Restriction map for
pF1KE2551
(Circular) (Six-base) MAPSORT of: pF1KE2551.seq Check: 1518 from: 1 to: 5003
pF1KE2551 5003 bp
With 182 enzymes: SgfI *
December 2, 2014 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1531 1531
Size: 5003
AccI GT'mk_AC
Cuts at: 2041 2041
Size: 5003
Acc65I G'GTAC_C
Cuts at: 2019 2019
Size: 5003
AclI AA'CG_TT
Cuts at: 4843 4843
Size: 5003
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 894 1683 2828 3260 4007 4275 812
Size: 82 789 1145 432 747 268 1540
Fragments arranged by size:
1540 1145 789 747 432 268 82
AflIII A'CryG_T
Cuts at: 1473 2355 3459 4436 1473
Size: 882 1104 977 2040
Fragments arranged by size:
2040 1104 977 882
AgeI A'CCGG_T
Cuts at: 827 3391 827
Size: 2564 2439
AleI CACnn'nnGTG
Cuts at: 316 1453 316
Size: 1137 3866
AlwNI CAG_nnn'CTG
Cuts at: 849 917 1639 2308 3875 849
Size: 68 722 669 1567 1977
Fragments arranged by size:
1977 1567 722 669 68
ApaI G_GGCC'C
Cuts at: 1604 1604
Size: 5003
ApaLI G'TGCA_C
Cuts at: 3773 3773
Size: 5003
ApoI r'AATT_y
Cuts at: 126 796 1964 2007 4356 4612 126
Size: 670 1168 43 2349 256 517
Fragments arranged by size:
2349 1168 670 517 256 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 5003
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5003
AvaI C'yCGr_G
Cuts at: 1704 2023 1704
Size: 319 4684
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1812 1845 1812
Size: 33 4970
BamHI G'GATC_C
Cuts at: 1534 2028 1534
Size: 494 4509
BanI G'GyrC_C
Cuts at: 1003 2019 2433 2681 2716 1003
Size: 1016 414 248 35 3290
Fragments arranged by size:
3290 1016 414 248 35
BanII G_rGCy'C
Cuts at: 518 842 1604 1786 2017 3047 518
Size: 324 762 182 231 1030 2474
Fragments arranged by size:
2474 1030 762 324 231 182
BbeI G_GCGC'C
Cuts at: 2437 2685 2437
Size: 248 4755
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 527 561 527
Size: 34 4969
BciVI GTATCCnnnnn_n'
Cuts at: 2901 3662 5003 2901
Size: 761 1341 2901
Fragments arranged by size:
2901 1341 761
BclI T'GATC_A
Cuts at: 1445 1898 2523 1445
Size: 453 625 3925
Fragments arranged by size:
3925 625 453
BfrBI ATG'CAT
Cuts at: 548 548
Size: 5003
BglII A'GATC_T
Cuts at: 1103 2518 4555 1103
Size: 1415 2037 1551
Fragments arranged by size:
2037 1551 1415
BlpI GC'TnA_GC
Cuts at: 323 2115 323
Size: 1792 3211
Bme1580I G_kGCm'C
Cuts at: 1604 2628 2721 3777 1604
Size: 1024 93 1056 2830
Fragments arranged by size:
2830 1056 1024 93
BmgBI CAC'GTC
Cuts at: 782 813 1476 1680 782
Size: 31 663 204 4105
Fragments arranged by size:
4105 663 204 31
BmrI ACTGGGnnnn_n'
Cuts at: 532 2215 2296 2631 4775 532
Size: 1683 81 335 2144 760
Fragments arranged by size:
2144 1683 760 335 81
BmtI G_CTAG'C
Cuts at: 1154 1154
Size: 5003
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3370 3370
Size: 5003
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1037 1044 1090 1603 1737 1845 1892 826
Size: 211 7 46 513 134 108 47 3937
Fragments arranged by size:
3937 513 211 134 108 47 46 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2179 3058 3550 3848 2179
Size: 879 492 298 3334
Fragments arranged by size:
3334 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 340 4707 340
Size: 4367 636
BsaAI yAC'GTr
Cuts at: 1334 1569 2356 1334
Size: 235 787 3981
Fragments arranged by size:
3981 787 235
BsaBI GATnn'nnATC
Cuts at: 6 1399 2517 4554 6
Size: 1393 1118 2037 455
Fragments arranged by size:
2037 1393 1118 455
BsaHI Gr'CG_yC
Cuts at: 118 926 2434 2682 3384 4880 118
Size: 808 1508 248 702 1496 241
Fragments arranged by size:
1508 1496 808 702 248 241
BsaWI w'CCGG_w
Cuts at: 827 2192 2713 3391 3665 3812 4223 827
Size: 1365 521 678 274 147 411 1607
Fragments arranged by size:
1607 1365 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 655 685 655
Size: 30 4973
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 663 1312 1600 451
Size: 212 649 288 3854
Fragments arranged by size:
3854 649 288 212
BseYI C'CCAG_C
Cuts at: 297 1315 1407 1727 2428 3186 3763 4259 297
Size: 1018 92 320 701 758 577 496 1041
Fragments arranged by size:
1041 1018 758 701 577 496 320 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 717 717
Size: 5003
BsiEI CG_ry'CG
Cuts at: 104 2545 2591 3799 104
Size: 2441 46 1208 1308
Fragments arranged by size:
2441 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 518 1256 1508 1613 2017 2795 2985 3777 518
Size: 738 252 105 404 778 190 792 1744
Fragments arranged by size:
1744 792 778 738 404 252 190 105
BsmI GAATG_Cn'
Cuts at: 454 1995 454
Size: 1541 3462
BsmBI CGTCTCn'nnnn_
Cuts at: 106 106
Size: 5003
Bsp1286I G_dGCh'C
Cuts at: 518 842 1256 1508 1604 1613 1786 2017 2628
Size: 324 414 252 96 9 173 231 611
Cuts at: 2628 2721 2795 2985 3047 3777 518
Size: 93 74 190 62 730 1744
Fragments arranged by size:
1744 730 611 414 324 252 231 190
173 96 93 74 62 9
BspEI T'CCGG_A
Cuts at: 4223 4223
Size: 5003
BspHI T'CATG_A
Cuts at: 4179 4179
Size: 5003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1300 1531 2053 2267 2569 2950 1300
Size: 231 522 214 302 381 3353
Fragments arranged by size:
3353 522 381 302 231 214
BsrBI CCG'CTC
Cuts at: 50 501 1099 1497 3295 3349 4833 4998 50
Size: 451 598 398 1798 54 1484 165 55
Fragments arranged by size:
1798 1484 598 451 398 165 55 54
BsrDI GCAATG_nn'
Cuts at: 414 608 620 2915 414
Size: 194 12 2295 2502
Fragments arranged by size:
2502 2295 194 12
BsrFI r'CCGG_y
Cuts at: 827 3001 3182 3391 827
Size: 2174 181 209 2439
Fragments arranged by size:
2439 2174 209 181
BssSI C'ACGA_G
Cuts at: 3274 3632 3274
Size: 358 4645
BstAPI GCAn_nnn'nTGC
Cuts at: 2380 2380
Size: 5003
BstBI TT'CG_AA
Cuts at: 3364 3364
Size: 5003
BstEII G'GTnAC_C
Cuts at: 1194 1542 1194
Size: 348 4655
BstXI CCAn_nnnn'nTGG
Cuts at: 318 541 1478 318
Size: 223 937 3843
Fragments arranged by size:
3843 937 223
BstYI r'GATC_y
Cuts at: 1 821 1103 1534 1721 2028 2495 2518 2853
Size: 820 282 431 187 307 467 23 335
Cuts at: 2853 3099 4111 4197 4209 4555 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 820 467 449 431 346 335 307
282 246 187 86 23 12
BtgI C'CryG_G
Cuts at: 107 1511 1595 107
Size: 1404 84 3515
Fragments arranged by size:
3515 1404 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2953 3134 3297 2953
Size: 181 163 4659
Fragments arranged by size:
4659 181 163
BtsI GCAGTG_nn'
Cuts at: 1613 2246 1613
Size: 633 4370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1356 1391 1356
Size: 35 4968
DraI TTT'AAA
Cuts at: 1920 2002 1920
Size: 82 4921
DrdI GACnn_nn'nnGTC
Cuts at: 2709 3567 2709
Size: 858 4145
EaeI y'GGCC_r
Cuts at: 479 1298 1733 2588 2762 3153 3180 4525 479
Size: 819 435 855 174 391 27 1345 957
Fragments arranged by size:
1345 957 855 819 435 391 174 27
EagI C'GGCC_G
Cuts at: 2588 2588
Size: 5003
EarI CTCTTCn'nnn_
Cuts at: 1042 1330 3026 3236 1042
Size: 288 1696 210 2809
Fragments arranged by size:
2809 1696 288 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3211 3521 3667 4813 3211
Size: 310 146 1146 3401
Fragments arranged by size:
3401 1146 310 146
EcoICRI GAG'CTC
Cuts at: 516 2015 516
Size: 1499 3504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 826 894 1037 1044 1090 1603 1683 1737
Size: 14 68 143 7 46 513 80 54
Cuts at: 1737 1845 1892 2828 3260 4007 4275 812
Size: 108 47 936 432 747 268 1540
Fragments arranged by size:
1540 936 747 513 432 268 143 108
80 68 54 47 46 14 7
EcoO109I rG'GnC_Cy
Cuts at: 846 1134 1312 1562 1600 2142 846
Size: 288 178 250 38 542 3707
Fragments arranged by size:
3707 542 288 250 178 38
EcoRI G'AATT_C
Cuts at: 126 2007 126
Size: 1881 3122
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 5003
FspI TGC'GCA
Cuts at: 2784 2784
Size: 5003
HaeII r_GCGC'y
Cuts at: 420 658 1491 2437 2685 3707 4686 420
Size: 238 833 946 248 1022 979 737
Fragments arranged by size:
1022 979 946 833 737 248 238
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1226 1258 1526 1558 1890 1922 2845 2875 2877
Size: 32 268 32 332 32 923 30 2
Cuts at: 2877 2907 1226
Size: 30 3322
Fragments arranged by size:
3322 923 332 268 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2042 2042
Size: 5003
KasI G'GCGC_C
Cuts at: 2433 2681 2433
Size: 248 4755
KpnI G_GTAC'C
Cuts at: 2023 2023
Size: 5003
MfeI C'AATT_G
Cuts at: 1053 1053
Size: 5003
MluI A'CGCG_T
Cuts at: 4436 4436
Size: 5003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3674 3858 3674
Size: 184 4819
MscI TGG'CCA
Cuts at: 481 1300 1735 2764 481
Size: 819 435 1029 2720
Fragments arranged by size:
2720 1029 819 435
MslI CAynn'nnrTG
Cuts at: 316 475 539 643 1453 2992 3119 316
Size: 159 64 104 810 1539 127 2200
Fragments arranged by size:
2200 1539 810 159 127 104 64
MspA1I CmG'CkG
Cuts at: 690 704 863 870 2114 2428 2788 3801 4046
Size: 14 159 7 1244 314 360 1013 245
Cuts at: 4046 690
Size: 1647
Fragments arranged by size:
1647 1244 1013 360 314 245 159 14
7
NaeI GCC'GGC
Cuts at: 3184 3184
Size: 5003
NarI GG'CG_CC
Cuts at: 2434 2682 2434
Size: 248 4755
NcoI C'CATG_G
Cuts at: 107 107
Size: 5003
NdeI CA'TA_TG
Cuts at: 430 430
Size: 5003
NgoMIV G'CCGG_C
Cuts at: 3182 3182
Size: 5003
NheI G'CTAG_C
Cuts at: 1150 1150
Size: 5003
NsiI A_TGCA'T
Cuts at: 550 550
Size: 5003
NspI r_CATG'y
Cuts at: 1359 2056 2556 3463 1359
Size: 697 500 907 2899
Fragments arranged by size:
2899 907 697 500
PasI CC'CwG_GG
Cuts at: 208 208
Size: 5003
PciI A'CATG_T
Cuts at: 3459 3459
Size: 5003
PflMI CCAn_nnn'nTGG
Cuts at: 265 849 265
Size: 584 4419
PfoI T'CCnGG_A
Cuts at: 715 842 1067 715
Size: 127 225 4651
Fragments arranged by size:
4651 225 127
PmeI GTTT'AAAC
Cuts at: 2002 2002
Size: 5003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 4142
Size: 32 4971
PpuMI rG'GwC_Cy
Cuts at: 846 1134 1312 846
Size: 288 178 4537
Fragments arranged by size:
4537 288 178
PshAI GACnn'nnGTC
Cuts at: 1310 1310
Size: 5003
PspOMI G'GGCC_C
Cuts at: 1600 1600
Size: 5003
PstI C_TGCA'G
Cuts at: 821 1675 2050 2735 821
Size: 854 375 685 3089
Fragments arranged by size:
3089 854 685 375
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5003
PvuII CAG'CTG
Cuts at: 690 704 863 870 2428 2788 690
Size: 14 159 7 1558 360 2905
Fragments arranged by size:
2905 1558 360 159 14 7
RsrII CG'GwC_CG
Cuts at: 3198 3198
Size: 5003
SacI G_AGCT'C
Cuts at: 518 2017 518
Size: 1499 3504
SalI G'TCGA_C
Cuts at: 2040 2040
Size: 5003
SapI GCTCTTCn'nnn_
Cuts at: 3026 3236 3026
Size: 210 4793
SbfI CC_TGCA'GG
Cuts at: 2050 2050
Size: 5003
ScaI AGT'ACT
Cuts at: 4520 4520
Size: 5003
SfcI C'TryA_G
Cuts at: 33 187 817 1671 2046 2731 3724 3915 33
Size: 154 630 854 375 685 993 191 1121
Fragments arranged by size:
1121 993 854 685 630 375 191 154
SfoI GGC'GCC
Cuts at: 2435 2683 2435
Size: 248 4755
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5003
SmaI CCC'GGG
Cuts at: 1706 2025 1706
Size: 319 4684
SmlI C'TyrA_G
Cuts at: 2158 3073 3565 3827 2158
Size: 915 492 262 3334
Fragments arranged by size:
3334 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5003
SphI G_CATG'C
Cuts at: 2056 2556 2056
Size: 500 4503
StyI C'CwwG_G
Cuts at: 107 2137 2491 107
Size: 2030 354 2619
Fragments arranged by size:
2619 2030 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 942 1719 2586 2719 3178 3387 942
Size: 777 867 133 459 209 2558
Fragments arranged by size:
2558 867 777 459 209 133
TatI w'GTAC_w
Cuts at: 4518 4518
Size: 5003
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4520 4552 4520
Size: 32 4971
Tth111I GACn'n_nGTC
Cuts at: 2800 2800
Size: 5003
XbaI T'CTAG_A
Cuts at: 2034 2034
Size: 5003
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 315 2498 315
Size: 2183 2820
XmaI C'CCGG_G
Cuts at: 1704 2023 1704
Size: 319 4684
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BbsI BbvCI BglI Bpu10I BsiWI BsrGI BssHII BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI NotI NruI PacI PmlI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI