Restriction map for
pF1KE2426
(Circular) (Six-base) MAPSORT of: pF1KE2426.seq Check: 9038 from: 1 to: 4964
pF1KE2426 4964 bp
With 182 enzymes: SgfI *
November 16, 2012 10:46 ..
AccI GT'mk_AC
Cuts at: 689 1737 2002 689
Size: 1048 265 3651
Fragments arranged by size:
3651 1048 265
Acc65I G'GTAC_C
Cuts at: 1920 1980 1920
Size: 60 4904
AclI AA'CG_TT
Cuts at: 4804 4804
Size: 4964
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1946 2789 3221 3968 4236 952
Size: 994 843 432 747 268 1680
Fragments arranged by size:
1680 994 843 747 432 268
AflII C'TTAA_G
Cuts at: 1722 1722
Size: 4964
AflIII A'CryG_T
Cuts at: 1879 2316 3420 4397 1879
Size: 437 1104 977 2446
Fragments arranged by size:
2446 1104 977 437
AgeI A'CCGG_T
Cuts at: 3352 3352
Size: 4964
AhdI GACnn_n'nnGTC
Cuts at: 1662 1662
Size: 4964
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 165 864 165
Size: 699 4265
AlwNI CAG_nnn'CTG
Cuts at: 340 709 2269 3836 340
Size: 369 1560 1567 1468
Fragments arranged by size:
1567 1560 1468 369
ApaI G_GGCC'C
Cuts at: 1775 1775
Size: 4964
ApaLI G'TGCA_C
Cuts at: 782 3734 782
Size: 2952 2012
ApoI r'AATT_y
Cuts at: 551 572 1328 1807 1968 4317 4573 551
Size: 21 756 479 161 2349 256 942
Fragments arranged by size:
2349 942 756 479 256 161 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 4964
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4964
AvaI C'yCGr_G
Cuts at: 257 1774 1984 257
Size: 1517 210 3237
Fragments arranged by size:
3237 1517 210
BamHI G'GATC_C
Cuts at: 1989 1989
Size: 4964
BanI G'GyrC_C
Cuts at: 114 1920 1980 2394 2642 2677 114
Size: 1806 60 414 248 35 2401
Fragments arranged by size:
2401 1806 414 248 60 35
BanII G_rGCy'C
Cuts at: 350 441 516 1203 1775 1978 3008 350
Size: 91 75 687 572 203 1030 2306
Fragments arranged by size:
2306 1030 687 572 203 91 75
BbeI G_GCGC'C
Cuts at: 2398 2646 2398
Size: 248 4716
BbsI GAAGACnn'nnnn_
Cuts at: 200 545 200
Size: 345 4619
BbvCI CC'TCA_GC
Cuts at: 710 710
Size: 4964
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4930
BciVI GTATCCnnnnn_n'
Cuts at: 761 1637 2862 3623 4964 761
Size: 876 1225 761 1341 761
Fragments arranged by size:
1341 1225 876 761 761
BclI T'GATC_A
Cuts at: 2484 2484
Size: 4964
BglII A'GATC_T
Cuts at: 2479 4516 2479
Size: 2037 2927
BlpI GC'TnA_GC
Cuts at: 2076 2076
Size: 4964
Bme1580I G_kGCm'C
Cuts at: 786 1775 2589 2682 3738 786
Size: 989 814 93 1056 2012
Fragments arranged by size:
2012 1056 989 814 93
BmrI ACTGGGnnnn_n'
Cuts at: 1501 1923 2176 2257 2592 4736 1501
Size: 422 253 81 335 2144 1729
Fragments arranged by size:
2144 1729 422 335 253 81
BmtI G_CTAG'C
Cuts at: 124 124
Size: 4964
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3331 3331
Size: 4964
Bpu10I CC'TnA_GC
Cuts at: 710 710
Size: 4964
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1579 2140 3019 3511 3809 1579
Size: 561 879 492 298 2734
Fragments arranged by size:
2734 879 561 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4668 4668
Size: 4964
BsaAI yAC'GTr
Cuts at: 2317 2317
Size: 4964
BsaBI GATnn'nnATC
Cuts at: 6 2478 4515 6
Size: 2472 2037 455
Fragments arranged by size:
2472 2037 455
BsaHI Gr'CG_yC
Cuts at: 2395 2643 3345 4841 2395
Size: 248 702 1496 2518
Fragments arranged by size:
2518 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2153 2674 3352 3626 3773 4184 2153
Size: 521 678 274 147 411 2933
Fragments arranged by size:
2933 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1949 1949
Size: 4964
BseYI C'CCAG_C
Cuts at: 2389 3147 3724 4220 2389
Size: 758 577 496 3133
Fragments arranged by size:
3133 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 353
Size: 4964
BsiEI CG_ry'CG
Cuts at: 104 2506 2552 3760 104
Size: 2402 46 1208 1308
Fragments arranged by size:
2402 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 441 786 1203 1978 2756 2946 3738 441
Size: 345 417 775 778 190 792 1667
Fragments arranged by size:
1667 792 778 775 417 345 190
BsmI GAATG_Cn'
Cuts at: 1151 1151
Size: 4964
Bsp1286I G_dGCh'C
Cuts at: 350 441 516 786 1203 1775 1978 2589 2682
Size: 91 75 270 417 572 203 611 93
Cuts at: 2682 2756 2946 3008 3738 350
Size: 74 190 62 730 1576
Fragments arranged by size:
1576 730 611 572 417 270 203 190
93 91 75 74 62
BspEI T'CCGG_A
Cuts at: 4184 4184
Size: 4964
BspHI T'CATG_A
Cuts at: 723 1692 4140 723
Size: 969 2448 1547
Fragments arranged by size:
2448 1547 969
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1950 2014 2228 2530 2911 1950
Size: 64 214 302 381 4003
Fragments arranged by size:
4003 381 302 214 64
BsrBI CCG'CTC
Cuts at: 50 3256 3310 4794 4959 50
Size: 3206 54 1484 165 55
Fragments arranged by size:
3206 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1322 2876 1322
Size: 1554 3410
BsrFI r'CCGG_y
Cuts at: 1707 2962 3143 3352 1707
Size: 1255 181 209 3319
Fragments arranged by size:
3319 1255 209 181
BsrGI T'GTAC_A
Cuts at: 1866 1866
Size: 4964
BssSI C'ACGA_G
Cuts at: 3235 3593 3235
Size: 358 4606
BstAPI GCAn_nnn'nTGC
Cuts at: 340 2341 340
Size: 2001 2963
BstBI TT'CG_AA
Cuts at: 3325 3325
Size: 4964
BstYI r'GATC_y
Cuts at: 1 1024 1893 1989 2456 2479 2814 3060 4072
Size: 1023 869 96 467 23 335 246 1012
Cuts at: 4072 4158 4170 4516 1
Size: 86 12 346 449
Fragments arranged by size:
1023 1012 869 467 449 346 335 246
96 86 23 12
BstZ17I GTA'TAC
Cuts at: 690 1738 690
Size: 1048 3916
BtgI C'CryG_G
Cuts at: 852 1482 1555 852
Size: 630 73 4261
Fragments arranged by size:
4261 630 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2914 3095 3258 2914
Size: 181 163 4620
Fragments arranged by size:
4620 181 163
BtsI GCAGTG_nn'
Cuts at: 2207 2207
Size: 4964
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1194 1229 1194
Size: 35 4929
DraI TTT'AAA
Cuts at: 480 1963 480
Size: 1483 3481
DrdI GACnn_nn'nnGTC
Cuts at: 2670 3528 2670
Size: 858 4106
EaeI y'GGCC_r
Cuts at: 1156 2549 2723 3114 3141 4486 1156
Size: 1393 174 391 27 1345 1634
Fragments arranged by size:
1634 1393 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2549 2549
Size: 4964
EarI CTCTTCn'nnn_
Cuts at: 493 2987 3197 493
Size: 2494 210 2260
Fragments arranged by size:
2494 2260 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3172 3482 3628 4774 3172
Size: 310 146 1146 3362
Fragments arranged by size:
3362 1146 310 146
EcoICRI GAG'CTC
Cuts at: 439 1201 1976 439
Size: 762 775 3427
Fragments arranged by size:
3427 775 762
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1946 2789 3221 3968 4236 952
Size: 994 843 432 747 268 1680
Fragments arranged by size:
1680 994 843 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 337 714 1939 2103 337
Size: 377 1225 164 3198
Fragments arranged by size:
3198 1225 377 164
EcoRI G'AATT_C
Cuts at: 572 1968 572
Size: 1396 3568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 224 2983 224
Size: 2759 2205
FspI TGC'GCA
Cuts at: 2745 2745
Size: 4964
HaeII r_GCGC'y
Cuts at: 2398 2646 3668 4647 2398
Size: 248 1022 979 2715
Fragments arranged by size:
2715 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 423 455 966 998 1016 1048 1648 1680 1839
Size: 32 511 32 18 32 600 32 159
Cuts at: 1839 1871 2806 2836 2838 2868 423
Size: 32 935 30 2 30 2519
Fragments arranged by size:
2519 935 600 511 159 32 32 32
32 32 30 30 18 2
HincII GTy'rAC
Cuts at: 2003 2003
Size: 4964
KasI G'GCGC_C
Cuts at: 2394 2642 2394
Size: 248 4716
KpnI G_GTAC'C
Cuts at: 1924 1984 1924
Size: 60 4904
MluI A'CGCG_T
Cuts at: 4397 4397
Size: 4964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3635 3819 3635
Size: 184 4780
MscI TGG'CCA
Cuts at: 2725 2725
Size: 4964
MslI CAynn'nnrTG
Cuts at: 112 147 403 728 1016 1043 1315 1437 1697
Size: 35 256 325 288 27 272 122 260
Cuts at: 1697 2953 3080 112
Size: 1256 127 1996
Fragments arranged by size:
1996 1256 325 288 272 260 256 127
122 35 27
MspA1I CmG'CkG
Cuts at: 358 706 2075 2389 2749 3762 4007 358
Size: 348 1369 314 360 1013 245 1315
Fragments arranged by size:
1369 1315 1013 360 348 314 245
NaeI GCC'GGC
Cuts at: 1709 3145 1709
Size: 1436 3528
NarI GG'CG_CC
Cuts at: 2395 2643 2395
Size: 248 4716
NcoI C'CATG_G
Cuts at: 852 852
Size: 4964
NgoMIV G'CCGG_C
Cuts at: 1707 3143 1707
Size: 1436 3528
NheI G'CTAG_C
Cuts at: 120 120
Size: 4964
NspI r_CATG'y
Cuts at: 823 1883 2017 2517 3424 823
Size: 1060 134 500 907 2363
Fragments arranged by size:
2363 1060 907 500 134
PciI A'CATG_T
Cuts at: 1879 3420 1879
Size: 1541 3423
PmeI GTTT'AAAC
Cuts at: 1963 1963
Size: 4964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4103 4135 4103
Size: 32 4932
PpuMI rG'GwC_Cy
Cuts at: 714 1939 714
Size: 1225 3739
PspOMI G'GGCC_C
Cuts at: 1771 1771
Size: 4964
PstI C_TGCA'G
Cuts at: 345 357 1797 2011 2696 345
Size: 12 1440 214 685 2613
Fragments arranged by size:
2613 1440 685 214 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4964
PvuII CAG'CTG
Cuts at: 358 706 2389 2749 358
Size: 348 1683 360 2573
Fragments arranged by size:
2573 1683 360 348
RsrII CG'GwC_CG
Cuts at: 3159 3159
Size: 4964
SacI G_AGCT'C
Cuts at: 441 1203 1978 441
Size: 762 775 3427
Fragments arranged by size:
3427 775 762
SalI G'TCGA_C
Cuts at: 2001 2001
Size: 4964
SapI GCTCTTCn'nnn_
Cuts at: 2987 3197 2987
Size: 210 4754
SbfI CC_TGCA'GG
Cuts at: 345 1797 2011 345
Size: 1452 214 3298
Fragments arranged by size:
3298 1452 214
ScaI AGT'ACT
Cuts at: 1490 4481 1490
Size: 2991 1973
SfcI C'TryA_G
Cuts at: 33 341 353 1793 2007 2692 3685 3876 33
Size: 308 12 1440 214 685 993 191 1121
Fragments arranged by size:
1440 1121 993 685 308 214 191 12
SfoI GGC'GCC
Cuts at: 2396 2644 2396
Size: 248 4716
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4964
SmaI CCC'GGG
Cuts at: 259 1986 259
Size: 1727 3237
SmlI C'TyrA_G
Cuts at: 1594 1722 2119 3034 3526 3788 1594
Size: 128 397 915 492 262 2770
Fragments arranged by size:
2770 915 492 397 262 128
SpeI A'CTAG_T
Cuts at: 67 1363 67
Size: 1296 3668
SphI G_CATG'C
Cuts at: 2017 2517 2017
Size: 500 4464
SspI AAT'ATT
Cuts at: 1908 1908
Size: 4964
StyI C'CwwG_G
Cuts at: 675 852 1001 1163 1604 2098 2452 675
Size: 177 149 162 441 494 354 3187
Fragments arranged by size:
3187 494 441 354 177 162 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1483 1905 2547 2680 3139 3348 1483
Size: 422 642 133 459 209 3099
Fragments arranged by size:
3099 642 459 422 209 133
TatI w'GTAC_w
Cuts at: 1488 1866 4479 1488
Size: 378 2613 1973
Fragments arranged by size:
2613 1973 378
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 629 661 4481 4513 629
Size: 32 3820 32 1080
Fragments arranged by size:
3820 1080 32 32
Tth111I GACn'n_nGTC
Cuts at: 2761 2761
Size: 4964
XbaI T'CTAG_A
Cuts at: 1086 1995 1086
Size: 909 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2459 2459
Size: 4964
XmaI C'CCGG_G
Cuts at: 257 1984 257
Size: 1727 3237
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BstZ17I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AleI AloI AscI AvrII BaeI
BfrBI BglI BmgBI BpmI BsaXI BsiWI BsmBI BssHII
BstEII BstXI Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI