Restriction map for
pF1KE2422
(Circular) (Six-base) MAPSORT of: pF1KE2422.seq Check: 27 from: 1 to: 3761
pF1KE2422 3761 bp
With 182 enzymes: SgfI *
October 5, 2012 11:57 ..
AccI GT'mk_AC
Cuts at: 799 799
Size: 3761
Acc65I G'GTAC_C
Cuts at: 777 777
Size: 3761
AclI AA'CG_TT
Cuts at: 3601 3601
Size: 3761
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 610 1586 2018 2765 3033 272
Size: 338 976 432 747 268 1000
Fragments arranged by size:
1000 976 747 432 338 268
AflIII A'CryG_T
Cuts at: 1113 2217 3194 1113
Size: 1104 977 1680
Fragments arranged by size:
1680 1104 977
AgeI A'CCGG_T
Cuts at: 2149 2149
Size: 3761
AlwNI CAG_nnn'CTG
Cuts at: 1066 2633 1066
Size: 1567 2194
ApaLI G'TGCA_C
Cuts at: 2531 2531
Size: 3761
ApoI r'AATT_y
Cuts at: 534 682 765 3114 3370 534
Size: 148 83 2349 256 925
Fragments arranged by size:
2349 925 256 148 83
AseI AT'TA_AT
Cuts at: 20 20
Size: 3761
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
AvaI C'yCGr_G
Cuts at: 781 781
Size: 3761
BamHI G'GATC_C
Cuts at: 786 786
Size: 3761
BanI G'GyrC_C
Cuts at: 777 1191 1439 1474 777
Size: 414 248 35 3064
Fragments arranged by size:
3064 414 248 35
BanII G_rGCy'C
Cuts at: 191 775 1805 191
Size: 584 1030 2147
Fragments arranged by size:
2147 1030 584
BbeI G_GCGC'C
Cuts at: 1195 1443 1195
Size: 248 3513
BciVI GTATCCnnnnn_n'
Cuts at: 1659 2420 3761 1659
Size: 761 1341 1659
Fragments arranged by size:
1659 1341 761
BclI T'GATC_A
Cuts at: 1281 1281
Size: 3761
BglII A'GATC_T
Cuts at: 261 1276 3313 261
Size: 1015 2037 709
Fragments arranged by size:
2037 1015 709
BlpI GC'TnA_GC
Cuts at: 873 873
Size: 3761
Bme1580I G_kGCm'C
Cuts at: 1386 1479 2535 1386
Size: 93 1056 2612
Fragments arranged by size:
2612 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 973 1054 1389 3533 973
Size: 81 335 2144 1201
Fragments arranged by size:
2144 1201 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 3761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 1816 2308 2606 937
Size: 879 492 298 2092
Fragments arranged by size:
2092 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3465 3465
Size: 3761
BsaAI yAC'GTr
Cuts at: 1114 1114
Size: 3761
BsaBI GATnn'nnATC
Cuts at: 6 1275 3312 6
Size: 1269 2037 455
Fragments arranged by size:
2037 1269 455
BsaHI Gr'CG_yC
Cuts at: 1192 1440 2142 3638 1192
Size: 248 702 1496 1315
Fragments arranged by size:
1496 1315 702 248
BsaWI w'CCGG_w
Cuts at: 950 1471 2149 2423 2570 2981 950
Size: 521 678 274 147 411 1730
Fragments arranged by size:
1730 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1186 1944 2521 3017 1186
Size: 758 577 496 1930
Fragments arranged by size:
1930 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 422
Size: 3761
BsiEI CG_ry'CG
Cuts at: 104 1303 1349 2557 104
Size: 1199 46 1208 1308
Fragments arranged by size:
1308 1208 1199 46
BsiHKAI G_wGCw'C
Cuts at: 191 436 775 1553 1743 2535 191
Size: 245 339 778 190 792 1417
Fragments arranged by size:
1417 792 778 339 245 190
BsmBI CGTCTCn'nnnn_
Cuts at: 487 487
Size: 3761
Bsp1286I G_dGCh'C
Cuts at: 191 436 775 1386 1479 1553 1743 1805 2535
Size: 245 339 611 93 74 190 62 730
Cuts at: 2535 191
Size: 1417
Fragments arranged by size:
1417 730 611 339 245 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2981 2981
Size: 3761
BspHI T'CATG_A
Cuts at: 2937 2937
Size: 3761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 811 1025 1327 1708 811
Size: 214 302 381 2864
Fragments arranged by size:
2864 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2053 2107 3591 3756 50
Size: 2003 54 1484 165 55
Fragments arranged by size:
2003 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1673 1673
Size: 3761
BsrFI r'CCGG_y
Cuts at: 1759 1940 2149 1759
Size: 181 209 3371
Fragments arranged by size:
3371 209 181
BsrGI T'GTAC_A
Cuts at: 343 343
Size: 3761
BssSI C'ACGA_G
Cuts at: 2032 2390 2032
Size: 358 3403
BstAPI GCAn_nnn'nTGC
Cuts at: 1138 1138
Size: 3761
BstBI TT'CG_AA
Cuts at: 2122 2122
Size: 3761
BstYI r'GATC_y
Cuts at: 1 261 786 1253 1276 1611 1857 2869 2955
Size: 260 525 467 23 335 246 1012 86
Cuts at: 2955 2967 3313 1
Size: 12 346 449
Fragments arranged by size:
1012 525 467 449 346 335 260 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1711 1892 2055 1711
Size: 181 163 3417
Fragments arranged by size:
3417 181 163
BtsI GCAGTG_nn'
Cuts at: 225 1004 225
Size: 779 2982
DraI TTT'AAA
Cuts at: 147 760 147
Size: 613 3148
DrdI GACnn_nn'nnGTC
Cuts at: 1467 2325 1467
Size: 858 2903
EaeI y'GGCC_r
Cuts at: 1346 1520 1911 1938 3283 1346
Size: 174 391 27 1345 1824
Fragments arranged by size:
1824 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1346 1346
Size: 3761
EarI CTCTTCn'nnn_
Cuts at: 138 344 696 1784 1994 138
Size: 206 352 1088 210 1905
Fragments arranged by size:
1905 1088 352 210 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1969 2279 2425 3571 1969
Size: 310 146 1146 2159
Fragments arranged by size:
2159 1146 310 146
EcoICRI GAG'CTC
Cuts at: 189 773 189
Size: 584 3177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 610 1586 2018 2765 3033 272
Size: 338 976 432 747 268 1000
Fragments arranged by size:
1000 976 747 432 338 268
EcoO109I rG'GnC_Cy
Cuts at: 900 900
Size: 3761
EcoRI G'AATT_C
Cuts at: 682 765 682
Size: 83 3678
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 229 1780 229
Size: 1551 2210
FspI TGC'GCA
Cuts at: 1542 1542
Size: 3761
HaeII r_GCGC'y
Cuts at: 1195 1443 2465 3444 1195
Size: 248 1022 979 1512
Fragments arranged by size:
1512 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1603 1633 1635 1665 1603
Size: 30 2 30 3699
Fragments arranged by size:
3699 30 30 2
HincII GTy'rAC
Cuts at: 285 800 285
Size: 515 3246
HpaI GTT'AAC
Cuts at: 285 285
Size: 3761
KasI G'GCGC_C
Cuts at: 1191 1439 1191
Size: 248 3513
KpnI G_GTAC'C
Cuts at: 781 781
Size: 3761
MluI A'CGCG_T
Cuts at: 3194 3194
Size: 3761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 464 2432 2616 464
Size: 1968 184 1609
Fragments arranged by size:
1968 1609 184
MscI TGG'CCA
Cuts at: 1522 1522
Size: 3761
MslI CAynn'nnrTG
Cuts at: 182 544 1750 1877 182
Size: 362 1206 127 2066
Fragments arranged by size:
2066 1206 362 127
MspA1I CmG'CkG
Cuts at: 373 872 1186 1546 2559 2804 373
Size: 499 314 360 1013 245 1330
Fragments arranged by size:
1330 1013 499 360 314 245
NaeI GCC'GGC
Cuts at: 1942 1942
Size: 3761
NarI GG'CG_CC
Cuts at: 1192 1440 1192
Size: 248 3513
NgoMIV G'CCGG_C
Cuts at: 1940 1940
Size: 3761
NspI r_CATG'y
Cuts at: 318 814 1314 2221 318
Size: 496 500 907 1858
Fragments arranged by size:
1858 907 500 496
PciI A'CATG_T
Cuts at: 2217 2217
Size: 3761
PfoI T'CCnGG_A
Cuts at: 677 677
Size: 3761
PmeI GTTT'AAAC
Cuts at: 760 760
Size: 3761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2900 2932 2900
Size: 32 3729
PstI C_TGCA'G
Cuts at: 808 1493 808
Size: 685 3076
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3761
PvuII CAG'CTG
Cuts at: 373 1186 1546 373
Size: 813 360 2588
Fragments arranged by size:
2588 813 360
RsrII CG'GwC_CG
Cuts at: 1956 1956
Size: 3761
SacI G_AGCT'C
Cuts at: 191 775 191
Size: 584 3177
SalI G'TCGA_C
Cuts at: 798 798
Size: 3761
SapI GCTCTTCn'nnn_
Cuts at: 138 1784 1994 138
Size: 1646 210 1905
Fragments arranged by size:
1905 1646 210
SbfI CC_TGCA'GG
Cuts at: 808 808
Size: 3761
ScaI AGT'ACT
Cuts at: 3278 3278
Size: 3761
SfcI C'TryA_G
Cuts at: 33 804 1489 2482 2673 33
Size: 771 685 993 191 1121
Fragments arranged by size:
1121 993 771 685 191
SfoI GGC'GCC
Cuts at: 1193 1441 1193
Size: 248 3513
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
SmaI CCC'GGG
Cuts at: 783 783
Size: 3761
SmlI C'TyrA_G
Cuts at: 916 1831 2323 2585 916
Size: 915 492 262 2092
Fragments arranged by size:
2092 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3761
SphI G_CATG'C
Cuts at: 814 1314 814
Size: 500 3261
StyI C'CwwG_G
Cuts at: 895 1249 895
Size: 354 3407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1344 1477 1936 2145 1344
Size: 133 459 209 2960
Fragments arranged by size:
2960 459 209 133
TatI w'GTAC_w
Cuts at: 343 3276 343
Size: 2933 828
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 3729
Tth111I GACn'n_nGTC
Cuts at: 1558 1558
Size: 3761
XbaI T'CTAG_A
Cuts at: 792 792
Size: 3761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1256 1256
Size: 3761
XmaI C'CCGG_G
Cuts at: 781 781
Size: 3761
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI
BsmI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI