Restriction map for
pF1KE2455
(Circular) (Six-base) MAPSORT of: pF1KE2455.seq Check: 5353 from: 1 to: 4550
pF1KE2455 4550 bp
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 420 420
Size: 4550
AccI GT'mk_AC
Cuts at: 1588 1588
Size: 4550
Acc65I G'GTAC_C
Cuts at: 374 1311 1566 374
Size: 937 255 3358
Fragments arranged by size:
3358 937 255
AclI AA'CG_TT
Cuts at: 4390 4390
Size: 4550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2375 2807 3554 3822 2375
Size: 432 747 268 3103
Fragments arranged by size:
3103 747 432 268
AflIII A'CryG_T
Cuts at: 1902 3006 3983 1902
Size: 1104 977 2469
Fragments arranged by size:
2469 1104 977
AgeI A'CCGG_T
Cuts at: 2938 2938
Size: 4550
AleI CACnn'nnGTG
Cuts at: 1378 1378
Size: 4550
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 274 274
Size: 4550
AlwNI CAG_nnn'CTG
Cuts at: 1855 3422 1855
Size: 1567 2983
ApaI G_GGCC'C
Cuts at: 154 1235 154
Size: 1081 3469
ApaLI G'TGCA_C
Cuts at: 3320 3320
Size: 4550
ApoI r'AATT_y
Cuts at: 589 1554 3903 4159 589
Size: 965 2349 256 980
Fragments arranged by size:
2349 980 965 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4550
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
AvaI C'yCGr_G
Cuts at: 153 483 890 979 1570 153
Size: 330 407 89 591 3133
Fragments arranged by size:
3133 591 407 330 89
BamHI G'GATC_C
Cuts at: 1575 1575
Size: 4550
BanI G'GyrC_C
Cuts at: 374 403 1311 1566 1980 2228 2263 374
Size: 29 908 255 414 248 35 2661
Fragments arranged by size:
2661 908 414 255 248 35 29
BanII G_rGCy'C
Cuts at: 154 1235 1564 2594 154
Size: 1081 329 1030 2110
Fragments arranged by size:
2110 1081 1030 329
BbeI G_GCGC'C
Cuts at: 1984 2232 1984
Size: 248 4302
BbvCI CC'TCA_GC
Cuts at: 696 696
Size: 4550
BciVI GTATCCnnnnn_n'
Cuts at: 2448 3209 4550 2448
Size: 761 1341 2448
Fragments arranged by size:
2448 1341 761
BclI T'GATC_A
Cuts at: 2070 2070
Size: 4550
BglI GCCn_nnn'nGGC
Cuts at: 871 871
Size: 4550
BglII A'GATC_T
Cuts at: 2065 4102 2065
Size: 2037 2513
BlpI GC'TnA_GC
Cuts at: 1662 1662
Size: 4550
Bme1580I G_kGCm'C
Cuts at: 134 154 1235 2175 2268 3324 134
Size: 20 1081 940 93 1056 1360
Fragments arranged by size:
1360 1081 1056 940 93 20
BmgBI CAC'GTC
Cuts at: 447 447
Size: 4550
BmrI ACTGGGnnnn_n'
Cuts at: 1762 1843 2178 4322 1762
Size: 81 335 2144 1990
Fragments arranged by size:
2144 1990 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 405 711 2917 405
Size: 306 2206 2038
Fragments arranged by size:
2206 2038 306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 829 820
Size: 9 4541
Bpu10I CC'TnA_GC
Cuts at: 682 696 682
Size: 14 4536
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 1726 2605 3097 3395 505
Size: 1221 879 492 298 1660
Fragments arranged by size:
1660 1221 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1027 4254 1027
Size: 3227 1323
BsaAI yAC'GTr
Cuts at: 1903 1903
Size: 4550
BsaBI GATnn'nnATC
Cuts at: 6 2064 4101 6
Size: 2058 2037 455
Fragments arranged by size:
2058 2037 455
BsaHI Gr'CG_yC
Cuts at: 1981 2229 2931 4427 1981
Size: 248 702 1496 2104
Fragments arranged by size:
2104 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1739 2260 2938 3212 3359 3770 1739
Size: 521 678 274 147 411 2519
Fragments arranged by size:
2519 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1327 1357 1327
Size: 30 4520
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 622 625 622
Size: 3 4547
BseYI C'CCAG_C
Cuts at: 497 940 1078 1177 1975 2733 3310 3806 497
Size: 443 138 99 798 758 577 496 1241
Fragments arranged by size:
1241 798 758 577 496 443 138 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 390
Size: 4550
BsiEI CG_ry'CG
Cuts at: 104 148 2092 2138 3346 104
Size: 44 1944 46 1208 1308
Fragments arranged by size:
1944 1308 1208 46 44
BsiHKAI G_wGCw'C
Cuts at: 1564 2342 2532 3324 1564
Size: 778 190 792 2790
Fragments arranged by size:
2790 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 360 360
Size: 4550
Bsp1286I G_dGCh'C
Cuts at: 134 154 1235 1564 2175 2268 2342 2532 2594
Size: 20 1081 329 611 93 74 190 62
Cuts at: 2594 3324 134
Size: 730 1360
Fragments arranged by size:
1360 1081 730 611 329 190 93 74
62 20
BspEI T'CCGG_A
Cuts at: 3770 3770
Size: 4550
BspHI T'CATG_A
Cuts at: 3726 3726
Size: 4550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 362 420 1600 1814 2116 2497 362
Size: 58 1180 214 302 381 2415
Fragments arranged by size:
2415 1180 381 302 214 58
BsrBI CCG'CTC
Cuts at: 50 2842 2896 4380 4545 50
Size: 2792 54 1484 165 55
Fragments arranged by size:
2792 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1269 2462 1269
Size: 1193 3357
BsrFI r'CCGG_y
Cuts at: 121 2548 2729 2938 121
Size: 2427 181 209 1733
Fragments arranged by size:
2427 1733 209 181
BsrGI T'GTAC_A
Cuts at: 1122 1122
Size: 4550
BssSI C'ACGA_G
Cuts at: 2821 3179 2821
Size: 358 4192
BstAPI GCAn_nnn'nTGC
Cuts at: 1927 1927
Size: 4550
BstBI TT'CG_AA
Cuts at: 2911 2911
Size: 4550
BstXI CCAn_nnnn'nTGG
Cuts at: 385 385
Size: 4550
BstYI r'GATC_y
Cuts at: 1 362 1074 1575 2042 2065 2400 2646 3658
Size: 361 712 501 467 23 335 246 1012
Cuts at: 3658 3744 3756 4102 1
Size: 86 12 346 449
Fragments arranged by size:
1012 712 501 467 449 361 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1277 1277
Size: 4550
BtgI C'CryG_G
Cuts at: 300 345 1302 1376 300
Size: 45 957 74 3474
Fragments arranged by size:
3474 957 74 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2500 2681 2844 2500
Size: 181 163 4206
Fragments arranged by size:
4206 181 163
BtsI GCAGTG_nn'
Cuts at: 995 1793 995
Size: 798 3752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1287 1322 1287
Size: 35 4515
DraI TTT'AAA
Cuts at: 1549 1549
Size: 4550
DrdI GACnn_nn'nnGTC
Cuts at: 2256 3114 2256
Size: 858 3692
EaeI y'GGCC_r
Cuts at: 119 145 207 1385 2135 2309 2700 2727 4072
Size: 26 62 1178 750 174 391 27 1345
Cuts at: 4072 119
Size: 597
Fragments arranged by size:
1345 1178 750 597 391 174 62 27
26
EagI C'GGCC_G
Cuts at: 145 2135 145
Size: 1990 2560
EarI CTCTTCn'nnn_
Cuts at: 599 1411 2573 2783 599
Size: 812 1162 210 2366
Fragments arranged by size:
2366 1162 812 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 262 2758 3068 3214 4360 262
Size: 2496 310 146 1146 452
Fragments arranged by size:
2496 1146 452 310 146
EcoICRI GAG'CTC
Cuts at: 1562 1562
Size: 4550
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 829 2375 2807 3554 3822 820
Size: 9 1546 432 747 268 1548
Fragments arranged by size:
1548 1546 747 432 268 9
EcoNI CCTnn'n_nnAGG
Cuts at: 208 1496 208
Size: 1288 3262
EcoO109I rG'GnC_Cy
Cuts at: 1231 1232 1689 1231
Size: 1 457 4092
Fragments arranged by size:
4092 457 1
EcoRI G'AATT_C
Cuts at: 1554 1554
Size: 4550
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1550 2569 1550
Size: 1019 3531
FspI TGC'GCA
Cuts at: 2331 2331
Size: 4550
HaeII r_GCGC'y
Cuts at: 128 1984 2232 3254 4233 128
Size: 1856 248 1022 979 445
Fragments arranged by size:
1856 1022 979 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 703 715 735 747 884 916 1144 1176 2392
Size: 12 20 12 137 32 228 32 1216
Cuts at: 2392 2422 2424 2454 703
Size: 30 2 30 2799
Fragments arranged by size:
2799 1216 228 137 32 32 30 30
20 12 12 2
HincII GTy'rAC
Cuts at: 222 1589 222
Size: 1367 3183
KasI G'GCGC_C
Cuts at: 1980 2228 1980
Size: 248 4302
KpnI G_GTAC'C
Cuts at: 378 1315 1570 378
Size: 937 255 3358
Fragments arranged by size:
3358 937 255
MluI A'CGCG_T
Cuts at: 3983 3983
Size: 4550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1010 1169 1262 3221 3405 1010
Size: 159 93 1959 184 2155
Fragments arranged by size:
2155 1959 184 159 93
MscI TGG'CCA
Cuts at: 2311 2311
Size: 4550
MslI CAynn'nnrTG
Cuts at: 1000 1378 2539 2666 1000
Size: 378 1161 127 2884
Fragments arranged by size:
2884 1161 378 127
MspA1I CmG'CkG
Cuts at: 186 258 333 497 798 1026 1391 1661 1975
Size: 72 75 164 301 228 365 270 314
Cuts at: 1975 2335 3348 3593 186
Size: 360 1013 245 1143
Fragments arranged by size:
1143 1013 365 360 314 301 270 245
228 164 75 72
NaeI GCC'GGC
Cuts at: 123 2731 123
Size: 2608 1942
NarI GG'CG_CC
Cuts at: 1981 2229 1981
Size: 248 4302
NdeI CA'TA_TG
Cuts at: 1412 1412
Size: 4550
NgoMIV G'CCGG_C
Cuts at: 121 2729 121
Size: 2608 1942
NspI r_CATG'y
Cuts at: 492 1603 2103 3010 492
Size: 1111 500 907 2032
Fragments arranged by size:
2032 1111 907 500
PciI A'CATG_T
Cuts at: 3006 3006
Size: 4550
PmeI GTTT'AAAC
Cuts at: 1549 1549
Size: 4550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 3689
Size: 32 4518
PspOMI G'GGCC_C
Cuts at: 150 1231 150
Size: 1081 3469
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 728 760 728
Size: 32 4518
PstI C_TGCA'G
Cuts at: 1396 1597 2282 1396
Size: 201 685 3664
Fragments arranged by size:
3664 685 201
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4550
PvuII CAG'CTG
Cuts at: 333 497 798 1026 1391 1975 2335 333
Size: 164 301 228 365 584 360 2548
Fragments arranged by size:
2548 584 365 360 301 228 164
RsrII CG'GwC_CG
Cuts at: 2745 2745
Size: 4550
SacI G_AGCT'C
Cuts at: 1564 1564
Size: 4550
SalI G'TCGA_C
Cuts at: 1587 1587
Size: 4550
SapI GCTCTTCn'nnn_
Cuts at: 1411 2573 2783 1411
Size: 1162 210 3178
Fragments arranged by size:
3178 1162 210
SbfI CC_TGCA'GG
Cuts at: 1597 1597
Size: 4550
ScaI AGT'ACT
Cuts at: 4067 4067
Size: 4550
SfcI C'TryA_G
Cuts at: 33 1392 1464 1479 1593 2278 3271 3462 33
Size: 1359 72 15 114 685 993 191 1121
Fragments arranged by size:
1359 1121 993 685 191 114 72 15
SfoI GGC'GCC
Cuts at: 1982 2230 1982
Size: 248 4302
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
SmaI CCC'GGG
Cuts at: 155 485 981 1572 155
Size: 330 496 591 3133
Fragments arranged by size:
3133 591 496 330
SmlI C'TyrA_G
Cuts at: 520 1705 2620 3112 3374 520
Size: 1185 915 492 262 1696
Fragments arranged by size:
1696 1185 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4550
SphI G_CATG'C
Cuts at: 1603 2103 1603
Size: 500 4050
StyI C'CwwG_G
Cuts at: 1534 1684 2038 1534
Size: 150 354 4046
Fragments arranged by size:
4046 354 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1473 2133 2266 2725 2934 1473
Size: 660 133 459 209 3089
Fragments arranged by size:
3089 660 459 209 133
TatI w'GTAC_w
Cuts at: 894 1122 4065 894
Size: 228 2943 1379
Fragments arranged by size:
2943 1379 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1041 1073 4067 4099 1041
Size: 32 2994 32 1492
Fragments arranged by size:
2994 1492 32 32
Tth111I GACn'n_nGTC
Cuts at: 529 1492 2347 529
Size: 963 855 2732
Fragments arranged by size:
2732 963 855
XbaI T'CTAG_A
Cuts at: 1581 1581
Size: 4550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 954 2045 114
Size: 840 1091 2619
Fragments arranged by size:
2619 1091 840
XmaI C'CCGG_G
Cuts at: 153 483 979 1570 153
Size: 330 496 591 3133
Fragments arranged by size:
3133 591 496 330
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PmeI PpiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsiWI BsmI BssHII BstEII
BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI