Restriction map for
pF1KE2499
(Circular) (Six-base) MAPSORT of: pF1KE2499.seq Check: 9165 from: 1 to: 16835
pF1KE2499 16835 bp
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2628 4683 5943 6507 6726 7002 8034 10845 13504
Size: 2055 1260 564 219 276 1032 2811 2659
Cuts at: 13504 2628
Size: 5959
Fragments arranged by size:
5959 2811 2659 2055 1260 1032 564 276
219
AatII G_ACGT'C
Cuts at: 8900 12580 8900
Size: 3680 13155
AccI GT'mk_AC
Cuts at: 8873 11273 13873 8873
Size: 2400 2600 11835
Fragments arranged by size:
11835 2600 2400
Acc65I G'GTAC_C
Cuts at: 710 13851 710
Size: 13141 3694
AclI AA'CG_TT
Cuts at: 16675 16675
Size: 16835
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 1048 1174 1354 1684 2209 2362 2590 2959
Size: 884 126 180 330 525 153 228 369
Cuts at: 2959 3604 5998 6559 6844 8317 9340 9568 10086
Size: 645 2394 561 285 1473 1023 228 518
Cuts at: 10086 10653 12157 12313 12571 13330 14660 15092 15839
Size: 567 1504 156 258 759 1330 432 747
Cuts at: 15839 16107 164
Size: 268 892
Fragments arranged by size:
2394 1504 1473 1330 1023 892 884 759
747 645 567 561 525 518 432 369
330 285 268 258 228 228 180 156
153 126
AfeI AGC'GCT
Cuts at: 3139 3715 3748 4681 4969 5086 6979 7326 7402
Size: 576 33 933 288 117 1893 347 76
Cuts at: 7402 7708 8797 9202 13174 13345 3139
Size: 306 1089 405 3972 171 6629
Fragments arranged by size:
6629 3972 1893 1089 933 576 405 347
306 288 171 117 76 33
AflIII A'CryG_T
Cuts at: 4986 14187 15291 16268 4986
Size: 9201 1104 977 5553
Fragments arranged by size:
9201 5553 1104 977
AgeI A'CCGG_T
Cuts at: 12106 13165 15223 12106
Size: 1059 2058 13718
Fragments arranged by size:
13718 2058 1059
AhdI GACnn_n'nnGTC
Cuts at: 933 11697 933
Size: 10764 6071
AleI CACnn'nnGTG
Cuts at: 377 2660 3240 8221 9073 10855 377
Size: 2283 580 4981 852 1782 6357
Fragments arranged by size:
6357 4981 2283 1782 852 580
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4546 4922 8066 8617 9145 10594 12328 13585 4546
Size: 376 3144 551 528 1449 1734 1257 7796
Fragments arranged by size:
7796 3144 1734 1449 1257 551 528 376
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1090 1091 1122 1123 1090
Size: 1 31 1 16802
Fragments arranged by size:
16802 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 142 720 1152 1770 2409 2823 3090 3093 3102
Size: 578 432 618 639 414 267 3 9
Cuts at: 3102 3948 4461 4620 4800 5313 5382 5445 5553
Size: 846 513 159 180 513 69 63 108
Cuts at: 5553 6393 6720 6750 6822 7257 7488 7641 7659
Size: 840 327 30 72 435 231 153 18
Cuts at: 7659 7776 7830 7914 7968 7989 8160 8349 8790
Size: 117 54 84 54 21 171 189 441
Cuts at: 8790 9186 9300 9546 9579 9996 10253 10407 10467
Size: 396 114 246 33 417 257 154 60
Cuts at: 10467 10767 11097 11298 13119 13308 13365 13461 14140
Size: 300 330 201 1821 189 57 96 679
Cuts at: 14140 15707 142
Size: 1567 1270
Fragments arranged by size:
1821 1567 1270 846 840 679 639 618
578 513 513 441 435 432 417 414
396 330 327 300 267 257 246 231
201 189 189 180 171 159 154 153
117 114 108 96 84 72 69 63
60 57 54 54 33 30 21 18
9 3
ApaI G_GGCC'C
Cuts at: 2686 3510 3600 4791 8470 9454 9699 9846 10447
Size: 824 90 1191 3679 984 245 147 601
Cuts at: 10447 10995 11453 12181 13024 13813 2686
Size: 548 458 728 843 789 5708
Fragments arranged by size:
5708 3679 1191 984 843 824 789 728
601 548 458 245 147 90
ApaLI G'TGCA_C
Cuts at: 2653 3113 3199 4625 8644 10621 15605 2653
Size: 460 86 1426 4019 1977 4984 3883
Fragments arranged by size:
4984 4019 3883 1977 1426 460 86
ApoI r'AATT_y
Cuts at: 1888 6703 13839 16188 16444 1888
Size: 4815 7136 2349 256 2279
Fragments arranged by size:
7136 4815 2349 2279 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 16835
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 16835
AvaI C'yCGr_G
Cuts at: 438 455 1732 1946 3031 3562 3793 6476 6569
Size: 17 1277 214 1085 531 231 2683 93
Cuts at: 6569 8470 8812 8891 9454 9566 9665 9839 10447
Size: 1901 342 79 563 112 99 174 608
Cuts at: 10447 10509 10789 10978 11167 11218 11452 12181 12625
Size: 62 280 189 189 51 234 729 444
Cuts at: 12625 12897 13679 13761 13785 13855 438
Size: 272 782 82 24 70 3418
Fragments arranged by size:
3418 2683 1901 1277 1085 782 729 608
563 531 444 342 280 272 234 231
214 189 189 174 112 99 93 82
79 70 62 51 24 17
BamHI G'GATC_C
Cuts at: 12940 13860 12940
Size: 920 15915
BanI G'GyrC_C
Cuts at: 521 710 1257 1577 1727 1951 2760 2837 3847
Size: 189 547 320 150 224 809 77 1010
Cuts at: 3847 4838 5296 6134 6254 9129 9253 9376 9705
Size: 991 458 838 120 2875 124 123 329
Cuts at: 9705 9782 11194 11777 11794 11980 12788 13474 13851
Size: 77 1412 583 17 186 808 686 377
Cuts at: 13851 14265 14513 14548 521
Size: 414 248 35 2808
Fragments arranged by size:
2875 2808 1412 1010 991 838 809 808
686 583 547 458 414 377 329 320
248 224 189 186 150 124 123 120
77 77 35 17
BanII G_rGCy'C
Cuts at: 2213 2686 3155 3510 3563 3600 3794 4474 4791
Size: 473 469 355 53 37 194 680 317
Cuts at: 4791 6576 6878 7374 7454 8234 8470 9176 9246
Size: 1785 302 496 80 780 236 706 70
Cuts at: 9246 9454 9699 9773 9846 9860 9956 10139 10167
Size: 208 245 74 73 14 96 183 28
Cuts at: 10167 10225 10447 10995 11260 11453 11708 12086 12181
Size: 58 222 548 265 193 255 378 95
Cuts at: 12181 13024 13388 13813 13849 14879 2213
Size: 843 364 425 36 1030 4169
Fragments arranged by size:
4169 1785 1030 843 780 706 680 548
496 473 469 425 378 364 355 317
302 265 255 245 236 222 208 194
193 183 96 95 80 74 73 70
58 53 37 36 28 14
BbeI G_GCGC'C
Cuts at: 1731 3851 4842 6138 6258 12792 13478 14269 14517
Size: 2120 991 1296 120 6534 686 791 248
Cuts at: 14517 1731
Size: 4049
Fragments arranged by size:
6534 4049 2120 1296 991 791 686 248
120
BbsI GAAGACnn'nnnn_
Cuts at: 398 1360 4957 5660 8252 11077 11994 12580 398
Size: 962 3597 703 2592 2825 917 586 4653
Fragments arranged by size:
4653 3597 2825 2592 962 917 703 586
BbvCI CC'TCA_GC
Cuts at: 125 920 5510 5938 6146 6458 7169 7328 7385
Size: 795 4590 428 208 312 711 159 57
Cuts at: 7385 7952 10156 10835 11135 11752 125
Size: 567 2204 679 300 617 5208
Fragments arranged by size:
5208 4590 2204 795 711 679 617 567
428 312 300 208 159 57
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2866 2900 3288 3322 4347 4381 7434 7468 8262
Size: 34 388 34 1025 34 3053 34 794
Cuts at: 8262 8296 9144 9178 2866
Size: 34 848 34 10523
Fragments arranged by size:
10523 3053 1025 848 794 388 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 14733 15494 16835 14733
Size: 761 1341 14733
Fragments arranged by size:
14733 1341 761
BclI T'GATC_A
Cuts at: 11042 12767 14355 11042
Size: 1725 1588 13522
Fragments arranged by size:
13522 1725 1588
BglI GCCn_nnn'nGGC
Cuts at: 1186 1865 2548 2788 3544 3574 4084 4504 4592
Size: 679 683 240 756 30 510 420 88
Cuts at: 4592 4768 4795 5053 5107 5458 5500 5593 6310
Size: 176 27 258 54 351 42 93 717
Cuts at: 6310 6451 6841 7054 8014 9449 9487 9703 10262
Size: 141 390 213 960 1435 38 216 559
Cuts at: 10262 10339 10429 10480 10931 11344 11599 13249 13303
Size: 77 90 51 451 413 255 1650 54
Cuts at: 13303 1186
Size: 4718
Fragments arranged by size:
4718 1650 1435 960 756 717 683 679
559 510 451 420 413 390 351 258
255 240 216 213 176 141 93 90
88 77 54 54 51 42 38 30
27
BglII A'GATC_T
Cuts at: 623 8543 13517 14350 16387 623
Size: 7920 4974 833 2037 1071
Fragments arranged by size:
7920 4974 2037 1071 833
BlpI GC'TnA_GC
Cuts at: 275 2713 3050 4241 4517 5018 6907 7334 7547
Size: 2438 337 1191 276 501 1889 427 213
Cuts at: 7547 9808 11480 13947 275
Size: 2261 1672 2467 3163
Fragments arranged by size:
3163 2467 2438 2261 1889 1672 1191 501
427 337 276 213
Bme1580I G_kGCm'C
Cuts at: 1262 1914 2657 2686 2744 2765 3117 3203 3249
Size: 652 743 29 58 21 352 86 46
Cuts at: 3249 3510 3600 3657 4629 4791 5031 5613 6639
Size: 261 90 57 972 162 240 582 1026
Cuts at: 6639 8470 8648 8663 9386 9454 9647 9699 9846
Size: 1831 178 15 723 68 193 52 147
Cuts at: 9846 10119 10447 10625 10995 11197 11453 12181 13024
Size: 273 328 178 370 202 256 728 843
Cuts at: 13024 13409 13564 13813 14460 14553 15609 1262
Size: 385 155 249 647 93 1056 2488
Fragments arranged by size:
2488 1831 1056 1026 972 843 743 728
723 652 647 582 385 370 352 328
273 261 256 249 240 202 193 178
178 162 155 147 93 90 86 68
58 57 52 46 29 21 15
BmgBI CAC'GTC
Cuts at: 909 990 1554 2895 3702 3777 8250 8670 10647
Size: 81 564 1341 807 75 4473 420 1977
Cuts at: 10647 13470 909
Size: 2823 4274
Fragments arranged by size:
4473 4274 2823 1977 1341 807 564 420
81 75
BmrI ACTGGGnnnn_n'
Cuts at: 3811 10161 12233 14047 14128 14463 16607 3811
Size: 6350 2072 1814 81 335 2144 4039
Fragments arranged by size:
6350 4039 2144 2072 1814 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 453 2178 3081 4758 5541 6897 9879 10239 15202
Size: 1725 903 1677 783 1356 2982 360 4963
Cuts at: 15202 453
Size: 2086
Fragments arranged by size:
4963 2982 2086 1725 1677 1356 903 783
360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 726 768 856 1098 1246 1342 1381 1759
Size: 278 42 88 242 148 96 39 378
Cuts at: 1759 2053 2139 2203 2488 2824 2995 3196 3409
Size: 294 86 64 285 336 171 201 213
Cuts at: 3409 3430 3463 3973 4024 4315 4822 4909 4984
Size: 21 33 510 51 291 507 87 75
Cuts at: 4984 5101 5290 5404 5575 5848 5926 6040 6046
Size: 117 189 114 171 273 78 114 6
Cuts at: 6046 6109 6232 6793 7312 7336 7510 7573 7756
Size: 63 123 561 519 24 174 63 183
Cuts at: 7756 7936 8011 8398 8626 8812 9169 9301 9757
Size: 180 75 387 228 186 357 132 456
Cuts at: 9757 10294 10375 10603 10789 11380 11668 12109 12337
Size: 537 81 228 186 591 288 441 228
Cuts at: 12337 12715 12960 13075 13144 13372 13600 448
Size: 378 245 115 69 228 228 3683
Fragments arranged by size:
3683 591 561 537 519 510 507 456
441 387 378 378 357 336 294 291
288 285 278 273 245 242 228 228
228 228 228 213 201 189 186 186
183 180 174 171 171 148 132 123
117 115 114 114 96 88 87 86
81 78 75 75 69 64 63 63
51 42 39 33 24 21 6
Bpu10I CC'TnA_GC
Cuts at: 125 920 3502 5510 5938 6146 6458 6575 7169
Size: 795 2582 2008 428 208 312 117 594
Cuts at: 7169 7328 7385 7952 10156 10835 11135 11752 11770
Size: 159 57 567 2204 679 300 617 18
Cuts at: 11770 125
Size: 5190
Fragments arranged by size:
5190 2582 2204 2008 795 679 617 594
567 428 312 300 208 159 117 57
18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 1549 3436 3508 3763 4087 5080 5716 5740
Size: 312 1887 72 255 324 993 636 24
Cuts at: 5740 6901 7108 7147 7327 7585 10165 11302 13498
Size: 1161 207 39 180 258 2580 1137 2196
Cuts at: 13498 14011 14890 15382 15680 1237
Size: 513 879 492 298 2392
Fragments arranged by size:
2580 2392 2196 1887 1161 1137 993 879
636 513 492 324 312 298 258 255
207 180 72 39 24
BsaI GGTCTCn'nnnn_
Cuts at: 1148 6772 9679 9877 10069 10493 10840 16539 1148
Size: 5624 2907 198 192 424 347 5699 1444
Fragments arranged by size:
5699 5624 2907 1444 424 347 198 192
BsaAI yAC'GTr
Cuts at: 1311 2850 4137 4989 8777 10754 11115 11727 14188
Size: 1539 1287 852 3788 1977 361 612 2461
Cuts at: 14188 1311
Size: 3958
Fragments arranged by size:
3958 3788 2461 1977 1539 1287 852 612
361
BsaBI GATnn'nnATC
Cuts at: 6 1132 9064 11047 12733 14349 16386 6
Size: 1126 7932 1983 1686 1616 2037 455
Fragments arranged by size:
7932 2037 1983 1686 1616 1126 455
BsaHI Gr'CG_yC
Cuts at: 968 1436 1728 3629 3848 3863 4839 6135 6255
Size: 468 292 1901 219 15 976 1296 120
Cuts at: 6255 7394 8897 9593 9719 11825 12011 12577 12789
Size: 1139 1503 696 126 2106 186 566 212
Cuts at: 12789 13190 13442 13475 13709 14266 14514 15216 16712
Size: 401 252 33 234 557 248 702 1496
Cuts at: 16712 968
Size: 1091
Fragments arranged by size:
2106 1901 1503 1496 1296 1139 1091 976
702 696 566 557 468 401 292 252
248 234 219 212 186 126 120 33
15
BsaWI w'CCGG_w
Cuts at: 7406 12106 13085 13165 14024 14545 15223 15497 15644
Size: 4700 979 80 859 521 678 274 147
Cuts at: 15644 16055 7406
Size: 411 8186
Fragments arranged by size:
8186 4700 979 859 678 521 411 274
147 80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 403 433 459 489 724 754 1279 1309 2062
Size: 30 26 30 235 30 525 30 753
Cuts at: 2062 2092 5314 5344 6265 6295 8866 8896 10081
Size: 30 3222 30 921 30 2571 30 1185
Cuts at: 10081 10111 10279 10309 10918 10948 11193 11223 12703
Size: 30 168 30 609 30 245 30 1480
Cuts at: 12703 12733 12766 12796 13048 13078 13345 13375 403
Size: 30 33 30 252 30 267 30 3863
Fragments arranged by size:
3863 3222 2571 1480 1185 921 753 609
525 267 252 245 235 168 33 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1138 1429 1801 1998 2110 2113 2116 2962 3508
Size: 291 372 197 112 3 3 846 546
Cuts at: 3508 3529 3970 4000 4237 4240 4243 4273 4399
Size: 21 441 30 237 3 3 30 126
Cuts at: 4399 4417 4462 4555 4558 4651 4684 4819 4840
Size: 18 45 93 3 93 33 135 21
Cuts at: 4840 5026 5077 5149 5203 5242 5401 5404 5527
Size: 186 51 72 54 39 159 3 123
Cuts at: 5527 5530 5605 5845 5878 5974 5977 6136 6139
Size: 3 75 240 33 96 3 159 3
Cuts at: 6139 6163 6193 6196 6259 6475 6484 6568 6790
Size: 24 30 3 63 216 9 84 222
Cuts at: 6790 6823 6883 6886 6916 7006 7036 7096 7279
Size: 33 60 3 30 90 30 60 183
Cuts at: 7279 7402 7435 7624 7798 7861 7891 7915 7954
Size: 123 33 189 174 63 30 24 39
Cuts at: 7954 7969 8008 8041 8203 8713 9094 9298 9697
Size: 15 39 33 162 510 381 204 399
Cuts at: 9697 9868 10081 10690 10882 10885 10996 11584 11887
Size: 171 213 609 192 3 111 588 303
Cuts at: 11887 11959 12166 12370 12424 12490 12729 12732 12739
Size: 72 207 204 54 66 239 3 7
Cuts at: 12739 12921 12936 12939 13027 13570 13746 13749 1138
Size: 182 15 3 88 543 176 3 4224
Fragments arranged by size:
4224 846 609 588 546 543 510 441
399 381 372 303 291 240 239 237
222 216 213 207 204 204 197 192
189 186 183 182 176 174 171 162
159 159 135 126 123 123 112 111
96 93 93 90 88 84 75 72
72 66 63 63 60 60 54 54
51 45 39 39 39 33 33 33
33 33 30 30 30 30 30 30
24 24 21 21 18 15 15 9
7 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 175 244 270 1834 1851 2221 2583 2810 3248
Size: 69 26 1564 17 370 362 227 438
Cuts at: 3248 3294 3399 3734 5876 6030 7397 7769 8725
Size: 46 105 335 2142 154 1367 372 956
Cuts at: 8725 9596 9625 9739 10218 10702 10994 11594 11707
Size: 871 29 114 479 484 292 600 113
Cuts at: 11707 12983 13159 14260 15018 15595 16091 175
Size: 1276 176 1101 758 577 496 919
Fragments arranged by size:
2142 1564 1367 1276 1101 956 919 871
758 600 577 496 484 479 438 372
370 362 335 292 227 176 154 114
113 105 69 46 29 26 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 598 1012 1291 1576 1603 1741 1855 2584
Size: 261 414 279 285 27 138 114 729
Cuts at: 2584 2672 2917 2950 2965 3854 4717 4928 6178
Size: 88 245 33 15 889 863 211 1250
Cuts at: 6178 6382 6502 6913 7165 7423 7501 7645 8461
Size: 204 120 411 252 258 78 144 816
Cuts at: 8461 11443 13122 13378 337
Size: 2982 1679 256 3794
Fragments arranged by size:
3794 2982 1679 1250 889 863 816 729
414 411 285 279 261 258 256 252
245 211 204 144 138 120 114 88
78 33 27 15
BsiEI CG_ry'CG
Cuts at: 104 759 1088 2127 5571 6002 6176 8498 9168
Size: 655 329 1039 3444 431 174 2322 670
Cuts at: 9168 11022 11466 12209 12777 12834 13298 13773 14377
Size: 1854 444 743 568 57 464 475 604
Cuts at: 14377 14423 15631 104
Size: 46 1208 1308
Fragments arranged by size:
3444 2322 1854 1308 1208 1039 743 670
655 604 568 475 464 444 431 329
174 57 46
BsiHKAI G_wGCw'C
Cuts at: 2213 2657 2714 3117 3155 3203 3503 3563 3794
Size: 444 57 403 38 48 300 60 231
Cuts at: 3794 4474 4629 6878 7454 8120 8234 8591 8648
Size: 680 155 2249 576 666 114 357 57
Cuts at: 8648 9176 9773 9860 9956 10139 10625 12086 13849
Size: 528 597 87 96 183 486 1461 1763
Cuts at: 13849 14627 14817 15609 2213
Size: 778 190 792 3439
Fragments arranged by size:
3439 2249 1763 1461 792 778 680 666
597 576 528 486 444 403 357 300
231 190 183 155 114 96 87 60
57 57 48 38
BsiWI C'GTAC_G
Cuts at: 4147 4147
Size: 16835
BsmI GAATG_Cn'
Cuts at: 4439 4439
Size: 16835
BsmBI CGTCTCn'nnnn_
Cuts at: 746 957 3181 4885 5344 7261 7642 8809 9206
Size: 211 2224 1704 459 1917 381 1167 397
Cuts at: 9206 9534 10786 12622 12978 13009 13042 746
Size: 328 1252 1836 356 31 33 4539
Fragments arranged by size:
4539 2224 1917 1836 1704 1252 1167 459
397 381 356 328 211 33 31
Bsp1286I G_dGCh'C
Cuts at: 1262 1914 2213 2657 2686 2714 2744 2765 3117
Size: 652 299 444 29 28 30 21 352
Cuts at: 3117 3155 3203 3249 3503 3510 3563 3600 3657
Size: 38 48 46 254 7 53 37 57
Cuts at: 3657 3794 4474 4629 4791 5031 5613 6576 6639
Size: 137 680 155 162 240 582 963 63
Cuts at: 6639 6878 7374 7454 8120 8234 8470 8591 8648
Size: 239 496 80 666 114 236 121 57
Cuts at: 8648 8663 9176 9246 9386 9454 9647 9699 9773
Size: 15 513 70 140 68 193 52 74
Cuts at: 9773 9846 9860 9956 10119 10139 10167 10225 10447
Size: 73 14 96 163 20 28 58 222
Cuts at: 10447 10625 10995 11197 11260 11453 11708 12086 12181
Size: 178 370 202 63 193 255 378 95
Cuts at: 12181 13024 13388 13409 13564 13813 13849 14460 14553
Size: 843 364 21 155 249 36 611 93
Cuts at: 14553 14627 14817 14879 15609 1262
Size: 74 190 62 730 2488
Fragments arranged by size:
2488 963 843 730 680 666 652 611
582 513 496 444 378 370 364 352
299 255 254 249 240 239 236 222
202 193 193 190 178 163 162 155
155 140 137 121 114 96 95 93
80 74 74 73 70 68 63 63
62 58 57 57 53 52 48 46
38 37 36 30 29 28 28 21
21 20 15 14 7
BspEI T'CCGG_A
Cuts at: 7406 16055 7406
Size: 8649 8186
BspHI T'CATG_A
Cuts at: 9462 16011 9462
Size: 6549 10286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1094 1769 1778 1838 1991 2483 2628 3405 4151
Size: 675 9 60 153 492 145 777 746
Cuts at: 4151 4683 4863 5943 6507 6726 6806 7002 8034
Size: 532 180 1080 564 219 80 196 1032
Cuts at: 8034 8270 8789 9017 9338 10845 11189 11285 11880
Size: 236 519 228 321 1507 344 96 595
Cuts at: 11880 12500 13504 13885 14099 14401 14782 1094
Size: 620 1004 381 214 302 381 3147
Fragments arranged by size:
3147 1507 1080 1032 1004 777 746 675
620 595 564 532 519 492 381 381
344 321 302 236 228 219 214 196
180 153 145 96 80 60 9
BsrBI CCG'CTC
Cuts at: 50 279 306 870 1234 1326 1908 2909 3696
Size: 229 27 564 364 92 582 1001 787
Cuts at: 3696 3726 4269 4350 4836 5022 5052 5073 5685
Size: 30 543 81 486 186 30 21 612
Cuts at: 5685 5910 6129 6225 6271 6534 7302 9822 10773
Size: 225 219 96 46 263 768 2520 951
Cuts at: 10773 11409 11484 12570 12782 12836 12920 15127 15181
Size: 636 75 1086 212 54 84 2207 54
Cuts at: 15181 16665 16830 50
Size: 1484 165 55
Fragments arranged by size:
2520 2207 1484 1086 1001 951 787 768
636 612 582 564 543 486 364 263
229 225 219 212 186 165 96 92
84 81 75 55 54 54 46 30
30 27 21
BsrDI GCAATG_nn'
Cuts at: 14747 14747
Size: 16835
BsrFI r'CCGG_y
Cuts at: 224 782 821 2228 3038 3231 3543 4071 4526
Size: 558 39 1407 810 193 312 528 455
Cuts at: 4526 5566 5570 8170 8423 8678 9098 9373 9753
Size: 1040 4 2600 253 255 420 275 380
Cuts at: 9753 9785 10261 10489 11017 11494 11605 11797 12106
Size: 32 476 228 528 477 111 192 309
Cuts at: 12106 12365 12859 12907 13165 13336 13369 13660 13672
Size: 259 494 48 258 171 33 291 12
Cuts at: 13672 13684 13714 14833 15014 15223 224
Size: 12 30 1119 181 209 1836
Fragments arranged by size:
2600 1836 1407 1119 1040 810 558 528
528 494 477 476 455 420 380 312
309 291 275 259 258 255 253 228
209 193 192 181 171 111 48 39
33 32 30 12 12 4
BsrGI T'GTAC_A
Cuts at: 10859 10859
Size: 16835
BssHII G'CGCG_C
Cuts at: 4771 5056 8502 12213 4771
Size: 285 3446 3711 9393
Fragments arranged by size:
9393 3711 3446 285
BssSI C'ACGA_G
Cuts at: 1621 3511 8764 10417 10456 11443 15106 15464 1621
Size: 1890 5253 1653 39 987 3663 358 2992
Fragments arranged by size:
5253 3663 2992 1890 1653 987 358 39
BstAPI GCAn_nnn'nTGC
Cuts at: 1719 1770 2409 2844 3093 3207 3759 3948 4377
Size: 51 639 435 249 114 552 189 429
Cuts at: 4377 4461 4620 4624 6393 6411 6720 7143 7623
Size: 84 159 4 1769 18 309 423 480
Cuts at: 7623 7641 7659 9436 9579 10253 11987 13119 14212
Size: 18 18 1777 143 674 1734 1132 1093
Cuts at: 14212 1719
Size: 4342
Fragments arranged by size:
4342 1777 1769 1734 1132 1093 674 639
552 480 435 429 423 309 249 189
159 143 114 84 51 18 18 18
4
BstBI TT'CG_AA
Cuts at: 15196 15196
Size: 16835
BstEII G'GTnAC_C
Cuts at: 7470 8952 10023 11025 11490 11949 7470
Size: 1482 1071 1002 465 459 12356
Fragments arranged by size:
12356 1482 1071 1002 465 459
BstXI CCAn_nnnn'nTGG
Cuts at: 643 2221 3441 3465 3900 643
Size: 1578 1220 24 435 13578
Fragments arranged by size:
13578 1578 1220 435 24
BstYI r'GATC_y
Cuts at: 1 623 1133 3884 4484 4547 5966 7493 8543
Size: 622 510 2751 600 63 1419 1527 1050
Cuts at: 8543 12422 12940 13517 13860 14327 14350 14685 14931
Size: 3879 518 577 343 467 23 335 246
Cuts at: 14931 15943 16029 16041 16387 1
Size: 1012 86 12 346 449
Fragments arranged by size:
3879 2751 1527 1419 1050 1012 622 600
577 518 510 467 449 346 343 335
246 86 63 23 12
Bsu36I CC'TnA_GG
Cuts at: 902 2023 8394 12353 902
Size: 1121 6371 3959 5384
Fragments arranged by size:
6371 5384 3959 1121
BtgI C'CryG_G
Cuts at: 2261 3474 3969 5318 6836 8096 8219 8621 8663
Size: 1213 495 1349 1518 1260 123 402 42
Cuts at: 8663 9350 9419 9605 9914 10028 10598 10926 11261
Size: 687 69 186 309 114 570 328 335
Cuts at: 11261 11844 11867 11906 12300 12332 12374 13025 13695
Size: 583 23 39 394 32 42 651 670
Cuts at: 13695 13823 2261
Size: 128 5273
Fragments arranged by size:
5273 1518 1349 1260 1213 687 670 651
583 570 495 402 394 335 328 309
186 128 123 114 69 42 42 39
32 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 279 416 546 738 3240 3341 7527 9249 11976
Size: 137 130 192 2502 101 4186 1722 2727
Cuts at: 11976 12798 14785 14966 15129 279
Size: 822 1987 181 163 1985
Fragments arranged by size:
4186 2727 2502 1987 1985 1722 822 192
181 163 137 130 101
BtsI GCAGTG_nn'
Cuts at: 171 190 2302 2419 4618 4911 6757 8033 9437
Size: 19 2112 117 2199 293 1846 1276 1404
Cuts at: 9437 9974 10254 14078 171
Size: 537 280 3824 2928
Fragments arranged by size:
3824 2928 2199 2112 1846 1404 1276 537
293 280 117 19
ClaI AT'CG_AT
Cuts at: 12803 12803
Size: 16835
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1676 1711 12891 12926 1676
Size: 35 11180 35 5585
Fragments arranged by size:
11180 5585 35 35
DraI TTT'AAA
Cuts at: 13834 13834
Size: 16835
DraIII CAC_nnn'GTG
Cuts at: 338 1803 1918 2834 4624 8658 9642 10635 338
Size: 1465 115 916 1790 4034 984 993 6538
Fragments arranged by size:
6538 4034 1790 1465 993 984 916 115
DrdI GACnn_nn'nnGTC
Cuts at: 127 913 12754 13290 14541 15399 127
Size: 786 11841 536 1251 858 1563
Fragments arranged by size:
11841 1563 1251 858 786 536
EaeI y'GGCC_r
Cuts at: 405 665 756 1085 1313 2540 2618 2921 3545
Size: 260 91 329 228 1227 78 303 624
Cuts at: 3545 3902 4085 4274 4313 5315 5459 5492 5568
Size: 357 183 189 39 1002 144 33 76
Cuts at: 5568 5585 5645 5705 5999 6173 7355 7760 7835
Size: 17 60 60 294 174 1182 405 75
Cuts at: 7835 8073 8495 8535 9347 9755 10367 11019 11600
Size: 238 422 40 812 408 612 652 581
Cuts at: 11600 11799 12101 12206 13295 13338 13770 13826 14420
Size: 199 302 105 1089 43 432 56 594
Cuts at: 14420 14594 14985 15012 16357 405
Size: 174 391 27 1345 883
Fragments arranged by size:
1345 1227 1182 1089 1002 883 812 652
624 612 594 581 432 422 408 405
391 357 329 303 302 294 260 238
228 199 189 183 174 174 144 105
91 78 76 75 60 60 56 43
40 39 33 27 17
EagI C'GGCC_G
Cuts at: 756 1085 5568 5999 6173 8495 11019 12206 13295
Size: 329 4483 431 174 2322 2524 1187 1089
Cuts at: 13295 13770 14420 756
Size: 475 650 3171
Fragments arranged by size:
4483 3171 2524 2322 1187 1089 650 475
431 329 174
EarI CTCTTCn'nnn_
Cuts at: 769 1549 2854 3116 5146 5615 6157 6332 6518
Size: 780 1305 262 2030 469 542 175 186
Cuts at: 6518 6883 7750 8557 10534 10886 11539 12268 12406
Size: 365 867 807 1977 352 653 729 138
Cuts at: 12406 13746 14858 15068 769
Size: 1340 1112 210 2536
Fragments arranged by size:
2536 2030 1977 1340 1305 1112 867 807
780 729 653 542 469 365 352 262
210 186 175 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 880 1537 2439 4360 4495 4648 4657 4741 4837
Size: 657 902 1921 135 153 9 84 96
Cuts at: 4837 4846 4930 5146 5209 5239 5305 5632 5695
Size: 9 84 216 63 30 66 327 63
Cuts at: 5695 5758 5785 5812 6028 6133 6349 6451 6481
Size: 63 27 27 216 105 216 102 30
Cuts at: 6481 6502 6658 6844 7033 7189 7276 7432 8154
Size: 21 156 186 189 156 87 156 722
Cuts at: 8154 8587 8734 10564 10711 10840 11074 11220 11239
Size: 433 147 1830 147 129 234 146 19
Cuts at: 11239 12052 12298 15043 15353 15499 16645 880
Size: 813 246 2745 310 146 1146 1070
Fragments arranged by size:
2745 1921 1830 1146 1070 902 813 722
657 433 327 310 246 234 216 216
216 189 186 156 156 156 153 147
147 146 146 135 129 105 102 96
87 84 84 66 63 63 63 30
30 27 27 21 19 9 9
EcoICRI GAG'CTC
Cuts at: 2211 3153 3561 3792 4472 6876 7452 8232 9174
Size: 942 408 231 680 2404 576 780 942
Cuts at: 9174 9771 9858 9954 10137 12084 13847 2211
Size: 597 87 96 183 1947 1763 5199
Fragments arranged by size:
5199 2404 1947 1763 942 942 780 680
597 576 408 231 183 96 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 448 726 768 856 1048 1098 1174 1246
Size: 284 278 42 88 192 50 76 72
Cuts at: 1246 1342 1354 1381 1684 1759 2053 2139 2203
Size: 96 12 27 303 75 294 86 64
Cuts at: 2203 2209 2362 2488 2590 2824 2959 2995 3196
Size: 6 153 126 102 234 135 36 201
Cuts at: 3196 3409 3430 3463 3604 3973 4024 4315 4822
Size: 213 21 33 141 369 51 291 507
Cuts at: 4822 4909 4984 5101 5290 5404 5575 5848 5926
Size: 87 75 117 189 114 171 273 78
Cuts at: 5926 5998 6040 6046 6109 6232 6559 6793 6844
Size: 72 42 6 63 123 327 234 51
Cuts at: 6844 7312 7336 7510 7573 7756 7936 8011 8317
Size: 468 24 174 63 183 180 75 306
Cuts at: 8317 8398 8626 8812 9169 9301 9340 9568 9757
Size: 81 228 186 357 132 39 228 189
Cuts at: 9757 10086 10294 10375 10603 10653 10789 11380 11668
Size: 329 208 81 228 50 136 591 288
Cuts at: 11668 12109 12157 12313 12337 12571 12715 12960 13075
Size: 441 48 156 24 234 144 245 115
Cuts at: 13075 13144 13330 13372 13600 14660 15092 15839 16107
Size: 69 186 42 228 1060 432 747 268
Cuts at: 16107 164
Size: 892
Fragments arranged by size:
1060 892 747 591 507 468 441 432
369 357 329 327 306 303 294 291
288 284 278 273 268 245 234 234
234 228 228 228 228 213 208 201
192 189 189 186 186 183 180 174
171 156 153 144 141 136 135 132
126 123 117 115 114 102 96 88
87 86 81 81 78 76 75 75
75 72 72 69 64 63 63 51
51 50 50 48 42 42 42 39
36 33 27 24 24 21 12 6
6
EcoNI CCTnn'n_nnAGG
Cuts at: 358 427 847 2479 3106 3952 4846 4978 5161
Size: 69 420 1632 627 846 894 132 183
Cuts at: 5161 6988 7534 8377 8605 9538 9550 9679 10084
Size: 1827 546 843 228 933 12 129 405
Cuts at: 10084 10354 10531 10582 10951 11146 11209 11677 12265
Size: 270 177 51 369 195 63 468 588
Cuts at: 12265 12316 13312 13414 358
Size: 51 996 102 3779
Fragments arranged by size:
3779 1827 1632 996 933 894 846 843
627 588 546 468 420 405 369 270
228 195 183 177 132 129 102 69
63 51 51 12
EcoO109I rG'GnC_Cy
Cuts at: 432 717 873 1767 2027 2472 2683 2820 3506
Size: 285 156 894 260 445 211 137 686
Cuts at: 3506 3945 4788 4797 7086 7656 8467 8523 8571
Size: 439 843 9 2289 570 811 56 48
Cuts at: 8571 8808 9006 9114 9451 9507 9543 9555 9576
Size: 237 198 108 337 56 36 12 21
Cuts at: 9576 9695 9696 9909 10152 10206 10320 10444 10548
Size: 119 1 213 243 54 114 124 104
Cuts at: 10548 10785 11139 11439 11449 11574 11928 12178 12234
Size: 237 354 300 10 125 354 250 56
Cuts at: 12234 12282 12669 12980 13021 13203 13269 13305 13419
Size: 48 387 311 41 182 66 36 114
Cuts at: 13419 13790 13809 13810 13974 432
Size: 371 19 1 164 3293
Fragments arranged by size:
3293 2289 894 843 811 686 570 445
439 387 371 354 354 337 311 300
285 260 250 243 237 237 213 211
198 182 164 156 137 125 124 119
114 114 108 104 66 56 56 56
54 48 48 41 36 36 21 19
12 10 9 1 1
EcoRI G'AATT_C
Cuts at: 1888 13839 1888
Size: 11951 4884
EcoRV GAT'ATC
Cuts at: 633 633
Size: 16835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 345 6003 8763 10740 11856 12474 14854 345
Size: 5658 2760 1977 1116 618 2380 2326
Fragments arranged by size:
5658 2760 2380 2326 1977 1116 618
FseI GG_CCGG'CC
Cuts at: 5570 11021 13340 5570
Size: 5451 2319 9065
Fragments arranged by size:
9065 5451 2319
FspI TGC'GCA
Cuts at: 4534 5884 6067 6604 7205 9121 11719 12598 14616
Size: 1350 183 537 601 1916 2598 879 2018
Cuts at: 14616 4534
Size: 6753
Fragments arranged by size:
6753 2598 2018 1916 1350 879 601 537
183
HaeII r_GCGC'y
Cuts at: 245 1629 1731 1814 2170 2958 3141 3717 3750
Size: 1384 102 83 356 788 183 576 33
Cuts at: 3750 3851 3969 4587 4683 4842 4971 5088 5113
Size: 101 118 618 96 159 129 117 25
Cuts at: 5113 5313 5784 5871 5977 6138 6211 6258 6981
Size: 200 471 87 106 161 73 47 723
Cuts at: 6981 7328 7341 7404 7530 7710 8799 9204 9410
Size: 347 13 63 126 180 1089 405 206
Cuts at: 9410 10407 11751 11914 12606 12792 13176 13347 13478
Size: 997 1344 163 692 186 384 171 131
Cuts at: 13478 14269 14517 15539 16518 245
Size: 791 248 1022 979 562
Fragments arranged by size:
1384 1344 1089 1022 997 979 791 788
723 692 618 576 562 471 405 384
356 347 248 206 200 186 183 180
171 163 161 159 131 129 126 118
117 106 102 101 96 87 83 73
63 47 33 25 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 919 951 5503 5535 6265 6297 10081 10113 10279
Size: 32 4552 32 730 32 3784 32 166
Cuts at: 10279 10311 10492 10524 11148 11180 11212 11244 11683
Size: 32 181 32 624 32 32 32 439
Cuts at: 11683 11715 12226 12258 12599 12631 12766 12798 12880
Size: 32 511 32 341 32 135 32 82
Cuts at: 12880 12912 13048 13080 14677 14707 14709 14739 919
Size: 32 136 32 1597 30 2 30 3015
Fragments arranged by size:
4552 3784 3015 1597 730 624 511 439
341 181 166 136 135 82 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 345 8439 13197 13874 345
Size: 8094 4758 677 3306
Fragments arranged by size:
8094 4758 3306 677
HindIII A'AGCT_T
Cuts at: 7668 7668
Size: 16835
KasI G'GCGC_C
Cuts at: 1727 3847 4838 6134 6254 12788 13474 14265 14513
Size: 2120 991 1296 120 6534 686 791 248
Cuts at: 14513 1727
Size: 4049
Fragments arranged by size:
6534 4049 2120 1296 991 791 686 248
120
KpnI G_GTAC'C
Cuts at: 714 13855 714
Size: 13141 3694
MluI A'CGCG_T
Cuts at: 16268 16268
Size: 16835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 632 1430 4550 7599 9341 9365 9569 10946 11801
Size: 798 3120 3049 1742 24 204 1377 855
Cuts at: 11801 15506 15690 632
Size: 3705 184 1777
Fragments arranged by size:
3705 3120 3049 1777 1742 1377 855 798
204 184 24
MscI TGG'CCA
Cuts at: 407 1315 2542 2923 5461 5494 7762 7837 8537
Size: 908 1227 381 2538 33 2268 75 700
Cuts at: 8537 11602 14596 407
Size: 3065 2994 2646
Fragments arranged by size:
3065 2994 2646 2538 2268 1227 908 700
381 75 33
MslI CAynn'nnrTG
Cuts at: 377 805 2137 2660 3240 8221 9070 9073 9349
Size: 428 1332 523 580 4981 849 3 276
Cuts at: 9349 10855 14824 14951 377
Size: 1506 3969 127 2261
Fragments arranged by size:
4981 3969 2261 1506 1332 849 580 523
428 276 127 3
MspA1I CmG'CkG
Cuts at: 219 233 274 746 1023 1278 1422 1488 1851
Size: 14 41 472 277 255 144 66 363
Cuts at: 1851 2316 2508 2568 2676 3090 3174 3219 3476
Size: 465 192 60 108 414 84 45 257
Cuts at: 3476 3684 3738 3813 3900 3930 4044 4251 4305
Size: 208 54 75 87 30 114 207 54
Cuts at: 4305 4311 4461 4785 4962 5004 5247 5280 5286
Size: 6 150 324 177 42 243 33 6
Cuts at: 5286 5320 5373 5379 5637 5643 5670 5817 5880
Size: 34 53 6 258 6 27 147 63
Cuts at: 5880 5946 6004 6105 6111 6198 6294 6300 6423
Size: 66 58 101 6 87 96 6 123
Cuts at: 6423 6676 6771 6819 7074 7101 7257 7284 7308
Size: 253 95 48 255 27 156 27 24
Cuts at: 7308 7314 7638 7701 7776 7794 7827 7914 7926
Size: 6 324 63 75 18 33 87 12
Cuts at: 7926 7980 8098 8199 8346 9000 9186 9600 9780
Size: 54 118 101 147 654 186 414 180
Cuts at: 9780 9798 9987 10106 10464 10764 10989 10998 11598
Size: 18 189 119 358 300 225 9 600
Cuts at: 11598 11697 11766 11805 11919 11987 12498 12549 12987
Size: 99 69 39 114 68 511 51 438
Cuts at: 12987 13116 13631 13648 13697 13946 14260 14620 15633
Size: 129 515 17 49 249 314 360 1013
Cuts at: 15633 15878 219
Size: 245 1176
Fragments arranged by size:
1176 1013 654 600 515 511 472 465
438 414 414 363 360 358 324 324
314 300 277 258 257 255 255 253
249 245 243 225 208 207 192 189
186 180 177 156 150 147 147 144
129 123 119 118 114 114 108 101
101 99 96 95 87 87 87 84
75 75 69 68 66 66 63 63
60 58 54 54 54 53 51 49
48 45 42 41 39 34 33 33
30 27 27 27 24 18 18 17
14 12 9 6 6 6 6 6
6 6
NaeI GCC'GGC
Cuts at: 226 3233 3545 4528 5568 8172 9100 9375 9755
Size: 3007 312 983 1040 2604 928 275 380
Cuts at: 9755 9787 10263 11019 12861 13338 13686 15016 226
Size: 32 476 756 1842 477 348 1330 2045
Fragments arranged by size:
3007 2604 2045 1842 1330 1040 983 928
756 477 476 380 348 312 275 32
NarI GG'CG_CC
Cuts at: 1728 3848 4839 6135 6255 12789 13475 14266 14514
Size: 2120 991 1296 120 6534 686 791 248
Cuts at: 14514 1728
Size: 4049
Fragments arranged by size:
6534 4049 2120 1296 991 791 686 248
120
NcoI C'CATG_G
Cuts at: 11867 12300 11867
Size: 433 16402
NgoMIV G'CCGG_C
Cuts at: 224 3231 3543 4526 5566 8170 9098 9373 9753
Size: 3007 312 983 1040 2604 928 275 380
Cuts at: 9753 9785 10261 11017 12859 13336 13684 15014 224
Size: 32 476 756 1842 477 348 1330 2045
Fragments arranged by size:
3007 2604 2045 1842 1330 1040 983 928
756 477 476 380 348 312 275 32
NotI GC'GGCC_GC
Cuts at: 5999 5999
Size: 16835
NspI r_CATG'y
Cuts at: 4380 12894 13888 14388 15295 4380
Size: 8514 994 500 907 5920
Fragments arranged by size:
8514 5920 994 907 500
PasI CC'CwG_GG
Cuts at: 181 13803 181
Size: 13622 3213
PciI A'CATG_T
Cuts at: 15291 15291
Size: 16835
PflMI CCAn_nnn'nTGG
Cuts at: 1224 1278 2679 2823 11001 1224
Size: 54 1401 144 8178 7058
Fragments arranged by size:
8178 7058 1401 144 54
PfoI T'CCnGG_A
Cuts at: 263 893 1319 3554 4127 7748 7988 8582 11186
Size: 630 426 2235 573 3621 240 594 2604
Cuts at: 11186 11972 12086 12976 13037 13349 263
Size: 786 114 890 61 312 3749
Fragments arranged by size:
3749 3621 2604 2235 890 786 630 594
573 426 312 240 114 61
PmeI GTTT'AAAC
Cuts at: 13834 13834
Size: 16835
PmlI CAC'GTG
Cuts at: 1311 2850 4989 1311
Size: 1539 2139 13157
Fragments arranged by size:
13157 2139 1539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 11128 11160 15974 16006 11128
Size: 32 4814 32 11957
Fragments arranged by size:
11957 4814 32 32
PpuMI rG'GwC_Cy
Cuts at: 432 873 1767 2027 2472 7086 8523 8808 9006
Size: 441 894 260 445 4614 1437 285 198
Cuts at: 9006 9507 9909 10152 10206 10785 11139 12234 12980
Size: 501 402 243 54 579 354 1095 746
Cuts at: 12980 13269 13790 432
Size: 289 521 3477
Fragments arranged by size:
4614 3477 1437 1095 894 746 579 521
501 445 441 402 354 289 285 260
243 198 54
PshAI GACnn'nnGTC
Cuts at: 10198 10198
Size: 16835
PspOMI G'GGCC_C
Cuts at: 2682 3506 3596 4787 8466 9450 9695 9842 10443
Size: 824 90 1191 3679 984 245 147 601
Cuts at: 10443 10991 11449 12177 13020 13809 2682
Size: 548 458 728 843 789 5708
Fragments arranged by size:
5708 3679 1191 984 843 824 789 728
601 548 458 245 147 90
PspXI vC'TCGA_Gb
Cuts at: 3562 3793 3562
Size: 231 16604
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 802 834 2341 2373 4177 4209 8761 8793 10738
Size: 32 1507 32 1804 32 4552 32 1945
Cuts at: 10738 10770 12472 12504 802
Size: 32 1702 32 5133
Fragments arranged by size:
5133 4552 1945 1804 1702 1507 32 32
32 32 32 32
PstI C_TGCA'G
Cuts at: 491 1022 1394 1448 1473 1715 1766 1988 2291
Size: 531 372 54 25 242 51 222 303
Cuts at: 2291 2414 2483 3073 3098 3173 3680 3860 3935
Size: 123 69 590 25 75 507 180 75
Cuts at: 3935 4370 4457 4568 4616 4796 4820 4925 4979
Size: 435 87 111 48 180 24 105 54
Cuts at: 4979 5129 5408 5424 6299 6332 6398 6594 6770
Size: 150 279 16 875 33 66 196 176
Cuts at: 6770 6818 6989 7187 7229 7598 7646 7664 7910
Size: 48 171 198 42 369 48 18 246
Cuts at: 7910 7985 8165 8198 8267 8339 8786 9191 9875
Size: 75 180 33 69 72 447 405 684
Cuts at: 9875 10244 11918 12497 13427 13882 14567 491
Size: 369 1674 579 930 455 685 2759
Fragments arranged by size:
2759 1674 930 875 685 684 590 579
531 507 455 447 435 405 372 369
369 303 279 246 242 222 198 196
180 180 180 176 171 150 123 111
105 87 75 75 75 72 69 69
66 54 54 51 48 48 48 42
33 33 25 25 24 18 16
PvuI CG_AT'CG
Cuts at: 104 104
Size: 16835
PvuII CAG'CTG
Cuts at: 274 746 1023 1422 1851 2316 2508 2568 2676
Size: 472 277 399 429 465 192 60 108
Cuts at: 2676 3090 3174 3738 3813 3930 4044 4311 4461
Size: 414 84 564 75 117 114 267 150
Cuts at: 4461 4962 5004 5286 5379 5643 5880 6111 6294
Size: 501 42 282 93 264 237 231 183
Cuts at: 6294 6300 6423 6676 6771 7074 7257 7314 7638
Size: 6 123 253 95 303 183 57 324
Cuts at: 7638 7776 7914 7980 8346 9186 9600 9798 10464
Size: 138 138 66 366 840 414 198 666
Cuts at: 10464 10764 10998 11598 11697 11766 11805 11919 11987
Size: 300 234 600 99 69 39 114 68
Cuts at: 11987 12498 12987 14260 14620 274
Size: 511 489 1273 360 2489
Fragments arranged by size:
2489 1273 840 666 600 564 511 501
489 472 465 429 414 414 399 366
360 324 303 300 282 277 267 264
253 237 234 231 198 192 183 183
150 138 138 123 117 114 114 108
99 95 93 84 75 69 68 66
60 57 42 39 6
RsrII CG'GwC_CG
Cuts at: 15030 15030
Size: 16835
SacI G_AGCT'C
Cuts at: 2213 3155 3563 3794 4474 6878 7454 8234 9176
Size: 942 408 231 680 2404 576 780 942
Cuts at: 9176 9773 9860 9956 10139 12086 13849 2213
Size: 597 87 96 183 1947 1763 5199
Fragments arranged by size:
5199 2404 1947 1763 942 942 780 680
597 576 408 231 183 96 87
SacII CC_GC'GG
Cuts at: 3477 5321 8099 13698 3477
Size: 1844 2778 5599 6614
Fragments arranged by size:
6614 5599 2778 1844
SalI G'TCGA_C
Cuts at: 13872 13872
Size: 16835
SanDI GG'GwC_CC
Cuts at: 9507 9507
Size: 16835
SapI GCTCTTCn'nnn_
Cuts at: 1549 2854 5146 6157 6883 7750 11539 14858 15068
Size: 1305 2292 1011 726 867 3789 3319 210
Cuts at: 15068 1549
Size: 3316
Fragments arranged by size:
3789 3319 3316 2292 1305 1011 867 726
210
SbfI CC_TGCA'GG
Cuts at: 1766 4796 4979 6989 8267 13882 1766
Size: 3030 183 2010 1278 5615 4719
Fragments arranged by size:
5615 4719 3030 2010 1278 183
ScaI AGT'ACT
Cuts at: 9970 16352 9970
Size: 6382 10453
SexAI A'CCwGG_T
Cuts at: 13091 13091
Size: 16835
SfcI C'TryA_G
Cuts at: 33 487 1018 1390 1444 1469 1711 1762 1984
Size: 454 531 372 54 25 242 51 222
Cuts at: 1984 2287 2311 2410 2479 3069 3094 3169 3676
Size: 303 24 99 69 590 25 75 507
Cuts at: 3676 3856 3931 4155 4366 4453 4564 4612 4792
Size: 180 75 224 211 87 111 48 180
Cuts at: 4792 4816 4921 4975 5125 5404 5420 6295 6328
Size: 24 105 54 150 279 16 875 33
Cuts at: 6328 6382 6394 6590 6766 6814 6985 7183 7225
Size: 54 12 196 176 48 171 198 42
Cuts at: 7225 7594 7642 7660 7696 7906 7981 8161 8194
Size: 369 48 18 36 210 75 180 33
Cuts at: 8194 8263 8335 8782 9187 9729 9871 10240 10494
Size: 69 72 447 405 542 142 369 254
Cuts at: 10494 11914 12493 13423 13611 13620 13878 14563 15556
Size: 1420 579 930 188 9 258 685 993
Cuts at: 15556 15747 33
Size: 191 1121
Fragments arranged by size:
1420 1121 993 930 875 685 590 579
542 531 507 454 447 405 372 369
369 303 279 258 254 242 224 222
211 210 198 196 191 188 180 180
180 176 171 150 142 111 105 99
87 75 75 75 72 69 69 54
54 54 51 48 48 48 42 36
33 33 25 25 24 24 18 16
12 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2548 2788 3544 3574 4084 4795 5593 6310 6841
Size: 240 756 30 510 711 798 717 531
Cuts at: 6841 9449 13249 13303 2548
Size: 2608 3800 54 6080
Fragments arranged by size:
6080 3800 2608 798 756 717 711 531
510 240 54 30
SfoI GGC'GCC
Cuts at: 1729 3849 4840 6136 6256 12790 13476 14267 14515
Size: 2120 991 1296 120 6534 686 791 248
Cuts at: 14515 1729
Size: 4049
Fragments arranged by size:
6534 4049 2120 1296 991 791 686 248
120
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 16835
SgrAI Cr'CCGG_yG
Cuts at: 11605 12907 13165 11605
Size: 1302 258 15275
Fragments arranged by size:
15275 1302 258
SmaI CCC'GGG
Cuts at: 457 1948 6478 6571 8893 9568 13681 13857 457
Size: 1491 4530 93 2322 675 4113 176 3435
Fragments arranged by size:
4530 4113 3435 2322 1491 675 176 93
SmlI C'TyrA_G
Cuts at: 1252 1564 3451 3523 3562 3742 3793 4066 5095
Size: 312 1887 72 39 180 51 273 1029
Cuts at: 5095 5731 5755 6916 7123 7162 7342 7600 10180
Size: 636 24 1161 207 39 180 258 2580
Cuts at: 10180 11167 11218 11317 13513 13990 14905 15397 15659
Size: 987 51 99 2196 477 915 492 262
Cuts at: 15659 1252
Size: 2428
Fragments arranged by size:
2580 2428 2196 1887 1161 1029 987 915
636 492 477 312 273 262 258 207
180 180 99 72 51 51 39 39
24
SpeI A'CTAG_T
Cuts at: 67 67
Size: 16835
SphI G_CATG'C
Cuts at: 4380 13888 14388 4380
Size: 9508 500 6827
Fragments arranged by size:
9508 6827 500
SrfI GCCC'GGGC
Cuts at: 6571 6571
Size: 16835
StuI AGG'CCT
Cuts at: 853 1868 2537 2992 3181 3577 5809 9727 12658
Size: 1015 669 455 189 396 2232 3918 2931
Cuts at: 12658 13135 13252 853
Size: 477 117 4436
Fragments arranged by size:
4436 3918 2931 2232 1015 669 477 455
396 189 117
StyI C'CwwG_G
Cuts at: 204 588 668 1181 1205 2012 2552 3572 3842
Size: 384 80 513 24 807 540 1020 270
Cuts at: 3842 4097 4493 5462 6236 7106 7763 8738 8855
Size: 255 396 969 774 870 657 975 117
Cuts at: 8855 9709 9722 10283 10715 11030 11867 12017 12300
Size: 854 13 561 432 315 837 150 283
Cuts at: 12300 12449 13601 13969 14323 204
Size: 149 1152 368 354 2716
Fragments arranged by size:
2716 1152 1020 975 969 870 854 837
807 774 657 561 540 513 432 396
384 368 354 315 283 270 255 150
149 117 80 24 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1770 2597 3787 6796 7474 9907 12445 14418 14551
Size: 827 1190 3009 678 2433 2538 1973 133
Cuts at: 14551 15010 15219 1770
Size: 459 209 3386
Fragments arranged by size:
3386 3009 2538 2433 1973 1190 827 678
459 209 133
TatI w'GTAC_w
Cuts at: 1649 2516 4010 4184 9968 10859 16350 1649
Size: 867 1494 174 5784 891 5491 2134
Fragments arranged by size:
5784 5491 2134 1494 891 867 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1279 1311 2062 2094 7478 7510 10918 10950 11193
Size: 32 751 32 5384 32 3408 32 243
Cuts at: 11193 11225 12703 12735 16352 16384 1279
Size: 32 1478 32 3617 32 1730
Fragments arranged by size:
5384 3617 3408 1730 1478 751 243 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 13192 14632 13192
Size: 1440 15395
XbaI T'CTAG_A
Cuts at: 13866 13866
Size: 16835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1392 5550 7548 10137 10377 11874 12984 14330 1392
Size: 4158 1998 2589 240 1497 1110 1346 3897
Fragments arranged by size:
4158 3897 2589 1998 1497 1346 1110 240
XhoI C'TCGA_G
Cuts at: 3562 3793 11167 11218 3562
Size: 231 7374 51 9179
Fragments arranged by size:
9179 7374 231 51
XmaI C'CCGG_G
Cuts at: 455 1946 6476 6569 8891 9566 13679 13855 455
Size: 1491 4530 93 2322 675 4113 176 3435
Fragments arranged by size:
4530 4113 3435 2322 1491 675 176 93
XmnI GAAnn'nnTTC
Cuts at: 328 13252 328
Size: 12924 3911
ZraI GAC'GTC
Cuts at: 8898 12578 8898
Size: 3680 13155
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BfrBI BmtI BstZ17I FspAI
HpaI MfeI NdeI NheI NruI NsiI PacI PsiI
SnaBI SspI SwaI