Restriction map for
pF1KE2501
(Circular) (Six-base) MAPSORT of: pF1KE2501.seq Check: 2128 from: 1 to: 4079
pF1KE2501 4079 bp
With 182 enzymes: SgfI *
February 16, 2012 13:35 ..
AccI GT'mk_AC
Cuts at: 212 1117 212
Size: 905 3174
Acc65I G'GTAC_C
Cuts at: 1095 1095
Size: 4079
AclI AA'CG_TT
Cuts at: 3919 3919
Size: 4079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 900 1904 2336 3083 3351 900
Size: 1004 432 747 268 1628
Fragments arranged by size:
1628 1004 747 432 268
AflIII A'CryG_T
Cuts at: 170 1431 2535 3512 170
Size: 1261 1104 977 737
Fragments arranged by size:
1261 1104 977 737
AgeI A'CCGG_T
Cuts at: 2467 2467
Size: 4079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 256 505 256
Size: 249 3830
AlwNI CAG_nnn'CTG
Cuts at: 516 627 835 1384 2951 516
Size: 111 208 549 1567 1644
Fragments arranged by size:
1644 1567 549 208 111
ApaI G_GGCC'C
Cuts at: 821 821
Size: 4079
ApaLI G'TGCA_C
Cuts at: 2849 2849
Size: 4079
ApoI r'AATT_y
Cuts at: 1083 3432 3688 1083
Size: 2349 256 1474
Fragments arranged by size:
2349 1474 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4079
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
AvaI C'yCGr_G
Cuts at: 813 1062 1099 813
Size: 249 37 3793
Fragments arranged by size:
3793 249 37
BamHI G'GATC_C
Cuts at: 1104 1104
Size: 4079
BanI G'GyrC_C
Cuts at: 1095 1509 1757 1792 1095
Size: 414 248 35 3382
Fragments arranged by size:
3382 414 248 35
BanII G_rGCy'C
Cuts at: 395 821 1093 2123 395
Size: 426 272 1030 2351
Fragments arranged by size:
2351 1030 426 272
BbeI G_GCGC'C
Cuts at: 1513 1761 1513
Size: 248 3831
BciVI GTATCCnnnnn_n'
Cuts at: 1977 2738 4079 1977
Size: 761 1341 1977
Fragments arranged by size:
1977 1341 761
BclI T'GATC_A
Cuts at: 1599 1599
Size: 4079
BglII A'GATC_T
Cuts at: 1594 3631 1594
Size: 2037 2042
BlpI GC'TnA_GC
Cuts at: 459 1191 459
Size: 732 3347
Bme1580I G_kGCm'C
Cuts at: 494 790 821 1704 1797 2853 494
Size: 296 31 883 93 1056 1720
Fragments arranged by size:
1720 1056 883 296 93 31
BmrI ACTGGGnnnn_n'
Cuts at: 1080 1291 1372 1707 3851 1080
Size: 211 81 335 2144 1308
Fragments arranged by size:
2144 1308 335 211 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 780 2446 780
Size: 1666 2413
Bpu10I CC'TnA_GC
Cuts at: 1049 1049
Size: 4079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1255 2134 2626 2924 1012
Size: 243 879 492 298 2167
Fragments arranged by size:
2167 879 492 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 3783 3783
Size: 4079
BsaAI yAC'GTr
Cuts at: 171 1432 171
Size: 1261 2818
BsaBI GATnn'nnATC
Cuts at: 6 1593 3630 6
Size: 1587 2037 455
Fragments arranged by size:
2037 1587 455
BsaHI Gr'CG_yC
Cuts at: 1510 1758 2460 3956 1510
Size: 248 702 1496 1633
Fragments arranged by size:
1633 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1268 1789 2467 2741 2888 3299 1268
Size: 521 678 274 147 411 2048
Fragments arranged by size:
2048 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1025 1055 1025
Size: 30 4049
BseYI C'CCAG_C
Cuts at: 509 1504 2262 2839 3335 509
Size: 995 758 577 496 1253
Fragments arranged by size:
1253 995 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 901
Size: 4079
BsiEI CG_ry'CG
Cuts at: 104 1621 1667 2875 104
Size: 1517 46 1208 1308
Fragments arranged by size:
1517 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 395 1093 1871 2061 2853 395
Size: 698 778 190 792 1621
Fragments arranged by size:
1621 792 778 698 190
Bsp1286I G_dGCh'C
Cuts at: 395 494 790 821 1093 1704 1797 1871 2061
Size: 99 296 31 272 611 93 74 190
Cuts at: 2061 2123 2853 395
Size: 62 730 1621
Fragments arranged by size:
1621 730 611 296 272 190 99 93
74 62 31
BspEI T'CCGG_A
Cuts at: 3299 3299
Size: 4079
BspHI T'CATG_A
Cuts at: 953 3255 953
Size: 2302 1777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 873 1129 1343 1645 2026 873
Size: 256 214 302 381 2926
Fragments arranged by size:
2926 381 302 256 214
BsrBI CCG'CTC
Cuts at: 50 2371 2425 3909 4074 50
Size: 2321 54 1484 165 55
Fragments arranged by size:
2321 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 191 1991 191
Size: 1800 2279
BsrFI r'CCGG_y
Cuts at: 2077 2258 2467 2077
Size: 181 209 3689
Fragments arranged by size:
3689 209 181
BssSI C'ACGA_G
Cuts at: 2350 2708 2350
Size: 358 3721
BstAPI GCAn_nnn'nTGC
Cuts at: 627 835 1456 627
Size: 208 621 3250
Fragments arranged by size:
3250 621 208
BstBI TT'CG_AA
Cuts at: 2440 2440
Size: 4079
BstXI CCAn_nnnn'nTGG
Cuts at: 858 858
Size: 4079
BstYI r'GATC_y
Cuts at: 1 296 623 1104 1571 1594 1929 2175 3187
Size: 295 327 481 467 23 335 246 1012
Cuts at: 3187 3273 3285 3631 1
Size: 86 12 346 449
Fragments arranged by size:
1012 481 467 449 346 335 327 295
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 779 779
Size: 4079
BtgI C'CryG_G
Cuts at: 107 270 857 107
Size: 163 587 3329
Fragments arranged by size:
3329 587 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2029 2210 2373 2029
Size: 181 163 3735
Fragments arranged by size:
3735 181 163
BtsI GCAGTG_nn'
Cuts at: 673 833 1322 673
Size: 160 489 3430
Fragments arranged by size:
3430 489 160
DraI TTT'AAA
Cuts at: 1078 1078
Size: 4079
DrdI GACnn_nn'nnGTC
Cuts at: 1785 2643 1785
Size: 858 3221
EaeI y'GGCC_r
Cuts at: 366 597 968 1664 1838 2229 2256 3601 366
Size: 231 371 696 174 391 27 1345 844
Fragments arranged by size:
1345 844 696 391 371 231 174 27
EagI C'GGCC_G
Cuts at: 1664 1664
Size: 4079
EarI CTCTTCn'nnn_
Cuts at: 253 2102 2312 253
Size: 1849 210 2020
Fragments arranged by size:
2020 1849 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2287 2597 2743 3889 2287
Size: 310 146 1146 2477
Fragments arranged by size:
2477 1146 310 146
EcoICRI GAG'CTC
Cuts at: 393 1091 393
Size: 698 3381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 900 1904 2336 3083 3351 900
Size: 1004 432 747 268 1628
Fragments arranged by size:
1628 1004 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 955 955
Size: 4079
EcoO109I rG'GnC_Cy
Cuts at: 275 817 818 940 948 960 1218 275
Size: 542 1 122 8 12 258 3136
Fragments arranged by size:
3136 542 258 122 12 8 1
EcoRI G'AATT_C
Cuts at: 1083 1083
Size: 4079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 4079
FspI TGC'GCA
Cuts at: 1860 1860
Size: 4079
HaeII r_GCGC'y
Cuts at: 1513 1761 2783 3762 1513
Size: 248 1022 979 1830
Fragments arranged by size:
1830 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 490 522 1921 1951 1953 1983 490
Size: 32 1399 30 2 30 2586
Fragments arranged by size:
2586 1399 32 30 30 2
HincII GTy'rAC
Cuts at: 1118 1118
Size: 4079
KasI G'GCGC_C
Cuts at: 1509 1757 1509
Size: 248 3831
KpnI G_GTAC'C
Cuts at: 1099 1099
Size: 4079
MluI A'CGCG_T
Cuts at: 3512 3512
Size: 4079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1068 2750 2934 1068
Size: 1682 184 2213
Fragments arranged by size:
2213 1682 184
MscI TGG'CCA
Cuts at: 368 599 1840 368
Size: 231 1241 2607
Fragments arranged by size:
2607 1241 231
MslI CAynn'nnrTG
Cuts at: 487 725 1009 2068 2195 487
Size: 238 284 1059 127 2371
Fragments arranged by size:
2371 1059 284 238 127
MspA1I CmG'CkG
Cuts at: 924 1190 1504 1864 2877 3122 924
Size: 266 314 360 1013 245 1881
Fragments arranged by size:
1881 1013 360 314 266 245
NaeI GCC'GGC
Cuts at: 2260 2260
Size: 4079
NarI GG'CG_CC
Cuts at: 1510 1758 1510
Size: 248 3831
NcoI C'CATG_G
Cuts at: 107 270 857 107
Size: 163 587 3329
Fragments arranged by size:
3329 587 163
NgoMIV G'CCGG_C
Cuts at: 2258 2258
Size: 4079
NspI r_CATG'y
Cuts at: 1132 1632 2539 1132
Size: 500 907 2672
Fragments arranged by size:
2672 907 500
PciI A'CATG_T
Cuts at: 2535 2535
Size: 4079
PmeI GTTT'AAAC
Cuts at: 1078 1078
Size: 4079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 4047
PpuMI rG'GwC_Cy
Cuts at: 275 275
Size: 4079
PspOMI G'GGCC_C
Cuts at: 817 817
Size: 4079
PstI C_TGCA'G
Cuts at: 632 831 1126 1811 632
Size: 199 295 685 2900
Fragments arranged by size:
2900 685 295 199
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4079
PvuII CAG'CTG
Cuts at: 924 1504 1864 924
Size: 580 360 3139
Fragments arranged by size:
3139 580 360
RsrII CG'GwC_CG
Cuts at: 2274 2274
Size: 4079
SacI G_AGCT'C
Cuts at: 395 1093 395
Size: 698 3381
SalI G'TCGA_C
Cuts at: 1116 1116
Size: 4079
SapI GCTCTTCn'nnn_
Cuts at: 253 2102 2312 253
Size: 1849 210 2020
Fragments arranged by size:
2020 1849 210
SbfI CC_TGCA'GG
Cuts at: 632 1126 632
Size: 494 3585
ScaI AGT'ACT
Cuts at: 3596 3596
Size: 4079
SfcI C'TryA_G
Cuts at: 33 417 628 827 1122 1807 2800 2991 33
Size: 384 211 199 295 685 993 191 1121
Fragments arranged by size:
1121 993 685 384 295 211 199 191
SfoI GGC'GCC
Cuts at: 1511 1759 1511
Size: 248 3831
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
SmaI CCC'GGG
Cuts at: 1101 1101
Size: 4079
SmlI C'TyrA_G
Cuts at: 1027 1062 1234 2149 2641 2903 1027
Size: 35 172 915 492 262 2203
Fragments arranged by size:
2203 915 492 262 172 35
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4079
SphI G_CATG'C
Cuts at: 1132 1632 1132
Size: 500 3579
StyI C'CwwG_G
Cuts at: 107 270 605 857 943 1213 1567 107
Size: 163 335 252 86 270 354 2619
Fragments arranged by size:
2619 354 335 270 252 163 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1662 1795 2254 2463 1662
Size: 133 459 209 3278
Fragments arranged by size:
3278 459 209 133
TatI w'GTAC_w
Cuts at: 3594 3594
Size: 4079
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 4047
Tth111I GACn'n_nGTC
Cuts at: 1876 1876
Size: 4079
XbaI T'CTAG_A
Cuts at: 1110 1110
Size: 4079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1005 1574 1005
Size: 569 3510
XhoI C'TCGA_G
Cuts at: 1062 1062
Size: 4079
XmaI C'CCGG_G
Cuts at: 1099 1099
Size: 4079
XmnI GAAnn'nnTTC
Cuts at: 163 163
Size: 4079
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI