Restriction map for
pF1KE2390
(Circular) (Six-base) MAPSORT of: pF1KE2390.seq Check: 3835 from: 1 to: 4616
pF1KE2390 4616 bp
With 182 enzymes: SgfI *
January 17, 2012 13:59 ..
AccI GT'mk_AC
Cuts at: 192 1654 192
Size: 1462 3154
Acc65I G'GTAC_C
Cuts at: 1632 1632
Size: 4616
AclI AA'CG_TT
Cuts at: 4456 4456
Size: 4616
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 2441 2873 3620 3888 1495
Size: 946 432 747 268 2223
Fragments arranged by size:
2223 946 747 432 268
AflIII A'CryG_T
Cuts at: 1968 3072 4049 1968
Size: 1104 977 2535
Fragments arranged by size:
2535 1104 977
AgeI A'CCGG_T
Cuts at: 3004 3004
Size: 4616
AleI CACnn'nnGTG
Cuts at: 334 607 334
Size: 273 4343
AlwNI CAG_nnn'CTG
Cuts at: 557 1921 3488 557
Size: 1364 1567 1685
Fragments arranged by size:
1685 1567 1364
ApaLI G'TGCA_C
Cuts at: 3386 3386
Size: 4616
ApoI r'AATT_y
Cuts at: 471 1620 3969 4225 471
Size: 1149 2349 256 862
Fragments arranged by size:
2349 1149 862 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4616
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
AvaI C'yCGr_G
Cuts at: 537 1201 1636 537
Size: 664 435 3517
Fragments arranged by size:
3517 664 435
AvrII C'CTAG_G
Cuts at: 778 778
Size: 4616
BamHI G'GATC_C
Cuts at: 1641 1641
Size: 4616
BanI G'GyrC_C
Cuts at: 150 1632 2046 2294 2329 150
Size: 1482 414 248 35 2437
Fragments arranged by size:
2437 1482 414 248 35
BanII G_rGCy'C
Cuts at: 123 944 1226 1630 2660 123
Size: 821 282 404 1030 2079
Fragments arranged by size:
2079 1030 821 404 282
BbeI G_GCGC'C
Cuts at: 154 2050 2298 154
Size: 1896 248 2472
Fragments arranged by size:
2472 1896 248
BbsI GAAGACnn'nnnn_
Cuts at: 897 897
Size: 4616
BciVI GTATCCnnnnn_n'
Cuts at: 2514 3275 4616 2514
Size: 761 1341 2514
Fragments arranged by size:
2514 1341 761
BclI T'GATC_A
Cuts at: 2136 2136
Size: 4616
BfrBI ATG'CAT
Cuts at: 875 875
Size: 4616
BglI GCCn_nnn'nGGC
Cuts at: 753 753
Size: 4616
BglII A'GATC_T
Cuts at: 1151 2131 4168 1151
Size: 980 2037 1599
Fragments arranged by size:
2037 1599 980
BlpI GC'TnA_GC
Cuts at: 370 1728 370
Size: 1358 3258
Bme1580I G_kGCm'C
Cuts at: 2241 2334 3390 2241
Size: 93 1056 3467
Fragments arranged by size:
3467 1056 93
BmgBI CAC'GTC
Cuts at: 444 480 444
Size: 36 4580
BmrI ACTGGGnnnn_n'
Cuts at: 1828 1909 2244 4388 1828
Size: 81 335 2144 2056
Fragments arranged by size:
2144 2056 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1434 2983 1434
Size: 1549 3067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 752 1226 1580 370
Size: 382 474 354 3406
Fragments arranged by size:
3406 474 382 354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1792 2671 3163 3461 1792
Size: 879 492 298 2947
Fragments arranged by size:
2947 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 248 283 1000 1202 4320 248
Size: 35 717 202 3118 544
Fragments arranged by size:
3118 717 544 202 35
BsaAI yAC'GTr
Cuts at: 1580 1969 1580
Size: 389 4227
BsaBI GATnn'nnATC
Cuts at: 6 2130 4167 6
Size: 2124 2037 455
Fragments arranged by size:
2124 2037 455
BsaHI Gr'CG_yC
Cuts at: 151 167 1574 2047 2295 2997 4493 151
Size: 16 1407 473 248 702 1496 274
Fragments arranged by size:
1496 1407 702 473 274 248 16
BsaWI w'CCGG_w
Cuts at: 1283 1398 1805 2326 3004 3278 3425 3836 1283
Size: 115 407 521 678 274 147 411 2063
Fragments arranged by size:
2063 678 521 411 407 274 147 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 245 275 245
Size: 30 4586
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1410 1410
Size: 4616
BseYI C'CCAG_C
Cuts at: 135 144 374 1253 2041 2799 3376 3872 135
Size: 9 230 879 788 758 577 496 879
Fragments arranged by size:
879 879 788 758 577 496 230 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1087
Size: 4616
BsiEI CG_ry'CG
Cuts at: 104 2158 2204 3412 104
Size: 2054 46 1208 1308
Fragments arranged by size:
2054 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 331 392 1630 2408 2598 3390 331
Size: 61 1238 778 190 792 1557
Fragments arranged by size:
1557 1238 792 778 190 61
BsmBI CGTCTCn'nnnn_
Cuts at: 1422 1422
Size: 4616
Bsp1286I G_dGCh'C
Cuts at: 123 331 392 944 1226 1630 2241 2334 2408
Size: 208 61 552 282 404 611 93 74
Cuts at: 2408 2598 2660 3390 123
Size: 190 62 730 1349
Fragments arranged by size:
1349 730 611 552 404 282 208 190
93 74 62 61
BspEI T'CCGG_A
Cuts at: 1283 1398 3836 1283
Size: 115 2438 2063
Fragments arranged by size:
2438 2063 115
BspHI T'CATG_A
Cuts at: 699 3792 699
Size: 3093 1523
BspMI ACCTGCnnnn'nnnn_
Cuts at: 920 1109 1187 1666 1880 2182 2563 920
Size: 189 78 479 214 302 381 2973
Fragments arranged by size:
2973 479 381 302 214 189 78
BsrBI CCG'CTC
Cuts at: 50 156 1112 2908 2962 4446 4611 50
Size: 106 956 1796 54 1484 165 55
Fragments arranged by size:
1796 1484 956 165 106 55 54
BsrDI GCAATG_nn'
Cuts at: 863 2528 863
Size: 1665 2951
BsrFI r'CCGG_y
Cuts at: 2614 2795 3004 2614
Size: 181 209 4226
Fragments arranged by size:
4226 209 181
BssSI C'ACGA_G
Cuts at: 2887 3245 2887
Size: 358 4258
BstAPI GCAn_nnn'nTGC
Cuts at: 557 1993 557
Size: 1436 3180
BstBI TT'CG_AA
Cuts at: 2977 2977
Size: 4616
BstEII G'GTnAC_C
Cuts at: 570 570
Size: 4616
BstXI CCAn_nnnn'nTGG
Cuts at: 1506 1506
Size: 4616
BstYI r'GATC_y
Cuts at: 1 1151 1641 2108 2131 2466 2712 3724 3810
Size: 1150 490 467 23 335 246 1012 86
Cuts at: 3810 3822 4168 1
Size: 12 346 449
Fragments arranged by size:
1150 1012 490 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 171 1052 1499 171
Size: 881 447 3288
Fragments arranged by size:
3288 881 447
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1419 2566 2747 2910 1419
Size: 1147 181 163 3125
Fragments arranged by size:
3125 1147 181 163
BtsI GCAGTG_nn'
Cuts at: 1859 1859
Size: 4616
ClaI AT'CG_AT
Cuts at: 401 1367 401
Size: 966 3650
DraI TTT'AAA
Cuts at: 1615 1615
Size: 4616
DrdI GACnn_nn'nnGTC
Cuts at: 496 2322 3180 496
Size: 1826 858 1932
Fragments arranged by size:
1932 1826 858
EaeI y'GGCC_r
Cuts at: 235 723 893 1496 2201 2375 2766 2793 4138
Size: 488 170 603 705 174 391 27 1345
Cuts at: 4138 235
Size: 713
Fragments arranged by size:
1345 713 705 603 488 391 174 170
27
EagI C'GGCC_G
Cuts at: 2201 2201
Size: 4616
EarI CTCTTCn'nnn_
Cuts at: 314 850 893 1312 1522 2639 2849 314
Size: 536 43 419 210 1117 210 2081
Fragments arranged by size:
2081 1117 536 419 210 210 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 199 436 820 2824 3134 3280 4426 199
Size: 237 384 2004 310 146 1146 389
Fragments arranged by size:
2004 1146 389 384 310 237 146
EcoICRI GAG'CTC
Cuts at: 1628 1628
Size: 4616
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 752 1226 1495 1580 2441 2873 3620 3888
Size: 382 474 269 85 861 432 747 268
Cuts at: 3888 370
Size: 1098
Fragments arranged by size:
1098 861 747 474 432 382 269 268
85
EcoNI CCTnn'n_nnAGG
Cuts at: 782 782
Size: 4616
EcoO109I rG'GnC_Cy
Cuts at: 378 664 1553 1755 378
Size: 286 889 202 3239
Fragments arranged by size:
3239 889 286 202
EcoRI G'AATT_C
Cuts at: 1620 1620
Size: 4616
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 357 861 2635 357
Size: 504 1774 2338
Fragments arranged by size:
2338 1774 504
FspI TGC'GCA
Cuts at: 2397 2397
Size: 4616
HaeII r_GCGC'y
Cuts at: 145 154 804 2050 2298 3320 4299 145
Size: 9 650 1246 248 1022 979 462
Fragments arranged by size:
1246 1022 979 650 462 248 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 245 277 2458 2488 2490 2520 245
Size: 32 2181 30 2 30 2341
Fragments arranged by size:
2341 2181 32 30 30 2
HincII GTy'rAC
Cuts at: 193 858 1655 193
Size: 665 797 3154
Fragments arranged by size:
3154 797 665
KasI G'GCGC_C
Cuts at: 150 2046 2294 150
Size: 1896 248 2472
Fragments arranged by size:
2472 1896 248
KpnI G_GTAC'C
Cuts at: 1636 1636
Size: 4616
MluI A'CGCG_T
Cuts at: 4049 4049
Size: 4616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 536 3287 3471 536
Size: 2751 184 1681
Fragments arranged by size:
2751 1681 184
MscI TGG'CCA
Cuts at: 725 895 1498 2377 725
Size: 170 603 879 2964
Fragments arranged by size:
2964 879 603 170
MslI CAynn'nnrTG
Cuts at: 334 607 698 1504 2605 2732 334
Size: 273 91 806 1101 127 2218
Fragments arranged by size:
2218 1101 806 273 127 91
MspA1I CmG'CkG
Cuts at: 173 557 1173 1446 1727 2041 2401 3414 3659
Size: 384 616 273 281 314 360 1013 245
Cuts at: 3659 173
Size: 1130
Fragments arranged by size:
1130 1013 616 384 360 314 281 273
245
NaeI GCC'GGC
Cuts at: 2797 2797
Size: 4616
NarI GG'CG_CC
Cuts at: 151 2047 2295 151
Size: 1896 248 2472
Fragments arranged by size:
2472 1896 248
NcoI C'CATG_G
Cuts at: 1052 1499 1052
Size: 447 4169
NgoMIV G'CCGG_C
Cuts at: 2795 2795
Size: 4616
NsiI A_TGCA'T
Cuts at: 877 877
Size: 4616
NspI r_CATG'y
Cuts at: 1669 2169 3076 1669
Size: 500 907 3209
Fragments arranged by size:
3209 907 500
PasI CC'CwG_GG
Cuts at: 115 115
Size: 4616
PciI A'CATG_T
Cuts at: 3072 3072
Size: 4616
PflMI CCAn_nnn'nTGG
Cuts at: 1249 1249
Size: 4616
PfoI T'CCnGG_A
Cuts at: 197 1242 1568 197
Size: 1045 326 3245
Fragments arranged by size:
3245 1045 326
PmeI GTTT'AAAC
Cuts at: 1615 1615
Size: 4616
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 4584
PpuMI rG'GwC_Cy
Cuts at: 378 664 1553 378
Size: 286 889 3441
Fragments arranged by size:
3441 889 286
PshAI GACnn'nnGTC
Cuts at: 1558 1558
Size: 4616
PstI C_TGCA'G
Cuts at: 562 653 1451 1663 2348 562
Size: 91 798 212 685 2830
Fragments arranged by size:
2830 798 685 212 91
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4616
PvuII CAG'CTG
Cuts at: 557 1446 2041 2401 557
Size: 889 595 360 2772
Fragments arranged by size:
2772 889 595 360
RsrII CG'GwC_CG
Cuts at: 2811 2811
Size: 4616
SacI G_AGCT'C
Cuts at: 1630 1630
Size: 4616
SacII CC_GC'GG
Cuts at: 174 174
Size: 4616
SalI G'TCGA_C
Cuts at: 191 1653 191
Size: 1462 3154
SanDI GG'GwC_CC
Cuts at: 378 1553 378
Size: 1175 3441
SapI GCTCTTCn'nnn_
Cuts at: 2639 2849 2639
Size: 210 4406
SbfI CC_TGCA'GG
Cuts at: 1663 1663
Size: 4616
ScaI AGT'ACT
Cuts at: 4133 4133
Size: 4616
SexAI A'CCwGG_T
Cuts at: 851 851
Size: 4616
SfcI C'TryA_G
Cuts at: 33 363 558 621 649 1447 1659 2344 3337
Size: 330 195 63 28 798 212 685 993
Cuts at: 3337 3528 33
Size: 191 1121
Fragments arranged by size:
1121 993 798 685 330 212 195 191
63 28
SfoI GGC'GCC
Cuts at: 152 2048 2296 152
Size: 1896 248 2472
Fragments arranged by size:
2472 1896 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
SmaI CCC'GGG
Cuts at: 539 1638 539
Size: 1099 3517
SmlI C'TyrA_G
Cuts at: 1771 2686 3178 3440 1771
Size: 915 492 262 2947
Fragments arranged by size:
2947 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4616
SphI G_CATG'C
Cuts at: 1669 2169 1669
Size: 500 4116
SspI AAT'ATT
Cuts at: 1276 1276
Size: 4616
StuI AGG'CCT
Cuts at: 659 659
Size: 4616
StyI C'CwwG_G
Cuts at: 778 1052 1499 1750 2104 778
Size: 274 447 251 354 3290
Fragments arranged by size:
3290 447 354 274 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1138 2199 2332 2791 3000 1138
Size: 1061 133 459 209 2754
Fragments arranged by size:
2754 1061 459 209 133
TatI w'GTAC_w
Cuts at: 338 4131 338
Size: 3793 823
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 415 447 4133 4165 415
Size: 32 3686 32 866
Fragments arranged by size:
3686 866 32 32
Tth111I GACn'n_nGTC
Cuts at: 259 2413 259
Size: 2154 2462
XbaI T'CTAG_A
Cuts at: 1647 1647
Size: 4616
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1250 2111 1250
Size: 861 3755
XmaI C'CCGG_G
Cuts at: 537 1636 537
Size: 1099 3517
XmnI GAAnn'nnTTC
Cuts at: 208 208
Size: 4616
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BmtI Bpu10I BsiWI BsmI
BsrGI BssHII BstZ17I Bsu36I CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PmlI PsiI PspOMI PspXI PsrI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI