Restriction map for
pF1KE2587
(Circular) (Six-base) MAPSORT of: pF1KE2587.seq Check: 229 from: 1 to: 5081
pF1KE2587 5081 bp
With 182 enzymes: SgfI *
August 13, 2015 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1599 1599
Size: 5081
AccI GT'mk_AC
Cuts at: 2119 2119
Size: 5081
Acc65I G'GTAC_C
Cuts at: 2097 2097
Size: 5081
AclI AA'CG_TT
Cuts at: 4921 4921
Size: 5081
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1058 1378 2906 3338 4085 4353 1058
Size: 320 1528 432 747 268 1786
Fragments arranged by size:
1786 1528 747 432 320 268
AflIII A'CryG_T
Cuts at: 2433 3537 4514 2433
Size: 1104 977 3000
Fragments arranged by size:
3000 1104 977
AgeI A'CCGG_T
Cuts at: 3469 3469
Size: 5081
AhdI GACnn_n'nnGTC
Cuts at: 261 261
Size: 5081
AleI CACnn'nnGTG
Cuts at: 505 622 673 505
Size: 117 51 4913
Fragments arranged by size:
4913 117 51
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 446 478 446
Size: 32 5049
AlwNI CAG_nnn'CTG
Cuts at: 259 637 2386 3953 259
Size: 378 1749 1567 1387
Fragments arranged by size:
1749 1567 1387 378
ApaLI G'TGCA_C
Cuts at: 328 3851 328
Size: 3523 1558
ApoI r'AATT_y
Cuts at: 986 1105 1135 1567 1660 2043 2085 4434 4690
Size: 119 30 432 93 383 42 2349 256
Cuts at: 4690 986
Size: 1377
Fragments arranged by size:
2349 1377 432 383 256 119 93 42
30
AseI AT'TA_AT
Cuts at: 20 20
Size: 5081
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5081
AvaI C'yCGr_G
Cuts at: 2101 2101
Size: 5081
BamHI G'GATC_C
Cuts at: 539 2106 539
Size: 1567 3514
BanI G'GyrC_C
Cuts at: 219 412 2097 2511 2759 2794 219
Size: 193 1685 414 248 35 2506
Fragments arranged by size:
2506 1685 414 248 193 35
BanII G_rGCy'C
Cuts at: 1005 2095 3125 1005
Size: 1090 1030 2961
Fragments arranged by size:
2961 1090 1030
BbeI G_GCGC'C
Cuts at: 2515 2763 2515
Size: 248 4833
BbsI GAAGACnn'nnnn_
Cuts at: 179 1246 1585 179
Size: 1067 339 3675
Fragments arranged by size:
3675 1067 339
BbvCI CC'TCA_GC
Cuts at: 1153 1153
Size: 5081
BciVI GTATCCnnnnn_n'
Cuts at: 2979 3740 5081 2979
Size: 761 1341 2979
Fragments arranged by size:
2979 1341 761
BclI T'GATC_A
Cuts at: 641 1031 2601 641
Size: 390 1570 3121
Fragments arranged by size:
3121 1570 390
BfrBI ATG'CAT
Cuts at: 156 757 156
Size: 601 4480
BglI GCCn_nnn'nGGC
Cuts at: 1078 1078
Size: 5081
BglII A'GATC_T
Cuts at: 2596 4633 2596
Size: 2037 3044
BlpI GC'TnA_GC
Cuts at: 814 2193 814
Size: 1379 3702
Bme1580I G_kGCm'C
Cuts at: 222 332 2706 2799 3855 222
Size: 110 2374 93 1056 1448
Fragments arranged by size:
2374 1448 1056 110 93
BmrI ACTGGGnnnn_n'
Cuts at: 159 328 616 668 2293 2374 2709 4853 159
Size: 169 288 52 1625 81 335 2144 387
Fragments arranged by size:
2144 1625 387 335 288 169 81 52
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3448 3448
Size: 5081
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1883 1883
Size: 5081
Bpu10I CC'TnA_GC
Cuts at: 1153 1153
Size: 5081
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1073 2257 3136 3628 3926 1073
Size: 1184 879 492 298 2228
Fragments arranged by size:
2228 1184 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 359 4785 359
Size: 4426 655
BsaAI yAC'GTr
Cuts at: 2434 2434
Size: 5081
BsaBI GATnn'nnATC
Cuts at: 6 1015 2595 4632 6
Size: 1009 1580 2037 455
Fragments arranged by size:
2037 1580 1009 455
BsaHI Gr'CG_yC
Cuts at: 2512 2760 3462 4958 2512
Size: 248 702 1496 2635
Fragments arranged by size:
2635 1496 702 248
BsaWI w'CCGG_w
Cuts at: 727 2270 2791 3469 3743 3890 4301 727
Size: 1543 521 678 274 147 411 1507
Fragments arranged by size:
1543 1507 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 510 2506 3264 3841 4337 510
Size: 1996 758 577 496 1254
Fragments arranged by size:
1996 1254 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2623 2669 3877 104
Size: 2519 46 1208 1308
Fragments arranged by size:
2519 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 332 1005 2095 2873 3063 3855 332
Size: 673 1090 778 190 792 1558
Fragments arranged by size:
1558 1090 792 778 673 190
BsmI GAATG_Cn'
Cuts at: 1370 1656 1370
Size: 286 4795
Bsp1286I G_dGCh'C
Cuts at: 222 332 1005 2095 2706 2799 2873 3063 3125
Size: 110 673 1090 611 93 74 190 62
Cuts at: 3125 3855 222
Size: 730 1448
Fragments arranged by size:
1448 1090 730 673 611 190 110 93
74 62
BspEI T'CCGG_A
Cuts at: 727 4301 727
Size: 3574 1507
BspHI T'CATG_A
Cuts at: 4257 4257
Size: 5081
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1599 2131 2345 2647 3028 1599
Size: 532 214 302 381 3652
Fragments arranged by size:
3652 532 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3373 3427 4911 5076 50
Size: 3323 54 1484 165 55
Fragments arranged by size:
3323 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 656 796 1538 1747 1785 1961 2993 656
Size: 140 742 209 38 176 1032 2744
Fragments arranged by size:
2744 1032 742 209 176 140 38
BsrFI r'CCGG_y
Cuts at: 3079 3260 3469 3079
Size: 181 209 4691
Fragments arranged by size:
4691 209 181
BsrGI T'GTAC_A
Cuts at: 1223 1933 1223
Size: 710 4371
BssSI C'ACGA_G
Cuts at: 3352 3710 3352
Size: 358 4723
BstAPI GCAn_nnn'nTGC
Cuts at: 919 2458 919
Size: 1539 3542
BstBI TT'CG_AA
Cuts at: 3442 3442
Size: 5081
BstEII G'GTnAC_C
Cuts at: 291 291
Size: 5081
BstXI CCAn_nnnn'nTGG
Cuts at: 450 624 675 450
Size: 174 51 4856
Fragments arranged by size:
4856 174 51
BstYI r'GATC_y
Cuts at: 1 539 1093 2106 2573 2596 2931 3177 4189
Size: 538 554 1013 467 23 335 246 1012
Cuts at: 4189 4275 4287 4633 1
Size: 86 12 346 449
Fragments arranged by size:
1013 1012 554 538 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 449 1146 1202 449
Size: 697 56 4328
Fragments arranged by size:
4328 697 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3031 3212 3375 3031
Size: 181 163 4737
Fragments arranged by size:
4737 181 163
BtsI GCAGTG_nn'
Cuts at: 618 1842 2324 618
Size: 1224 482 3375
Fragments arranged by size:
3375 1224 482
ClaI AT'CG_AT
Cuts at: 1415 1415
Size: 5081
DraI TTT'AAA
Cuts at: 861 1388 1572 2080 861
Size: 527 184 508 3862
Fragments arranged by size:
3862 527 508 184
DraIII CAC_nnn'GTG
Cuts at: 336 336
Size: 5081
DrdI GACnn_nn'nnGTC
Cuts at: 2787 3645 2787
Size: 858 4223
EaeI y'GGCC_r
Cuts at: 587 2666 2840 3231 3258 4603 587
Size: 2079 174 391 27 1345 1065
Fragments arranged by size:
2079 1345 1065 391 174 27
EagI C'GGCC_G
Cuts at: 2666 2666
Size: 5081
EarI CTCTTCn'nnn_
Cuts at: 3104 3314 3104
Size: 210 4871
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3289 3599 3745 4891 3289
Size: 310 146 1146 3479
Fragments arranged by size:
3479 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1003 2093 1003
Size: 1090 3991
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1058 1378 1883 2906 3338 4085 4353 1058
Size: 320 505 1023 432 747 268 1786
Fragments arranged by size:
1786 1023 747 505 432 320 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1606 1606
Size: 5081
EcoO109I rG'GnC_Cy
Cuts at: 1300 2220 1300
Size: 920 4161
EcoRI G'AATT_C
Cuts at: 986 2085 986
Size: 1099 3982
EcoRV GAT'ATC
Cuts at: 1725 1725
Size: 5081
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 817 898 3100 817
Size: 81 2202 2798
Fragments arranged by size:
2798 2202 81
FspI TGC'GCA
Cuts at: 2862 2862
Size: 5081
HaeII r_GCGC'y
Cuts at: 2515 2763 3785 4764 2515
Size: 248 1022 979 2832
Fragments arranged by size:
2832 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 247 279 450 482 2923 2953 2955 2985 247
Size: 32 171 32 2441 30 2 30 2343
Fragments arranged by size:
2441 2343 171 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2120 2120
Size: 5081
HindIII A'AGCT_T
Cuts at: 1040 1787 1040
Size: 747 4334
KasI G'GCGC_C
Cuts at: 2511 2759 2511
Size: 248 4833
KpnI G_GTAC'C
Cuts at: 2101 2101
Size: 5081
MfeI C'AATT_G
Cuts at: 1118 1118
Size: 5081
MluI A'CGCG_T
Cuts at: 4514 4514
Size: 5081
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 545 3752 3936 545
Size: 3207 184 1690
Fragments arranged by size:
3207 1690 184
MscI TGG'CCA
Cuts at: 589 2842 589
Size: 2253 2828
MslI CAynn'nnrTG
Cuts at: 505 595 622 673 937 3070 3197 505
Size: 90 27 51 264 2133 127 2389
Fragments arranged by size:
2389 2133 264 127 90 51 27
MspA1I CmG'CkG
Cuts at: 2192 2506 2866 3879 4124 2192
Size: 314 360 1013 245 3149
Fragments arranged by size:
3149 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3262 3262
Size: 5081
NarI GG'CG_CC
Cuts at: 2512 2760 2512
Size: 248 4833
NcoI C'CATG_G
Cuts at: 1146 1202 1146
Size: 56 5025
NdeI CA'TA_TG
Cuts at: 759 951 1440 759
Size: 192 489 4400
Fragments arranged by size:
4400 489 192
NgoMIV G'CCGG_C
Cuts at: 3260 3260
Size: 5081
NsiI A_TGCA'T
Cuts at: 158 759 158
Size: 601 4480
NspI r_CATG'y
Cuts at: 141 477 2134 2634 3541 141
Size: 336 1657 500 907 1681
Fragments arranged by size:
1681 1657 907 500 336
PciI A'CATG_T
Cuts at: 3537 3537
Size: 5081
PflMI CCAn_nnn'nTGG
Cuts at: 303 340 303
Size: 37 5044
PfoI T'CCnGG_A
Cuts at: 1826 1826
Size: 5081
PmeI GTTT'AAAC
Cuts at: 2080 2080
Size: 5081
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1006 1038 4220 4252 1006
Size: 32 3182 32 1835
Fragments arranged by size:
3182 1835 32 32
PpuMI rG'GwC_Cy
Cuts at: 1300 1300
Size: 5081
PstI C_TGCA'G
Cuts at: 555 1607 2128 2813 555
Size: 1052 521 685 2823
Fragments arranged by size:
2823 1052 685 521
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5081
PvuII CAG'CTG
Cuts at: 2506 2866 2506
Size: 360 4721
RsrII CG'GwC_CG
Cuts at: 3276 3276
Size: 5081
SacI G_AGCT'C
Cuts at: 1005 2095 1005
Size: 1090 3991
SalI G'TCGA_C
Cuts at: 2118 2118
Size: 5081
SapI GCTCTTCn'nnn_
Cuts at: 3104 3314 3104
Size: 210 4871
SbfI CC_TGCA'GG
Cuts at: 2128 2128
Size: 5081
ScaI AGT'ACT
Cuts at: 4598 4598
Size: 5081
SfcI C'TryA_G
Cuts at: 33 551 887 1505 1603 2124 2809 3802 3993
Size: 518 336 618 98 521 685 993 191
Cuts at: 3993 33
Size: 1121
Fragments arranged by size:
1121 993 685 618 521 518 336 191
98
SfoI GGC'GCC
Cuts at: 2513 2761 2513
Size: 248 4833
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5081
SmaI CCC'GGG
Cuts at: 2103 2103
Size: 5081
SmlI C'TyrA_G
Cuts at: 1088 2236 3151 3643 3905 1088
Size: 1148 915 492 262 2264
Fragments arranged by size:
2264 1148 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5081
SphI G_CATG'C
Cuts at: 141 2134 2634 141
Size: 1993 500 2588
Fragments arranged by size:
2588 1993 500
SspI AAT'ATT
Cuts at: 1114 1396 1114
Size: 282 4799
StyI C'CwwG_G
Cuts at: 297 1146 1202 2215 2569 297
Size: 849 56 1013 354 2809
Fragments arranged by size:
2809 1013 849 354 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 347 685 2664 2797 3256 3465 347
Size: 338 1979 133 459 209 1963
Fragments arranged by size:
1979 1963 459 338 209 133
TatI w'GTAC_w
Cuts at: 1223 1435 1933 4596 1223
Size: 212 498 2663 1708
Fragments arranged by size:
2663 1708 498 212
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4598 4630 4598
Size: 32 5049
Tth111I GACn'n_nGTC
Cuts at: 2878 2878
Size: 5081
XbaI T'CTAG_A
Cuts at: 2112 2112
Size: 5081
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 387 2576 387
Size: 2189 2892
XmaI C'CCGG_G
Cuts at: 2101 2101
Size: 5081
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI ApaI AscI AvrII BaeI
BcgI BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmBI
BssHII BstZ17I Bsu36I CspCI FseI FspAI HpaI NheI
NotI NruI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI