Restriction map for
pF1KE2394
(Circular) (Six-base) MAPSORT of: pF1KE2394.seq Check: 5043 from: 1 to: 5576
pF1KE2394 5576 bp
With 182 enzymes: SgfI *
January 26, 2012 13:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 516 516
Size: 5576
AatII G_ACGT'C
Cuts at: 869 1600 869
Size: 731 4845
AccI GT'mk_AC
Cuts at: 1358 2614 1358
Size: 1256 4320
Acc65I G'GTAC_C
Cuts at: 2592 2592
Size: 5576
AclI AA'CG_TT
Cuts at: 5416 5416
Size: 5576
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 818 2012 2414 3401 3833 4580 4848 818
Size: 1194 402 987 432 747 268 1546
Fragments arranged by size:
1546 1194 987 747 432 402 268
AflII C'TTAA_G
Cuts at: 2244 2244
Size: 5576
AflIII A'CryG_T
Cuts at: 2037 2928 4032 5009 2037
Size: 891 1104 977 2604
Fragments arranged by size:
2604 1104 977 891
AgeI A'CCGG_T
Cuts at: 3964 3964
Size: 5576
AleI CACnn'nnGTG
Cuts at: 2015 2015
Size: 5576
AlwNI CAG_nnn'CTG
Cuts at: 153 192 1402 2881 4448 153
Size: 39 1210 1479 1567 1281
Fragments arranged by size:
1567 1479 1281 1210 39
ApaI G_GGCC'C
Cuts at: 966 966
Size: 5576
ApaLI G'TGCA_C
Cuts at: 4346 4346
Size: 5576
ApoI r'AATT_y
Cuts at: 1489 1668 2580 4929 5185 1489
Size: 179 912 2349 256 1880
Fragments arranged by size:
2349 1880 912 256 179
AseI AT'TA_AT
Cuts at: 20 20
Size: 5576
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5576
AvaI C'yCGr_G
Cuts at: 755 2596 755
Size: 1841 3735
BamHI G'GATC_C
Cuts at: 2601 2601
Size: 5576
BanI G'GyrC_C
Cuts at: 136 2592 3006 3254 3289 136
Size: 2456 414 248 35 2423
Fragments arranged by size:
2456 2423 414 248 35
BanII G_rGCy'C
Cuts at: 485 966 1052 2477 2590 3620 485
Size: 481 86 1425 113 1030 2441
Fragments arranged by size:
2441 1425 1030 481 113 86
BbeI G_GCGC'C
Cuts at: 140 3010 3258 140
Size: 2870 248 2458
Fragments arranged by size:
2870 2458 248
BbsI GAAGACnn'nnnn_
Cuts at: 1934 2216 1934
Size: 282 5294
BciVI GTATCCnnnnn_n'
Cuts at: 681 775 3474 4235 5576 681
Size: 94 2699 761 1341 681
Fragments arranged by size:
2699 1341 761 681 94
BclI T'GATC_A
Cuts at: 599 2457 3096 599
Size: 1858 639 3079
Fragments arranged by size:
3079 1858 639
BglII A'GATC_T
Cuts at: 3091 5128 3091
Size: 2037 3539
BlpI GC'TnA_GC
Cuts at: 746 877 2688 746
Size: 131 1811 3634
Fragments arranged by size:
3634 1811 131
Bme1580I G_kGCm'C
Cuts at: 966 3201 3294 4350 966
Size: 2235 93 1056 2192
Fragments arranged by size:
2235 2192 1056 93
BmgBI CAC'GTC
Cuts at: 1506 1506
Size: 5576
BmrI ACTGGGnnnn_n'
Cuts at: 538 2788 2869 3204 5348 538
Size: 2250 81 335 2144 766
Fragments arranged by size:
2250 2144 766 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 504 2406 3943 504
Size: 1902 1537 2137
Fragments arranged by size:
2137 1902 1537
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 1466 1949 175
Size: 1291 483 3802
Fragments arranged by size:
3802 1291 483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 1309 1990 2752 3631 4123 4421 800
Size: 509 681 762 879 492 298 1955
Fragments arranged by size:
1955 879 762 681 509 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 730 5280 730
Size: 4550 1026
BsaAI yAC'GTr
Cuts at: 895 1332 2929 895
Size: 437 1597 3542
Fragments arranged by size:
3542 1597 437
BsaBI GATnn'nnATC
Cuts at: 6 1139 1647 2099 3090 5127 6
Size: 1133 508 452 991 2037 455
Fragments arranged by size:
2037 1133 991 508 455 452
BsaHI Gr'CG_yC
Cuts at: 137 866 1597 3007 3255 3957 5453 137
Size: 729 731 1410 248 702 1496 260
Fragments arranged by size:
1496 1410 731 729 702 260 248
BsaWI w'CCGG_w
Cuts at: 2054 2765 3286 3964 4238 4385 4796 2054
Size: 711 521 678 274 147 411 2834
Fragments arranged by size:
2834 711 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1102 1132 1102
Size: 30 5546
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 364 687 1351 2355 226
Size: 138 323 664 1004 3447
Fragments arranged by size:
3447 1004 664 323 138
BseYI C'CCAG_C
Cuts at: 2250 3001 3759 4336 4832 2250
Size: 751 758 577 496 2994
Fragments arranged by size:
2994 758 751 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 917 917
Size: 5576
BsiEI CG_ry'CG
Cuts at: 104 3118 3164 4372 104
Size: 3014 46 1208 1308
Fragments arranged by size:
3014 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 485 894 1052 2477 2590 3368 3558 4350 485
Size: 409 158 1425 113 778 190 792 1711
Fragments arranged by size:
1711 1425 792 778 409 190 158 113
BsmI GAATG_Cn'
Cuts at: 445 947 1773 445
Size: 502 826 4248
Fragments arranged by size:
4248 826 502
BsmBI CGTCTCn'nnnn_
Cuts at: 873 1990 873
Size: 1117 4459
Bsp1286I G_dGCh'C
Cuts at: 485 894 966 1052 2477 2590 3201 3294 3368
Size: 409 72 86 1425 113 611 93 74
Cuts at: 3368 3558 3620 4350 485
Size: 190 62 730 1711
Fragments arranged by size:
1711 1425 730 611 409 190 113 93
86 74 72 62
BspEI T'CCGG_A
Cuts at: 4796 4796
Size: 5576
BspHI T'CATG_A
Cuts at: 1425 1527 4752 1425
Size: 102 3225 2249
Fragments arranged by size:
3225 2249 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 464 516 2626 2840 3142 3523 464
Size: 52 2110 214 302 381 2517
Fragments arranged by size:
2517 2110 381 302 214 52
BsrBI CCG'CTC
Cuts at: 50 566 3868 3922 5406 5571 50
Size: 516 3302 54 1484 165 55
Fragments arranged by size:
3302 1484 516 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1194 1748 3488 1194
Size: 554 1740 3282
Fragments arranged by size:
3282 1740 554
BsrFI r'CCGG_y
Cuts at: 139 3574 3755 3964 139
Size: 3435 181 209 1751
Fragments arranged by size:
3435 1751 209 181
BssSI C'ACGA_G
Cuts at: 3847 4205 3847
Size: 358 5218
BstAPI GCAn_nnn'nTGC
Cuts at: 531 2953 531
Size: 2422 3154
BstBI TT'CG_AA
Cuts at: 3937 3937
Size: 5576
BstEII G'GTnAC_C
Cuts at: 1294 1294
Size: 5576
BstXI CCAn_nnnn'nTGG
Cuts at: 1921 1921
Size: 5576
BstYI r'GATC_y
Cuts at: 1 416 2120 2529 2601 3068 3091 3426 3672
Size: 415 1704 409 72 467 23 335 246
Cuts at: 3672 4684 4770 4782 5128 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1704 1012 467 449 415 409 346 335
246 86 72 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5576
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 242 3526 3707 3870 242
Size: 3284 181 163 1948
Fragments arranged by size:
3284 1948 181 163
BtsI GCAGTG_nn'
Cuts at: 2010 2819 2010
Size: 809 4767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1138 1173 1138
Size: 35 5541
DraI TTT'AAA
Cuts at: 2575 2575
Size: 5576
DrdI GACnn_nn'nnGTC
Cuts at: 3282 4140 3282
Size: 858 4718
EaeI y'GGCC_r
Cuts at: 173 3161 3335 3726 3753 5098 173
Size: 2988 174 391 27 1345 651
Fragments arranged by size:
2988 1345 651 391 174 27
EagI C'GGCC_G
Cuts at: 3161 3161
Size: 5576
EarI CTCTTCn'nnn_
Cuts at: 1268 1708 2111 2312 3599 3809 1268
Size: 440 403 201 1287 210 3035
Fragments arranged by size:
3035 1287 440 403 210 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1900 3784 4094 4240 5386 1900
Size: 1884 310 146 1146 2090
Fragments arranged by size:
2090 1884 1146 310 146
EcoICRI GAG'CTC
Cuts at: 483 1050 2475 2588 483
Size: 567 1425 113 3471
Fragments arranged by size:
3471 1425 567 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 818 1466 1949 2012 2414 3401 3833 4580
Size: 643 648 483 63 402 987 432 747
Cuts at: 4580 4848 175
Size: 268 903
Fragments arranged by size:
987 903 747 648 643 483 432 402
268 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2244 2302 2244
Size: 58 5518
EcoO109I rG'GnC_Cy
Cuts at: 963 978 1256 2715 963
Size: 15 278 1459 3824
Fragments arranged by size:
3824 1459 278 15
EcoRI G'AATT_C
Cuts at: 2580 2580
Size: 5576
EcoRV GAT'ATC
Cuts at: 1821 1831 1821
Size: 10 5566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 766 852 2419 3595 766
Size: 86 1567 1176 2747
Fragments arranged by size:
2747 1567 1176 86
FspI TGC'GCA
Cuts at: 3357 3357
Size: 5576
HaeII r_GCGC'y
Cuts at: 140 3010 3258 4280 5259 140
Size: 2870 248 1022 979 457
Fragments arranged by size:
2870 1022 979 457 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 749 781 1405 1437 1685 1717 1820 1852 2311
Size: 32 624 32 248 32 103 32 459
Cuts at: 2311 2343 3418 3448 3450 3480 749
Size: 32 1075 30 2 30 2845
Fragments arranged by size:
2845 1075 624 459 248 103 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2355 2547 2615 2355
Size: 192 68 5316
Fragments arranged by size:
5316 192 68
HindIII A'AGCT_T
Cuts at: 874 2048 874
Size: 1174 4402
HpaI GTT'AAC
Cuts at: 2355 2547 2355
Size: 192 5384
KasI G'GCGC_C
Cuts at: 136 3006 3254 136
Size: 2870 248 2458
Fragments arranged by size:
2870 2458 248
KpnI G_GTAC'C
Cuts at: 2596 2596
Size: 5576
MfeI C'AATT_G
Cuts at: 1793 1793
Size: 5576
MluI A'CGCG_T
Cuts at: 5009 5009
Size: 5576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2052 4247 4431 2052
Size: 2195 184 3197
Fragments arranged by size:
3197 2195 184
MscI TGG'CCA
Cuts at: 3337 3337
Size: 5576
MslI CAynn'nnrTG
Cuts at: 1187 2015 2080 3565 3692 1187
Size: 828 65 1485 127 3071
Fragments arranged by size:
3071 1485 828 127 65
MspA1I CmG'CkG
Cuts at: 127 1072 1402 2687 3001 3361 4374 4619 127
Size: 945 330 1285 314 360 1013 245 1084
Fragments arranged by size:
1285 1084 1013 945 360 330 314 245
NaeI GCC'GGC
Cuts at: 141 3757 141
Size: 3616 1960
NarI GG'CG_CC
Cuts at: 137 3007 3255 137
Size: 2870 248 2458
Fragments arranged by size:
2870 2458 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5576
NgoMIV G'CCGG_C
Cuts at: 139 3755 139
Size: 3616 1960
NspI r_CATG'y
Cuts at: 2041 2629 3129 4036 2041
Size: 588 500 907 3581
Fragments arranged by size:
3581 907 588 500
PciI A'CATG_T
Cuts at: 2037 4032 2037
Size: 1995 3581
PflMI CCAn_nnn'nTGG
Cuts at: 153 1036 1627 153
Size: 883 591 4102
Fragments arranged by size:
4102 883 591
PfoI T'CCnGG_A
Cuts at: 197 197
Size: 5576
PmeI GTTT'AAAC
Cuts at: 2575 2575
Size: 5576
PmlI CAC'GTG
Cuts at: 895 1332 895
Size: 437 5139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1908 1940 4715 4747 1908
Size: 32 2775 32 2737
Fragments arranged by size:
2775 2737 32 32
PpuMI rG'GwC_Cy
Cuts at: 978 1256 978
Size: 278 5298
PsiI TTA'TAA
Cuts at: 2407 2407
Size: 5576
PspOMI G'GGCC_C
Cuts at: 962 962
Size: 5576
PspXI vC'TCGA_Gb
Cuts at: 755 755
Size: 5576
PstI C_TGCA'G
Cuts at: 2623 3308 2623
Size: 685 4891
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5576
PvuII CAG'CTG
Cuts at: 1072 1402 3001 3361 1072
Size: 330 1599 360 3287
Fragments arranged by size:
3287 1599 360 330
RsrII CG'GwC_CG
Cuts at: 3771 3771
Size: 5576
SacI G_AGCT'C
Cuts at: 485 1052 2477 2590 485
Size: 567 1425 113 3471
Fragments arranged by size:
3471 1425 567 113
SalI G'TCGA_C
Cuts at: 2613 2613
Size: 5576
SapI GCTCTTCn'nnn_
Cuts at: 3599 3809 3599
Size: 210 5366
SbfI CC_TGCA'GG
Cuts at: 2623 2623
Size: 5576
ScaI AGT'ACT
Cuts at: 2266 5093 2266
Size: 2827 2749
SfcI C'TryA_G
Cuts at: 33 357 2619 3304 4297 4488 33
Size: 324 2262 685 993 191 1121
Fragments arranged by size:
2262 1121 993 685 324 191
SfoI GGC'GCC
Cuts at: 138 3008 3256 138
Size: 2870 248 2458
Fragments arranged by size:
2870 2458 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5576
SmaI CCC'GGG
Cuts at: 2598 2598
Size: 5576
SmlI C'TyrA_G
Cuts at: 755 815 1324 1969 2244 2731 3646 4138 4400
Size: 60 509 645 275 487 915 492 262
Cuts at: 4400 755
Size: 1931
Fragments arranged by size:
1931 915 645 509 492 487 275 262
60
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5576
SphI G_CATG'C
Cuts at: 2629 3129 2629
Size: 500 5076
StyI C'CwwG_G
Cuts at: 107 428 966 1030 2710 3064 107
Size: 321 538 64 1680 354 2619
Fragments arranged by size:
2619 1680 538 354 321 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 557 566 2494 3159 3292 3751 3960 557
Size: 9 1928 665 133 459 209 2173
Fragments arranged by size:
2173 1928 665 459 209 133 9
TatI w'GTAC_w
Cuts at: 1303 2264 5091 1303
Size: 961 2827 1788
Fragments arranged by size:
2827 1788 961
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5093 5125 5093
Size: 32 5544
Tth111I GACn'n_nGTC
Cuts at: 1938 3373 1938
Size: 1435 4141
XbaI T'CTAG_A
Cuts at: 2103 2607 2103
Size: 504 5072
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3071 3071
Size: 5576
XhoI C'TCGA_G
Cuts at: 755 755
Size: 5576
XmaI C'CCGG_G
Cuts at: 2596 2596
Size: 5576
XmnI GAAnn'nnTTC
Cuts at: 193 266 394 947 193
Size: 73 128 553 4822
Fragments arranged by size:
4822 553 128 73
ZraI GAC'GTC
Cuts at: 867 1598 867
Size: 731 4845
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmtI Bpu10I BsiWI BsrGI BssHII
BstZ17I Bsu36I ClaI DraIII FseI FspAI NdeI NheI
NotI NruI NsiI PacI PasI PshAI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI