Restriction map for
pF1KE2434
(Circular) (Six-base) MAPSORT of: pF1KE2434.seq Check: 3839 from: 1 to: 3986
pF1KE2434 3986 bp
With 182 enzymes: SgfI *
December 4, 2012 11:59 ..
AccI GT'mk_AC
Cuts at: 282 1024 282
Size: 742 3244
Acc65I G'GTAC_C
Cuts at: 1002 1002
Size: 3986
AclI AA'CG_TT
Cuts at: 3826 3826
Size: 3986
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 1811 2243 2990 3258 521
Size: 1290 432 747 268 1249
Fragments arranged by size:
1290 1249 747 432 268
AflIII A'CryG_T
Cuts at: 1338 2442 3419 1338
Size: 1104 977 1905
Fragments arranged by size:
1905 1104 977
AgeI A'CCGG_T
Cuts at: 2374 2374
Size: 3986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 794 794
Size: 3986
AlwNI CAG_nnn'CTG
Cuts at: 1291 2858 1291
Size: 1567 2419
ApaLI G'TGCA_C
Cuts at: 2756 2756
Size: 3986
ApoI r'AATT_y
Cuts at: 364 470 560 621 990 3339 3595 364
Size: 106 90 61 369 2349 256 755
Fragments arranged by size:
2349 755 369 256 106 90 61
AseI AT'TA_AT
Cuts at: 20 20
Size: 3986
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
AvaI C'yCGr_G
Cuts at: 1006 1006
Size: 3986
BamHI G'GATC_C
Cuts at: 1011 1011
Size: 3986
BanI G'GyrC_C
Cuts at: 1002 1416 1664 1699 1002
Size: 414 248 35 3289
Fragments arranged by size:
3289 414 248 35
BanII G_rGCy'C
Cuts at: 1000 2030 1000
Size: 1030 2956
BbeI G_GCGC'C
Cuts at: 1420 1668 1420
Size: 248 3738
BbsI GAAGACnn'nnnn_
Cuts at: 123 123
Size: 3986
BbvCI CC'TCA_GC
Cuts at: 460 460
Size: 3986
BciVI GTATCCnnnnn_n'
Cuts at: 1884 2645 3986 1884
Size: 761 1341 1884
Fragments arranged by size:
1884 1341 761
BclI T'GATC_A
Cuts at: 140 1506 140
Size: 1366 2620
BfrBI ATG'CAT
Cuts at: 921 921
Size: 3986
BglII A'GATC_T
Cuts at: 1501 3538 1501
Size: 2037 1949
BlpI GC'TnA_GC
Cuts at: 940 1098 940
Size: 158 3828
Bme1580I G_kGCm'C
Cuts at: 1611 1704 2760 1611
Size: 93 1056 2837
Fragments arranged by size:
2837 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 508 1198 1279 1614 3758 508
Size: 690 81 335 2144 736
Fragments arranged by size:
2144 736 690 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 3986
Bpu10I CC'TnA_GC
Cuts at: 460 460
Size: 3986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 1162 2041 2533 2831 175
Size: 987 879 492 298 1330
Fragments arranged by size:
1330 987 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 432 3690 432
Size: 3258 728
BsaAI yAC'GTr
Cuts at: 1339 1339
Size: 3986
BsaBI GATnn'nnATC
Cuts at: 6 115 253 1500 3537 6
Size: 109 138 1247 2037 455
Fragments arranged by size:
2037 1247 455 138 109
BsaHI Gr'CG_yC
Cuts at: 1417 1665 2367 3863 1417
Size: 248 702 1496 1540
Fragments arranged by size:
1540 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1175 1696 2374 2648 2795 3206 1175
Size: 521 678 274 147 411 1955
Fragments arranged by size:
1955 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 201 1411 2169 2746 3242 201
Size: 1210 758 577 496 945
Fragments arranged by size:
1210 945 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 196
Size: 3986
BsiEI CG_ry'CG
Cuts at: 104 1528 1574 2782 104
Size: 1424 46 1208 1308
Fragments arranged by size:
1424 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1000 1778 1968 2760 1000
Size: 778 190 792 2226
Fragments arranged by size:
2226 792 778 190
BsmI GAATG_Cn'
Cuts at: 921 921
Size: 3986
Bsp1286I G_dGCh'C
Cuts at: 1000 1611 1704 1778 1968 2030 2760 1000
Size: 611 93 74 190 62 730 2226
Fragments arranged by size:
2226 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3206 3206
Size: 3986
BspHI T'CATG_A
Cuts at: 137 3162 137
Size: 3025 961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1036 1250 1552 1933 1036
Size: 214 302 381 3089
Fragments arranged by size:
3089 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2278 2332 3816 3981 50
Size: 2228 54 1484 165 55
Fragments arranged by size:
2228 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1898 1898
Size: 3986
BsrFI r'CCGG_y
Cuts at: 1984 2165 2374 1984
Size: 181 209 3596
Fragments arranged by size:
3596 209 181
BssSI C'ACGA_G
Cuts at: 2257 2615 2257
Size: 358 3628
BstAPI GCAn_nnn'nTGC
Cuts at: 1363 1363
Size: 3986
BstBI TT'CG_AA
Cuts at: 2347 2347
Size: 3986
BstYI r'GATC_y
Cuts at: 1 1011 1478 1501 1836 2082 3094 3180 3192
Size: 1010 467 23 335 246 1012 86 12
Cuts at: 3192 3538 1
Size: 346 449
Fragments arranged by size:
1012 1010 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 784 784
Size: 3986
BtgI C'CryG_G
Cuts at: 575 575
Size: 3986
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1936 2117 2280 1936
Size: 181 163 3642
Fragments arranged by size:
3642 181 163
BtsI GCAGTG_nn'
Cuts at: 203 747 776 1229 203
Size: 544 29 453 2960
Fragments arranged by size:
2960 544 453 29
DraI TTT'AAA
Cuts at: 985 985
Size: 3986
DrdI GACnn_nn'nnGTC
Cuts at: 1692 2550 1692
Size: 858 3128
EaeI y'GGCC_r
Cuts at: 1571 1745 2136 2163 3508 1571
Size: 174 391 27 1345 2049
Fragments arranged by size:
2049 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1571 1571
Size: 3986
EarI CTCTTCn'nnn_
Cuts at: 435 2009 2219 435
Size: 1574 210 2202
Fragments arranged by size:
2202 1574 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2194 2504 2650 3796 2194
Size: 310 146 1146 2384
Fragments arranged by size:
2384 1146 310 146
EcoICRI GAG'CTC
Cuts at: 998 998
Size: 3986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 1811 2243 2990 3258 521
Size: 1290 432 747 268 1249
Fragments arranged by size:
1290 1249 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 788 1125 788
Size: 337 3649
EcoRI G'AATT_C
Cuts at: 990 990
Size: 3986
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3986
FspI TGC'GCA
Cuts at: 1767 1767
Size: 3986
HaeII r_GCGC'y
Cuts at: 1420 1668 2690 3669 1420
Size: 248 1022 979 1737
Fragments arranged by size:
1737 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 717 749 1828 1858 1860 1890 717
Size: 32 1079 30 2 30 2813
Fragments arranged by size:
2813 1079 32 30 30 2
HincII GTy'rAC
Cuts at: 1025 1025
Size: 3986
HindIII A'AGCT_T
Cuts at: 893 893
Size: 3986
KasI G'GCGC_C
Cuts at: 1416 1664 1416
Size: 248 3738
KpnI G_GTAC'C
Cuts at: 1006 1006
Size: 3986
MluI A'CGCG_T
Cuts at: 3419 3419
Size: 3986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 144 2657 2841 144
Size: 2513 184 1289
Fragments arranged by size:
2513 1289 184
MscI TGG'CCA
Cuts at: 1747 1747
Size: 3986
MslI CAynn'nnrTG
Cuts at: 148 916 1975 2102 148
Size: 768 1059 127 2032
Fragments arranged by size:
2032 1059 768 127
MspA1I CmG'CkG
Cuts at: 1097 1411 1771 2784 3029 1097
Size: 314 360 1013 245 2054
Fragments arranged by size:
2054 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2167 2167
Size: 3986
NarI GG'CG_CC
Cuts at: 1417 1665 1417
Size: 248 3738
NcoI C'CATG_G
Cuts at: 575 575
Size: 3986
NgoMIV G'CCGG_C
Cuts at: 2165 2165
Size: 3986
NsiI A_TGCA'T
Cuts at: 923 923
Size: 3986
NspI r_CATG'y
Cuts at: 1039 1539 2446 1039
Size: 500 907 2579
Fragments arranged by size:
2579 907 500
PciI A'CATG_T
Cuts at: 2442 2442
Size: 3986
PmeI GTTT'AAAC
Cuts at: 985 985
Size: 3986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 3954
PpuMI rG'GwC_Cy
Cuts at: 788 788
Size: 3986
PstI C_TGCA'G
Cuts at: 1033 1718 1033
Size: 685 3301
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3986
PvuII CAG'CTG
Cuts at: 1411 1771 1411
Size: 360 3626
RsrII CG'GwC_CG
Cuts at: 2181 2181
Size: 3986
SacI G_AGCT'C
Cuts at: 1000 1000
Size: 3986
SalI G'TCGA_C
Cuts at: 1023 1023
Size: 3986
SapI GCTCTTCn'nnn_
Cuts at: 2009 2219 2009
Size: 210 3776
SbfI CC_TGCA'GG
Cuts at: 1033 1033
Size: 3986
ScaI AGT'ACT
Cuts at: 3503 3503
Size: 3986
SfcI C'TryA_G
Cuts at: 33 283 1029 1714 2707 2898 33
Size: 250 746 685 993 191 1121
Fragments arranged by size:
1121 993 746 685 250 191
SfoI GGC'GCC
Cuts at: 1418 1666 1418
Size: 248 3738
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
SmaI CCC'GGG
Cuts at: 1008 1008
Size: 3986
SmlI C'TyrA_G
Cuts at: 154 1141 2056 2548 2810 154
Size: 987 915 492 262 1330
Fragments arranged by size:
1330 987 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3986
SphI G_CATG'C
Cuts at: 1039 1539 1039
Size: 500 3486
StyI C'CwwG_G
Cuts at: 575 1120 1474 575
Size: 545 354 3087
Fragments arranged by size:
3087 545 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 454 1569 1702 2161 2370 454
Size: 1115 133 459 209 2070
Fragments arranged by size:
2070 1115 459 209 133
TatI w'GTAC_w
Cuts at: 336 3501 336
Size: 3165 821
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 3954
Tth111I GACn'n_nGTC
Cuts at: 1783 1783
Size: 3986
XbaI T'CTAG_A
Cuts at: 1017 1017
Size: 3986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 702 1481 702
Size: 779 3207
XmaI C'CCGG_G
Cuts at: 1006 1006
Size: 3986
XmnI GAAnn'nnTTC
Cuts at: 623 623
Size: 3986
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BglI BmgBI BmtI BpmI
BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI