Restriction map for
pF1KE2444
(Circular) (Six-base) MAPSORT of: pF1KE2444.seq Check: 6142 from: 1 to: 4436
pF1KE2444 4436 bp
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 599 599
Size: 4436
AccI GT'mk_AC
Cuts at: 1474 1474
Size: 4436
Acc65I G'GTAC_C
Cuts at: 1452 1452
Size: 4436
AclI AA'CG_TT
Cuts at: 4276 4276
Size: 4436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2261 2693 3440 3708 2261
Size: 432 747 268 2989
Fragments arranged by size:
2989 747 432 268
AflIII A'CryG_T
Cuts at: 464 1788 2892 3869 464
Size: 1324 1104 977 1031
Fragments arranged by size:
1324 1104 1031 977
AgeI A'CCGG_T
Cuts at: 2824 2824
Size: 4436
AlwNI CAG_nnn'CTG
Cuts at: 1272 1365 1741 3308 1272
Size: 93 376 1567 2400
Fragments arranged by size:
2400 1567 376 93
ApaLI G'TGCA_C
Cuts at: 3206 3206
Size: 4436
ApoI r'AATT_y
Cuts at: 232 349 476 546 741 1325 1440 3789 4045
Size: 117 127 70 195 584 115 2349 256
Cuts at: 4045 232
Size: 623
Fragments arranged by size:
2349 623 584 256 195 127 117 115
70
AseI AT'TA_AT
Cuts at: 20 20
Size: 4436
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
AvaI C'yCGr_G
Cuts at: 1456 1456
Size: 4436
BamHI G'GATC_C
Cuts at: 1461 1461
Size: 4436
BanI G'GyrC_C
Cuts at: 1452 1866 2114 2149 1452
Size: 414 248 35 3739
Fragments arranged by size:
3739 414 248 35
BanII G_rGCy'C
Cuts at: 1379 1450 2480 1379
Size: 71 1030 3335
Fragments arranged by size:
3335 1030 71
BbeI G_GCGC'C
Cuts at: 1870 2118 1870
Size: 248 4188
BbsI GAAGACnn'nnnn_
Cuts at: 623 758 623
Size: 135 4301
BciVI GTATCCnnnnn_n'
Cuts at: 2334 3095 4436 2334
Size: 761 1341 2334
Fragments arranged by size:
2334 1341 761
BclI T'GATC_A
Cuts at: 1956 1956
Size: 4436
BglII A'GATC_T
Cuts at: 188 1951 3988 188
Size: 1763 2037 636
Fragments arranged by size:
2037 1763 636
BlpI GC'TnA_GC
Cuts at: 805 1548 805
Size: 743 3693
Bme1580I G_kGCm'C
Cuts at: 2061 2154 3210 2061
Size: 93 1056 3287
Fragments arranged by size:
3287 1056 93
BmgBI CAC'GTC
Cuts at: 144 144
Size: 4436
BmrI ACTGGGnnnn_n'
Cuts at: 1071 1648 1729 2064 4208 1071
Size: 577 81 335 2144 1299
Fragments arranged by size:
2144 1299 577 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2803 2803
Size: 4436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1612 2491 2983 3281 1612
Size: 879 492 298 2767
Fragments arranged by size:
2767 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4140 4140
Size: 4436
BsaAI yAC'GTr
Cuts at: 1789 1789
Size: 4436
BsaBI GATnn'nnATC
Cuts at: 6 629 1950 3987 6
Size: 623 1321 2037 455
Fragments arranged by size:
2037 1321 623 455
BsaHI Gr'CG_yC
Cuts at: 124 1867 2115 2817 4313 124
Size: 1743 248 702 1496 247
Fragments arranged by size:
1743 1496 702 248 247
BsaWI w'CCGG_w
Cuts at: 1625 2146 2824 3098 3245 3656 1625
Size: 521 678 274 147 411 2405
Fragments arranged by size:
2405 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1378 1861 2619 3196 3692 1378
Size: 483 758 577 496 2122
Fragments arranged by size:
2122 758 577 496 483
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1274 1274
Size: 4436
BsiEI CG_ry'CG
Cuts at: 104 1978 2024 3232 104
Size: 1874 46 1208 1308
Fragments arranged by size:
1874 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1450 2228 2418 3210 1450
Size: 778 190 792 2676
Fragments arranged by size:
2676 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 131 131
Size: 4436
Bsp1286I G_dGCh'C
Cuts at: 1379 1450 2061 2154 2228 2418 2480 3210 1379
Size: 71 611 93 74 190 62 730 2605
Fragments arranged by size:
2605 730 611 190 93 74 71 62
BspEI T'CCGG_A
Cuts at: 3656 3656
Size: 4436
BspHI T'CATG_A
Cuts at: 480 1125 3612 480
Size: 645 2487 1304
Fragments arranged by size:
2487 1304 645
BspMI ACCTGCnnnn'nnnn_
Cuts at: 599 1486 1700 2002 2383 599
Size: 887 214 302 381 2652
Fragments arranged by size:
2652 887 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2728 2782 4266 4431 50
Size: 2678 54 1484 165 55
Fragments arranged by size:
2678 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 211 572 799 1145 2348 211
Size: 361 227 346 1203 2299
Fragments arranged by size:
2299 1203 361 346 227
BsrFI r'CCGG_y
Cuts at: 2434 2615 2824 2434
Size: 181 209 4046
Fragments arranged by size:
4046 209 181
BssSI C'ACGA_G
Cuts at: 2707 3065 2707
Size: 358 4078
BstAPI GCAn_nnn'nTGC
Cuts at: 1813 1813
Size: 4436
BstBI TT'CG_AA
Cuts at: 2797 2797
Size: 4436
BstYI r'GATC_y
Cuts at: 1 188 1461 1928 1951 2286 2532 3544 3630
Size: 187 1273 467 23 335 246 1012 86
Cuts at: 3630 3642 3988 1
Size: 12 346 449
Fragments arranged by size:
1273 1012 467 449 346 335 246 187
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1145 1145
Size: 4436
BtgI C'CryG_G
Cuts at: 107 442 964 107
Size: 335 522 3579
Fragments arranged by size:
3579 522 335
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2386 2567 2730 2386
Size: 181 163 4092
Fragments arranged by size:
4092 181 163
BtsI GCAGTG_nn'
Cuts at: 1679 1679
Size: 4436
ClaI AT'CG_AT
Cuts at: 509 509
Size: 4436
DraI TTT'AAA
Cuts at: 404 528 654 1435 404
Size: 124 126 781 3405
Fragments arranged by size:
3405 781 126 124
DrdI GACnn_nn'nnGTC
Cuts at: 2142 3000 2142
Size: 858 3578
EaeI y'GGCC_r
Cuts at: 2021 2195 2586 2613 3958 2021
Size: 174 391 27 1345 2499
Fragments arranged by size:
2499 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2021 2021
Size: 4436
EarI CTCTTCn'nnn_
Cuts at: 1061 2459 2669 1061
Size: 1398 210 2828
Fragments arranged by size:
2828 1398 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2644 2954 3100 4246 2644
Size: 310 146 1146 2834
Fragments arranged by size:
2834 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1448 1448
Size: 4436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2261 2693 3440 3708 2261
Size: 432 747 268 2989
Fragments arranged by size:
2989 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1349 1349
Size: 4436
EcoO109I rG'GnC_Cy
Cuts at: 1575 1575
Size: 4436
EcoRI G'AATT_C
Cuts at: 476 1440 476
Size: 964 3472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 4436
FspI TGC'GCA
Cuts at: 2217 2217
Size: 4436
HaeII r_GCGC'y
Cuts at: 1870 2118 3140 4119 1870
Size: 248 1022 979 2187
Fragments arranged by size:
2187 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 133 165 406 438 1107 1139 2278 2308 2310
Size: 32 241 32 669 32 1139 30 2
Cuts at: 2310 2340 133
Size: 30 2229
Fragments arranged by size:
2229 1139 669 241 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1226 1475 1226
Size: 249 4187
HindIII A'AGCT_T
Cuts at: 833 833
Size: 4436
KasI G'GCGC_C
Cuts at: 1866 2114 1866
Size: 248 4188
KpnI G_GTAC'C
Cuts at: 1456 1456
Size: 4436
MfeI C'AATT_G
Cuts at: 598 810 598
Size: 212 4224
MluI A'CGCG_T
Cuts at: 3869 3869
Size: 4436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3107 3291 3107
Size: 184 4252
MscI TGG'CCA
Cuts at: 2197 2197
Size: 4436
MslI CAynn'nnrTG
Cuts at: 227 947 2425 2552 227
Size: 720 1478 127 2111
Fragments arranged by size:
2111 1478 720 127
MspA1I CmG'CkG
Cuts at: 636 1382 1547 1861 2221 3234 3479 636
Size: 746 165 314 360 1013 245 1593
Fragments arranged by size:
1593 1013 746 360 314 245 165
NaeI GCC'GGC
Cuts at: 2617 2617
Size: 4436
NarI GG'CG_CC
Cuts at: 1867 2115 1867
Size: 248 4188
NcoI C'CATG_G
Cuts at: 107 964 107
Size: 857 3579
NgoMIV G'CCGG_C
Cuts at: 2615 2615
Size: 4436
NspI r_CATG'y
Cuts at: 468 1489 1989 2896 468
Size: 1021 500 907 2008
Fragments arranged by size:
2008 1021 907 500
PciI A'CATG_T
Cuts at: 464 2892 464
Size: 2428 2008
PflMI CCAn_nnn'nTGG
Cuts at: 1385 1385
Size: 4436
PmeI GTTT'AAAC
Cuts at: 1435 1435
Size: 4436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 3575
Size: 32 4404
PstI C_TGCA'G
Cuts at: 665 1343 1483 2168 665
Size: 678 140 685 2933
Fragments arranged by size:
2933 685 678 140
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4436
PvuII CAG'CTG
Cuts at: 636 1382 1861 2221 636
Size: 746 479 360 2851
Fragments arranged by size:
2851 746 479 360
RsrII CG'GwC_CG
Cuts at: 2631 2631
Size: 4436
SacI G_AGCT'C
Cuts at: 1450 1450
Size: 4436
SalI G'TCGA_C
Cuts at: 1473 1473
Size: 4436
SapI GCTCTTCn'nnn_
Cuts at: 2459 2669 2459
Size: 210 4226
SbfI CC_TGCA'GG
Cuts at: 1483 1483
Size: 4436
ScaI AGT'ACT
Cuts at: 1132 3953 1132
Size: 2821 1615
SfcI C'TryA_G
Cuts at: 33 661 1174 1339 1479 2164 3157 3348 33
Size: 628 513 165 140 685 993 191 1121
Fragments arranged by size:
1121 993 685 628 513 191 165 140
SfoI GGC'GCC
Cuts at: 1868 2116 1868
Size: 248 4188
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
SmaI CCC'GGG
Cuts at: 1458 1458
Size: 4436
SmlI C'TyrA_G
Cuts at: 1591 2506 2998 3260 1591
Size: 915 492 262 2767
Fragments arranged by size:
2767 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4436
SphI G_CATG'C
Cuts at: 1489 1989 1489
Size: 500 3936
SspI AAT'ATT
Cuts at: 899 1409 899
Size: 510 3926
StyI C'CwwG_G
Cuts at: 107 338 964 1570 1924 107
Size: 231 626 606 354 2619
Fragments arranged by size:
2619 626 606 354 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2019 2152 2611 2820 2019
Size: 133 459 209 3635
Fragments arranged by size:
3635 459 209 133
TatI w'GTAC_w
Cuts at: 583 735 1130 3951 583
Size: 152 395 2821 1068
Fragments arranged by size:
2821 1068 395 152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 128 160 3953 3985 128
Size: 32 3793 32 579
Fragments arranged by size:
3793 579 32 32
Tth111I GACn'n_nGTC
Cuts at: 2233 2233
Size: 4436
XbaI T'CTAG_A
Cuts at: 1467 1467
Size: 4436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1931 1931
Size: 4436
XmaI C'CCGG_G
Cuts at: 1456 1456
Size: 4436
XmnI GAAnn'nnTTC
Cuts at: 1279 1279
Size: 4436
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I CspCI DraIII EcoRV FseI FspAI
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI