Restriction map for
pF1KE2508
(Circular) (Six-base) MAPSORT of: pF1KE2508.seq Check: 9718 from: 1 to: 4475
pF1KE2508 4475 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 379 1513 379
Size: 1134 3341
Acc65I G'GTAC_C
Cuts at: 1491 1491
Size: 4475
AclI AA'CG_TT
Cuts at: 4315 4315
Size: 4475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 474 1127 2300 2732 3479 3747 474
Size: 653 1173 432 747 268 1202
Fragments arranged by size:
1202 1173 747 653 432 268
AflIII A'CryG_T
Cuts at: 1827 2931 3908 1827
Size: 1104 977 2394
Fragments arranged by size:
2394 1104 977
AgeI A'CCGG_T
Cuts at: 2863 2863
Size: 4475
AlwNI CAG_nnn'CTG
Cuts at: 1327 1780 3347 1327
Size: 453 1567 2455
Fragments arranged by size:
2455 1567 453
ApaLI G'TGCA_C
Cuts at: 3245 3245
Size: 4475
ApoI r'AATT_y
Cuts at: 1479 3828 4084 1479
Size: 2349 256 1870
Fragments arranged by size:
2349 1870 256
AseI AT'TA_AT
Cuts at: 20 436 508 20
Size: 416 72 3987
Fragments arranged by size:
3987 416 72
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
AvaI C'yCGr_G
Cuts at: 312 1495 312
Size: 1183 3292
BamHI G'GATC_C
Cuts at: 1500 1500
Size: 4475
BanI G'GyrC_C
Cuts at: 1491 1905 2153 2188 1491
Size: 414 248 35 3778
Fragments arranged by size:
3778 414 248 35
BanII G_rGCy'C
Cuts at: 1349 1489 2519 1349
Size: 140 1030 3305
Fragments arranged by size:
3305 1030 140
BbeI G_GCGC'C
Cuts at: 1909 2157 1909
Size: 248 4227
BbsI GAAGACnn'nnnn_
Cuts at: 140 944 1115 1168 140
Size: 804 171 53 3447
Fragments arranged by size:
3447 804 171 53
BciVI GTATCCnnnnn_n'
Cuts at: 591 2373 3134 4475 591
Size: 1782 761 1341 591
Fragments arranged by size:
1782 1341 761 591
BclI T'GATC_A
Cuts at: 1995 1995
Size: 4475
BfrBI ATG'CAT
Cuts at: 390 873 390
Size: 483 3992
BglII A'GATC_T
Cuts at: 1990 4027 1990
Size: 2037 2438
BlpI GC'TnA_GC
Cuts at: 1587 1587
Size: 4475
Bme1580I G_kGCm'C
Cuts at: 2100 2193 3249 2100
Size: 93 1056 3326
Fragments arranged by size:
3326 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 309 1441 1687 1768 2103 4247 309
Size: 1132 246 81 335 2144 537
Fragments arranged by size:
2144 1132 537 335 246 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 4475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1651 2530 3022 3320 1651
Size: 879 492 298 2806
Fragments arranged by size:
2806 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1022 4179 1022
Size: 3157 1318
BsaAI yAC'GTr
Cuts at: 1828 1828
Size: 4475
BsaBI GATnn'nnATC
Cuts at: 6 1989 4026 6
Size: 1983 2037 455
Fragments arranged by size:
2037 1983 455
BsaHI Gr'CG_yC
Cuts at: 1906 2154 2856 4352 1906
Size: 248 702 1496 2029
Fragments arranged by size:
2029 1496 702 248
BsaWI w'CCGG_w
Cuts at: 521 1664 2185 2863 3137 3284 3695 521
Size: 1143 521 678 274 147 411 1301
Fragments arranged by size:
1301 1143 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1405 1435 1405
Size: 30 4445
BseYI C'CCAG_C
Cuts at: 1900 2658 3235 3731 1900
Size: 758 577 496 2644
Fragments arranged by size:
2644 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1429 1429
Size: 4475
BsiEI CG_ry'CG
Cuts at: 104 2017 2063 3271 104
Size: 1913 46 1208 1308
Fragments arranged by size:
1913 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1489 2267 2457 3249 1489
Size: 778 190 792 2715
Fragments arranged by size:
2715 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1349 1489 2100 2193 2267 2457 2519 3249 1349
Size: 140 611 93 74 190 62 730 2575
Fragments arranged by size:
2575 730 611 190 140 93 74 62
BspEI T'CCGG_A
Cuts at: 3695 3695
Size: 4475
BspHI T'CATG_A
Cuts at: 1152 3651 1152
Size: 2499 1976
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1525 1739 2041 2422 1525
Size: 214 302 381 3578
Fragments arranged by size:
3578 381 302 214
BsrBI CCG'CTC
Cuts at: 50 804 2767 2821 4305 4470 50
Size: 754 1963 54 1484 165 55
Fragments arranged by size:
1963 1484 754 165 55 54
BsrDI GCAATG_nn'
Cuts at: 852 1101 2387 852
Size: 249 1286 2940
Fragments arranged by size:
2940 1286 249
BsrFI r'CCGG_y
Cuts at: 2473 2654 2863 2473
Size: 181 209 4085
Fragments arranged by size:
4085 209 181
BsrGI T'GTAC_A
Cuts at: 110 1358 110
Size: 1248 3227
BssSI C'ACGA_G
Cuts at: 2746 3104 2746
Size: 358 4117
BstAPI GCAn_nnn'nTGC
Cuts at: 1852 1852
Size: 4475
BstBI TT'CG_AA
Cuts at: 2836 2836
Size: 4475
BstYI r'GATC_y
Cuts at: 1 1500 1967 1990 2325 2571 3583 3669 3681
Size: 1499 467 23 335 246 1012 86 12
Cuts at: 3681 4027 1
Size: 346 449
Fragments arranged by size:
1499 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2425 2606 2769 2425
Size: 181 163 4131
Fragments arranged by size:
4131 181 163
BtsI GCAGTG_nn'
Cuts at: 214 1718 214
Size: 1504 2971
DraI TTT'AAA
Cuts at: 1474 1474
Size: 4475
DrdI GACnn_nn'nnGTC
Cuts at: 2181 3039 2181
Size: 858 3617
EaeI y'GGCC_r
Cuts at: 2060 2234 2625 2652 3997 2060
Size: 174 391 27 1345 2538
Fragments arranged by size:
2538 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2060 2060
Size: 4475
EarI CTCTTCn'nnn_
Cuts at: 2498 2708 2498
Size: 210 4265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2683 2993 3139 4285 2683
Size: 310 146 1146 2873
Fragments arranged by size:
2873 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1487 1487
Size: 4475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 474 1127 2300 2732 3479 3747 474
Size: 653 1173 432 747 268 1202
Fragments arranged by size:
1202 1173 747 653 432 268
EcoO109I rG'GnC_Cy
Cuts at: 356 1614 356
Size: 1258 3217
EcoRI G'AATT_C
Cuts at: 1479 1479
Size: 4475
EcoRV GAT'ATC
Cuts at: 309 309
Size: 4475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 4475
FspI TGC'GCA
Cuts at: 2256 2256
Size: 4475
HaeII r_GCGC'y
Cuts at: 1909 2157 3179 4158 1909
Size: 248 1022 979 2226
Fragments arranged by size:
2226 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2317 2347 2349 2379 2317
Size: 30 2 30 4413
Fragments arranged by size:
4413 30 30 2
HincII GTy'rAC
Cuts at: 1049 1514 1049
Size: 465 4010
KasI G'GCGC_C
Cuts at: 1905 2153 1905
Size: 248 4227
KpnI G_GTAC'C
Cuts at: 1495 1495
Size: 4475
MluI A'CGCG_T
Cuts at: 3908 3908
Size: 4475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1155 3146 3330 1155
Size: 1991 184 2300
Fragments arranged by size:
2300 1991 184
MscI TGG'CCA
Cuts at: 2236 2236
Size: 4475
MslI CAynn'nnrTG
Cuts at: 455 878 1154 2464 2591 455
Size: 423 276 1310 127 2339
Fragments arranged by size:
2339 1310 423 276 127
MspA1I CmG'CkG
Cuts at: 958 1014 1586 1900 2260 3273 3518 958
Size: 56 572 314 360 1013 245 1915
Fragments arranged by size:
1915 1013 572 360 314 245 56
NaeI GCC'GGC
Cuts at: 2656 2656
Size: 4475
NarI GG'CG_CC
Cuts at: 1906 2154 1906
Size: 248 4227
NgoMIV G'CCGG_C
Cuts at: 2654 2654
Size: 4475
NsiI A_TGCA'T
Cuts at: 392 875 392
Size: 483 3992
NspI r_CATG'y
Cuts at: 1528 2028 2935 1528
Size: 500 907 3068
Fragments arranged by size:
3068 907 500
PciI A'CATG_T
Cuts at: 2931 2931
Size: 4475
PfoI T'CCnGG_A
Cuts at: 1241 1241
Size: 4475
PmeI GTTT'AAAC
Cuts at: 1474 1474
Size: 4475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4443
PpuMI rG'GwC_Cy
Cuts at: 356 356
Size: 4475
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1367 1399 1367
Size: 32 4443
PstI C_TGCA'G
Cuts at: 1522 2207 1522
Size: 685 3790
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4475
PvuII CAG'CTG
Cuts at: 958 1014 1900 2260 958
Size: 56 886 360 3173
Fragments arranged by size:
3173 886 360 56
RsrII CG'GwC_CG
Cuts at: 2670 2670
Size: 4475
SacI G_AGCT'C
Cuts at: 1489 1489
Size: 4475
SalI G'TCGA_C
Cuts at: 1512 1512
Size: 4475
SapI GCTCTTCn'nnn_
Cuts at: 2498 2708 2498
Size: 210 4265
SbfI CC_TGCA'GG
Cuts at: 1522 1522
Size: 4475
ScaI AGT'ACT
Cuts at: 3992 3992
Size: 4475
SfcI C'TryA_G
Cuts at: 33 245 629 1277 1518 2203 3196 3387 33
Size: 212 384 648 241 685 993 191 1121
Fragments arranged by size:
1121 993 685 648 384 241 212 191
SfoI GGC'GCC
Cuts at: 1907 2155 1907
Size: 248 4227
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
SmaI CCC'GGG
Cuts at: 1497 1497
Size: 4475
SmlI C'TyrA_G
Cuts at: 312 1630 2545 3037 3299 312
Size: 1318 915 492 262 1488
Fragments arranged by size:
1488 1318 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4475
SphI G_CATG'C
Cuts at: 1528 2028 1528
Size: 500 3975
SspI AAT'ATT
Cuts at: 446 621 776 1117 446
Size: 175 155 341 3804
Fragments arranged by size:
3804 341 175 155
StyI C'CwwG_G
Cuts at: 1609 1963 1609
Size: 354 4121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2058 2191 2650 2859 2058
Size: 133 459 209 3674
Fragments arranged by size:
3674 459 209 133
TatI w'GTAC_w
Cuts at: 110 1358 3990 110
Size: 1248 2632 595
Fragments arranged by size:
2632 1248 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1405 1437 3992 4024 1405
Size: 32 2555 32 1856
Fragments arranged by size:
2555 1856 32 32
Tth111I GACn'n_nGTC
Cuts at: 2272 2272
Size: 4475
XbaI T'CTAG_A
Cuts at: 1506 1506
Size: 4475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1970 1970
Size: 4475
XhoI C'TCGA_G
Cuts at: 312 312
Size: 4475
XmaI C'CCGG_G
Cuts at: 1495 1495
Size: 4475
XmnI GAAnn'nnTTC
Cuts at: 335 335
Size: 4475
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI BpmI Bpu10I BseRI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PmlI PshAI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI ZraI