Restriction map for
pF1KE2575
(Circular) (Six-base) MAPSORT of: pF1KE2575.seq Check: 2728 from: 1 to: 3638
pF1KE2575 3638 bp
With 182 enzymes: SgfI *
May 26, 2015 10:14 ..
AccI GT'mk_AC
Cuts at: 676 676
Size: 3638
Acc65I G'GTAC_C
Cuts at: 654 654
Size: 3638
AclI AA'CG_TT
Cuts at: 3478 3478
Size: 3638
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 1463 1895 2642 2910 213
Size: 1250 432 747 268 941
Fragments arranged by size:
1250 941 747 432 268
AflIII A'CryG_T
Cuts at: 990 2094 3071 990
Size: 1104 977 1557
Fragments arranged by size:
1557 1104 977
AgeI A'CCGG_T
Cuts at: 2026 2026
Size: 3638
AhdI GACnn_n'nnGTC
Cuts at: 588 588
Size: 3638
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 529 561 529
Size: 32 3606
AlwNI CAG_nnn'CTG
Cuts at: 943 2510 943
Size: 1567 2071
ApaI G_GGCC'C
Cuts at: 358 358
Size: 3638
ApaLI G'TGCA_C
Cuts at: 2408 2408
Size: 3638
ApoI r'AATT_y
Cuts at: 642 2991 3247 642
Size: 2349 256 1033
Fragments arranged by size:
2349 1033 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3638
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
AvaI C'yCGr_G
Cuts at: 131 415 658 131
Size: 284 243 3111
Fragments arranged by size:
3111 284 243
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 199 232 199
Size: 33 3605
BamHI G'GATC_C
Cuts at: 663 663
Size: 3638
BanI G'GyrC_C
Cuts at: 654 1068 1316 1351 654
Size: 414 248 35 2941
Fragments arranged by size:
2941 414 248 35
BanII G_rGCy'C
Cuts at: 358 652 1682 358
Size: 294 1030 2314
Fragments arranged by size:
2314 1030 294
BbeI G_GCGC'C
Cuts at: 1072 1320 1072
Size: 248 3390
BbsI GAAGACnn'nnnn_
Cuts at: 366 366
Size: 3638
BbvCI CC'TCA_GC
Cuts at: 530 530
Size: 3638
BciVI GTATCCnnnnn_n'
Cuts at: 1536 2297 3638 1536
Size: 761 1341 1536
Fragments arranged by size:
1536 1341 761
BclI T'GATC_A
Cuts at: 1158 1158
Size: 3638
BglII A'GATC_T
Cuts at: 1153 3190 1153
Size: 2037 1601
BlpI GC'TnA_GC
Cuts at: 750 750
Size: 3638
Bme1580I G_kGCm'C
Cuts at: 358 1263 1356 2412 358
Size: 905 93 1056 1584
Fragments arranged by size:
1584 1056 905 93
BmrI ACTGGGnnnn_n'
Cuts at: 850 931 1266 3410 850
Size: 81 335 2144 1078
Fragments arranged by size:
2144 1078 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 306 2005 306
Size: 1699 1939
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 509
Size: 3638
Bpu10I CC'TnA_GC
Cuts at: 530 530
Size: 3638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1693 2185 2483 814
Size: 879 492 298 1969
Fragments arranged by size:
1969 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 277 3342 277
Size: 3065 573
BsaAI yAC'GTr
Cuts at: 991 991
Size: 3638
BsaBI GATnn'nnATC
Cuts at: 6 1152 3189 6
Size: 1146 2037 455
Fragments arranged by size:
2037 1146 455
BsaHI Gr'CG_yC
Cuts at: 332 1069 1317 2019 3515 332
Size: 737 248 702 1496 455
Fragments arranged by size:
1496 737 702 455 248
BsaWI w'CCGG_w
Cuts at: 827 1348 2026 2300 2447 2858 827
Size: 521 678 274 147 411 1607
Fragments arranged by size:
1607 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 529 559 529
Size: 30 3608
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 334 337 334
Size: 3 3635
BseYI C'CCAG_C
Cuts at: 293 297 454 1063 1821 2398 2894 293
Size: 4 157 609 758 577 496 1037
Fragments arranged by size:
1037 758 609 577 496 157 4
BsiEI CG_ry'CG
Cuts at: 104 1180 1226 2434 104
Size: 1076 46 1208 1308
Fragments arranged by size:
1308 1208 1076 46
BsiHKAI G_wGCw'C
Cuts at: 652 1430 1620 2412 652
Size: 778 190 792 1878
Fragments arranged by size:
1878 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 611 611
Size: 3638
Bsp1286I G_dGCh'C
Cuts at: 358 652 1263 1356 1430 1620 1682 2412 358
Size: 294 611 93 74 190 62 730 1584
Fragments arranged by size:
1584 730 611 294 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2858 2858
Size: 3638
BspHI T'CATG_A
Cuts at: 2814 2814
Size: 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 688 902 1204 1585 688
Size: 214 302 381 2741
Fragments arranged by size:
2741 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1930 1984 3468 3633 50
Size: 1880 54 1484 165 55
Fragments arranged by size:
1880 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1550 1550
Size: 3638
BsrFI r'CCGG_y
Cuts at: 1636 1817 2026 1636
Size: 181 209 3248
Fragments arranged by size:
3248 209 181
BssSI C'ACGA_G
Cuts at: 1909 2267 1909
Size: 358 3280
BstAPI GCAn_nnn'nTGC
Cuts at: 263 1015 263
Size: 752 2886
BstBI TT'CG_AA
Cuts at: 1999 1999
Size: 3638
BstXI CCAn_nnnn'nTGG
Cuts at: 567 567
Size: 3638
BstYI r'GATC_y
Cuts at: 1 663 1130 1153 1488 1734 2746 2832 2844
Size: 662 467 23 335 246 1012 86 12
Cuts at: 2844 3190 1
Size: 346 449
Fragments arranged by size:
1012 662 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 277 277
Size: 3638
BtgI C'CryG_G
Cuts at: 107 560 612 107
Size: 453 52 3133
Fragments arranged by size:
3133 453 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1588 1769 1932 1588
Size: 181 163 3294
Fragments arranged by size:
3294 181 163
BtsI GCAGTG_nn'
Cuts at: 881 881
Size: 3638
DraI TTT'AAA
Cuts at: 637 637
Size: 3638
DrdI GACnn_nn'nnGTC
Cuts at: 1344 2202 1344
Size: 858 2780
EaeI y'GGCC_r
Cuts at: 110 1223 1397 1788 1815 3160 110
Size: 1113 174 391 27 1345 588
Fragments arranged by size:
1345 1113 588 391 174 27
EagI C'GGCC_G
Cuts at: 1223 1223
Size: 3638
EarI CTCTTCn'nnn_
Cuts at: 1661 1871 1661
Size: 210 3428
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1846 2156 2302 3448 1846
Size: 310 146 1146 2036
Fragments arranged by size:
2036 1146 310 146
EcoICRI GAG'CTC
Cuts at: 650 650
Size: 3638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 509 1463 1895 2642 2910 213
Size: 296 954 432 747 268 941
Fragments arranged by size:
954 941 747 432 296 268
EcoNI CCTnn'n_nnAGG
Cuts at: 505 505
Size: 3638
EcoO109I rG'GnC_Cy
Cuts at: 128 354 355 777 128
Size: 226 1 422 2989
Fragments arranged by size:
2989 422 226 1
EcoRI G'AATT_C
Cuts at: 642 642
Size: 3638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1657 1657
Size: 3638
FspI TGC'GCA
Cuts at: 1419 1419
Size: 3638
HaeII r_GCGC'y
Cuts at: 1072 1320 2342 3321 1072
Size: 248 1022 979 1389
Fragments arranged by size:
1389 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 505 537 574 606 1480 1510 1512 1542 505
Size: 32 37 32 874 30 2 30 2601
Fragments arranged by size:
2601 874 37 32 32 30 30 2
HincII GTy'rAC
Cuts at: 677 677
Size: 3638
KasI G'GCGC_C
Cuts at: 1068 1316 1068
Size: 248 3390
KpnI G_GTAC'C
Cuts at: 658 658
Size: 3638
MluI A'CGCG_T
Cuts at: 3071 3071
Size: 3638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 200 2309 2493 200
Size: 2109 184 1345
Fragments arranged by size:
2109 1345 184
MscI TGG'CCA
Cuts at: 112 1399 112
Size: 1287 2351
MslI CAynn'nnrTG
Cuts at: 1627 1754 1627
Size: 127 3511
MspA1I CmG'CkG
Cuts at: 233 297 458 529 749 1063 1423 2436 2681
Size: 64 161 71 220 314 360 1013 245
Cuts at: 2681 233
Size: 1190
Fragments arranged by size:
1190 1013 360 314 245 220 161 71
64
NaeI GCC'GGC
Cuts at: 1819 1819
Size: 3638
NarI GG'CG_CC
Cuts at: 1069 1317 1069
Size: 248 3390
NcoI C'CATG_G
Cuts at: 107 560 612 107
Size: 453 52 3133
Fragments arranged by size:
3133 453 52
NgoMIV G'CCGG_C
Cuts at: 1817 1817
Size: 3638
NspI r_CATG'y
Cuts at: 447 691 1191 2098 447
Size: 244 500 907 1987
Fragments arranged by size:
1987 907 500 244
PciI A'CATG_T
Cuts at: 2094 2094
Size: 3638
PmeI GTTT'AAAC
Cuts at: 637 637
Size: 3638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 529 561 2777 2809 529
Size: 32 2216 32 1358
Fragments arranged by size:
2216 1358 32 32
PpuMI rG'GwC_Cy
Cuts at: 128 128
Size: 3638
PspOMI G'GGCC_C
Cuts at: 354 354
Size: 3638
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 568 600 568
Size: 32 3606
PstI C_TGCA'G
Cuts at: 685 1370 685
Size: 685 2953
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3638
PvuII CAG'CTG
Cuts at: 233 297 529 1063 1423 233
Size: 64 232 534 360 2448
Fragments arranged by size:
2448 534 360 232 64
RsrII CG'GwC_CG
Cuts at: 1833 1833
Size: 3638
SacI G_AGCT'C
Cuts at: 652 652
Size: 3638
SalI G'TCGA_C
Cuts at: 675 675
Size: 3638
SanDI GG'GwC_CC
Cuts at: 128 128
Size: 3638
SapI GCTCTTCn'nnn_
Cuts at: 1661 1871 1661
Size: 210 3428
SbfI CC_TGCA'GG
Cuts at: 685 685
Size: 3638
ScaI AGT'ACT
Cuts at: 3155 3155
Size: 3638
SfcI C'TryA_G
Cuts at: 33 681 1366 2359 2550 33
Size: 648 685 993 191 1121
Fragments arranged by size:
1121 993 685 648 191
SfoI GGC'GCC
Cuts at: 1070 1318 1070
Size: 248 3390
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
SmaI CCC'GGG
Cuts at: 133 660 133
Size: 527 3111
SmlI C'TyrA_G
Cuts at: 793 1708 2200 2462 793
Size: 915 492 262 1969
Fragments arranged by size:
1969 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3638
SphI G_CATG'C
Cuts at: 691 1191 691
Size: 500 3138
StyI C'CwwG_G
Cuts at: 107 560 612 772 1126 107
Size: 453 52 160 354 2619
Fragments arranged by size:
2619 453 354 160 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 573 625 1221 1354 1813 2022 573
Size: 52 596 133 459 209 2189
Fragments arranged by size:
2189 596 459 209 133 52
TatI w'GTAC_w
Cuts at: 3153 3153
Size: 3638
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 294 326 3155 3187 294
Size: 32 2829 32 745
Fragments arranged by size:
2829 745 32 32
Tth111I GACn'n_nGTC
Cuts at: 1435 1435
Size: 3638
XbaI T'CTAG_A
Cuts at: 669 669
Size: 3638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 250 1133 250
Size: 883 2755
XmaI C'CCGG_G
Cuts at: 131 658 131
Size: 527 3111
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AscI AvrII
BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspXI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI