Restriction map for
pF1KE2503
(Circular) (Six-base) MAPSORT of: pF1KE2503.seq Check: 9754 from: 1 to: 5006
pF1KE2503 5006 bp
With 182 enzymes: SgfI *
February 16, 2012 13:35 ..
AccI GT'mk_AC
Cuts at: 450 2044 450
Size: 1594 3412
Acc65I G'GTAC_C
Cuts at: 2022 2022
Size: 5006
AclI AA'CG_TT
Cuts at: 681 4846 681
Size: 4165 841
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 1201 1243 1318 2831 3263 4010 4278 220
Size: 981 42 75 1513 432 747 268 948
Fragments arranged by size:
1513 981 948 747 432 268 75 42
AflIII A'CryG_T
Cuts at: 834 2358 3462 4439 834
Size: 1524 1104 977 1401
Fragments arranged by size:
1524 1401 1104 977
AgeI A'CCGG_T
Cuts at: 3394 3394
Size: 5006
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 418 450 418
Size: 32 4974
AlwNI CAG_nnn'CTG
Cuts at: 154 822 1266 1429 1952 2311 3878 154
Size: 668 444 163 523 359 1567 1282
Fragments arranged by size:
1567 1282 668 523 444 359 163
ApaI G_GGCC'C
Cuts at: 918 918
Size: 5006
ApaLI G'TGCA_C
Cuts at: 3776 3776
Size: 5006
ApoI r'AATT_y
Cuts at: 1711 2010 4359 4615 1711
Size: 299 2349 256 2102
Fragments arranged by size:
2349 2102 299 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5006
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
AvaI C'yCGr_G
Cuts at: 429 762 1777 2026 429
Size: 333 1015 249 3409
Fragments arranged by size:
3409 1015 333 249
BamHI G'GATC_C
Cuts at: 2031 2031
Size: 5006
BanI G'GyrC_C
Cuts at: 286 2022 2436 2684 2719 286
Size: 1736 414 248 35 2573
Fragments arranged by size:
2573 1736 414 248 35
BanII G_rGCy'C
Cuts at: 148 400 918 1133 1761 1778 2020 3050 148
Size: 252 518 215 628 17 242 1030 2104
Fragments arranged by size:
2104 1030 628 518 252 242 215 17
BbeI G_GCGC'C
Cuts at: 2440 2688 2440
Size: 248 4758
BciVI GTATCCnnnnn_n'
Cuts at: 2904 3665 5006 2904
Size: 761 1341 2904
Fragments arranged by size:
2904 1341 761
BclI T'GATC_A
Cuts at: 2526 2526
Size: 5006
BglII A'GATC_T
Cuts at: 2521 4558 2521
Size: 2037 2969
BlpI GC'TnA_GC
Cuts at: 1043 2118 1043
Size: 1075 3931
Bme1580I G_kGCm'C
Cuts at: 291 918 1706 2631 2724 3780 291
Size: 627 788 925 93 1056 1517
Fragments arranged by size:
1517 1056 925 788 627 93
BmrI ACTGGGnnnn_n'
Cuts at: 292 912 1439 1742 2218 2299 2634 4778 292
Size: 620 527 303 476 81 335 2144 520
Fragments arranged by size:
2144 620 527 520 476 335 303 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3373 3373
Size: 5006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 895 1027 1117 1132 1483 1676 229
Size: 666 132 90 15 351 193 3559
Fragments arranged by size:
3559 666 351 193 132 90 15
Bpu10I CC'TnA_GC
Cuts at: 1139 1139
Size: 5006
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 2182 3061 3553 3851 769
Size: 1413 879 492 298 1924
Fragments arranged by size:
1924 1413 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 719 4710 719
Size: 3991 1015
BsaAI yAC'GTr
Cuts at: 837 2359 837
Size: 1522 3484
BsaBI GATnn'nnATC
Cuts at: 6 989 2520 4557 6
Size: 983 1531 2037 455
Fragments arranged by size:
2037 1531 983 455
BsaHI Gr'CG_yC
Cuts at: 2437 2685 3387 4883 2437
Size: 248 702 1496 2560
Fragments arranged by size:
2560 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1523 2195 2716 3394 3668 3815 4226 1523
Size: 672 521 678 274 147 411 2303
Fragments arranged by size:
2303 678 672 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 222 252 1658 1688 222
Size: 30 1406 30 3540
Fragments arranged by size:
3540 1406 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 354 363 366 985 1015 1026 1222 1306
Size: 98 9 3 619 30 11 196 84
Cuts at: 1306 1480 1616 1649 256
Size: 174 136 33 3613
Fragments arranged by size:
3613 619 196 174 136 98 84 33
30 11 9 3
BseYI C'CCAG_C
Cuts at: 322 1065 1359 1416 1761 1973 2431 3189 3766
Size: 743 294 57 345 212 458 758 577
Cuts at: 3766 4262 322
Size: 496 1066
Fragments arranged by size:
1066 758 743 577 496 458 345 294
212 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 255 1823 1868 1918 200
Size: 55 1568 45 50 3288
Fragments arranged by size:
3288 1568 55 50 45
BsiEI CG_ry'CG
Cuts at: 104 672 1391 2548 2594 3802 104
Size: 568 719 1157 46 1208 1308
Fragments arranged by size:
1308 1208 1157 719 568 46
BsiHKAI G_wGCw'C
Cuts at: 1133 1778 1934 2020 2798 2988 3780 1133
Size: 645 156 86 778 190 792 2359
Fragments arranged by size:
2359 792 778 645 190 156 86
BsmBI CGTCTCn'nnnn_
Cuts at: 1369 1369
Size: 5006
Bsp1286I G_dGCh'C
Cuts at: 148 291 400 918 1133 1706 1761 1778 1934
Size: 143 109 518 215 573 55 17 156
Cuts at: 1934 2020 2631 2724 2798 2988 3050 3780 148
Size: 86 611 93 74 190 62 730 1374
Fragments arranged by size:
1374 730 611 573 518 215 190 156
143 109 93 86 74 62 55 17
BspEI T'CCGG_A
Cuts at: 4226 4226
Size: 5006
BspHI T'CATG_A
Cuts at: 4182 4182
Size: 5006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2056 2270 2572 2953 2056
Size: 214 302 381 4109
Fragments arranged by size:
4109 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3298 3352 4836 5001 50
Size: 3248 54 1484 165 55
Fragments arranged by size:
3248 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2918 2918
Size: 5006
BsrFI r'CCGG_y
Cuts at: 667 3004 3185 3394 667
Size: 2337 181 209 2279
Fragments arranged by size:
2337 2279 209 181
BssSI C'ACGA_G
Cuts at: 1303 3277 3635 1303
Size: 1974 358 2674
Fragments arranged by size:
2674 1974 358
BstAPI GCAn_nnn'nTGC
Cuts at: 822 1952 2383 822
Size: 1130 431 3445
Fragments arranged by size:
3445 1130 431
BstBI TT'CG_AA
Cuts at: 3367 3367
Size: 5006
BstXI CCAn_nnnn'nTGG
Cuts at: 663 1830 663
Size: 1167 3839
BstYI r'GATC_y
Cuts at: 1 204 660 2031 2498 2521 2856 3102 4114
Size: 203 456 1371 467 23 335 246 1012
Cuts at: 4114 4200 4212 4558 1
Size: 86 12 346 449
Fragments arranged by size:
1371 1012 467 456 449 346 335 246
203 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1099 1422 1480 1099
Size: 323 58 4625
Fragments arranged by size:
4625 323 58
BtgI C'CryG_G
Cuts at: 1176 1176
Size: 5006
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 671 1537 2956 3137 3300 671
Size: 866 1419 181 163 2377
Fragments arranged by size:
2377 1419 866 181 163
BtsI GCAGTG_nn'
Cuts at: 243 1048 1076 1318 2249 243
Size: 805 28 242 931 3000
Fragments arranged by size:
3000 931 805 242 28
DraI TTT'AAA
Cuts at: 2005 2005
Size: 5006
DrdI GACnn_nn'nnGTC
Cuts at: 2712 3570 2712
Size: 858 4148
EaeI y'GGCC_r
Cuts at: 665 669 1268 1388 1718 2591 2765 3156 3183
Size: 4 599 120 330 873 174 391 27
Cuts at: 3183 4528 665
Size: 1345 1143
Fragments arranged by size:
1345 1143 873 599 391 330 174 120
27 4
EagI C'GGCC_G
Cuts at: 669 1388 2591 669
Size: 719 1203 3084
Fragments arranged by size:
3084 1203 719
EarI CTCTTCn'nnn_
Cuts at: 644 1246 3029 3239 644
Size: 602 1783 210 2411
Fragments arranged by size:
2411 1783 602 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 243 357 1489 3214 3524 3670 4816 243
Size: 114 1132 1725 310 146 1146 433
Fragments arranged by size:
1725 1146 1132 433 310 146 114
EcoICRI GAG'CTC
Cuts at: 1131 1776 2018 1131
Size: 645 242 4119
Fragments arranged by size:
4119 645 242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 229 895 1027 1117 1132 1201 1243 1318
Size: 9 666 132 90 15 69 42 75
Cuts at: 1318 1483 1676 2831 3263 4010 4278 220
Size: 165 193 1155 432 747 268 948
Fragments arranged by size:
1155 948 747 666 432 268 193 165
132 90 75 69 42 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1102 1462 1841 1102
Size: 360 379 4267
Fragments arranged by size:
4267 379 360
EcoO109I rG'GnC_Cy
Cuts at: 914 2145 914
Size: 1231 3775
EcoRI G'AATT_C
Cuts at: 2010 2010
Size: 5006
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3025 3025
Size: 5006
FseI GG_CCGG'CC
Cuts at: 671 671
Size: 5006
FspI TGC'GCA
Cuts at: 2787 2787
Size: 5006
HaeII r_GCGC'y
Cuts at: 2440 2688 3710 4689 2440
Size: 248 1022 979 2757
Fragments arranged by size:
2757 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 745 777 1524 1556 2848 2878 2880
Size: 32 559 32 747 32 1292 30 2
Cuts at: 2880 2910 154
Size: 30 2250
Fragments arranged by size:
2250 1292 747 559 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 451 2045 451
Size: 1594 3412
KasI G'GCGC_C
Cuts at: 2436 2684 2436
Size: 248 4758
KpnI G_GTAC'C
Cuts at: 2026 2026
Size: 5006
MluI A'CGCG_T
Cuts at: 4439 4439
Size: 5006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 119 284 1307 3677 3861 119
Size: 165 1023 2370 184 1264
Fragments arranged by size:
2370 1264 1023 184 165
MscI TGG'CCA
Cuts at: 2767 2767
Size: 5006
MslI CAynn'nnrTG
Cuts at: 174 2995 3122 174
Size: 2821 127 2058
Fragments arranged by size:
2821 2058 127
MspA1I CmG'CkG
Cuts at: 276 301 507 873 1266 1723 2117 2431 2791
Size: 25 206 366 393 457 394 314 360
Cuts at: 2791 3804 4049 276
Size: 1013 245 1233
Fragments arranged by size:
1233 1013 457 394 393 366 360 314
245 206 25
NaeI GCC'GGC
Cuts at: 669 3187 669
Size: 2518 2488
NarI GG'CG_CC
Cuts at: 2437 2685 2437
Size: 248 4758
NgoMIV G'CCGG_C
Cuts at: 667 3185 667
Size: 2518 2488
NruI TCG'CGA
Cuts at: 1199 1199
Size: 5006
NspI r_CATG'y
Cuts at: 388 1492 2059 2559 3466 388
Size: 1104 567 500 907 1928
Fragments arranged by size:
1928 1104 907 567 500
PciI A'CATG_T
Cuts at: 3462 3462
Size: 5006
PflMI CCAn_nnn'nTGG
Cuts at: 121 1371 121
Size: 1250 3756
PfoI T'CCnGG_A
Cuts at: 1460 1752 1460
Size: 292 4714
PmeI GTTT'AAAC
Cuts at: 2005 2005
Size: 5006
PmlI CAC'GTG
Cuts at: 837 837
Size: 5006
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4145 4177 4145
Size: 32 4974
PspOMI G'GGCC_C
Cuts at: 914 914
Size: 5006
PspXI vC'TCGA_Gb
Cuts at: 429 429
Size: 5006
PstI C_TGCA'G
Cuts at: 1325 1352 1665 2053 2738 1325
Size: 27 313 388 685 3593
Fragments arranged by size:
3593 685 388 313 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5006
PvuII CAG'CTG
Cuts at: 301 507 873 1266 2431 2791 301
Size: 206 366 393 1165 360 2516
Fragments arranged by size:
2516 1165 393 366 360 206
RsrII CG'GwC_CG
Cuts at: 3201 3201
Size: 5006
SacI G_AGCT'C
Cuts at: 1133 1778 2020 1133
Size: 645 242 4119
Fragments arranged by size:
4119 645 242
SalI G'TCGA_C
Cuts at: 449 2043 449
Size: 1594 3412
SapI GCTCTTCn'nnn_
Cuts at: 3029 3239 3029
Size: 210 4796
SbfI CC_TGCA'GG
Cuts at: 2053 2053
Size: 5006
ScaI AGT'ACT
Cuts at: 4523 4523
Size: 5006
SfcI C'TryA_G
Cuts at: 33 1321 1348 1661 2049 2734 3727 3918 33
Size: 1288 27 313 388 685 993 191 1121
Fragments arranged by size:
1288 1121 993 685 388 313 191 27
SfoI GGC'GCC
Cuts at: 2438 2686 2438
Size: 248 4758
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
SmaI CCC'GGG
Cuts at: 2028 2028
Size: 5006
SmlI C'TyrA_G
Cuts at: 429 762 784 2161 3076 3568 3830 429
Size: 333 22 1377 915 492 262 1605
Fragments arranged by size:
1605 1377 915 492 333 262 22
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5006
SphI G_CATG'C
Cuts at: 388 2059 2559 388
Size: 1671 500 2835
Fragments arranged by size:
2835 1671 500
StyI C'CwwG_G
Cuts at: 656 1905 2140 2494 656
Size: 1249 235 354 3168
Fragments arranged by size:
3168 1249 354 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 274 1949 2589 2722 3181 3390 274
Size: 1675 640 133 459 209 1890
Fragments arranged by size:
1890 1675 640 459 209 133
TatI w'GTAC_w
Cuts at: 4521 4521
Size: 5006
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 222 254 4523 4555 222
Size: 32 4269 32 673
Fragments arranged by size:
4269 673 32 32
Tth111I GACn'n_nGTC
Cuts at: 2803 2803
Size: 5006
XbaI T'CTAG_A
Cuts at: 2037 2037
Size: 5006
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1615 1970 2501 1615
Size: 355 531 4120
Fragments arranged by size:
4120 531 355
XhoI C'TCGA_G
Cuts at: 429 762 429
Size: 333 4673
XmaI C'CCGG_G
Cuts at: 2026 2026
Size: 5006
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NruI NspI PciI PflMI PfoI
PmeI PmlI PpiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NsiI PacI PasI PpuMI PshAI
PsiI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XmnI ZraI