Restriction map for
pF1KE2419
(Circular) (Six-base) MAPSORT of: pF1KE2419.seq Check: 2161 from: 1 to: 5183
pF1KE2419 5183 bp
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 705 705
Size: 5183
AccI GT'mk_AC
Cuts at: 2221 2221
Size: 5183
Acc65I G'GTAC_C
Cuts at: 1613 2199 1613
Size: 586 4597
AclI AA'CG_TT
Cuts at: 5023 5023
Size: 5183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 492 1927 3008 3440 4187 4455 316
Size: 176 1435 1081 432 747 268 1044
Fragments arranged by size:
1435 1081 1044 747 432 268 176
AflIII A'CryG_T
Cuts at: 1848 2535 3639 4616 1848
Size: 687 1104 977 2415
Fragments arranged by size:
2415 1104 977 687
AgeI A'CCGG_T
Cuts at: 3571 3571
Size: 5183
AleI CACnn'nnGTG
Cuts at: 301 301
Size: 5183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 203 1225 203
Size: 1022 4161
AlwNI CAG_nnn'CTG
Cuts at: 2488 4055 2488
Size: 1567 3616
ApaLI G'TGCA_C
Cuts at: 3953 3953
Size: 5183
ApoI r'AATT_y
Cuts at: 1173 2187 4536 4792 1173
Size: 1014 2349 256 1564
Fragments arranged by size:
2349 1564 1014 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5183
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5183
AvaI C'yCGr_G
Cuts at: 339 829 2203 339
Size: 490 1374 3319
Fragments arranged by size:
3319 1374 490
BamHI G'GATC_C
Cuts at: 2208 2208
Size: 5183
BanI G'GyrC_C
Cuts at: 234 1201 1498 1613 2199 2613 2861 2896 234
Size: 967 297 115 586 414 248 35 2521
Fragments arranged by size:
2521 967 586 414 297 248 115 35
BanII G_rGCy'C
Cuts at: 296 586 1273 1489 2197 3227 296
Size: 290 687 216 708 1030 2252
Fragments arranged by size:
2252 1030 708 687 290 216
BbeI G_GCGC'C
Cuts at: 2617 2865 2617
Size: 248 4935
BbsI GAAGACnn'nnnn_
Cuts at: 1315 1689 1788 1315
Size: 374 99 4710
Fragments arranged by size:
4710 374 99
BciVI GTATCCnnnnn_n'
Cuts at: 3081 3842 5183 3081
Size: 761 1341 3081
Fragments arranged by size:
3081 1341 761
BclI T'GATC_A
Cuts at: 1247 1706 2703 1247
Size: 459 997 3727
Fragments arranged by size:
3727 997 459
BfrBI ATG'CAT
Cuts at: 1546 1546
Size: 5183
BglI GCCn_nnn'nGGC
Cuts at: 1519 1519
Size: 5183
BglII A'GATC_T
Cuts at: 891 2698 4735 891
Size: 1807 2037 1339
Fragments arranged by size:
2037 1807 1339
BlpI GC'TnA_GC
Cuts at: 2295 2295
Size: 5183
Bme1580I G_kGCm'C
Cuts at: 1204 2808 2901 3957 1204
Size: 1604 93 1056 2430
Fragments arranged by size:
2430 1604 1056 93
BmgBI CAC'GTC
Cuts at: 1418 1418
Size: 5183
BmrI ACTGGGnnnn_n'
Cuts at: 2395 2476 2811 4955 2395
Size: 81 335 2144 2623
Fragments arranged by size:
2623 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3550 3550
Size: 5183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 570 1546 1852 570
Size: 976 306 3901
Fragments arranged by size:
3901 976 306
Bpu10I CC'TnA_GC
Cuts at: 1527 2129 1527
Size: 602 4581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1264 2359 3238 3730 4028 1264
Size: 1095 879 492 298 2419
Fragments arranged by size:
2419 1095 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 202 2089 4887 202
Size: 1887 2798 498
Fragments arranged by size:
2798 1887 498
BsaAI yAC'GTr
Cuts at: 2536 2536
Size: 5183
BsaBI GATnn'nnATC
Cuts at: 6 1441 2697 4734 6
Size: 1435 1256 2037 455
Fragments arranged by size:
2037 1435 1256 455
BsaHI Gr'CG_yC
Cuts at: 2614 2862 3564 5060 2614
Size: 248 702 1496 2737
Fragments arranged by size:
2737 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2372 2893 3571 3845 3992 4403 2372
Size: 521 678 274 147 411 3152
Fragments arranged by size:
3152 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 746 776 1060 1090 1805 1835 746
Size: 30 284 30 715 30 4094
Fragments arranged by size:
4094 715 284 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1566 2068 1566
Size: 502 4681
BseYI C'CCAG_C
Cuts at: 137 842 1482 1659 2608 3366 3943 4439 137
Size: 705 640 177 949 758 577 496 881
Fragments arranged by size:
949 881 758 705 640 577 496 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 1056 598
Size: 458 4725
BsiEI CG_ry'CG
Cuts at: 104 2725 2771 3979 104
Size: 2621 46 1208 1308
Fragments arranged by size:
2621 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 448 586 800 1280 2197 2975 3165 3957 448
Size: 138 214 480 917 778 190 792 1674
Fragments arranged by size:
1674 917 792 778 480 214 190 138
BsmI GAATG_Cn'
Cuts at: 2170 2170
Size: 5183
BsmBI CGTCTCn'nnnn_
Cuts at: 1932 1932
Size: 5183
Bsp1286I G_dGCh'C
Cuts at: 296 448 586 800 1204 1273 1280 1489 2197
Size: 152 138 214 404 69 7 209 708
Cuts at: 2197 2808 2901 2975 3165 3227 3957 296
Size: 611 93 74 190 62 730 1522
Fragments arranged by size:
1522 730 708 611 404 214 209 190
152 138 93 74 69 62 7
BspEI T'CCGG_A
Cuts at: 4403 4403
Size: 5183
BspHI T'CATG_A
Cuts at: 1250 4359 1250
Size: 3109 2074
BspMI ACCTGCnnnn'nnnn_
Cuts at: 163 705 1278 2233 2447 2749 3130 163
Size: 542 573 955 214 302 381 2216
Fragments arranged by size:
2216 955 573 542 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3475 3529 5013 5178 50
Size: 3425 54 1484 165 55
Fragments arranged by size:
3425 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 628 974 1548 3095 628
Size: 346 574 1547 2716
Fragments arranged by size:
2716 1547 574 346
BsrFI r'CCGG_y
Cuts at: 1756 1939 3181 3362 3571 1756
Size: 183 1242 181 209 3368
Fragments arranged by size:
3368 1242 209 183 181
BssSI C'ACGA_G
Cuts at: 3454 3812 3454
Size: 358 4825
BstAPI GCAn_nnn'nTGC
Cuts at: 2036 2039 2560 2036
Size: 3 521 4659
Fragments arranged by size:
4659 521 3
BstBI TT'CG_AA
Cuts at: 3544 3544
Size: 5183
BstEII G'GTnAC_C
Cuts at: 564 564
Size: 5183
BstXI CCAn_nnnn'nTGG
Cuts at: 303 400 481 663 1647 1659 303
Size: 97 81 182 984 12 3827
Fragments arranged by size:
3827 984 182 97 81 12
BstYI r'GATC_y
Cuts at: 1 891 1188 1262 2208 2675 2698 3033 3279
Size: 890 297 74 946 467 23 335 246
Cuts at: 3279 4291 4377 4389 4735 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 946 890 467 449 346 335 297
246 86 74 23 12
BtgI C'CryG_G
Cuts at: 107 272 806 107
Size: 165 534 4484
Fragments arranged by size:
4484 534 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1131 3133 3314 3477 1131
Size: 2002 181 163 2837
Fragments arranged by size:
2837 2002 181 163
BtsI GCAGTG_nn'
Cuts at: 185 2426 185
Size: 2241 2942
DraI TTT'AAA
Cuts at: 2182 2182
Size: 5183
DrdI GACnn_nn'nnGTC
Cuts at: 2889 3747 2889
Size: 858 4325
EaeI y'GGCC_r
Cuts at: 815 1361 1520 1649 1754 2768 2942 3333 3360
Size: 546 159 129 105 1014 174 391 27
Cuts at: 3360 4705 815
Size: 1345 1293
Fragments arranged by size:
1345 1293 1014 546 391 174 159 129
105 27
EagI C'GGCC_G
Cuts at: 2768 2768
Size: 5183
EarI CTCTTCn'nnn_
Cuts at: 335 630 735 3206 3416 335
Size: 295 105 2471 210 2102
Fragments arranged by size:
2471 2102 295 210 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3391 3701 3847 4993 3391
Size: 310 146 1146 3581
Fragments arranged by size:
3581 1146 310 146
EcoICRI GAG'CTC
Cuts at: 584 2195 584
Size: 1611 3572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 492 570 1546 1852 1927 3008 3440 4187
Size: 176 78 976 306 75 1081 432 747
Cuts at: 4187 4455 316
Size: 268 1044
Fragments arranged by size:
1081 1044 976 747 432 306 268 176
78 75
EcoO109I rG'GnC_Cy
Cuts at: 2139 2322 2139
Size: 183 5000
EcoRI G'AATT_C
Cuts at: 2187 2187
Size: 5183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3202 3202
Size: 5183
FspI TGC'GCA
Cuts at: 2964 2964
Size: 5183
HaeII r_GCGC'y
Cuts at: 470 2617 2865 3887 4866 470
Size: 2147 248 1022 979 787
Fragments arranged by size:
2147 1022 979 787 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 746 778 1455 1487 3025 3055 3057 3087 746
Size: 32 677 32 1538 30 2 30 2842
Fragments arranged by size:
2842 1538 677 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1349 2222 1349
Size: 873 4310
KasI G'GCGC_C
Cuts at: 2613 2861 2613
Size: 248 4935
KpnI G_GTAC'C
Cuts at: 1617 2203 1617
Size: 586 4597
MluI A'CGCG_T
Cuts at: 1848 4616 1848
Size: 2768 2415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 462 554 1072 1514 1848 3854 4038 462
Size: 92 518 442 334 2006 184 1607
Fragments arranged by size:
2006 1607 518 442 334 184 92
MscI TGG'CCA
Cuts at: 817 1363 1651 2944 817
Size: 546 288 1293 3056
Fragments arranged by size:
3056 1293 546 288
MslI CAynn'nnrTG
Cuts at: 301 661 715 1645 3172 3299 301
Size: 360 54 930 1527 127 2185
Fragments arranged by size:
2185 1527 930 360 127 54
MspA1I CmG'CkG
Cuts at: 477 939 1731 1890 2027 2294 2608 2968 3981
Size: 462 792 159 137 267 314 360 1013
Cuts at: 3981 4226 477
Size: 245 1434
Fragments arranged by size:
1434 1013 792 462 360 314 267 245
159 137
NaeI GCC'GGC
Cuts at: 1758 3364 1758
Size: 1606 3577
NarI GG'CG_CC
Cuts at: 2614 2862 2614
Size: 248 4935
NcoI C'CATG_G
Cuts at: 107 107
Size: 5183
NgoMIV G'CCGG_C
Cuts at: 1756 3362 1756
Size: 1606 3577
NsiI A_TGCA'T
Cuts at: 1548 1548
Size: 5183
NspI r_CATG'y
Cuts at: 2236 2736 3643 2236
Size: 500 907 3776
Fragments arranged by size:
3776 907 500
PasI CC'CwG_GG
Cuts at: 1913 1913
Size: 5183
PciI A'CATG_T
Cuts at: 3639 3639
Size: 5183
PfoI T'CCnGG_A
Cuts at: 1184 1184
Size: 5183
PmeI GTTT'AAAC
Cuts at: 2182 2182
Size: 5183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4322 4354 4322
Size: 32 5151
PpuMI rG'GwC_Cy
Cuts at: 2139 2139
Size: 5183
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1672 1704 1672
Size: 32 5151
PstI C_TGCA'G
Cuts at: 1835 2032 2230 2915 1835
Size: 197 198 685 4103
Fragments arranged by size:
4103 685 198 197
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5183
PvuII CAG'CTG
Cuts at: 477 939 2608 2968 477
Size: 462 1669 360 2692
Fragments arranged by size:
2692 1669 462 360
RsrII CG'GwC_CG
Cuts at: 3378 3378
Size: 5183
SacI G_AGCT'C
Cuts at: 586 2197 586
Size: 1611 3572
SalI G'TCGA_C
Cuts at: 2220 2220
Size: 5183
SapI GCTCTTCn'nnn_
Cuts at: 735 3206 3416 735
Size: 2471 210 2502
Fragments arranged by size:
2502 2471 210
SbfI CC_TGCA'GG
Cuts at: 2230 2230
Size: 5183
ScaI AGT'ACT
Cuts at: 4700 4700
Size: 5183
SfcI C'TryA_G
Cuts at: 33 1831 2028 2226 2911 3904 4095 33
Size: 1798 197 198 685 993 191 1121
Fragments arranged by size:
1798 1121 993 685 198 197 191
SfoI GGC'GCC
Cuts at: 2615 2863 2615
Size: 248 4935
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5183
SmaI CCC'GGG
Cuts at: 2205 2205
Size: 5183
SmlI C'TyrA_G
Cuts at: 829 1279 2338 3253 3745 4007 829
Size: 450 1059 915 492 262 2005
Fragments arranged by size:
2005 1059 915 492 450 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5183
SphI G_CATG'C
Cuts at: 2236 2736 2236
Size: 500 4683
StyI C'CwwG_G
Cuts at: 107 393 1652 2174 2317 2671 107
Size: 286 1259 522 143 354 2619
Fragments arranged by size:
2619 1259 522 354 286 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 835 2176 2766 2899 3358 3567 835
Size: 1341 590 133 459 209 2451
Fragments arranged by size:
2451 1341 590 459 209 133
TatI w'GTAC_w
Cuts at: 4698 4698
Size: 5183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1433 1465 4700 4732 1433
Size: 32 3235 32 1884
Fragments arranged by size:
3235 1884 32 32
Tth111I GACn'n_nGTC
Cuts at: 2980 2980
Size: 5183
XbaI T'CTAG_A
Cuts at: 2214 2214
Size: 5183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 537 871 1515 2678 537
Size: 334 644 1163 3042
Fragments arranged by size:
3042 1163 644 334
XhoI C'TCGA_G
Cuts at: 829 829
Size: 5183
XmaI C'CCGG_G
Cuts at: 2203 2203
Size: 5183
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PfoI PmeI PpiI PpuMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BmtI BsiWI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PflMI PmlI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI ZraI