Restriction map for
pF1KE2480
(Circular) (Six-base) MAPSORT of: pF1KE2480.seq Check: 8794 from: 1 to: 7472
pF1KE2480 7472 bp
With 182 enzymes: SgfI *
August 27, 2013 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3739 3739
Size: 7472
AccI GT'mk_AC
Cuts at: 1000 3798 4510 1000
Size: 2798 712 3962
Fragments arranged by size:
3962 2798 712
Acc65I G'GTAC_C
Cuts at: 4488 4488
Size: 7472
AclI AA'CG_TT
Cuts at: 7312 7312
Size: 7472
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 688 851 1225 1802 2503 2686 2767 3230
Size: 452 163 374 577 701 183 81 463
Cuts at: 3230 3505 3752 5297 5729 6476 6744 236
Size: 275 247 1545 432 747 268 964
Fragments arranged by size:
1545 964 747 701 577 463 452 432
374 275 268 247 183 163 81
AfeI AGC'GCT
Cuts at: 531 531
Size: 7472
AflIII A'CryG_T
Cuts at: 2014 2044 2271 3221 3223 4824 5928 6905 2014
Size: 30 227 950 2 1601 1104 977 2581
Fragments arranged by size:
2581 1601 1104 977 950 227 30 2
AgeI A'CCGG_T
Cuts at: 995 3046 5860 995
Size: 2051 2814 2607
Fragments arranged by size:
2814 2607 2051
AhdI GACnn_n'nnGTC
Cuts at: 1652 1652
Size: 7472
AlwNI CAG_nnn'CTG
Cuts at: 1549 1800 2926 4434 4777 6344 1549
Size: 251 1126 1508 343 1567 2677
Fragments arranged by size:
2677 1567 1508 1126 343 251
ApaLI G'TGCA_C
Cuts at: 6242 6242
Size: 7472
ApoI r'AATT_y
Cuts at: 431 746 978 1093 1198 1745 2808 3161 3620
Size: 315 232 115 105 547 1063 353 459
Cuts at: 3620 3970 4096 4476 6825 7081 431
Size: 350 126 380 2349 256 822
Fragments arranged by size:
2349 1063 822 547 459 380 353 350
315 256 232 126 115 105
AseI AT'TA_AT
Cuts at: 20 1031 2305 20
Size: 1011 1274 5187
Fragments arranged by size:
5187 1274 1011
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7472
AvaI C'yCGr_G
Cuts at: 3449 4492 3449
Size: 1043 6429
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2405 2438 2405
Size: 33 7439
BamHI G'GATC_C
Cuts at: 4497 4497
Size: 7472
BanI G'GyrC_C
Cuts at: 992 4488 4902 5150 5185 992
Size: 3496 414 248 35 3279
Fragments arranged by size:
3496 3279 414 248 35
BanII G_rGCy'C
Cuts at: 3450 4486 5516 3450
Size: 1036 1030 5406
Fragments arranged by size:
5406 1036 1030
BbeI G_GCGC'C
Cuts at: 4906 5154 4906
Size: 248 7224
BbsI GAAGACnn'nnnn_
Cuts at: 979 2449 3866 979
Size: 1470 1417 4585
Fragments arranged by size:
4585 1470 1417
BciVI GTATCCnnnnn_n'
Cuts at: 2406 3647 5370 6131 7472 2406
Size: 1241 1723 761 1341 2406
Fragments arranged by size:
2406 1723 1341 1241 761
BclI T'GATC_A
Cuts at: 110 4992 110
Size: 4882 2590
BfrBI ATG'CAT
Cuts at: 590 1420 590
Size: 830 6642
BglII A'GATC_T
Cuts at: 3957 4066 4987 7024 3957
Size: 109 921 2037 4405
Fragments arranged by size:
4405 2037 921 109
BlpI GC'TnA_GC
Cuts at: 2341 3609 4584 2341
Size: 1268 975 5229
Fragments arranged by size:
5229 1268 975
Bme1580I G_kGCm'C
Cuts at: 2022 5097 5190 6246 2022
Size: 3075 93 1056 3248
Fragments arranged by size:
3248 3075 1056 93
BmgBI CAC'GTC
Cuts at: 2047 2047
Size: 7472
BmrI ACTGGGnnnn_n'
Cuts at: 1113 3104 4684 4765 5100 7244 1113
Size: 1991 1580 81 335 2144 1341
Fragments arranged by size:
2144 1991 1580 1341 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5839 5839
Size: 7472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 1571 2048 3667 3826 4108 4135 625
Size: 946 477 1619 159 282 27 3962
Fragments arranged by size:
3962 1619 946 477 282 159 27
Bpu10I CC'TnA_GC
Cuts at: 4222 4222
Size: 7472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2367 2725 3400 4648 5527 6019 6317 2367
Size: 358 675 1248 879 492 298 3522
Fragments arranged by size:
3522 1248 879 675 492 358 298
BsaI GGTCTCn'nnnn_
Cuts at: 3395 4290 7176 3395
Size: 895 2886 3691
Fragments arranged by size:
3691 2886 895
BsaAI yAC'GTr
Cuts at: 2017 3224 4825 2017
Size: 1207 1601 4664
Fragments arranged by size:
4664 1601 1207
BsaBI GATnn'nnATC
Cuts at: 6 4986 7023 6
Size: 4980 2037 455
Fragments arranged by size:
4980 2037 455
BsaHI Gr'CG_yC
Cuts at: 4395 4903 5151 5853 7349 4395
Size: 508 248 702 1496 4518
Fragments arranged by size:
4518 1496 702 508 248
BsaWI w'CCGG_w
Cuts at: 995 3046 3129 4132 4661 5182 5860 6134 6281
Size: 2051 83 1003 529 521 678 274 147
Cuts at: 6281 6692 995
Size: 411 1775
Fragments arranged by size:
2051 1775 1003 678 529 521 411 274
147 83
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 401 1281 1678 401
Size: 880 397 6195
Fragments arranged by size:
6195 880 397
BseYI C'CCAG_C
Cuts at: 532 4897 5655 6232 6728 532
Size: 4365 758 577 496 1276
Fragments arranged by size:
4365 1276 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 2862 1057
Size: 1805 5667
BsiEI CG_ry'CG
Cuts at: 104 5014 5060 6268 104
Size: 4910 46 1208 1308
Fragments arranged by size:
4910 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1839 3450 3724 4290 4486 5264 5454 6246 1839
Size: 1611 274 566 196 778 190 792 3065
Fragments arranged by size:
3065 1611 792 778 566 274 196 190
BsmI GAATG_Cn'
Cuts at: 1124 1124
Size: 7472
BsmBI CGTCTCn'nnnn_
Cuts at: 4402 4402
Size: 7472
Bsp1286I G_dGCh'C
Cuts at: 1839 2022 3450 3724 4290 4486 5097 5190 5264
Size: 183 1428 274 566 196 611 93 74
Cuts at: 5264 5454 5516 6246 1839
Size: 190 62 730 3065
Fragments arranged by size:
3065 1428 730 611 566 274 196 190
183 93 74 62
BspEI T'CCGG_A
Cuts at: 6692 6692
Size: 7472
BspHI T'CATG_A
Cuts at: 6648 6648
Size: 7472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3666 3739 3767 4522 4736 5038 5419 3666
Size: 73 28 755 214 302 381 5719
Fragments arranged by size:
5719 755 381 302 214 73 28
BsrBI CCG'CTC
Cuts at: 50 5764 5818 7302 7467 50
Size: 5714 54 1484 165 55
Fragments arranged by size:
5714 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3308 4150 5384 3308
Size: 842 1234 5396
Fragments arranged by size:
5396 1234 842
BsrFI r'CCGG_y
Cuts at: 995 3046 5470 5651 5860 995
Size: 2051 2424 181 209 2607
Fragments arranged by size:
2607 2424 2051 209 181
BsrGI T'GTAC_A
Cuts at: 1427 1427
Size: 7472
BssSI C'ACGA_G
Cuts at: 3763 5743 6101 3763
Size: 1980 358 5134
Fragments arranged by size:
5134 1980 358
BstAPI GCAn_nnn'nTGC
Cuts at: 406 2840 4434 4446 4849 406
Size: 2434 1594 12 403 3029
Fragments arranged by size:
3029 2434 1594 403 12
BstBI TT'CG_AA
Cuts at: 5833 5833
Size: 7472
BstEII G'GTnAC_C
Cuts at: 3148 3148
Size: 7472
BstXI CCAn_nnnn'nTGG
Cuts at: 426 426
Size: 7472
BstYI r'GATC_y
Cuts at: 1 1677 2362 3957 4066 4497 4964 4987 5322
Size: 1676 685 1595 109 431 467 23 335
Cuts at: 5322 5568 6580 6666 6678 7024 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1676 1595 1012 685 467 449 431 346
335 246 109 86 23 12
BstZ17I GTA'TAC
Cuts at: 3799 3799
Size: 7472
BtgI C'CryG_G
Cuts at: 695 4194 695
Size: 3499 3973
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5422 5603 5766 5422
Size: 181 163 7128
Fragments arranged by size:
7128 181 163
BtsI GCAGTG_nn'
Cuts at: 1045 2850 3597 4715 1045
Size: 1805 747 1118 3802
Fragments arranged by size:
3802 1805 1118 747
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 209 244 209
Size: 35 7437
DraI TTT'AAA
Cuts at: 792 1151 1770 1989 4471 792
Size: 359 619 219 2482 3793
Fragments arranged by size:
3793 2482 619 359 219
DrdI GACnn_nn'nnGTC
Cuts at: 5178 6036 5178
Size: 858 6614
EaeI y'GGCC_r
Cuts at: 158 416 3594 5057 5231 5622 5649 6994 158
Size: 258 3178 1463 174 391 27 1345 636
Fragments arranged by size:
3178 1463 1345 636 391 258 174 27
EagI C'GGCC_G
Cuts at: 5057 5057
Size: 7472
EarI CTCTTCn'nnn_
Cuts at: 1244 3206 3728 5495 5705 1244
Size: 1962 522 1767 210 3011
Fragments arranged by size:
3011 1962 1767 522 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5680 5990 6136 7282 5680
Size: 310 146 1146 5870
Fragments arranged by size:
5870 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3448 4484 3448
Size: 1036 6436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 625 688 851 1225 1571 1802 2048 2503
Size: 389 63 163 374 346 231 246 455
Cuts at: 2503 2686 2767 3230 3505 3667 3752 3826 4108
Size: 183 81 463 275 162 85 74 282
Cuts at: 4108 4135 5297 5729 6476 6744 236
Size: 27 1162 432 747 268 964
Fragments arranged by size:
1162 964 747 463 455 432 389 374
346 282 275 268 246 231 183 163
162 85 81 74 63 27
EcoO109I rG'GnC_Cy
Cuts at: 4611 4611
Size: 7472
EcoRI G'AATT_C
Cuts at: 978 1745 4476 978
Size: 767 2731 3974
Fragments arranged by size:
3974 2731 767
EcoRV GAT'ATC
Cuts at: 3002 3002
Size: 7472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2361 2514 5491 2361
Size: 153 2977 4342
Fragments arranged by size:
4342 2977 153
FspI TGC'GCA
Cuts at: 5253 5253
Size: 7472
HaeII r_GCGC'y
Cuts at: 533 4906 5154 6176 7155 533
Size: 4373 248 1022 979 850
Fragments arranged by size:
4373 1022 979 850 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1638 1670 2637 2669 2698 2730 2772 2804 3081
Size: 32 967 32 29 32 42 32 277
Cuts at: 3081 3113 3373 3405 5314 5344 5346 5376 1638
Size: 32 260 32 1909 30 2 30 3734
Fragments arranged by size:
3734 1909 967 277 260 42 32 32
32 32 32 32 30 30 29 2
HincII GTy'rAC
Cuts at: 2563 2852 3282 3406 4511 2563
Size: 289 430 124 1105 5524
Fragments arranged by size:
5524 1105 430 289 124
HindIII A'AGCT_T
Cuts at: 500 2343 500
Size: 1843 5629
HpaI GTT'AAC
Cuts at: 2852 2852
Size: 7472
KasI G'GCGC_C
Cuts at: 4902 5150 4902
Size: 248 7224
KpnI G_GTAC'C
Cuts at: 4492 4492
Size: 7472
MfeI C'AATT_G
Cuts at: 1532 1872 2110 1532
Size: 340 238 6894
Fragments arranged by size:
6894 340 238
MluI A'CGCG_T
Cuts at: 6905 6905
Size: 7472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3838 6143 6327 3838
Size: 2305 184 4983
Fragments arranged by size:
4983 2305 184
MscI TGG'CCA
Cuts at: 160 418 3596 5233 160
Size: 258 3178 1637 2399
Fragments arranged by size:
3178 2399 1637 258
MslI CAynn'nnrTG
Cuts at: 2530 3251 4281 5461 5588 2530
Size: 721 1030 1180 127 4414
Fragments arranged by size:
4414 1180 1030 721 127
MspA1I CmG'CkG
Cuts at: 3243 4272 4583 4897 5257 6270 6515 3243
Size: 1029 311 314 360 1013 245 4200
Fragments arranged by size:
4200 1029 1013 360 314 311 245
NaeI GCC'GGC
Cuts at: 5653 5653
Size: 7472
NarI GG'CG_CC
Cuts at: 4903 5151 4903
Size: 248 7224
NcoI C'CATG_G
Cuts at: 695 4194 695
Size: 3499 3973
NgoMIV G'CCGG_C
Cuts at: 5651 5651
Size: 7472
NsiI A_TGCA'T
Cuts at: 592 1422 592
Size: 830 6642
NspI r_CATG'y
Cuts at: 1289 4525 5025 5932 1289
Size: 3236 500 907 2829
Fragments arranged by size:
3236 2829 907 500
PasI CC'CwG_GG
Cuts at: 3261 3261
Size: 7472
PciI A'CATG_T
Cuts at: 5928 5928
Size: 7472
PflMI CCAn_nnn'nTGG
Cuts at: 955 955
Size: 7472
PfoI T'CCnGG_A
Cuts at: 1277 1580 1277
Size: 303 7169
PmeI GTTT'AAAC
Cuts at: 4471 4471
Size: 7472
PmlI CAC'GTG
Cuts at: 2017 3224 2017
Size: 1207 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 182 214 6611 6643 182
Size: 32 6397 32 1011
Fragments arranged by size:
6397 1011 32 32
PsiI TTA'TAA
Cuts at: 1933 1933
Size: 7472
PspXI vC'TCGA_Gb
Cuts at: 3449 3449
Size: 7472
PstI C_TGCA'G
Cuts at: 1359 4439 4519 5204 1359
Size: 3080 80 685 3627
Fragments arranged by size:
3627 3080 685 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7472
PvuII CAG'CTG
Cuts at: 4272 4897 5257 4272
Size: 625 360 6487
Fragments arranged by size:
6487 625 360
RsrII CG'GwC_CG
Cuts at: 5667 5667
Size: 7472
SacI G_AGCT'C
Cuts at: 3450 4486 3450
Size: 1036 6436
SalI G'TCGA_C
Cuts at: 4509 4509
Size: 7472
SapI GCTCTTCn'nnn_
Cuts at: 5495 5705 5495
Size: 210 7262
SbfI CC_TGCA'GG
Cuts at: 4519 4519
Size: 7472
ScaI AGT'ACT
Cuts at: 1701 6989 1701
Size: 5288 2184
SexAI A'CCwGG_T
Cuts at: 2004 2004
Size: 7472
SfcI C'TryA_G
Cuts at: 33 1355 2168 4435 4515 5200 6193 6384 33
Size: 1322 813 2267 80 685 993 191 1121
Fragments arranged by size:
2267 1322 1121 993 813 685 191 80
SfoI GGC'GCC
Cuts at: 4904 5152 4904
Size: 248 7224
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7472
SmaI CCC'GGG
Cuts at: 4494 4494
Size: 7472
SmlI C'TyrA_G
Cuts at: 2346 2704 3379 3449 4627 5542 6034 6296 2346
Size: 358 675 70 1178 915 492 262 3522
Fragments arranged by size:
3522 1178 915 675 492 358 262 70
SpeI A'CTAG_T
Cuts at: 67 879 3988 67
Size: 812 3109 3551
Fragments arranged by size:
3551 3109 812
SphI G_CATG'C
Cuts at: 4525 5025 4525
Size: 500 6972
SspI AAT'ATT
Cuts at: 405 1296 405
Size: 891 6581
StuI AGG'CCT
Cuts at: 394 4390 394
Size: 3996 3476
StyI C'CwwG_G
Cuts at: 170 367 395 695 2606 4143 4194 4606 4960
Size: 197 28 300 1911 1537 51 412 354
Cuts at: 4960 170
Size: 2682
Fragments arranged by size:
2682 1911 1537 412 354 300 197 51
28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 5055 5188 5647 5856 5055
Size: 133 459 209 6671
Fragments arranged by size:
6671 459 209 133
TatI w'GTAC_w
Cuts at: 132 1427 1699 1827 1944 3340 3375 3395 6987
Size: 1295 272 128 117 1396 35 20 3592
Cuts at: 6987 132
Size: 617
Fragments arranged by size:
3592 1396 1295 617 272 128 117 35
20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 279 311 4148 4180 6989 7021 279
Size: 32 3837 32 2809 32 730
Fragments arranged by size:
3837 2809 730 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2006 5269 2006
Size: 3263 4209
XbaI T'CTAG_A
Cuts at: 1680 4503 1680
Size: 2823 4649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 168 2029 4086 4192 4967 168
Size: 1861 2057 106 775 2673
Fragments arranged by size:
2673 2057 1861 775 106
XhoI C'TCGA_G
Cuts at: 3449 3449
Size: 7472
XmaI C'CCGG_G
Cuts at: 4492 4492
Size: 7472
XmnI GAAnn'nnTTC
Cuts at: 3518 3518
Size: 7472
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PsiI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AlfI AloI ApaI AscI AvrII
BbvCI BcgI BglI BmtI BsaXI BsiWI BssHII Bsu36I
ClaI DraIII EcoNI FseI FspAI NdeI NheI NotI
NruI PacI PpuMI PshAI PspOMI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SwaI ZraI