Restriction map for
pF1KE2516
(Circular) (Six-base) MAPSORT of: pF1KE2516.seq Check: 7169 from: 1 to: 4226
pF1KE2516 4226 bp
With 182 enzymes: SgfI *
February 25, 2014 15:45 ..
AccI GT'mk_AC
Cuts at: 1264 1264
Size: 4226
Acc65I G'GTAC_C
Cuts at: 1242 1242
Size: 4226
AclI AA'CG_TT
Cuts at: 4066 4066
Size: 4226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2483 3230 3498 2051
Size: 432 747 268 2779
Fragments arranged by size:
2779 747 432 268
AflIII A'CryG_T
Cuts at: 1126 1578 2682 3659 1126
Size: 452 1104 977 1693
Fragments arranged by size:
1693 1104 977 452
AgeI A'CCGG_T
Cuts at: 2614 2614
Size: 4226
AlwNI CAG_nnn'CTG
Cuts at: 522 800 1531 3098 522
Size: 278 731 1567 1650
Fragments arranged by size:
1650 1567 731 278
ApaLI G'TGCA_C
Cuts at: 2996 2996
Size: 4226
ApoI r'AATT_y
Cuts at: 1230 3579 3835 1230
Size: 2349 256 1621
Fragments arranged by size:
2349 1621 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4226
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
AvaI C'yCGr_G
Cuts at: 360 527 534 594 1246 360
Size: 167 7 60 652 3340
Fragments arranged by size:
3340 652 167 60 7
BamHI G'GATC_C
Cuts at: 1251 1251
Size: 4226
BanI G'GyrC_C
Cuts at: 565 1242 1656 1904 1939 565
Size: 677 414 248 35 2852
Fragments arranged by size:
2852 677 414 248 35
BanII G_rGCy'C
Cuts at: 215 249 460 1240 2270 215
Size: 34 211 780 1030 2171
Fragments arranged by size:
2171 1030 780 211 34
BbeI G_GCGC'C
Cuts at: 569 1660 1908 569
Size: 1091 248 2887
Fragments arranged by size:
2887 1091 248
BciVI GTATCCnnnnn_n'
Cuts at: 2124 2885 4226 2124
Size: 761 1341 2124
Fragments arranged by size:
2124 1341 761
BclI T'GATC_A
Cuts at: 1746 1746
Size: 4226
BglI GCCn_nnn'nGGC
Cuts at: 432 623 432
Size: 191 4035
BglII A'GATC_T
Cuts at: 1741 3778 1741
Size: 2037 2189
BlpI GC'TnA_GC
Cuts at: 1338 1338
Size: 4226
Bme1580I G_kGCm'C
Cuts at: 884 935 1851 1944 3000 884
Size: 51 916 93 1056 2110
Fragments arranged by size:
2110 1056 916 93 51
BmgBI CAC'GTC
Cuts at: 1127 1127
Size: 4226
BmrI ACTGGGnnnn_n'
Cuts at: 1438 1519 1854 3998 1438
Size: 81 335 2144 1666
Fragments arranged by size:
2144 1666 335 81
BmtI G_CTAG'C
Cuts at: 359 359
Size: 4226
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 4226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 943 482
Size: 461 3765
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1198 1402 2281 2773 3071 1198
Size: 204 879 492 298 2353
Fragments arranged by size:
2353 879 492 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 808 3930 808
Size: 3122 1104
BsaAI yAC'GTr
Cuts at: 1579 1579
Size: 4226
BsaBI GATnn'nnATC
Cuts at: 6 785 1740 3777 6
Size: 779 955 2037 455
Fragments arranged by size:
2037 955 779 455
BsaHI Gr'CG_yC
Cuts at: 254 566 1657 1905 2607 4103 254
Size: 312 1091 248 702 1496 377
Fragments arranged by size:
1496 1091 702 377 312 248
BsaWI w'CCGG_w
Cuts at: 1415 1936 2614 2888 3035 3446 1415
Size: 521 678 274 147 411 2195
Fragments arranged by size:
2195 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 215 245 293 296 323 326 536 566 215
Size: 30 48 3 27 3 210 30 3875
Fragments arranged by size:
3875 210 48 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 317 385 400 409 430 586 758 955
Size: 172 68 15 9 21 156 172 197
Cuts at: 955 145
Size: 3416
Fragments arranged by size:
3416 197 172 172 156 68 21 15
9
BseYI C'CCAG_C
Cuts at: 136 142 1651 2409 2986 3482 136
Size: 6 1509 758 577 496 880
Fragments arranged by size:
1509 880 758 577 496 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 710 710
Size: 4226
BsiEI CG_ry'CG
Cuts at: 104 165 633 1768 1814 3022 104
Size: 61 468 1135 46 1208 1308
Fragments arranged by size:
1308 1208 1135 468 61 46
BsiHKAI G_wGCw'C
Cuts at: 498 1240 2018 2208 3000 498
Size: 742 778 190 792 1724
Fragments arranged by size:
1724 792 778 742 190
BsmI GAATG_Cn'
Cuts at: 1039 1039
Size: 4226
Bsp1286I G_dGCh'C
Cuts at: 215 249 460 498 884 935 1240 1851 1944
Size: 34 211 38 386 51 305 611 93
Cuts at: 1944 2018 2208 2270 3000 215
Size: 74 190 62 730 1441
Fragments arranged by size:
1441 730 611 386 305 211 190 93
74 62 51 38 34
BspEI T'CCGG_A
Cuts at: 3446 3446
Size: 4226
BspHI T'CATG_A
Cuts at: 3402 3402
Size: 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1276 1490 1792 2173 1276
Size: 214 302 381 3329
Fragments arranged by size:
3329 381 302 214
BsrBI CCG'CTC
Cuts at: 50 338 578 1092 2518 2572 4056 4221 50
Size: 288 240 514 1426 54 1484 165 55
Fragments arranged by size:
1484 1426 514 288 240 165 55 54
BsrDI GCAATG_nn'
Cuts at: 915 2138 915
Size: 1223 3003
BsrFI r'CCGG_y
Cuts at: 164 622 2224 2405 2614 164
Size: 458 1602 181 209 1776
Fragments arranged by size:
1776 1602 458 209 181
BsrGI T'GTAC_A
Cuts at: 1110 1110
Size: 4226
BssHII G'CGCG_C
Cuts at: 505 507 505
Size: 2 4224
BssSI C'ACGA_G
Cuts at: 2497 2855 2497
Size: 358 3868
BstAPI GCAn_nnn'nTGC
Cuts at: 493 800 966 1603 493
Size: 307 166 637 3116
Fragments arranged by size:
3116 637 307 166
BstBI TT'CG_AA
Cuts at: 2587 2587
Size: 4226
BstYI r'GATC_y
Cuts at: 1 1116 1251 1718 1741 2076 2322 3334 3420
Size: 1115 135 467 23 335 246 1012 86
Cuts at: 3420 3432 3778 1
Size: 12 346 449
Fragments arranged by size:
1115 1012 467 449 346 335 246 135
86 23 12
BtgI C'CryG_G
Cuts at: 195 308 731 195
Size: 113 423 3690
Fragments arranged by size:
3690 423 113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 906 2176 2357 2520 906
Size: 1270 181 163 2612
Fragments arranged by size:
2612 1270 181 163
BtsI GCAGTG_nn'
Cuts at: 436 1469 436
Size: 1033 3193
DraI TTT'AAA
Cuts at: 1225 1225
Size: 4226
DraIII CAC_nnn'GTG
Cuts at: 765 765
Size: 4226
DrdI GACnn_nn'nnGTC
Cuts at: 1932 2790 1932
Size: 858 3368
EaeI y'GGCC_r
Cuts at: 162 1811 1985 2376 2403 3748 162
Size: 1649 174 391 27 1345 640
Fragments arranged by size:
1649 1345 640 391 174 27
EagI C'GGCC_G
Cuts at: 162 1811 162
Size: 1649 2577
EarI CTCTTCn'nnn_
Cuts at: 398 407 543 2249 2459 398
Size: 9 136 1706 210 2165
Fragments arranged by size:
2165 1706 210 136 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 186 2434 2744 2890 4036 186
Size: 2248 310 146 1146 376
Fragments arranged by size:
2248 1146 376 310 146
EcoICRI GAG'CTC
Cuts at: 1238 1238
Size: 4226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 943 2051 2483 3230 3498 482
Size: 461 1108 432 747 268 1210
Fragments arranged by size:
1210 1108 747 461 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 983 983
Size: 4226
EcoO109I rG'GnC_Cy
Cuts at: 519 918 1365 519
Size: 399 447 3380
Fragments arranged by size:
3380 447 399
EcoRI G'AATT_C
Cuts at: 1230 1230
Size: 4226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 4226
FspI TGC'GCA
Cuts at: 486 2007 486
Size: 1521 2705
HaeII r_GCGC'y
Cuts at: 446 569 1660 1908 2930 3909 446
Size: 123 1091 248 1022 979 763
Fragments arranged by size:
1091 1022 979 763 248 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 296 328 963 995 2068 2098 2100 2130 296
Size: 32 635 32 1073 30 2 30 2392
Fragments arranged by size:
2392 1073 635 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1265 1265
Size: 4226
KasI G'GCGC_C
Cuts at: 565 1656 1904 565
Size: 1091 248 2887
Fragments arranged by size:
2887 1091 248
KpnI G_GTAC'C
Cuts at: 1246 1246
Size: 4226
MluI A'CGCG_T
Cuts at: 3659 3659
Size: 4226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 300 971 2897 3081 300
Size: 671 1926 184 1445
Fragments arranged by size:
1926 1445 671 184
MscI TGG'CCA
Cuts at: 1987 1987
Size: 4226
MslI CAynn'nnrTG
Cuts at: 955 2215 2342 955
Size: 1260 127 2839
Fragments arranged by size:
2839 1260 127
MspA1I CmG'CkG
Cuts at: 197 1337 1651 2011 3024 3269 197
Size: 1140 314 360 1013 245 1154
Fragments arranged by size:
1154 1140 1013 360 314 245
NaeI GCC'GGC
Cuts at: 166 624 2407 166
Size: 458 1783 1985
Fragments arranged by size:
1985 1783 458
NarI GG'CG_CC
Cuts at: 566 1657 1905 566
Size: 1091 248 2887
Fragments arranged by size:
2887 1091 248
NcoI C'CATG_G
Cuts at: 731 731
Size: 4226
NgoMIV G'CCGG_C
Cuts at: 164 622 2405 164
Size: 458 1783 1985
Fragments arranged by size:
1985 1783 458
NheI G'CTAG_C
Cuts at: 355 355
Size: 4226
NspI r_CATG'y
Cuts at: 1279 1779 2686 1279
Size: 500 907 2819
Fragments arranged by size:
2819 907 500
PciI A'CATG_T
Cuts at: 2682 2682
Size: 4226
PflMI CCAn_nnn'nTGG
Cuts at: 282 522 282
Size: 240 3986
PfoI T'CCnGG_A
Cuts at: 833 833
Size: 4226
PmeI GTTT'AAAC
Cuts at: 1225 1225
Size: 4226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 4194
PstI C_TGCA'G
Cuts at: 1273 1958 1273
Size: 685 3541
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4226
PvuII CAG'CTG
Cuts at: 1651 2011 1651
Size: 360 3866
RsrII CG'GwC_CG
Cuts at: 2421 2421
Size: 4226
SacI G_AGCT'C
Cuts at: 1240 1240
Size: 4226
SacII CC_GC'GG
Cuts at: 198 198
Size: 4226
SalI G'TCGA_C
Cuts at: 1263 1263
Size: 4226
SapI GCTCTTCn'nnn_
Cuts at: 2249 2459 2249
Size: 210 4016
SbfI CC_TGCA'GG
Cuts at: 1273 1273
Size: 4226
ScaI AGT'ACT
Cuts at: 3743 3743
Size: 4226
SfcI C'TryA_G
Cuts at: 33 1015 1269 1954 2947 3138 33
Size: 982 254 685 993 191 1121
Fragments arranged by size:
1121 993 982 685 254 191
SfoI GGC'GCC
Cuts at: 567 1658 1906 567
Size: 1091 248 2887
Fragments arranged by size:
2887 1091 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
SmaI CCC'GGG
Cuts at: 529 1248 529
Size: 719 3507
SmlI C'TyrA_G
Cuts at: 1177 1381 2296 2788 3050 1177
Size: 204 915 492 262 2353
Fragments arranged by size:
2353 915 492 262 204
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4226
SphI G_CATG'C
Cuts at: 1279 1779 1279
Size: 500 3726
SrfI GCCC'GGGC
Cuts at: 529 529
Size: 4226
StyI C'CwwG_G
Cuts at: 731 984 1360 1714 731
Size: 253 376 354 3243
Fragments arranged by size:
3243 376 354 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 657 744 1809 1942 2401 2610 657
Size: 87 1065 133 459 209 2273
Fragments arranged by size:
2273 1065 459 209 133 87
TatI w'GTAC_w
Cuts at: 857 1110 3741 857
Size: 253 2631 1342
Fragments arranged by size:
2631 1342 253
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 215 247 293 325 3743 3775 215
Size: 32 46 32 3418 32 666
Fragments arranged by size:
3418 666 46 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2023 2023
Size: 4226
XbaI T'CTAG_A
Cuts at: 1257 1257
Size: 4226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1721 1721
Size: 4226
XmaI C'CCGG_G
Cuts at: 527 1246 527
Size: 719 3507
XmnI GAAnn'nnTTC
Cuts at: 268 268
Size: 4226
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
Bpu10I BsiWI BsmBI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SspI StuI SwaI XhoI ZraI