Restriction map for
pF1KE2445
(Circular) (Six-base) MAPSORT of: pF1KE2445.seq Check: 4020 from: 1 to: 4460
pF1KE2445 4460 bp
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AccI GT'mk_AC
Cuts at: 1498 1498
Size: 4460
Acc65I G'GTAC_C
Cuts at: 1476 1476
Size: 4460
AclI AA'CG_TT
Cuts at: 4300 4300
Size: 4460
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 777 1309 2285 2717 3464 3732 777
Size: 532 976 432 747 268 1505
Fragments arranged by size:
1505 976 747 532 432 268
AflII C'TTAA_G
Cuts at: 205 205
Size: 4460
AflIII A'CryG_T
Cuts at: 1273 1812 2916 3893 1273
Size: 539 1104 977 1840
Fragments arranged by size:
1840 1104 977 539
AgeI A'CCGG_T
Cuts at: 2848 2848
Size: 4460
AhdI GACnn_n'nnGTC
Cuts at: 245 245
Size: 4460
AleI CACnn'nnGTG
Cuts at: 168 168
Size: 4460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 186 186
Size: 4460
AlwNI CAG_nnn'CTG
Cuts at: 309 1765 3332 309
Size: 1456 1567 1437
Fragments arranged by size:
1567 1456 1437
ApaLI G'TGCA_C
Cuts at: 3230 3230
Size: 4460
ApoI r'AATT_y
Cuts at: 211 753 872 975 1078 1464 3813 4069 211
Size: 542 119 103 103 386 2349 256 602
Fragments arranged by size:
2349 602 542 386 256 119 103 103
AseI AT'TA_AT
Cuts at: 20 20
Size: 4460
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
AvaI C'yCGr_G
Cuts at: 1480 1480
Size: 4460
BamHI G'GATC_C
Cuts at: 1485 1485
Size: 4460
BanI G'GyrC_C
Cuts at: 1187 1476 1890 2138 2173 1187
Size: 289 414 248 35 3474
Fragments arranged by size:
3474 414 289 248 35
BanII G_rGCy'C
Cuts at: 1474 2504 1474
Size: 1030 3430
BbeI G_GCGC'C
Cuts at: 1894 2142 1894
Size: 248 4212
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4426
BciVI GTATCCnnnnn_n'
Cuts at: 2358 3119 4460 2358
Size: 761 1341 2358
Fragments arranged by size:
2358 1341 761
BclI T'GATC_A
Cuts at: 1980 1980
Size: 4460
BglII A'GATC_T
Cuts at: 139 675 1347 1975 4012 139
Size: 536 672 628 2037 587
Fragments arranged by size:
2037 672 628 587 536
BlpI GC'TnA_GC
Cuts at: 1572 1572
Size: 4460
Bme1580I G_kGCm'C
Cuts at: 1383 2085 2178 3234 1383
Size: 702 93 1056 2609
Fragments arranged by size:
2609 1056 702 93
BmrI ACTGGGnnnn_n'
Cuts at: 912 1245 1672 1753 2088 4232 912
Size: 333 427 81 335 2144 1140
Fragments arranged by size:
2144 1140 427 335 333 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2827 2827
Size: 4460
Bpu10I CC'TnA_GC
Cuts at: 1285 1285
Size: 4460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 656 1221 1636 2515 3007 3305 656
Size: 565 415 879 492 298 1811
Fragments arranged by size:
1811 879 565 492 415 298
BsaI GGTCTCn'nnnn_
Cuts at: 537 4164 537
Size: 3627 833
BsaAI yAC'GTr
Cuts at: 1813 1813
Size: 4460
BsaBI GATnn'nnATC
Cuts at: 6 650 1405 1974 4011 6
Size: 644 755 569 2037 455
Fragments arranged by size:
2037 755 644 569 455
BsaHI Gr'CG_yC
Cuts at: 1891 2139 2841 4337 1891
Size: 248 702 1496 2014
Fragments arranged by size:
2014 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1649 2170 2848 3122 3269 3680 1649
Size: 521 678 274 147 411 2429
Fragments arranged by size:
2429 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 794 824 794
Size: 30 4430
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 112 112
Size: 4460
BseYI C'CCAG_C
Cuts at: 1120 1437 1885 2643 3220 3716 1120
Size: 317 448 758 577 496 1864
Fragments arranged by size:
1864 758 577 496 448 317
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 609
Size: 4460
BsiEI CG_ry'CG
Cuts at: 104 2002 2048 3256 104
Size: 1898 46 1208 1308
Fragments arranged by size:
1898 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1474 2252 2442 3234 1474
Size: 778 190 792 2700
Fragments arranged by size:
2700 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1383 1474 2085 2178 2252 2442 2504 3234 1383
Size: 91 611 93 74 190 62 730 2609
Fragments arranged by size:
2609 730 611 190 93 91 74 62
BspEI T'CCGG_A
Cuts at: 3680 3680
Size: 4460
BspHI T'CATG_A
Cuts at: 1143 1310 3636 1143
Size: 167 2326 1967
Fragments arranged by size:
2326 1967 167
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1510 1724 2026 2407 1510
Size: 214 302 381 3563
Fragments arranged by size:
3563 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2752 2806 4290 4455 50
Size: 2702 54 1484 165 55
Fragments arranged by size:
2702 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2372 2372
Size: 4460
BsrFI r'CCGG_y
Cuts at: 2458 2639 2848 2458
Size: 181 209 4070
Fragments arranged by size:
4070 209 181
BssSI C'ACGA_G
Cuts at: 2731 3089 2731
Size: 358 4102
BstAPI GCAn_nnn'nTGC
Cuts at: 1837 1837
Size: 4460
BstBI TT'CG_AA
Cuts at: 2821 2821
Size: 4460
BstYI r'GATC_y
Cuts at: 1 139 305 675 1347 1485 1952 1975 2310
Size: 138 166 370 672 138 467 23 335
Cuts at: 2310 2556 3568 3654 3666 4012 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 672 467 449 370 346 335 246
166 138 138 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1106 1106
Size: 4460
BtgI C'CryG_G
Cuts at: 131 687 131
Size: 556 3904
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2410 2591 2754 2410
Size: 181 163 4116
Fragments arranged by size:
4116 181 163
BtsI GCAGTG_nn'
Cuts at: 621 1329 1703 621
Size: 708 374 3378
Fragments arranged by size:
3378 708 374
DraI TTT'AAA
Cuts at: 1115 1459 1115
Size: 344 4116
DrdI GACnn_nn'nnGTC
Cuts at: 2166 3024 2166
Size: 858 3602
EaeI y'GGCC_r
Cuts at: 134 1293 2045 2219 2610 2637 3982 134
Size: 1159 752 174 391 27 1345 612
Fragments arranged by size:
1345 1159 752 612 391 174 27
EagI C'GGCC_G
Cuts at: 2045 2045
Size: 4460
EarI CTCTTCn'nnn_
Cuts at: 535 1293 1369 2483 2693 535
Size: 758 76 1114 210 2302
Fragments arranged by size:
2302 1114 758 210 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2668 2978 3124 4270 2668
Size: 310 146 1146 2858
Fragments arranged by size:
2858 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1472 1472
Size: 4460
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 777 1309 2285 2717 3464 3732 777
Size: 532 976 432 747 268 1505
Fragments arranged by size:
1505 976 747 532 432 268
EcoO109I rG'GnC_Cy
Cuts at: 612 1599 612
Size: 987 3473
EcoRI G'AATT_C
Cuts at: 1464 1464
Size: 4460
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4460
FspI TGC'GCA
Cuts at: 2241 2241
Size: 4460
HaeII r_GCGC'y
Cuts at: 1894 2142 3164 4143 1894
Size: 248 1022 979 2211
Fragments arranged by size:
2211 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 231 263 667 699 928 960 1292 1324 2302
Size: 32 404 32 229 32 332 32 978
Cuts at: 2302 2332 2334 2364 231
Size: 30 2 30 2327
Fragments arranged by size:
2327 978 404 332 229 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1499 1499
Size: 4460
HindIII A'AGCT_T
Cuts at: 252 708 252
Size: 456 4004
KasI G'GCGC_C
Cuts at: 1890 2138 1890
Size: 248 4212
KpnI G_GTAC'C
Cuts at: 1480 1480
Size: 4460
MluI A'CGCG_T
Cuts at: 3893 3893
Size: 4460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 418 3131 3315 418
Size: 2713 184 1563
Fragments arranged by size:
2713 1563 184
MscI TGG'CCA
Cuts at: 136 2221 136
Size: 2085 2375
MslI CAynn'nnrTG
Cuts at: 168 692 1367 2449 2576 168
Size: 524 675 1082 127 2052
Fragments arranged by size:
2052 1082 675 524 127
MspA1I CmG'CkG
Cuts at: 1571 1885 2245 3258 3503 1571
Size: 314 360 1013 245 2528
Fragments arranged by size:
2528 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2641 2641
Size: 4460
NarI GG'CG_CC
Cuts at: 1891 2139 1891
Size: 248 4212
NgoMIV G'CCGG_C
Cuts at: 2639 2639
Size: 4460
NspI r_CATG'y
Cuts at: 230 345 847 1513 2013 2920 230
Size: 115 502 666 500 907 1770
Fragments arranged by size:
1770 907 666 502 500 115
PciI A'CATG_T
Cuts at: 2916 2916
Size: 4460
PfoI T'CCnGG_A
Cuts at: 308 559 577 774 308
Size: 251 18 197 3994
Fragments arranged by size:
3994 251 197 18
PmeI GTTT'AAAC
Cuts at: 1459 1459
Size: 4460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4428
PpuMI rG'GwC_Cy
Cuts at: 612 612
Size: 4460
PsiI TTA'TAA
Cuts at: 466 466
Size: 4460
PstI C_TGCA'G
Cuts at: 1507 2192 1507
Size: 685 3775
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4460
PvuII CAG'CTG
Cuts at: 1885 2245 1885
Size: 360 4100
RsrII CG'GwC_CG
Cuts at: 2655 2655
Size: 4460
SacI G_AGCT'C
Cuts at: 1474 1474
Size: 4460
SalI G'TCGA_C
Cuts at: 1497 1497
Size: 4460
SapI GCTCTTCn'nnn_
Cuts at: 2483 2693 2483
Size: 210 4250
SbfI CC_TGCA'GG
Cuts at: 1507 1507
Size: 4460
ScaI AGT'ACT
Cuts at: 3977 3977
Size: 4460
SfcI C'TryA_G
Cuts at: 33 1503 2188 3181 3372 33
Size: 1470 685 993 191 1121
Fragments arranged by size:
1470 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1892 2140 1892
Size: 248 4212
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
SmaI CCC'GGG
Cuts at: 1482 1482
Size: 4460
SmlI C'TyrA_G
Cuts at: 205 671 1200 1615 2530 3022 3284 205
Size: 466 529 415 915 492 262 1381
Fragments arranged by size:
1381 915 529 492 466 415 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4460
SphI G_CATG'C
Cuts at: 345 1513 2013 345
Size: 1168 500 2792
Fragments arranged by size:
2792 1168 500
SspI AAT'ATT
Cuts at: 473 732 785 473
Size: 259 53 4148
Fragments arranged by size:
4148 259 53
StyI C'CwwG_G
Cuts at: 1594 1948 1594
Size: 354 4106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 172 733 2043 2176 2635 2844 172
Size: 561 1310 133 459 209 1788
Fragments arranged by size:
1788 1310 561 459 209 133
TatI w'GTAC_w
Cuts at: 3975 3975
Size: 4460
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 794 826 3977 4009 794
Size: 32 3151 32 1245
Fragments arranged by size:
3151 1245 32 32
Tth111I GACn'n_nGTC
Cuts at: 2257 2257
Size: 4460
XbaI T'CTAG_A
Cuts at: 858 1005 1491 858
Size: 147 486 3827
Fragments arranged by size:
3827 486 147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1955 1955
Size: 4460
XmaI C'CCGG_G
Cuts at: 1480 1480
Size: 4460
XmnI GAAnn'nnTTC
Cuts at: 1091 1322 1091
Size: 231 4229
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BfrBI BglI BmgBI BmtI BpmI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI