Restriction map for
pF1KE2549
(Circular) (Six-base) MAPSORT of: pF1KE2549.seq Check: 9126 from: 1 to: 7622
pF1KE2549 7622 bp
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2263 3258 3383 3645 4524 2263
Size: 995 125 262 879 5361
Fragments arranged by size:
5361 995 879 262 125
AatII G_ACGT'C
Cuts at: 605 1193 2726 2909 3734 605
Size: 588 1533 183 825 4493
Fragments arranged by size:
4493 1533 825 588 183
AccI GT'mk_AC
Cuts at: 3020 4660 3020
Size: 1640 5982
Acc65I G'GTAC_C
Cuts at: 4638 4638
Size: 7622
AclI AA'CG_TT
Cuts at: 7462 7462
Size: 7622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 765 1535 1657 2104 2409 2682 3424 3511
Size: 80 770 122 447 305 273 742 87
Cuts at: 3511 3628 3699 4333 4381 5447 5879 6626 6894
Size: 117 71 634 48 1066 432 747 268
Cuts at: 6894 685
Size: 1413
Fragments arranged by size:
1413 1066 770 747 742 634 447 432
305 273 268 122 117 87 80 71
48
AflIII A'CryG_T
Cuts at: 459 4974 6078 7055 459
Size: 4515 1104 977 1026
Fragments arranged by size:
4515 1104 1026 977
AgeI A'CCGG_T
Cuts at: 6010 6010
Size: 7622
AleI CACnn'nnGTG
Cuts at: 2227 2227
Size: 7622
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4120 4120
Size: 7622
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2503 2535 2989 3021 3892 3924 2503
Size: 32 454 32 871 32 6201
Fragments arranged by size:
6201 871 454 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1254 1353 1800 2217 4597 4927 6494 1254
Size: 99 447 417 2380 330 1567 2382
Fragments arranged by size:
2382 2380 1567 447 417 330 99
ApaI G_GGCC'C
Cuts at: 2873 4266 2873
Size: 1393 6229
ApaLI G'TGCA_C
Cuts at: 6392 6392
Size: 7622
ApoI r'AATT_y
Cuts at: 4626 6975 7231 4626
Size: 2349 256 5017
Fragments arranged by size:
5017 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7622
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7622
AvaI C'yCGr_G
Cuts at: 1107 1173 1738 2788 3054 3133 3538 3671 4280
Size: 66 565 1050 266 79 405 133 609
Cuts at: 4280 4401 4512 4642 1107
Size: 121 111 130 4087
Fragments arranged by size:
4087 1050 609 565 405 266 133 130
121 111 79 66
BamHI G'GATC_C
Cuts at: 1734 4319 4647 1734
Size: 2585 328 4709
Fragments arranged by size:
4709 2585 328
BanI G'GyrC_C
Cuts at: 1147 1764 2452 3106 3836 3891 4638 5052 5300
Size: 617 688 654 730 55 747 414 248
Cuts at: 5300 5335 1147
Size: 35 3434
Fragments arranged by size:
3434 747 730 688 654 617 414 248
55 35
BanII G_rGCy'C
Cuts at: 116 815 1174 2037 2211 2873 3622 3678 4266
Size: 699 359 863 174 662 749 56 588
Cuts at: 4266 4279 4611 4636 5666 116
Size: 13 332 25 1030 2072
Fragments arranged by size:
2072 1030 863 749 699 662 588 359
332 174 56 25 13
BbeI G_GCGC'C
Cuts at: 1768 5056 5304 1768
Size: 3288 248 4086
Fragments arranged by size:
4086 3288 248
BbsI GAAGACnn'nnnn_
Cuts at: 492 1353 2166 2254 2493 2901 3412 3802 492
Size: 861 813 88 239 408 511 390 4312
Fragments arranged by size:
4312 861 813 511 408 390 239 88
BbvCI CC'TCA_GC
Cuts at: 117 3367 4123 117
Size: 3250 756 3616
Fragments arranged by size:
3616 3250 756
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1177 1211 1287 1321 1177
Size: 34 76 34 7478
Fragments arranged by size:
7478 76 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5520 6281 7622 5520
Size: 761 1341 5520
Fragments arranged by size:
5520 1341 761
BclI T'GATC_A
Cuts at: 5142 5142
Size: 7622
BglI GCCn_nnn'nGGC
Cuts at: 257 860 1204 2275 2965 3104 257
Size: 603 344 1071 690 139 4775
Fragments arranged by size:
4775 1071 690 603 344 139
BglII A'GATC_T
Cuts at: 1520 1554 3482 5137 7174 1520
Size: 34 1928 1655 2037 1968
Fragments arranged by size:
2037 1968 1928 1655 34
BlpI GC'TnA_GC
Cuts at: 3776 4734 3776
Size: 958 6664
Bme1580I G_kGCm'C
Cuts at: 253 439 844 1114 2455 2582 2873 3133 3881
Size: 186 405 270 1341 127 291 260 748
Cuts at: 3881 3896 4266 5247 5340 6396 253
Size: 15 370 981 93 1056 1479
Fragments arranged by size:
1479 1341 1056 981 748 405 370 291
270 260 186 127 93 15
BmgBI CAC'GTC
Cuts at: 1392 1890 4470 1392
Size: 498 2580 4544
Fragments arranged by size:
4544 2580 498
BmrI ACTGGGnnnn_n'
Cuts at: 600 1470 4269 4834 4915 5250 7394 600
Size: 870 2799 565 81 335 2144 828
Fragments arranged by size:
2799 2144 870 828 565 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1933 5989 1933
Size: 4056 3566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 543 1042 1117 1639 2293 3466 3490 3667
Size: 285 499 75 522 654 1173 24 177
Cuts at: 3667 4006 4567 258
Size: 339 561 3313
Fragments arranged by size:
3313 1173 654 561 522 499 339 285
177 75 24
Bpu10I CC'TnA_GC
Cuts at: 117 511 787 3367 4123 117
Size: 394 276 2580 756 3616
Fragments arranged by size:
3616 2580 756 394 276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3418 4798 5677 6169 6467 3418
Size: 1380 879 492 298 4573
Fragments arranged by size:
4573 1380 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 201 1276 7326 201
Size: 1075 6050 497
Fragments arranged by size:
6050 1075 497
BsaAI yAC'GTr
Cuts at: 1788 2553 3825 4975 1788
Size: 765 1272 1150 4435
Fragments arranged by size:
4435 1272 1150 765
BsaBI GATnn'nnATC
Cuts at: 6 1078 5136 7173 6
Size: 1072 4058 2037 455
Fragments arranged by size:
4058 2037 1072 455
BsaHI Gr'CG_yC
Cuts at: 602 746 1190 1765 2723 2906 3575 3731 5053
Size: 144 444 575 958 183 669 156 1322
Cuts at: 5053 5301 6003 7499 602
Size: 248 702 1496 725
Fragments arranged by size:
1496 1322 958 725 702 669 575 444
248 183 156 144
BsaWI w'CCGG_w
Cuts at: 1337 1919 2189 4811 5332 6010 6284 6431 6842
Size: 582 270 2622 521 678 274 147 411
Cuts at: 6842 1337
Size: 2117
Fragments arranged by size:
2622 2117 678 582 521 411 274 270
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 541 571 2503 2533 3619 3649 3892 3922 3979
Size: 30 1932 30 1086 30 243 30 57
Cuts at: 3979 4009 541
Size: 30 4154
Fragments arranged by size:
4154 1932 1086 243 57 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 421 1070 2116 2119 2140 2161 2395 2647
Size: 258 649 1046 3 21 21 234 252
Cuts at: 2647 3226 3337 3355 3472 3664 3778 3817 3832
Size: 579 111 18 117 192 114 39 15
Cuts at: 3832 4570 163
Size: 738 3215
Fragments arranged by size:
3215 1046 738 649 579 258 252 234
192 117 114 111 39 21 21 18
15 3
BseYI C'CCAG_C
Cuts at: 358 584 1812 2865 5047 5805 6382 6878 358
Size: 226 1228 1053 2182 758 577 496 1102
Fragments arranged by size:
2182 1228 1102 1053 758 577 496 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1543 1562 1651 1924 3292 3664 4084 4483 1543
Size: 19 89 273 1368 372 420 399 4682
Fragments arranged by size:
4682 1368 420 399 372 273 89 19
BsiEI CG_ry'CG
Cuts at: 104 1860 3336 3959 5164 5210 6418 104
Size: 1756 1476 623 1205 46 1208 1308
Fragments arranged by size:
1756 1476 1308 1208 1205 623 46
BsiHKAI G_wGCw'C
Cuts at: 815 2159 2558 3092 3281 4259 4337 4636 5414
Size: 1344 399 534 189 978 78 299 778
Cuts at: 5414 5604 6396 815
Size: 190 792 2041
Fragments arranged by size:
2041 1344 978 792 778 534 399 299
190 189 78
BsmI GAATG_Cn'
Cuts at: 325 3834 325
Size: 3509 4113
BsmBI CGTCTCn'nnnn_
Cuts at: 3895 4423 3895
Size: 528 7094
Bsp1286I G_dGCh'C
Cuts at: 116 253 439 815 844 1114 1174 2037 2159
Size: 137 186 376 29 270 60 863 122
Cuts at: 2159 2211 2455 2558 2582 2873 3092 3133 3281
Size: 52 244 103 24 291 219 41 148
Cuts at: 3281 3622 3678 3881 3896 4259 4266 4279 4337
Size: 341 56 203 15 363 7 13 58
Cuts at: 4337 4611 4636 5247 5340 5414 5604 5666 6396
Size: 274 25 611 93 74 190 62 730
Cuts at: 6396 116
Size: 1342
Fragments arranged by size:
1342 863 730 611 376 363 341 291
274 270 244 219 203 190 186 148
137 122 103 93 74 62 60 58
56 52 41 29 25 24 15 13
7
BspEI T'CCGG_A
Cuts at: 1337 6842 1337
Size: 5505 2117
BspHI T'CATG_A
Cuts at: 578 749 6798 578
Size: 171 6049 1402
Fragments arranged by size:
6049 1402 171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 747 940 1220 2263 2767 3212 3258 3383 3596
Size: 193 280 1043 504 445 46 125 213
Cuts at: 3596 3645 4505 4524 4672 4886 5188 5569 747
Size: 49 860 19 148 214 302 381 2800
Fragments arranged by size:
2800 1043 860 504 445 381 302 280
214 213 193 148 125 49 46 19
BsrBI CCG'CTC
Cuts at: 50 5914 5968 7452 7617 50
Size: 5864 54 1484 165 55
Fragments arranged by size:
5864 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1193 1505 5534 1193
Size: 312 4029 3281
Fragments arranged by size:
4029 3281 312
BsrFI r'CCGG_y
Cuts at: 742 1859 2410 3159 5620 5801 6010 742
Size: 1117 551 749 2461 181 209 2354
Fragments arranged by size:
2461 2354 1117 749 551 209 181
BsrGI T'GTAC_A
Cuts at: 4379 4379
Size: 7622
BssHII G'CGCG_C
Cuts at: 1754 1754
Size: 7622
BssSI C'ACGA_G
Cuts at: 964 1453 5893 6251 964
Size: 489 4440 358 2335
Fragments arranged by size:
4440 2335 489 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1416 1722 4999 1416
Size: 306 3277 4039
Fragments arranged by size:
4039 3277 306
BstBI TT'CG_AA
Cuts at: 5983 5983
Size: 7622
BstEII G'GTnAC_C
Cuts at: 624 624
Size: 7622
BstYI r'GATC_y
Cuts at: 1 1073 1322 1520 1554 1734 2078 3050 3482
Size: 1072 249 198 34 180 344 972 432
Cuts at: 3482 4319 4647 5114 5137 5472 5718 6730 6816
Size: 837 328 467 23 335 246 1012 86
Cuts at: 6816 6828 7174 1
Size: 12 346 449
Fragments arranged by size:
1072 1012 972 837 467 449 432 346
344 335 328 249 246 198 180 86
34 23 12
Bsu36I CC'TnA_GG
Cuts at: 124 1941 4027 124
Size: 1817 2086 3719
Fragments arranged by size:
3719 2086 1817
BtgI C'CryG_G
Cuts at: 107 658 875 981 1760 1961 2351 2459 2492
Size: 551 217 106 779 201 390 108 33
Cuts at: 2492 3330 3578 3623 3758 3962 3971 4047 4088
Size: 838 248 45 135 204 9 76 41
Cuts at: 4088 4157 107
Size: 69 3572
Fragments arranged by size:
3572 838 779 551 390 248 217 204
201 135 108 106 76 69 45 41
33 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2037 3225 5572 5753 5916 2037
Size: 1188 2347 181 163 3743
Fragments arranged by size:
3743 2347 1188 181 163
BtsI GCAGTG_nn'
Cuts at: 1490 1521 1546 3090 4865 1490
Size: 31 25 1544 1775 4247
Fragments arranged by size:
4247 1775 1544 31 25
DraI TTT'AAA
Cuts at: 4621 4621
Size: 7622
DraIII CAC_nnn'GTG
Cuts at: 443 1788 2183 4169 443
Size: 1345 395 1986 3896
Fragments arranged by size:
3896 1986 1345 395
DrdI GACnn_nn'nnGTC
Cuts at: 5328 6186 5328
Size: 858 6764
EaeI y'GGCC_r
Cuts at: 915 984 1064 1184 1205 1400 1449 2957 3333
Size: 69 80 120 21 195 49 1508 376
Cuts at: 3333 3413 3956 4339 4436 5207 5381 5772 5799
Size: 80 543 383 97 771 174 391 27
Cuts at: 5799 7144 915
Size: 1345 1393
Fragments arranged by size:
1508 1393 1345 771 543 391 383 376
195 174 120 97 80 80 69 49
27 21
EagI C'GGCC_G
Cuts at: 3333 3956 5207 3333
Size: 623 1251 5748
Fragments arranged by size:
5748 1251 623
EarI CTCTTCn'nnn_
Cuts at: 232 661 1618 2195 2428 2620 2653 2746 2926
Size: 429 957 577 233 192 33 93 180
Cuts at: 2926 3214 3388 3604 4198 5645 5855 232
Size: 288 174 216 594 1447 210 1999
Fragments arranged by size:
1999 1447 957 594 577 429 288 233
216 210 192 180 174 93 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2158 3742 4191 5830 6140 6286 7432 2158
Size: 1584 449 1639 310 146 1146 2348
Fragments arranged by size:
2348 1639 1584 1146 449 310 146
EcoICRI GAG'CTC
Cuts at: 813 4634 813
Size: 3821 3801
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 543 685 765 1042 1117 1535 1639 1657
Size: 285 142 80 277 75 418 104 18
Cuts at: 1657 2104 2293 2409 2682 3424 3466 3490 3511
Size: 447 189 116 273 742 42 24 21
Cuts at: 3511 3628 3667 3699 4006 4333 4381 4567 5447
Size: 117 39 32 307 327 48 186 880
Cuts at: 5447 5879 6626 6894 258
Size: 432 747 268 986
Fragments arranged by size:
986 880 747 742 447 432 418 327
307 285 277 273 268 189 186 142
117 116 104 80 75 48 42 39
32 24 21 18
EcoNI CCTnn'n_nnAGG
Cuts at: 691 2326 3319 4327 4585 691
Size: 1635 993 1008 258 3728
Fragments arranged by size:
3728 1635 1008 993 258
EcoO109I rG'GnC_Cy
Cuts at: 447 713 734 861 977 2869 4263 4517 4761
Size: 266 21 127 116 1892 1394 254 244
Cuts at: 4761 447
Size: 3308
Fragments arranged by size:
3308 1892 1394 266 254 244 127 116
21
EcoRI G'AATT_C
Cuts at: 4626 4626
Size: 7622
EcoRV GAT'ATC
Cuts at: 1332 1332
Size: 7622
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2706 2925 5641 2706
Size: 219 2716 4687
Fragments arranged by size:
4687 2716 219
FseI GG_CCGG'CC
Cuts at: 3163 3163
Size: 7622
FspI TGC'GCA
Cuts at: 5403 5403
Size: 7622
HaeII r_GCGC'y
Cuts at: 1746 1768 2140 2991 3309 5056 5304 6326 7305
Size: 22 372 851 318 1747 248 1022 979
Cuts at: 7305 1746
Size: 2063
Fragments arranged by size:
2063 1747 1022 979 851 372 318 248
22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 541 573 674 706 3002 3034 3979 4011 4287
Size: 32 101 32 2296 32 945 32 276
Cuts at: 4287 4319 5464 5494 5496 5526 541
Size: 32 1145 30 2 30 2637
Fragments arranged by size:
2637 2296 1145 945 276 101 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3573 4661 3573
Size: 1088 6534
KasI G'GCGC_C
Cuts at: 1764 5052 5300 1764
Size: 3288 248 4086
Fragments arranged by size:
4086 3288 248
KpnI G_GTAC'C
Cuts at: 4642 4642
Size: 7622
MluI A'CGCG_T
Cuts at: 7055 7055
Size: 7622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1613 6293 6477 1613
Size: 4680 184 2758
Fragments arranged by size:
4680 2758 184
MscI TGG'CCA
Cuts at: 917 1066 1207 1402 1451 2959 5383 917
Size: 149 141 195 49 1508 2424 3156
Fragments arranged by size:
3156 2424 1508 195 149 141 49
MslI CAynn'nnrTG
Cuts at: 964 2227 2884 3244 3827 4375 5611 5738 964
Size: 1263 657 360 583 548 1236 127 2848
Fragments arranged by size:
2848 1263 1236 657 583 548 360 127
MspA1I CmG'CkG
Cuts at: 660 1350 1473 1576 1635 1762 2059 2400 2461
Size: 690 123 103 59 127 297 341 61
Cuts at: 2461 3534 3710 3760 4127 4597 4733 5047 5407
Size: 1073 176 50 367 470 136 314 360
Cuts at: 5407 6420 6665 660
Size: 1013 245 1617
Fragments arranged by size:
1617 1073 1013 690 470 367 360 341
314 297 245 176 136 127 123 103
61 59 50
NaeI GCC'GGC
Cuts at: 3161 5803 3161
Size: 2642 4980
NarI GG'CG_CC
Cuts at: 1765 5053 5301 1765
Size: 3288 248 4086
Fragments arranged by size:
4086 3288 248
NcoI C'CATG_G
Cuts at: 107 1961 3578 3623 3962 3971 4088 4157 107
Size: 1854 1617 45 339 9 117 69 3572
Fragments arranged by size:
3572 1854 1617 339 117 69 45 9
NgoMIV G'CCGG_C
Cuts at: 3159 5801 3159
Size: 2642 4980
NotI GC'GGCC_GC
Cuts at: 3956 3956
Size: 7622
NspI r_CATG'y
Cuts at: 463 2388 4675 5175 6082 463
Size: 1925 2287 500 907 2003
Fragments arranged by size:
2287 2003 1925 907 500
PasI CC'CwG_GG
Cuts at: 3896 3896
Size: 7622
PciI A'CATG_T
Cuts at: 459 6078 459
Size: 5619 2003
PflMI CCAn_nnn'nTGG
Cuts at: 2333 4529 2333
Size: 2196 5426
PfoI T'CCnGG_A
Cuts at: 464 1268 1364 1616 4454 464
Size: 804 96 252 2838 3632
Fragments arranged by size:
3632 2838 804 252 96
PmeI GTTT'AAAC
Cuts at: 4621 4621
Size: 7622
PmlI CAC'GTG
Cuts at: 1788 3825 1788
Size: 2037 5585
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2503 2535 3892 3924 6761 6793 2503
Size: 32 1357 32 2837 32 3332
Fragments arranged by size:
3332 2837 1357 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 447 734 977 447
Size: 287 243 7092
Fragments arranged by size:
7092 287 243
PspOMI G'GGCC_C
Cuts at: 2869 4262 2869
Size: 1393 6229
PstI C_TGCA'G
Cuts at: 1718 1778 2074 2264 2444 2764 3097 3380 4201
Size: 60 296 190 180 320 333 283 821
Cuts at: 4201 4669 5354 1718
Size: 468 685 3986
Fragments arranged by size:
3986 821 685 468 333 320 296 283
190 180 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7622
PvuII CAG'CTG
Cuts at: 1350 1473 1576 1635 4127 5047 5407 1350
Size: 123 103 59 2492 920 360 3565
Fragments arranged by size:
3565 2492 920 360 123 103 59
RsrII CG'GwC_CG
Cuts at: 5817 5817
Size: 7622
SacI G_AGCT'C
Cuts at: 815 4636 815
Size: 3821 3801
SacII CC_GC'GG
Cuts at: 661 1763 2462 3761 661
Size: 1102 699 1299 4522
Fragments arranged by size:
4522 1299 1102 699
SalI G'TCGA_C
Cuts at: 4659 4659
Size: 7622
SanDI GG'GwC_CC
Cuts at: 447 734 977 447
Size: 287 243 7092
Fragments arranged by size:
7092 287 243
SapI GCTCTTCn'nnn_
Cuts at: 661 1618 2620 2653 2746 3214 3388 4198 5645
Size: 957 1002 33 93 468 174 810 1447
Cuts at: 5645 5855 661
Size: 210 2428
Fragments arranged by size:
2428 1447 1002 957 810 468 210 174
93 33
SbfI CC_TGCA'GG
Cuts at: 2764 4669 2764
Size: 1905 5717
ScaI AGT'ACT
Cuts at: 7139 7139
Size: 7622
SfcI C'TryA_G
Cuts at: 33 244 1714 1774 2070 2260 2440 2760 3093
Size: 211 1470 60 296 190 180 320 333
Cuts at: 3093 3376 4197 4665 5350 6343 6534 33
Size: 283 821 468 685 993 191 1121
Fragments arranged by size:
1470 1121 993 821 685 468 333 320
296 283 211 191 190 180 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 2965 2965
Size: 7622
SfoI GGC'GCC
Cuts at: 1766 5054 5302 1766
Size: 3288 248 4086
Fragments arranged by size:
4086 3288 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7622
SgrAI Cr'CCGG_yG
Cuts at: 742 742
Size: 7622
SmaI CCC'GGG
Cuts at: 1109 2790 3056 4282 4644 1109
Size: 1681 266 1226 362 4087
Fragments arranged by size:
4087 1681 1226 362 266
SmlI C'TyrA_G
Cuts at: 3433 4777 5692 6184 6446 3433
Size: 1344 915 492 262 4609
Fragments arranged by size:
4609 1344 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7622
SphI G_CATG'C
Cuts at: 2388 4675 5175 2388
Size: 2287 500 4835
Fragments arranged by size:
4835 2287 500
SrfI GCCC'GGGC
Cuts at: 1109 1109
Size: 7622
StuI AGG'CCT
Cuts at: 686 1414 2239 2317 686
Size: 728 825 78 5991
Fragments arranged by size:
5991 825 728 78
StyI C'CwwG_G
Cuts at: 107 918 1046 1961 2063 2096 2279 2327 2621
Size: 811 128 915 102 33 183 48 294
Cuts at: 2621 3578 3623 3962 3971 4088 4157 4756 5110
Size: 957 45 339 9 117 69 599 354
Cuts at: 5110 107
Size: 2619
Fragments arranged by size:
2619 957 915 811 599 354 339 294
183 128 117 102 69 48 45 33
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2928 5205 5338 5797 6006 2928
Size: 2277 133 459 209 4544
Fragments arranged by size:
4544 2277 459 209 133
TatI w'GTAC_w
Cuts at: 896 2693 4007 4379 7137 896
Size: 1797 1314 372 2758 1381
Fragments arranged by size:
2758 1797 1381 1314 372
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7139 7171 7139
Size: 32 7590
Tth111I GACn'n_nGTC
Cuts at: 496 5419 496
Size: 4923 2699
XbaI T'CTAG_A
Cuts at: 4653 4653
Size: 7622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1086 1444 3969 5117 1086
Size: 358 2525 1148 3591
Fragments arranged by size:
3591 2525 1148 358
XmaI C'CCGG_G
Cuts at: 1107 2788 3054 4280 4642 1107
Size: 1681 266 1226 362 4087
Fragments arranged by size:
4087 1681 1226 362 266
XmnI GAAnn'nnTTC
Cuts at: 1612 1612
Size: 7622
ZraI GAC'GTC
Cuts at: 603 1191 2724 2907 3732 603
Size: 588 1533 183 825 4493
Fragments arranged by size:
4493 1533 825 588 183
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI AvrII BaeI BfrBI BmtI
BsiWI BstXI BstZ17I ClaI CspCI FspAI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PshAI PsiI
PspXI PsrI SexAI SnaBI SspI SwaI XhoI