Restriction map for
pF1KE2506
(Circular) (Six-base) MAPSORT of: pF1KE2506.seq Check: 9139 from: 1 to: 4481
pF1KE2506 4481 bp
With 182 enzymes: SgfI *
February 25, 2014 15:45 ..
AccI GT'mk_AC
Cuts at: 509 631 1519 509
Size: 122 888 3471
Fragments arranged by size:
3471 888 122
Acc65I G'GTAC_C
Cuts at: 1497 1497
Size: 4481
AclI AA'CG_TT
Cuts at: 4321 4321
Size: 4481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 659 1058 1079 2306 2738 3485 3753 659
Size: 399 21 1227 432 747 268 1387
Fragments arranged by size:
1387 1227 747 432 399 268 21
AflIII A'CryG_T
Cuts at: 504 750 1833 2937 3914 504
Size: 246 1083 1104 977 1071
Fragments arranged by size:
1104 1083 1071 977 246
AgeI A'CCGG_T
Cuts at: 2869 2869
Size: 4481
AhdI GACnn_n'nnGTC
Cuts at: 462 598 462
Size: 136 4345
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 128 160 128
Size: 32 4449
AlwNI CAG_nnn'CTG
Cuts at: 1786 3353 1786
Size: 1567 2914
ApaLI G'TGCA_C
Cuts at: 3251 3251
Size: 4481
ApoI r'AATT_y
Cuts at: 441 1485 3834 4090 441
Size: 1044 2349 256 832
Fragments arranged by size:
2349 1044 832 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4481
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4481
AvaI C'yCGr_G
Cuts at: 1297 1501 1297
Size: 204 4277
BamHI G'GATC_C
Cuts at: 729 1506 729
Size: 777 3704
BanI G'GyrC_C
Cuts at: 1497 1911 2159 2194 1497
Size: 414 248 35 3784
Fragments arranged by size:
3784 414 248 35
BanII G_rGCy'C
Cuts at: 1495 2525 1495
Size: 1030 3451
BbeI G_GCGC'C
Cuts at: 1915 2163 1915
Size: 248 4233
BbsI GAAGACnn'nnnn_
Cuts at: 597 1166 597
Size: 569 3912
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 846 880 846
Size: 34 4447
BciVI GTATCCnnnnn_n'
Cuts at: 676 2379 3140 4481 676
Size: 1703 761 1341 676
Fragments arranged by size:
1703 1341 761 676
BclI T'GATC_A
Cuts at: 1194 1355 2001 1194
Size: 161 646 3674
Fragments arranged by size:
3674 646 161
BglII A'GATC_T
Cuts at: 396 1996 4033 396
Size: 1600 2037 844
Fragments arranged by size:
2037 1600 844
BlpI GC'TnA_GC
Cuts at: 1593 1593
Size: 4481
Bme1580I G_kGCm'C
Cuts at: 2106 2199 3255 2106
Size: 93 1056 3332
Fragments arranged by size:
3332 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1693 1774 2109 4253 1693
Size: 81 335 2144 1921
Fragments arranged by size:
2144 1921 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2848 2848
Size: 4481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 1253 353
Size: 900 3581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1657 2536 3028 3326 1657
Size: 879 492 298 2812
Fragments arranged by size:
2812 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4185 4185
Size: 4481
BsaAI yAC'GTr
Cuts at: 505 1834 505
Size: 1329 3152
BsaBI GATnn'nnATC
Cuts at: 6 1995 4032 6
Size: 1989 2037 455
Fragments arranged by size:
2037 1989 455
BsaHI Gr'CG_yC
Cuts at: 1912 2160 2862 4358 1912
Size: 248 702 1496 2035
Fragments arranged by size:
2035 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1670 2191 2869 3143 3290 3701 1670
Size: 521 678 274 147 411 2450
Fragments arranged by size:
2450 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 605 635 1118 1148 1226 1256 605
Size: 30 483 30 78 30 3830
Fragments arranged by size:
3830 483 78 30 30 30
BseYI C'CCAG_C
Cuts at: 1266 1906 2664 3241 3737 1266
Size: 640 758 577 496 2010
Fragments arranged by size:
2010 758 640 577 496
BsiEI CG_ry'CG
Cuts at: 104 2023 2069 3277 104
Size: 1919 46 1208 1308
Fragments arranged by size:
1919 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1495 2273 2463 3255 1495
Size: 778 190 792 2721
Fragments arranged by size:
2721 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1495 2106 2199 2273 2463 2525 3255 1495
Size: 611 93 74 190 62 730 2721
Fragments arranged by size:
2721 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3701 3701
Size: 4481
BspHI T'CATG_A
Cuts at: 528 3657 528
Size: 3129 1352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1531 1745 2047 2428 1531
Size: 214 302 381 3584
Fragments arranged by size:
3584 381 302 214
BsrBI CCG'CTC
Cuts at: 50 367 2773 2827 4311 4476 50
Size: 317 2406 54 1484 165 55
Fragments arranged by size:
2406 1484 317 165 55 54
BsrDI GCAATG_nn'
Cuts at: 557 1052 2393 557
Size: 495 1341 2645
Fragments arranged by size:
2645 1341 495
BsrFI r'CCGG_y
Cuts at: 2479 2660 2869 2479
Size: 181 209 4091
Fragments arranged by size:
4091 209 181
BssSI C'ACGA_G
Cuts at: 2752 3110 2752
Size: 358 4123
BstAPI GCAn_nnn'nTGC
Cuts at: 1858 1858
Size: 4481
BstBI TT'CG_AA
Cuts at: 377 445 2842 377
Size: 68 2397 2016
Fragments arranged by size:
2397 2016 68
BstXI CCAn_nnnn'nTGG
Cuts at: 817 817
Size: 4481
BstYI r'GATC_y
Cuts at: 1 215 396 729 1506 1973 1996 2331 2577
Size: 214 181 333 777 467 23 335 246
Cuts at: 2577 3589 3675 3687 4033 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 777 467 449 346 335 333 246
214 181 86 23 12
BstZ17I GTA'TAC
Cuts at: 632 632
Size: 4481
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2431 2612 2775 2431
Size: 181 163 4137
Fragments arranged by size:
4137 181 163
BtsI GCAGTG_nn'
Cuts at: 1724 1724
Size: 4481
DraI TTT'AAA
Cuts at: 996 1480 996
Size: 484 3997
DrdI GACnn_nn'nnGTC
Cuts at: 2187 3045 2187
Size: 858 3623
EaeI y'GGCC_r
Cuts at: 362 1284 2066 2240 2631 2658 4003 362
Size: 922 782 174 391 27 1345 840
Fragments arranged by size:
1345 922 840 782 391 174 27
EagI C'GGCC_G
Cuts at: 2066 2066
Size: 4481
EarI CTCTTCn'nnn_
Cuts at: 158 635 1456 2504 2714 158
Size: 477 821 1048 210 1925
Fragments arranged by size:
1925 1048 821 477 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2689 2999 3145 4291 2689
Size: 310 146 1146 2879
Fragments arranged by size:
2879 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1493 1493
Size: 4481
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 659 1058 1079 1253 2306 2738 3485 3753
Size: 306 399 21 174 1053 432 747 268
Cuts at: 3753 353
Size: 1081
Fragments arranged by size:
1081 1053 747 432 399 306 268 174
21
EcoO109I rG'GnC_Cy
Cuts at: 1008 1620 1008
Size: 612 3869
EcoRI G'AATT_C
Cuts at: 1485 1485
Size: 4481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 349 2500 349
Size: 2151 2330
FspI TGC'GCA
Cuts at: 2262 2262
Size: 4481
HaeII r_GCGC'y
Cuts at: 1915 2163 3185 4164 1915
Size: 248 1022 979 2232
Fragments arranged by size:
2232 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 351 383 448 480 584 616 1226 1258 2323
Size: 32 65 32 104 32 610 32 1065
Cuts at: 2323 2353 2355 2385 351
Size: 30 2 30 2447
Fragments arranged by size:
2447 1065 610 104 65 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1520 1520
Size: 4481
HindIII A'AGCT_T
Cuts at: 908 908
Size: 4481
KasI G'GCGC_C
Cuts at: 1911 2159 1911
Size: 248 4233
KpnI G_GTAC'C
Cuts at: 1501 1501
Size: 4481
MluI A'CGCG_T
Cuts at: 3914 3914
Size: 4481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3152 3336 3152
Size: 184 4297
MscI TGG'CCA
Cuts at: 1286 2242 1286
Size: 956 3525
MslI CAynn'nnrTG
Cuts at: 2470 2597 2470
Size: 127 4354
MspA1I CmG'CkG
Cuts at: 1592 1906 2266 3279 3524 1592
Size: 314 360 1013 245 2549
Fragments arranged by size:
2549 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2662 2662
Size: 4481
NarI GG'CG_CC
Cuts at: 1912 2160 1912
Size: 248 4233
NgoMIV G'CCGG_C
Cuts at: 2660 2660
Size: 4481
NspI r_CATG'y
Cuts at: 678 1534 2034 2941 678
Size: 856 500 907 2218
Fragments arranged by size:
2218 907 856 500
PciI A'CATG_T
Cuts at: 2937 2937
Size: 4481
PflMI CCAn_nnn'nTGG
Cuts at: 1266 1266
Size: 4481
PfoI T'CCnGG_A
Cuts at: 1107 1107
Size: 4481
PmeI GTTT'AAAC
Cuts at: 1480 1480
Size: 4481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 4449
PspXI vC'TCGA_Gb
Cuts at: 1297 1297
Size: 4481
PstI C_TGCA'G
Cuts at: 1528 2213 1528
Size: 685 3796
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4481
PvuII CAG'CTG
Cuts at: 1906 2266 1906
Size: 360 4121
RsrII CG'GwC_CG
Cuts at: 2676 2676
Size: 4481
SacI G_AGCT'C
Cuts at: 1495 1495
Size: 4481
SalI G'TCGA_C
Cuts at: 1518 1518
Size: 4481
SapI GCTCTTCn'nnn_
Cuts at: 635 2504 2714 635
Size: 1869 210 2402
Fragments arranged by size:
2402 1869 210
SbfI CC_TGCA'GG
Cuts at: 1528 1528
Size: 4481
ScaI AGT'ACT
Cuts at: 3998 3998
Size: 4481
SfcI C'TryA_G
Cuts at: 33 968 1524 2209 3202 3393 33
Size: 935 556 685 993 191 1121
Fragments arranged by size:
1121 993 935 685 556 191
SfoI GGC'GCC
Cuts at: 1913 2161 1913
Size: 248 4233
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4481
SmaI CCC'GGG
Cuts at: 1503 1503
Size: 4481
SmlI C'TyrA_G
Cuts at: 1297 1636 2551 3043 3305 1297
Size: 339 915 492 262 2473
Fragments arranged by size:
2473 915 492 339 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4481
SphI G_CATG'C
Cuts at: 1534 2034 1534
Size: 500 3981
SspI AAT'ATT
Cuts at: 540 902 540
Size: 362 4119
StyI C'CwwG_G
Cuts at: 609 1615 1969 609
Size: 1006 354 3121
Fragments arranged by size:
3121 1006 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2064 2197 2656 2865 2064
Size: 133 459 209 3680
Fragments arranged by size:
3680 459 209 133
TatI w'GTAC_w
Cuts at: 802 3996 802
Size: 3194 1287
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 927 959 3998 4030 927
Size: 32 3039 32 1378
Fragments arranged by size:
3039 1378 32 32
Tth111I GACn'n_nGTC
Cuts at: 2278 2278
Size: 4481
XbaI T'CTAG_A
Cuts at: 1512 1512
Size: 4481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 817 1976 817
Size: 1159 3322
XhoI C'TCGA_G
Cuts at: 1297 1297
Size: 4481
XmaI C'CCGG_G
Cuts at: 1501 1501
Size: 4481
XmnI GAAnn'nnTTC
Cuts at: 329 329
Size: 4481
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI ZraI