts at: 1008 1008
Size: 4004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 921 2023 921
Size: 1102 2902
FspI TGC'GCA
Cuts at: 327 1785 327
Size: 1458 2546
FspAI rTGC'GCAy
Cuts at: 327 327
Size: 4004
HaeII r_GCGC'y
Cuts at: 369 427 499 563 681 1438 1686 2708 3687
Size: 58 72 64 118 757 248 1022 979
Cuts at: 3687 369
Size: 686
Fragments arranged by size:
1022 979 757 686 248 118 72 64
58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 113 145 565 597 682 714 1846 1876 1878
Size: 32 420 32 85 32 1132 30 2
Cuts at: 1878 1908 113
Size: 30 2209
Fragments arranged by size:
2209 1132 420 85 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 453 690 1043 453
Size: 237 353 3414
Fragments arranged by size:
3414 353 237
KasI G'GCGC_C
Cuts at: 1434 1682 1434
Size: 248 3756
KpnI G_GTAC'C
Cuts at: 1024 1024
Size: 4004
MluI A'CGCG_T
Cuts at: 3437 3437
Size: 4004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 524 2675 2859 524
Size: 2151 184 1669
Fragments arranged by size:
2151 1669 184
MscI TGG'CCA
Cuts at: 1765 1765
Size: 4004
MslI CAynn'nnrTG
Cuts at: 1993 2120 1993
Size: 127 3877
MspA1I CmG'CkG
Cuts at: 114 139 167 417 440 1115 1429 1789 2802
Size: 25 28 250 23 675 314 360 1013
Cuts at: 2802 3047 114
Size: 245 1071
Fragments arranged by size:
1071 1013 675 360 314 250 245 28
25 23
NaeI GCC'GGC
Cuts at: 564 2185 564
Size: 1621 2383
NarI GG'CG_CC
Cuts at: 1435 1683 1435
Size: 248 3756
NgoMIV G'CCGG_C
Cuts at: 562 2183 562
Size: 1621 2383
NspI r_CATG'y
Cuts at: 664 990 1057 1557 2464 664
Size: 326 67 500 907 2204
Fragments arranged by size:
2204 907 500 326 67
PasI CC'CwG_GG
Cuts at: 821 821
Size: 4004
PciI A'CATG_T
Cuts at: 2460 2460
Size: 4004
PflMI CCAn_nnn'nTGG
Cuts at: 131 131
Size: 4004
PmeI GTTT'AAAC
Cuts at: 1003 1003
Size: 4004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 712 744 3143 3175 712
Size: 32 2399 32 1541
Fragments arranged by size:
2399 1541 32 32
PpuMI rG'GwC_Cy
Cuts at: 211 211
Size: 4004
PsiI TTA'TAA
Cuts at: 863 863
Size: 4004
PstI C_TGCA'G
Cuts at: 422 1051 1736 422
Size: 629 685 2690
Fragments arranged by size:
2690 685 629
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4004
PvuII CAG'CTG
Cuts at: 1429 1789 1429
Size: 360 3644
RsrII CG'GwC_CG
Cuts at: 2199 2199
Size: 4004
SacI G_AGCT'C
Cuts at: 199 1018 199
Size: 819 3185
SacII CC_GC'GG
Cuts at: 168 441 168
Size: 273 3731
SalI G'TCGA_C
Cuts at: 1041 1041
Size: 4004
SanDI GG'GwC_CC
Cuts at: 211 211
Size: 4004
SapI GCTCTTCn'nnn_
Cuts at: 2027 2237 2027
Size: 210 3794
SbfI CC_TGCA'GG
Cuts at: 1051 1051
Size: 4004
ScaI AGT'ACT
Cuts at: 3521 3521
Size: 4004
SfcI C'TryA_G
Cuts at: 33 418 981 1047 1732 2725 2916 33
Size: 385 563 66 685 993 191 1121
Fragments arranged by size:
1121 993 685 563 385 191 66
SfoI GGC'GCC
Cuts at: 1436 1684 1436
Size: 248 3756
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
SgrAI Cr'CCGG_yG
Cuts at: 174 174
Size: 4004
SmaI CCC'GGG
Cuts at: 1026 1026
Size: 4004
SmlI C'TyrA_G
Cuts at: 1159 2074 2566 2828 1159
Size: 915 492 262 2335
Fragments arranged by size:
2335 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4004
SphI G_CATG'C
Cuts at: 664 990 1057 1557 664
Size: 326 67 500 3111
Fragments arranged by size:
3111 500 326 67
StyI C'CwwG_G
Cuts at: 956 1138 1492 956
Size: 182 354 3468
Fragments arranged by size:
3468 354 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1587 1720 2179 2388 1587
Size: 133 459 209 3203
Fragments arranged by size:
3203 459 209 133
TatI w'GTAC_w
Cuts at: 828 3519 828
Size: 2691 1313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 3972
Tth111I GACn'n_nGTC
Cuts at: 1801 1801
Size: 4004
XbaI T'CTAG_A
Cuts at: 1035 1035
Size: 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1499 1499
Size: 4004
XmaI C'CCGG_G
Cuts at: 1024 1024
Size: 4004
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI Bpu10I BsgI BsiWI
BsmI BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV
FseI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PshAI PspOMI PspXI
PsrI SexAI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI