Restriction map for
pF1KE2387
(Circular) (Six-base) MAPSORT of: pF1KE2387.seq Check: 6625 from: 1 to: 8381
pF1KE2387 8381 bp
With 182 enzymes: SgfI *
December 8, 2011 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1257 2247 5075 1257
Size: 990 2828 4563
Fragments arranged by size:
4563 2828 990
AatII G_ACGT'C
Cuts at: 4712 4712
Size: 8381
AccI GT'mk_AC
Cuts at: 5419 5419
Size: 8381
Acc65I G'GTAC_C
Cuts at: 1169 5397 1169
Size: 4228 4153
AclI AA'CG_TT
Cuts at: 8221 8221
Size: 8381
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 457 1600 2428 2669 3254 3835 6206 6638
Size: 352 1143 828 241 585 581 2371 432
Cuts at: 6638 7385 7653 105
Size: 747 268 833
Fragments arranged by size:
2371 1143 833 828 747 585 581 432
352 268 241
AfeI AGC'GCT
Cuts at: 769 1422 4394 769
Size: 653 2972 4756
Fragments arranged by size:
4756 2972 653
AflIII A'CryG_T
Cuts at: 5156 5733 6837 7814 5156
Size: 577 1104 977 5723
Fragments arranged by size:
5723 1104 977 577
AgeI A'CCGG_T
Cuts at: 1166 6769 1166
Size: 5603 2778
AhdI GACnn_n'nnGTC
Cuts at: 5247 5247
Size: 8381
AleI CACnn'nnGTG
Cuts at: 2608 2608
Size: 8381
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 233 1748 3059 233
Size: 1515 1311 5555
Fragments arranged by size:
5555 1515 1311
AlwNI CAG_nnn'CTG
Cuts at: 708 741 948 1110 1248 1353 1477 1629 1917
Size: 33 207 162 138 105 124 152 288
Cuts at: 1917 2032 2070 2676 2685 2964 3006 3324 3942
Size: 115 38 606 9 279 42 318 618
Cuts at: 3942 4386 4533 5349 5686 7253 708
Size: 444 147 816 337 1567 1836
Fragments arranged by size:
1836 1567 816 618 606 444 337 318
288 279 207 162 152 147 138 124
115 105 42 38 33 9
ApaI G_GGCC'C
Cuts at: 647 3123 647
Size: 2476 5905
ApaLI G'TGCA_C
Cuts at: 1000 2218 7151 1000
Size: 1218 4933 2230
Fragments arranged by size:
4933 2230 1218
ApoI r'AATT_y
Cuts at: 876 4750 5385 7734 7990 876
Size: 3874 635 2349 256 1267
Fragments arranged by size:
3874 2349 1267 635 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8381
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8381
AvaI C'yCGr_G
Cuts at: 964 2154 3185 4546 4999 5401 964
Size: 1190 1031 1361 453 402 3944
Fragments arranged by size:
3944 1361 1190 1031 453 402
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4357 4390 4357
Size: 33 8348
BamHI G'GATC_C
Cuts at: 504 1688 2934 5406 504
Size: 1184 1246 2472 3479
Fragments arranged by size:
3479 2472 1246 1184
BanI G'GyrC_C
Cuts at: 863 1169 1272 1849 2928 3181 3718 5397 5811
Size: 306 103 577 1079 253 537 1679 414
Cuts at: 5811 6059 6094 863
Size: 248 35 3150
Fragments arranged by size:
3150 1679 1079 577 537 414 306 253
248 103 35
BanII G_rGCy'C
Cuts at: 188 647 1412 2369 3123 4136 4547 5032 5144
Size: 459 765 957 754 1013 411 485 112
Cuts at: 5144 5395 6425 188
Size: 251 1030 2144
Fragments arranged by size:
2144 1030 1013 957 765 754 485 459
411 251 112
BbeI G_GCGC'C
Cuts at: 867 3185 5815 6063 867
Size: 2318 2630 248 3185
Fragments arranged by size:
3185 2630 2318 248
BbsI GAAGACnn'nnnn_
Cuts at: 1094 1588 1801 3565 4013 1094
Size: 494 213 1764 448 5462
Fragments arranged by size:
5462 1764 494 448 213
BbvCI CC'TCA_GC
Cuts at: 1811 1864 2386 4412 1811
Size: 53 522 2026 5780
Fragments arranged by size:
5780 2026 522 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 274 308 4195 4229 274
Size: 34 3887 34 4426
Fragments arranged by size:
4426 3887 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6279 7040 8381 6279
Size: 761 1341 6279
Fragments arranged by size:
6279 1341 761
BclI T'GATC_A
Cuts at: 5901 5901
Size: 8381
BglI GCCn_nnn'nGGC
Cuts at: 1741 3190 3229 1741
Size: 1449 39 6893
Fragments arranged by size:
6893 1449 39
BglII A'GATC_T
Cuts at: 476 2465 3911 5198 5896 7933 476
Size: 1989 1446 1287 698 2037 924
Fragments arranged by size:
2037 1989 1446 1287 924 698
BlpI GC'TnA_GC
Cuts at: 3379 5493 3379
Size: 2114 6267
Bme1580I G_kGCm'C
Cuts at: 647 1004 1277 2222 3048 3123 6006 6099 7155
Size: 357 273 945 826 75 2883 93 1056
Cuts at: 7155 647
Size: 1873
Fragments arranged by size:
2883 1873 1056 945 826 357 273 93
75
BmgBI CAC'GTC
Cuts at: 315 1092 1236 315
Size: 777 144 7460
Fragments arranged by size:
7460 777 144
BmrI ACTGGGnnnn_n'
Cuts at: 528 2351 5199 5593 5674 6009 8153 528
Size: 1823 2848 394 81 335 2144 756
Fragments arranged by size:
2848 2144 1823 756 394 335 81
BmtI G_CTAG'C
Cuts at: 3548 3548
Size: 8381
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 4152 5217 6748 2605
Size: 1547 1065 1531 4238
Fragments arranged by size:
4238 1547 1531 1065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 849 916 1726 1978 2218 2707 2789 2854 2965
Size: 67 810 252 240 489 82 65 111
Cuts at: 2965 3040 3070 3178 3325 3373 3706 3775 4102
Size: 75 30 108 147 48 333 69 327
Cuts at: 4102 4336 4417 4483 4555 4597 4696 4837 4858
Size: 234 81 66 72 42 99 141 21
Cuts at: 4858 4920 4972 849
Size: 62 52 4258
Fragments arranged by size:
4258 810 489 333 327 252 240 234
147 141 111 108 99 82 81 75
72 69 67 66 65 62 52 48
42 30 21
Bpu10I CC'TnA_GC
Cuts at: 302 1103 1811 1864 2386 3355 3859 4412 302
Size: 801 708 53 522 969 504 553 4271
Fragments arranged by size:
4271 969 801 708 553 522 504 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1039 1264 1288 1738 2895 2905 3385 3445 4324
Size: 225 24 450 1157 10 480 60 879
Cuts at: 4324 4648 4957 5557 6436 6928 7226 1039
Size: 324 309 600 879 492 298 2194
Fragments arranged by size:
2194 1157 879 879 600 492 480 450
324 309 298 225 60 24 10
BsaI GGTCTCn'nnnn_
Cuts at: 2392 2540 2734 3052 4543 4834 4883 8085 2392
Size: 148 194 318 1491 291 49 3202 2688
Fragments arranged by size:
3202 2688 1491 318 291 194 148 49
BsaAI yAC'GTr
Cuts at: 2331 2571 3084 5734 2331
Size: 240 513 2650 4978
Fragments arranged by size:
4978 2650 513 240
BsaBI GATnn'nnATC
Cuts at: 6 5895 7932 6
Size: 5889 2037 455
Fragments arranged by size:
5889 2037 455
BsaHI Gr'CG_yC
Cuts at: 864 3182 4033 4709 5812 6060 6762 8258 864
Size: 2318 851 676 1103 248 702 1496 987
Fragments arranged by size:
2318 1496 1103 987 851 702 676 248
BsaWI w'CCGG_w
Cuts at: 887 1166 1517 1889 3734 4556 5570 6091 6769
Size: 279 351 372 1845 822 1014 521 678
Cuts at: 6769 7043 7190 7601 887
Size: 274 147 411 1667
Fragments arranged by size:
1845 1667 1014 822 678 521 411 372
351 279 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2767 2797 3298 3328 3694 3724 4018 4048 4918
Size: 30 501 30 366 30 294 30 870
Cuts at: 4918 4948 2767
Size: 30 6200
Fragments arranged by size:
6200 870 501 366 294 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 550 619 922 1057 2695 2791 2824 2962 2998
Size: 69 303 135 1638 96 33 138 36
Cuts at: 2998 3028 3166 3193 3385 3388 3607 3610 3739
Size: 30 138 27 192 3 219 3 129
Cuts at: 3739 3880 4039 4072 4141 4144 4198 4201 4333
Size: 141 159 33 69 3 54 3 132
Cuts at: 4333 4693 4696 5209 550
Size: 360 3 513 3722
Fragments arranged by size:
3722 1638 513 360 303 219 192 159
141 138 138 135 132 129 96 69
69 54 36 33 33 30 27 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 853 1403 1423 1540 1979 2226 2480 3299 5806
Size: 550 20 117 439 247 254 819 2507
Cuts at: 5806 6564 7141 7637 853
Size: 758 577 496 1597
Fragments arranged by size:
2507 1597 819 758 577 550 496 439
254 247 117 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 756 910 1261 1744 3637 4180 4556 178
Size: 578 154 351 483 1893 543 376 4003
Fragments arranged by size:
4003 1893 578 543 483 376 351 154
BsiEI CG_ry'CG
Cuts at: 104 956 1448 5923 5969 7177 104
Size: 852 492 4475 46 1208 1308
Fragments arranged by size:
4475 1308 1208 852 492 46
BsiHKAI G_wGCw'C
Cuts at: 188 308 962 1004 1412 1807 1904 2155 2161
Size: 120 654 42 408 395 97 251 6
Cuts at: 2161 2186 2222 2309 2854 2921 3203 3461 4136
Size: 25 36 87 545 67 282 258 675
Cuts at: 4136 4547 4610 5395 6173 6363 7155 188
Size: 411 63 785 778 190 792 1414
Fragments arranged by size:
1414 792 785 778 675 654 545 411
408 395 282 258 251 190 120 97
87 67 63 42 36 25 6
BsmI GAATG_Cn'
Cuts at: 222 222
Size: 8381
BsmBI CGTCTCn'nnnn_
Cuts at: 3059 4040 3059
Size: 981 7400
Bsp1286I G_dGCh'C
Cuts at: 188 308 647 962 1004 1277 1412 1807 1904
Size: 120 339 315 42 273 135 395 97
Cuts at: 1904 2155 2161 2186 2222 2309 2369 2854 2921
Size: 251 6 25 36 87 60 485 67
Cuts at: 2921 3048 3123 3203 3461 4136 4547 4610 5032
Size: 127 75 80 258 675 411 63 422
Cuts at: 5032 5144 5395 6006 6099 6173 6363 6425 7155
Size: 112 251 611 93 74 190 62 730
Cuts at: 7155 188
Size: 1414
Fragments arranged by size:
1414 730 675 611 485 422 411 395
339 315 273 258 251 251 190 135
127 120 112 97 93 87 80 75
74 67 63 62 60 42 36 25
6
BspEI T'CCGG_A
Cuts at: 3734 4556 7601 3734
Size: 822 3045 4514
Fragments arranged by size:
4514 3045 822
BspHI T'CATG_A
Cuts at: 7557 7557
Size: 8381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 464 587 1037 1257 2247 2255 2432 4838 4901
Size: 123 450 220 990 8 177 2406 63
Cuts at: 4901 5075 5431 5645 5947 6328 464
Size: 174 356 214 302 381 2517
Fragments arranged by size:
2517 2406 990 450 381 356 302 220
214 177 174 123 63 8
BsrBI CCG'CTC
Cuts at: 50 1302 1740 2820 3420 4308 4335 6673 6727
Size: 1252 438 1080 600 888 27 2338 54
Cuts at: 6727 8211 8376 50
Size: 1484 165 55
Fragments arranged by size:
2338 1484 1252 1080 888 600 438 165
55 54 27
BsrDI GCAATG_nn'
Cuts at: 377 6293 377
Size: 5916 2465
BsrFI r'CCGG_y
Cuts at: 860 1166 1664 4289 4520 4721 6379 6560 6769
Size: 306 498 2625 231 201 1658 181 209
Cuts at: 6769 860
Size: 2472
Fragments arranged by size:
2625 2472 1658 498 306 231 209 201
181
BssSI C'ACGA_G
Cuts at: 1999 4886 6652 7010 1999
Size: 2887 1766 358 3370
Fragments arranged by size:
3370 2887 1766 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1248 1272 1908 2133 2632 4488 5758 1248
Size: 24 636 225 499 1856 1270 3871
Fragments arranged by size:
3871 1856 1270 636 499 225 24
BstBI TT'CG_AA
Cuts at: 6742 6742
Size: 8381
BstEII G'GTnAC_C
Cuts at: 4428 4428
Size: 8381
BstXI CCAn_nnnn'nTGG
Cuts at: 568 1147 1317 1659 1956 3612 3978 568
Size: 579 170 342 297 1656 366 4971
Fragments arranged by size:
4971 1656 579 366 342 297 170
BstYI r'GATC_y
Cuts at: 1 476 504 1688 2465 2934 3911 3989 4077
Size: 475 28 1184 777 469 977 78 88
Cuts at: 4077 4652 5198 5406 5873 5896 6231 6477 7489
Size: 575 546 208 467 23 335 246 1012
Cuts at: 7489 7575 7587 7933 1
Size: 86 12 346 449
Fragments arranged by size:
1184 1012 977 777 575 546 475 469
467 449 346 335 246 208 88 86
78 28 23 12
Bsu36I CC'TnA_GG
Cuts at: 1478 2891 3763 1478
Size: 1413 872 6096
Fragments arranged by size:
6096 1413 872
BtgI C'CryG_G
Cuts at: 1316 2078 2316 3425 4907 5033 1316
Size: 762 238 1109 1482 126 4664
Fragments arranged by size:
4664 1482 1109 762 238 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6331 6512 6675 6331
Size: 181 163 8037
Fragments arranged by size:
8037 181 163
BtsI GCAGTG_nn'
Cuts at: 1851 2155 2184 3025 5624 1851
Size: 304 29 841 2599 4608
Fragments arranged by size:
4608 2599 841 304 29
ClaI AT'CG_AT
Cuts at: 4232 4232
Size: 8381
DraI TTT'AAA
Cuts at: 5380 5380
Size: 8381
DraIII CAC_nnn'GTG
Cuts at: 600 1330 600
Size: 730 7651
DrdI GACnn_nn'nnGTC
Cuts at: 5320 6087 6945 5320
Size: 767 858 6756
Fragments arranged by size:
6756 858 767
EaeI y'GGCC_r
Cuts at: 374 653 953 1445 1649 1696 1946 1991 2075
Size: 279 300 492 204 47 250 45 84
Cuts at: 2075 2141 2165 2319 3221 3428 3479 3643 5036
Size: 66 24 154 902 207 51 164 1393
Cuts at: 5036 5966 6140 6531 6558 7903 374
Size: 930 174 391 27 1345 852
Fragments arranged by size:
1393 1345 930 902 852 492 391 300
279 250 207 204 174 164 154 84
66 51 47 45 27 24
EagI C'GGCC_G
Cuts at: 953 1445 5966 953
Size: 492 4521 3368
Fragments arranged by size:
4521 3368 492
EarI CTCTTCn'nnn_
Cuts at: 121 124 1957 2543 2612 3230 3235 4100 4505
Size: 3 1833 586 69 618 5 865 405
Cuts at: 4505 4790 5267 6404 6614 121
Size: 285 477 1137 210 1888
Fragments arranged by size:
1888 1833 1137 865 618 586 477 405
285 210 69 5 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 382 418 3142 3670 3673 4600 4927 6589 6899
Size: 36 2724 528 3 927 327 1662 310
Cuts at: 6899 7045 8191 382
Size: 146 1146 572
Fragments arranged by size:
2724 1662 1146 927 572 528 327 310
146 36 3
EcoICRI GAG'CTC
Cuts at: 186 1410 4134 4545 5393 186
Size: 1224 2724 411 848 3174
Fragments arranged by size:
3174 2724 1224 848 411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 457 849 916 1600 1726 1978 2218 2428
Size: 352 392 67 684 126 252 240 210
Cuts at: 2428 2669 2707 2789 2854 2965 3040 3070 3178
Size: 241 38 82 65 111 75 30 108
Cuts at: 3178 3254 3325 3373 3706 3775 3835 4102 4336
Size: 76 71 48 333 69 60 267 234
Cuts at: 4336 4417 4483 4555 4597 4696 4837 4858 4920
Size: 81 66 72 42 99 141 21 62
Cuts at: 4920 4972 6206 6638 7385 7653 105
Size: 52 1234 432 747 268 833
Fragments arranged by size:
1234 833 747 684 432 392 352 333
268 267 252 241 240 234 210 141
126 111 108 99 82 81 76 75
72 71 69 67 66 65 62 60
52 48 42 38 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 325 655 2116 3052 4678 5099 325
Size: 330 1461 936 1626 421 3607
Fragments arranged by size:
3607 1626 1461 936 421 330
EcoO109I rG'GnC_Cy
Cuts at: 644 1350 1482 1749 1833 1868 2887 3096 3119
Size: 706 132 267 84 35 1019 209 23
Cuts at: 3119 3759 3775 3939 4461 5212 5277 5520 644
Size: 640 16 164 522 751 65 243 3505
Fragments arranged by size:
3505 1019 751 706 640 522 267 243
209 164 132 84 65 35 23 16
EcoRI G'AATT_C
Cuts at: 4750 5385 4750
Size: 635 7746
EcoRV GAT'ATC
Cuts at: 426 426
Size: 8381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2647 4753 6400 2647
Size: 2106 1647 4628
Fragments arranged by size:
4628 2106 1647
FspI TGC'GCA
Cuts at: 1117 3103 6162 1117
Size: 1986 3059 3336
Fragments arranged by size:
3336 3059 1986
HaeII r_GCGC'y
Cuts at: 771 867 1424 2205 2291 3185 3345 4290 4396
Size: 96 557 781 86 894 160 945 106
Cuts at: 4396 5025 5076 5815 6063 7085 8064 771
Size: 629 51 739 248 1022 979 1088
Fragments arranged by size:
1088 1022 979 945 894 781 739 629
557 248 160 106 96 86 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1575 1607 4018 4050 5233 5265 5289 5321 6223
Size: 32 2411 32 1183 32 24 32 902
Cuts at: 6223 6253 6255 6285 1575
Size: 30 2 30 3671
Fragments arranged by size:
3671 2411 1183 902 32 32 32 32
30 30 24 2
HincII GTy'rAC
Cuts at: 516 5325 5420 516
Size: 4809 95 3477
Fragments arranged by size:
4809 3477 95
KasI G'GCGC_C
Cuts at: 863 3181 5811 6059 863
Size: 2318 2630 248 3185
Fragments arranged by size:
3185 2630 2318 248
KpnI G_GTAC'C
Cuts at: 1173 5401 1173
Size: 4228 4153
MluI A'CGCG_T
Cuts at: 7814 7814
Size: 8381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 314 4518 7052 7236 314
Size: 4204 2534 184 1459
Fragments arranged by size:
4204 2534 1459 184
MscI TGG'CCA
Cuts at: 655 1651 1948 1993 2167 6142 655
Size: 996 297 45 174 3975 2894
Fragments arranged by size:
3975 2894 996 297 174 45
MslI CAynn'nnrTG
Cuts at: 1315 2608 4234 4906 5161 6370 6497 1315
Size: 1293 1626 672 255 1209 127 3199
Fragments arranged by size:
3199 1626 1293 1209 672 255 127
MspA1I CmG'CkG
Cuts at: 207 435 585 945 1540 1566 1626 1914 2318
Size: 228 150 360 595 26 60 288 404
Cuts at: 2318 2832 2964 2970 3003 3033 3039 3144 3303
Size: 514 132 6 33 30 6 105 159
Cuts at: 3303 3324 3855 3921 4314 4371 4383 4503 4815
Size: 21 531 66 393 57 12 120 312
Cuts at: 4815 4836 5055 5492 5806 6166 7179 7424 207
Size: 21 219 437 314 360 1013 245 1164
Fragments arranged by size:
1164 1013 595 531 514 437 404 393
360 360 314 312 288 245 228 219
159 150 132 120 105 66 60 57
33 30 26 21 21 12 6 6
NaeI GCC'GGC
Cuts at: 862 4291 4723 6562 862
Size: 3429 432 1839 2681
Fragments arranged by size:
3429 2681 1839 432
NarI GG'CG_CC
Cuts at: 864 3182 5812 6060 864
Size: 2318 2630 248 3185
Fragments arranged by size:
3185 2630 2318 248
NcoI C'CATG_G
Cuts at: 2078 3425 2078
Size: 1347 7034
NgoMIV G'CCGG_C
Cuts at: 860 4289 4721 6560 860
Size: 3429 432 1839 2681
Fragments arranged by size:
3429 2681 1839 432
NheI G'CTAG_C
Cuts at: 3544 3544
Size: 8381
NspI r_CATG'y
Cuts at: 765 819 4486 5160 5434 5934 6841 765
Size: 54 3667 674 274 500 907 2305
Fragments arranged by size:
3667 2305 907 674 500 274 54
PciI A'CATG_T
Cuts at: 5156 6837 5156
Size: 1681 6700
PflMI CCAn_nnn'nTGG
Cuts at: 600 948 1269 1846 2259 4943 5349 600
Size: 348 321 577 413 2684 406 3632
Fragments arranged by size:
3632 2684 577 413 406 348 321
PfoI T'CCnGG_A
Cuts at: 1593 2048 2177 3389 3983 4532 5342 1593
Size: 455 129 1212 594 549 810 4632
Fragments arranged by size:
4632 1212 810 594 549 455 129
PmeI GTTT'AAAC
Cuts at: 5380 5380
Size: 8381
PmlI CAC'GTG
Cuts at: 2331 2571 2331
Size: 240 8141
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7520 7552 7520
Size: 32 8349
PpuMI rG'GwC_Cy
Cuts at: 1749 1868 3096 4461 5212 1749
Size: 119 1228 1365 751 4918
Fragments arranged by size:
4918 1365 1228 751 119
PshAI GACnn'nnGTC
Cuts at: 4944 4944
Size: 8381
PspOMI G'GGCC_C
Cuts at: 643 3119 643
Size: 2476 5905
PspXI vC'TCGA_Gb
Cuts at: 2154 2154
Size: 8381
PstI C_TGCA'G
Cuts at: 212 287 326 755 1253 1385 1562 1913 1973
Size: 75 39 429 498 132 177 351 60
Cuts at: 1973 2339 2681 2969 3011 3032 3038 3212 3290
Size: 366 342 288 42 21 6 174 78
Cuts at: 3290 3329 4286 4457 4835 5018 5285 5428 6113
Size: 39 957 171 378 183 267 143 685
Cuts at: 6113 212
Size: 2480
Fragments arranged by size:
2480 957 685 498 429 378 366 351
342 288 267 183 177 174 171 143
132 78 75 60 42 39 39 21
6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8381
PvuII CAG'CTG
Cuts at: 207 435 945 1540 1626 1914 2832 2964 3003
Size: 228 510 595 86 288 918 132 39
Cuts at: 3003 3033 3039 3144 3303 3324 3921 4314 4503
Size: 30 6 105 159 21 597 393 189
Cuts at: 4503 4815 4836 5806 6166 207
Size: 312 21 970 360 2422
Fragments arranged by size:
2422 970 918 597 595 510 393 360
312 288 228 189 159 132 105 86
39 30 21 21 6
RsrII CG'GwC_CG
Cuts at: 6576 6576
Size: 8381
SacI G_AGCT'C
Cuts at: 188 1412 4136 4547 5395 188
Size: 1224 2724 411 848 3174
Fragments arranged by size:
3174 2724 1224 848 411
SacII CC_GC'GG
Cuts at: 2319 2319
Size: 8381
SalI G'TCGA_C
Cuts at: 5418 5418
Size: 8381
SanDI GG'GwC_CC
Cuts at: 5212 5212
Size: 8381
SapI GCTCTTCn'nnn_
Cuts at: 124 1957 3235 4100 4505 6404 6614 124
Size: 1833 1278 865 405 1899 210 1891
Fragments arranged by size:
1899 1891 1833 1278 865 405 210
SbfI CC_TGCA'GG
Cuts at: 5428 5428
Size: 8381
ScaI AGT'ACT
Cuts at: 7898 7898
Size: 8381
SexAI A'CCwGG_T
Cuts at: 224 224
Size: 8381
SfcI C'TryA_G
Cuts at: 33 208 283 322 480 751 1249 1368 1381
Size: 175 75 39 158 271 498 119 13
Cuts at: 1381 1558 1612 1909 1969 2281 2335 2677 2965
Size: 177 54 297 60 312 54 342 288
Cuts at: 2965 3007 3028 3034 3208 3286 3325 4282 4453
Size: 42 21 6 174 78 39 957 171
Cuts at: 4453 4831 5014 5281 5424 6109 7102 7293 33
Size: 378 183 267 143 685 993 191 1121
Fragments arranged by size:
1121 993 957 685 498 378 342 312
297 288 271 267 191 183 177 175
174 171 158 143 119 78 75 60
54 54 42 39 39 21 13 6
SfoI GGC'GCC
Cuts at: 865 3183 5813 6061 865
Size: 2318 2630 248 3185
Fragments arranged by size:
3185 2630 2318 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8381
SgrAI Cr'CCGG_yG
Cuts at: 860 4289 4520 860
Size: 3429 231 4721
Fragments arranged by size:
4721 3429 231
SmaI CCC'GGG
Cuts at: 966 3187 5403 966
Size: 2221 2216 3944
Fragments arranged by size:
3944 2221 2216
SmlI C'TyrA_G
Cuts at: 1054 1279 1303 1753 2154 2874 2920 3400 3460
Size: 225 24 450 401 720 46 480 60
Cuts at: 3460 4303 4546 4663 4972 4999 5536 6451 6943
Size: 843 243 117 309 27 537 915 492
Cuts at: 6943 7205 1054
Size: 262 2230
Fragments arranged by size:
2230 915 843 720 537 492 480 450
401 309 262 243 225 117 60 46
27 24
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8381
SphI G_CATG'C
Cuts at: 819 4486 5434 5934 819
Size: 3667 948 500 3266
Fragments arranged by size:
3667 3266 948 500
StuI AGG'CCT
Cuts at: 4196 4196
Size: 8381
StyI C'CwwG_G
Cuts at: 656 806 1745 2078 2291 3425 3533 3587 3653
Size: 150 939 333 213 1134 108 54 66
Cuts at: 3653 4019 4772 4985 5515 5869 656
Size: 366 753 213 530 354 3168
Fragments arranged by size:
3168 1134 939 753 530 366 354 333
213 213 150 108 66 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 413 1600 2104 2869 5964 6097 6556 6765 413
Size: 1187 504 765 3095 133 459 209 2029
Fragments arranged by size:
3095 2029 1187 765 504 459 209 133
TatI w'GTAC_w
Cuts at: 785 1058 2457 3818 7896 785
Size: 273 1399 1361 4078 1270
Fragments arranged by size:
4078 1399 1361 1270 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2767 2799 7898 7930 2767
Size: 32 5099 32 3218
Fragments arranged by size:
5099 3218 32 32
Tth111I GACn'n_nGTC
Cuts at: 6178 6178
Size: 8381
XbaI T'CTAG_A
Cuts at: 5412 5412
Size: 8381
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 515 4026 5346 5876 515
Size: 3511 1320 530 3020
Fragments arranged by size:
3511 3020 1320 530
XhoI C'TCGA_G
Cuts at: 2154 4546 4999 2154
Size: 2392 453 5536
Fragments arranged by size:
5536 2392 453
XmaI C'CCGG_G
Cuts at: 964 3185 5401 964
Size: 2221 2216 3944
Fragments arranged by size:
3944 2221 2216
XmnI GAAnn'nnTTC
Cuts at: 5158 5158
Size: 8381
ZraI GAC'GTC
Cuts at: 4710 4710
Size: 8381
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AloI AscI AvrII BfrBI BsiWI BsrGI BssHII
BstZ17I CspCI FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PsiI PsrI SfiI
SnaBI SrfI SspI SwaI